##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568671_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 128094 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19559854481865 33.0 31.0 34.0 30.0 34.0 2 32.39257888737958 34.0 31.0 34.0 30.0 34.0 3 32.409902103143004 34.0 31.0 34.0 30.0 34.0 4 35.90556154074352 37.0 35.0 37.0 35.0 37.0 5 35.953901041422704 37.0 35.0 37.0 35.0 37.0 6 36.00457476540665 37.0 35.0 37.0 35.0 37.0 7 35.84936843255734 37.0 35.0 37.0 35.0 37.0 8 35.88007244679688 37.0 35.0 37.0 35.0 37.0 9 37.67959467266226 39.0 37.0 39.0 35.0 39.0 10-11 37.54410042624947 39.0 37.0 39.0 35.0 39.0 12-13 37.49796243383765 39.0 37.0 39.0 34.5 39.0 14-15 38.57760316642465 40.0 38.0 41.0 34.0 41.0 16-17 38.40261448623667 40.0 38.0 41.0 33.5 41.0 18-19 38.61073898855528 40.0 38.0 41.0 34.0 41.0 20-21 38.78467375521102 40.0 38.0 41.0 34.0 41.0 22-23 38.72604493575031 40.0 38.0 41.0 34.0 41.0 24-25 38.446301153840146 40.0 38.0 41.0 34.0 41.0 26-27 38.15453104751198 40.0 37.5 41.0 33.5 41.0 28-29 38.03783549580777 40.0 37.5 41.0 33.0 41.0 30-31 38.04942073789561 40.0 37.0 41.0 33.0 41.0 32-33 37.52055521726233 40.0 36.0 41.0 32.5 41.0 34-35 37.08449654160226 39.0 35.5 41.0 31.0 41.0 36-37 37.00195949849329 39.5 35.5 41.0 30.5 41.0 38-39 36.10061361187877 38.5 35.0 40.0 29.5 41.0 40-41 36.27228051274845 39.0 35.0 40.0 29.5 41.0 42-43 36.236322544381466 39.0 35.0 41.0 29.0 41.0 44-45 35.989894140240764 39.0 35.0 40.5 27.0 41.0 46-47 35.5064640029978 39.0 34.5 40.5 25.5 41.0 48-49 35.34661264383968 39.0 34.5 40.5 24.5 41.0 50-51 34.453178915483946 38.0 32.5 40.0 21.0 41.0 52-53 35.192428997454996 39.0 35.0 41.0 22.5 41.0 54-55 34.91500772869924 39.0 34.5 40.5 22.0 41.0 56-57 33.89656424188487 38.0 32.5 40.0 19.0 41.0 58-59 33.23921885490343 38.0 31.5 39.0 12.5 41.0 60-61 31.708436772994833 36.0 28.5 39.0 9.0 41.0 62-63 30.52142176838884 35.0 26.0 39.0 8.0 41.0 64-65 29.154632535481756 35.0 21.5 37.5 7.0 40.5 66-67 26.851706559245557 32.0 15.0 37.0 7.0 39.5 68-69 24.2023474948085 27.0 9.5 36.0 7.0 39.0 70-71 22.81787593486034 24.0 8.0 35.5 7.0 38.0 72-73 22.167552734710448 23.0 8.0 35.0 7.0 37.0 74-75 21.970685590269646 23.5 7.0 35.0 7.0 37.0 76-77 21.278896747700905 22.5 8.0 33.5 7.0 35.0 78-79 22.03428341686574 25.0 7.0 35.0 7.0 35.0 80-81 22.023943354099334 25.0 7.0 35.0 7.0 35.0 82-83 21.812992802160913 24.5 7.0 35.0 7.0 35.0 84-85 21.604829266007776 24.0 7.0 35.0 7.0 35.0 86-87 21.526757693568783 24.0 7.0 35.0 7.0 35.0 88-89 21.407005792621042 23.0 7.0 35.0 7.0 35.0 90-91 21.32109622620888 23.0 7.0 35.0 7.0 35.0 92-93 21.263302730807062 23.0 7.0 35.0 7.0 35.0 94-95 21.205029119240557 23.0 7.0 35.0 7.0 35.0 96-97 21.147403469327212 23.0 7.0 35.0 7.0 35.0 98-99 21.11813199681484 23.0 7.0 35.0 7.0 35.0 100-101 20.257642824800538 19.5 7.0 33.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.464072485282081 1101 2 0.18268652631467575 1101 3 0.20931082205466112 1101 4 0.41249014060474565 1101 5 0.0238939124237163 1101 6 0.09763075987552838 1101 7 0.041386524465657715 1101 8 0.09895450942293138 1101 9 -0.07099272167009474 1101 10-11 -0.08835630866033739 1101 12-13 0.09082987747912341 1101 14-15 0.30477743291539383 1101 16-17 0.08171002839583963 1101 18-19 0.2214412777635033 1101 20-21 0.1260415507433379 1101 22-23 -0.04277034328291052 1101 24-25 0.10165626779005521 1101 26-27 0.6292157209318745 1101 28-29 0.6003592581033672 1101 30-31 0.4603264867411667 1101 32-33 0.4923355454308975 1101 34-35 0.53266215005155 1101 36-37 0.47667898712542467 1101 38-39 0.4110757710015207 1101 40-41 -0.013706071003738884 1101 42-43 -0.14597076899516281 1101 44-45 0.6250884294734291 1101 46-47 0.44948643847192216 1101 48-49 0.4579133341910975 1101 50-51 1.1782838283431332 1101 52-53 0.8039768880402605 1101 54-55 1.072409015397973 1101 56-57 1.50688425000979 1101 58-59 0.48423279574741684 1101 60-61 0.2959234969733018 1101 62-63 1.1412066829860912 1101 64-65 -0.2580218682440787 1101 66-67 0.1981216264485397 1101 68-69 0.8365050388085074 1101 70-71 0.7986659577800239 1101 72-73 1.771991454203139 1101 74-75 1.3498929654925966 1101 76-77 0.9627321608834976 1101 78-79 1.7800479987363858 1101 80-81 1.4253008714160273 1101 82-83 0.8635661866296473 1101 84-85 1.2846780543724527 1101 86-87 0.8864199236803074 1101 88-89 0.9471518884070633 1101 90-91 1.8136420175424206 1101 92-93 0.8540156653740922 1101 94-95 1.55830614045232 1101 96-97 1.0351592443886481 1101 98-99 1.327583548933891 1101 100-101 1.1231381890601746 1102 1 0.14569641690601287 1102 2 -0.1018576332294856 1102 3 -0.04425043150659036 1102 4 -0.08466085654625033 1102 5 -0.22646221293241098 1102 6 0.015721927966694693 1102 7 0.01859450167363974 1102 8 0.050521460989884304 1102 9 0.37594460026722487 1102 10-11 0.24266220985818165 1102 12-13 0.2881233247725703 1102 14-15 0.2493999400379039 1102 16-17 -0.007321310635504119 1102 18-19 -0.09533222650999562 1102 20-21 0.6775016647034562 1102 22-23 0.44156014104757446 1102 24-25 0.44086566949945905 1102 26-27 0.28840375511990857 1102 28-29 0.5668834746275806 1102 30-31 0.14284073175541323 1102 32-33 -0.10274992465457444 1102 34-35 -0.2280216106322115 1102 36-37 0.07265477060121128 1102 38-39 -0.007905710479960248 1102 40-41 0.20424264694498362 1102 42-43 0.4199124218880286 1102 44-45 0.3900456944306967 1102 46-47 -0.21557766409218715 1102 48-49 -0.33143851766075016 1102 50-51 0.2146086146679167 1102 52-53 0.48684725841062715 1102 54-55 0.16215260514156427 1102 56-57 -0.30312287249733316 1102 58-59 -0.2588227598081403 1102 60-61 -0.8451021440523334 1102 62-63 -0.1882092714298622 1102 64-65 0.4358883881661768 1102 66-67 -0.3086447553178395 1102 68-69 -1.0436587788553098 1102 70-71 0.032640316754381615 1102 72-73 0.678330485542169 1102 74-75 0.02136732446695433 1102 76-77 0.5051110782624164 1102 78-79 0.14009073377912173 1102 80-81 -0.38150112288596816 1102 82-83 0.487319747883209 1102 84-85 0.15202135921575533 1102 86-87 0.9969968467572308 1102 88-89 1.3064823727375483 1102 90-91 0.2287061201065228 1102 92-93 0.9458960072544329 1102 94-95 0.8195240891702653 1102 96-97 1.1971962814256862 1102 98-99 0.7779040617544055 1102 100-101 0.5795128329348209 1103 1 0.32366844487803803 1103 2 -0.04643805280990421 1103 3 -0.011732948989106262 1103 4 0.21568879380339467 1103 5 -0.09411955558974938 1103 6 0.18635129859606536 1103 7 0.48852457160370477 1103 8 0.3809410414094643 1103 9 0.2540914534140768 1103 10-11 -0.006201426505455743 1103 12-13 -0.01607247942323653 1103 14-15 0.4650467931847544 1103 16-17 0.3124339341197455 1103 18-19 0.38709784342007225 1103 20-21 0.40398767868946805 1103 22-23 0.26690979139722515 1103 24-25 0.12312091425470584 1103 26-27 0.29259955931571824 1103 28-29 0.020729628473731054 1103 30-31 0.3240470254617094 1103 32-33 0.09183049492584416 1103 34-35 -0.1918327994433966 1103 36-37 0.044944980391420586 1103 38-39 -0.16586025593450415 1103 40-41 -0.5508272831249457 1103 42-43 0.2797026316782407 1103 44-45 -0.5819822775972767 1103 46-47 0.24115310513858645 1103 48-49 -0.062120335842571706 1103 50-51 -0.6388878888285845 1103 52-53 0.41980180386517674 1103 54-55 0.16634840933737394 1103 56-57 -0.5283851102595705 1103 58-59 -0.23950457798996183 1103 60-61 -0.36013710908729735 1103 62-63 -0.9598875931081814 1103 64-65 -1.0905976258198322 1103 66-67 -0.905497902170989 1103 68-69 -1.6974175201140547 1103 70-71 -1.2596673755533132 1103 72-73 -0.7928233606116777 1103 74-75 -1.5175312769316491 1103 76-77 -1.5618469636956256 1103 78-79 -1.4106085669201782 1103 80-81 -1.4842109130957581 1103 82-83 -2.0267536786902163 1103 84-85 -2.5230660533716573 1103 86-87 -2.022670985410599 1103 88-89 -1.3658952496400723 1103 90-91 -1.8075701036697005 1103 92-93 -1.2212368598784344 1103 94-95 -2.4197241625779853 1103 96-97 -1.909621900392498 1103 98-99 -1.3443861480358024 1103 100-101 -1.6880570971351112 1104 1 0.14185026305985815 1104 2 0.15474771794108477 1104 3 -0.24809658535274082 1104 4 -0.08549697694758862 1104 5 -0.07989030657789442 1104 6 -0.09230482788615291 1104 7 -0.1355861003330503 1104 8 -0.14396014436128723 1104 9 -0.1783196137795997 1104 10-11 -0.01766387709834305 1104 12-13 0.03946111741470304 1104 14-15 -0.03475557835675147 1104 16-17 0.15195962581934452 1104 18-19 0.13255238887461473 1104 20-21 0.258730761693414 1104 22-23 0.4048544554288469 1104 24-25 0.2626466059543091 1104 26-27 0.42184857117342034 1104 28-29 -0.12492254543930414 1104 30-31 0.41278220332732474 1104 32-33 -0.29196397147731545 1104 34-35 0.06152688435106768 1104 36-37 -0.036082687592767115 1104 38-39 0.025246463433077793 1104 40-41 -0.4923292594095372 1104 42-43 -0.38175981891464517 1104 44-45 0.14706910580193266 1104 46-47 -0.17841211225272247 1104 48-49 -0.5200254741824892 1104 50-51 -0.5422475726230473 1104 52-53 -0.6778266546328524 1104 54-55 -0.6152721440223061 1104 56-57 -1.1677131735006796 1104 58-59 -0.9213227598081417 1104 60-61 -1.6874098363600254 1104 62-63 -0.7385437195903997 1104 64-65 -1.1344711436063974 1104 66-67 -1.1313872302342283 1104 68-69 -1.8966270062800596 1104 70-71 -1.5748847668576573 1104 72-73 -2.1623885780029823 1104 74-75 -1.551918628710304 1104 76-77 -1.6325979518379192 1104 78-79 -0.3973674602008117 1104 80-81 -0.6021951028190777 1104 82-83 -0.45402640596294574 1104 84-85 -0.8934217845969528 1104 86-87 -0.15271051110229905 1104 88-89 -0.18565809548987744 1104 90-91 0.051323176962707606 1104 92-93 -0.6761775713408831 1104 94-95 -0.40292574360565325 1104 96-97 -0.5125863272699682 1104 98-99 0.1735980416875158 1104 100-101 -0.04260255168056659 1105 1 0.2686359773455749 1105 2 0.0719061030342516 1105 3 0.46261770036154104 1105 4 0.28484463795923887 1105 5 0.22958174311154522 1105 6 0.1469032466480087 1105 7 0.08040768848682234 1105 8 -0.052500517032093796 1105 9 0.44256547938810087 1105 10-11 0.35426935271532045 1105 12-13 0.1844145335637819 1105 14-15 0.1553065334445023 1105 16-17 0.476881986067788 1105 18-19 0.5691595317317635 1105 20-21 0.4041500163518066 1105 22-23 0.5744584926959249 1105 24-25 0.5634618233456123 1105 26-27 0.6778268320429888 1105 28-29 0.7341224856165951 1105 30-31 0.6881704020850847 1105 32-33 0.7011649105102578 1105 34-35 0.3774042135436133 1105 36-37 0.5661300453264886 1105 38-39 1.4206657180914704 1105 40-41 1.2526629766153121 1105 42-43 0.9937448394704447 1105 44-45 1.1424495405845434 1105 46-47 0.9018673908528783 1105 48-49 0.8873114823392498 1105 50-51 0.957053669612975 1105 52-53 1.192204401267766 1105 54-55 1.3908613963503527 1105 56-57 0.8884980066235428 1105 58-59 0.9286772401918597 1105 60-61 0.6768758779256885 1105 62-63 0.7060214978009078 1105 64-65 1.2672757508035417 1105 66-67 0.9945832666601824 1105 68-69 1.4618357266391975 1105 70-71 2.059082624446688 1105 72-73 2.104498067959753 1105 74-75 2.065666775016407 1105 76-77 1.695701737603077 1105 78-79 2.1644726019109903 1105 80-81 2.0137598661250244 1105 82-83 2.092402165465625 1105 84-85 2.028394985589383 1105 86-87 2.0069556379660227 1105 88-89 2.5420592958144717 1105 90-91 2.0978682080186104 1105 92-93 2.732983919342349 1105 94-95 2.275568045214225 1105 96-97 2.4471962814256862 1105 98-99 2.1862145013148435 1105 100-101 1.6859688768908647 1106 1 0.30851692972652955 1106 2 0.15583467446282384 1106 3 0.1852367479805892 1106 4 0.30508273319733803 1106 5 0.333153171682973 1106 6 0.30226038950515743 1106 7 0.2839791170582515 1106 8 0.3998804353488552 1106 9 0.3419702412928629 1106 10-11 0.36766220985818165 1106 12-13 0.4820335811828187 1106 14-15 0.5445922477302076 1106 16-17 0.3881915098773163 1106 18-19 0.5658857222079519 1106 20-21 0.4255785877803788 1106 22-23 0.4021370641244957 1106 24-25 0.15304515667894947 1106 26-27 0.17782683204298877 1106 28-29 0.24572962847373958 1106 30-31 0.3691227830374686 1106 32-33 0.5770577676531161 1106 34-35 0.59377326116266 1106 36-37 0.38547528342172654 1106 38-39 0.5087609561867055 1106 40-41 0.49879392899625685 1106 42-43 -0.1964337319581304 1106 44-45 -0.16380045941545518 1106 46-47 -0.18801356152808069 1106 48-49 -0.402271850994083 1106 50-51 -0.37866061610130686 1106 52-53 -0.09231940825603857 1106 54-55 -0.6275909846020156 1106 56-57 -1.0620972314716894 1106 58-59 -1.2296560931414717 1106 60-61 -1.3124098363600254 1106 62-63 -0.8981451688657565 1106 64-65 -1.8080218682440758 1106 66-67 -1.2092857809588686 1106 68-69 -1.5584023685989017 1106 70-71 -0.7846673755533118 1106 72-73 -1.2344900272783441 1106 74-75 -1.2917737011740726 1106 76-77 -1.2557863576350208 1106 78-79 -0.8268964457080585 1106 80-81 -1.2311806100654543 1106 82-83 -0.9290264059629436 1106 84-85 -1.270414538220141 1106 86-87 -0.5927467429863569 1106 88-89 -1.0621073708521926 1106 90-91 -1.5238579824575815 1106 92-93 -1.5682065568481285 1106 94-95 -0.7333605262143479 1106 96-97 -0.8944703852409823 1106 98-99 -1.0724164510661076 1106 100-101 -0.3884358850139016 1107 1 0.13680824625313903 1107 2 -0.015803980999365308 1107 3 0.047701733974989224 1107 4 0.2144664866987398 1107 5 0.10878342378381944 1107 6 0.22936122984128815 1107 7 0.15918920109186985 1107 8 0.1082137686821909 1107 9 0.05653606762339791 1107 10-11 0.0604143106985191 1107 12-13 0.2679229369251175 1107 14-15 0.504046029242815 1107 16-17 0.48791139783249804 1107 18-19 0.23591373341243127 1107 20-21 0.10845673904087505 1107 22-23 0.07577151790600567 1107 24-25 -0.32493103379724175 1107 26-27 -0.18509333602423794 1107 28-29 -0.17632919505567912 1107 30-31 -0.3711783374107114 1107 32-33 0.09060818782118929 1107 34-35 0.04048194463604915 1107 36-37 -0.4178510471104886 1107 38-39 -0.42754856762282145 1107 40-41 -0.27596097296451916 1107 42-43 -0.24233709330265896 1107 44-45 -0.702876089667555 1107 46-47 -0.5340569788950305 1107 48-49 -0.12082927396327392 1107 50-51 0.2737553502852492 1107 52-53 -0.07775358192550641 1107 54-55 0.11932778290498192 1107 56-57 0.46622909906052 1107 58-59 0.7206940469145451 1107 60-61 0.27981705439627547 1107 62-63 0.2932063717504896 1107 64-65 0.46921902811446614 1107 66-67 -0.38330538880200393 1107 68-69 -0.20360544983139306 1107 70-71 0.28781161604332794 1107 72-73 0.492890925102607 1107 74-75 0.7318957666130395 1107 76-77 0.3377710653341701 1107 78-79 -0.05900428884531195 1107 80-81 0.8055140678056922 1107 82-83 0.22160384613789574 1107 84-85 0.3209370023961071 1107 86-87 0.6511258060332494 1107 88-89 0.7274584554783381 1107 90-91 0.6259669475144101 1107 92-93 0.715126776485203 1107 94-95 1.455715104037754 1107 96-97 1.3053895587366107 1107 98-99 1.6213510559366924 1107 100-101 0.9380697172269983 1108 1 0.09033511154471086 1108 2 0.09598618961433658 1108 3 -0.04658143383759494 1108 4 0.1490221271367318 1108 5 0.08618347471328036 1108 6 0.015139177383943547 1108 7 0.08852457160370619 1108 8 -0.12663471616629352 1108 9 -0.17621157688895295 1108 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-0.15481395552243526 2316 100-101 -0.13030308643992328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 3.0 14 23.0 15 97.0 16 275.0 17 582.0 18 1017.0 19 1425.0 20 2106.0 21 2949.0 22 3333.0 23 3334.0 24 3908.0 25 5325.0 26 7551.0 27 9387.0 28 12717.0 29 12755.0 30 6183.0 31 2444.0 32 1998.0 33 2528.0 34 2813.0 35 3718.0 36 6862.0 37 17370.0 38 16794.0 39 593.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.263846267969107 22.751372328546775 16.143112589503932 30.841668813980185 2 8.895748671459831 18.68005626758362 52.20850265708034 20.21569240387621 3 14.144300279482255 33.91884085124987 42.5406342217434 9.396224647524475 4 7.18300622979999 32.84462972504567 23.05416334879073 36.918200696363606 5 6.075226005901916 38.92532046778147 38.98621324964479 16.01324027667182 6 18.808503329664067 42.54084986454942 14.855844672927839 23.79480213285867 7 13.532824713608571 36.35824112323226 13.327450628226051 36.781483534933116 8 22.332037410027013 35.9439161865505 23.92383718206942 17.800209221353068 9 18.33965681452683 12.235545771074367 11.952160132402767 57.47263728199603 10-11 16.462910050431713 27.274501537933084 28.19374834106203 28.068840070573174 12-13 25.63273845769513 20.224600683872783 23.644745265195873 30.49791559323622 14-15 25.27362718003966 22.713007635018034 16.586647305884743 35.42671787905757 16-17 25.53515387137571 33.17680765687698 16.64597873436695 24.64205973738036 18-19 24.654940902774523 22.971801957937142 18.303355348416005 34.06990179087232 20-21 21.24728714850032 17.87593486033694 19.5735163239496 41.30326166721314 22-23 17.074570237481847 32.45663340983965 19.196059144066076 31.272737208612426 24-25 23.631864099801707 34.596077880306645 20.377222976876354 21.394835043015288 26-27 22.490514778209754 27.23898074851281 26.12690680281668 24.143597670460757 28-29 11.654813299711927 16.91427322336115 29.610127017089145 41.82078645983777 30-31 13.291801333395787 16.818898621324966 35.59495370587225 34.294346339407 32-33 12.5165893796743 23.187268724530423 36.018080472153265 28.278061423642015 34-35 11.036426374381314 32.58271269536434 38.48814152107046 17.89271940918388 36-37 15.07447655627898 27.20853435758115 25.56247755554515 32.15451153059472 38-39 18.524677190188456 17.686230424531985 32.147875778725 31.64121660655456 40-41 14.326197948381658 20.095789029931144 37.92644464221587 27.651568379471325 42-43 24.943791278280013 17.849782191203335 38.683701032054586 18.522725498462066 44-45 14.38826174528081 23.625228347931987 35.942745171514666 26.043764735272536 46-47 26.812731275469577 15.81885178072353 27.562961575093293 29.805455368713602 48-49 21.61342451637079 15.928927194091838 31.78290942589036 30.67473886364701 50-51 12.71566193576592 19.84363045888176 38.33473855137633 29.10596905397599 52-53 13.431152122659922 23.576436054772277 43.66402797945259 19.328383843115212 54-55 11.995097350383311 22.835573875435227 33.22130622823864 31.948022545942823 56-57 24.361406467125704 25.404780864052963 22.252018049245088 27.98179461957625 58-59 13.778553249957062 33.793542242415725 35.39861351819758 17.029290989429636 60-61 20.711742938779334 45.639530344902965 17.402064109169828 16.246662607147876 62-63 10.616073415122546 55.464562994999746 15.715083122875088 18.20428046700262 64-65 10.667946976439177 55.347635330304314 15.218901744031726 18.76551594922479 66-67 11.928349493340829 50.996924133839215 15.807922307055755 21.266804065764205 68-69 12.924492950489485 46.41474229862445 18.75848985900979 21.902274891876278 70-71 14.811388511561821 43.602354520898714 19.757756022920667 21.828500944618796 72-73 14.85705809795931 39.143909941136975 21.965509703811264 24.03352225709245 74-75 14.504972910518838 38.08257998032695 22.85391977766328 24.558527331490936 76-77 14.918341218167907 36.004028291723266 23.510078536075067 25.567551954033757 78-79 15.26535200711977 35.19212453354568 24.321591955907383 25.220931503427167 80-81 16.59718644120724 33.96919449779068 23.817665152153886 25.615953908848187 82-83 16.06203256983153 32.709572657579585 25.393070713694634 25.835324058894248 84-85 16.17015629147345 31.828188673942577 25.095632894593034 26.90602213999094 86-87 16.87198463628273 30.860930254344467 24.103392820897156 28.16369228847565 88-89 17.06598279388574 29.88430371445969 24.228691429731292 28.821022061923273 90-91 18.204209408715474 27.708948116227145 24.8145892859931 29.27225318906428 92-93 17.848611176167502 28.010289318781517 24.57531188033788 29.565787624713103 94-95 17.850172529548615 27.36740206410917 25.353256202476302 29.42916920386591 96-97 16.76776429809359 27.326026199509734 26.497337892485206 29.40887160991147 98-99 18.141755273471045 27.98335597295736 24.183412181679078 29.691476571892515 100-101 17.69144293599182 28.631931487278774 24.056349683433133 29.620275893296277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32.0 1 22.0 2 15.5 3 27.0 4 21.0 5 12.0 6 19.0 7 36.5 8 48.5 9 43.5 10 38.0 11 29.0 12 25.0 13 28.0 14 23.5 15 16.5 16 21.0 17 30.5 18 46.0 19 59.0 20 78.0 21 102.0 22 120.0 23 144.5 24 175.5 25 234.5 26 316.5 27 386.5 28 452.5 29 596.0 30 760.5 31 849.0 32 983.0 33 1228.0 34 1572.5 35 2024.5 36 2588.0 37 3237.0 38 3961.0 39 4745.5 40 5588.0 41 6265.0 42 6682.5 43 6655.0 44 6176.5 45 6031.5 46 6179.5 47 7673.0 48 10734.0 49 10952.0 50 10164.5 51 8352.0 52 4073.0 53 1569.5 54 931.5 55 958.0 56 848.5 57 607.0 58 563.5 59 546.5 60 402.0 61 311.5 62 222.0 63 128.0 64 103.5 65 68.0 66 29.0 67 31.5 68 32.0 69 19.5 70 20.0 71 16.5 72 6.5 73 7.0 74 7.0 75 3.0 76 1.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02107827064499508 2 0.10461067653442004 3 0.0 4 0.0 5 0.0 6 0.0023420300716661202 7 0.02732368416943807 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 7.806766905553733E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.9033834527768665E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.806766905553733E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 128094.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.591909066779085 #Duplication Level Percentage of deduplicated Percentage of total 1 77.18845964287068 15.894577419707403 2 9.830534177503129 4.048589317220166 3 3.7381051673806724 2.3092416506627944 4 1.8690525836903362 1.5394944337751963 5 1.330704780680138 1.3700875919246802 6 0.9288395192781591 1.1475947351163989 7 0.5952155286802896 0.8579636829203553 8 0.5193918944535012 0.8556216528486893 9 0.4246123516700156 0.7869221040798164 >10 2.957121734844751 11.702343591425048 >50 0.31087690032983284 4.445953752712851 >100 0.22367972096902605 9.688978406482738 >500 0.04928536224741252 7.113526004340562 >1k 0.02274709026803655 9.019938482676784 >5k 0.0075823634226788485 11.834277952128906 >10k+ 0.0037911817113394242 17.38488922197761 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 22269 17.38488922197761 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 9744 7.606913672771558 RNA PCR Primer, Index 39 (95% over 23bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 5415 4.227364279357347 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 4523 3.5310006713819537 RNA PCR Primer, Index 39 (96% over 25bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1946 1.5191968398207567 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 1568 1.2241010507908257 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 1237 0.9656970662169969 RNA PCR Primer, Index 39 (96% over 28bp) TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 1218 0.9508642090964448 RNA PCR Primer, Index 39 (95% over 22bp) CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 1062 0.8290786453698065 RNA PCR Primer, Index 39 (96% over 30bp) CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC 911 0.7111964650959451 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.7026090214998361 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 872 0.6807500741642856 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 770 0.6011210517276375 Illumina PCR Primer Index 11 (96% over 25bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 729 0.5691133074148672 No Hit ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC 718 0.5605258638187581 Illumina PCR Primer Index 11 (96% over 26bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 672 0.5246147360532109 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA 631 0.4926069917404406 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 624 0.48714225490655305 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 615 0.4801161646915546 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 581 0.45357315721267194 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 564 0.44030165347323064 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 525 0.40985526254157106 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 482 0.37628616484769 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCT 478 0.37316345808546847 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 463 0.3614533077271379 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 457 0.3567692475838056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 421 0.3286648867238122 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 382 0.29821849579215265 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 367 0.28650834543382203 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 355 0.2771402251471575 No Hit ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT 341 0.26621075147938233 Illumina PCR Primer Index 11 (96% over 28bp) GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 338 0.2638687214077162 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 335 0.26152669133605005 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 326 0.2545006011210517 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 301 0.2349836838571674 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 266 0.2076599996877293 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 266 0.2076599996877293 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 265 0.20687932299717393 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 258 0.20141458616328634 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 243 0.18970443580495572 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 232 0.1811169922088466 No Hit CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCTCTCTA 230 0.1795556388277359 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 211 0.1647227817071838 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 208 0.16238075163551766 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 206 0.16081939825440691 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 204 0.15925804487329617 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT 203 0.1584773681827408 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 200 0.1561353381110747 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 192 0.1498899245866317 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 191 0.14910924789607632 No Hit CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 186 0.14520586444329947 No Hit CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 184 0.1436445110621887 RNA PCR Primer, Index 39 (95% over 21bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 181 0.1413024809905226 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 173 0.1350570674660796 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 157 0.1225662404171936 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 147 0.11475947351163988 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 146 0.11397879682108453 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 142 0.11085609005886302 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 132 0.1030493231533093 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 132 0.1030493231533093 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA 131 0.10226864646275392 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.806766905553734E-4 0.0 3 0.0 0.0 0.0 0.0015613533811107468 0.0 4 0.0 0.0 0.0 0.0023420300716661202 0.0 5 0.0 0.0 0.0 0.0023420300716661202 0.0 6 0.0 0.0 0.0 0.0031227067622214937 0.0 7 0.0 0.0 0.0 0.003903383452776867 0.0 8 0.0 0.0 0.0 0.0046840601433322405 0.0 9 0.0 0.0 0.0 0.009368120286664481 0.0 10-11 0.0 0.0 0.0 0.0117101503583306 0.0 12-13 0.0 0.0 0.0 0.014442518775274409 0.0 14-15 0.0 0.0 0.0 0.02263962402610583 0.0 16-17 0.0 0.0 0.0 0.02849469920527113 0.0 18-19 0.0 0.0 0.0 0.03395943603915874 0.0 20-21 0.0 0.0 0.0 0.04605992474276703 0.0 22-23 0.0 0.0 0.0 0.08743578934220182 0.0 24-25 0.0 0.0 0.0 0.22990928536855748 0.0 26-27 0.0 0.0 0.0 0.4762127812387778 0.0 28-29 0.0 0.0 0.0 0.7853607506987057 0.0 30-31 0.0 0.0 0.0 1.3341764641591332 0.0 32-33 0.0 0.0 0.0 2.064109169828407 0.0 34-35 0.0 0.0 0.0 3.390088528736709 0.0 36-37 0.0 0.0 0.0 4.709041797430013 0.0 38-39 0.0 0.0 0.0 6.188033787687167 0.0 40-41 0.0 0.0 0.0 8.375880212968601 0.0 42-43 0.0 0.0 0.0 9.580074008150266 0.0 44-45 0.0 0.0 0.0 10.425937202367013 0.0 46-47 0.0 0.0 0.0 11.31903133636236 0.0 48-49 0.0 0.0 0.0 12.173481974175216 0.0 50-51 0.0 0.0 0.0 12.988898777460303 0.0 52-53 0.0 0.0 0.0 13.871063437787875 0.0 54-55 0.0 0.0 0.0 14.536199978141052 0.0 56-57 0.0 0.0 0.0 14.998360578949834 0.0 58-59 0.0 0.0 0.0 15.340296969413087 0.0 60-61 0.0 0.0 0.0 15.64710290880135 0.0 62-63 0.0 0.0 0.0 15.946882757974613 0.0 64-65 0.0 0.0 0.0 16.222461629740657 0.0 66-67 0.0 0.0 0.0 16.537074336034472 0.0 68-69 0.0 0.0 0.0 16.96644651583993 0.0 70-71 0.0 0.0 0.0 17.387621590394556 0.0 72-73 0.0 0.0 0.0 17.68662076287726 0.0 74-75 0.0 0.0 0.0 18.01294361952941 0.0 76-77 0.0 0.0 0.0 18.288912829640733 0.0 78-79 0.0 0.0 0.0 18.61289365622121 0.0 80-81 0.0 0.0 0.0 18.960294783518354 0.0 82-83 0.0 0.0 0.0 19.292082377004387 0.0 84-85 0.0 0.0 0.0 19.61606320358487 0.0 86-87 0.0 0.0 0.0 19.893984105422582 0.0 88-89 0.0 0.0 0.0 20.258169781566664 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGATGA 20 0.0020700602 71.24824 9 TGGGACT 30 1.2969914E-4 63.33177 4 TTTTGTA 40 7.4564523E-6 59.44317 2 GGATACC 25 0.004988399 57.06544 1 CTTTACA 50 4.1787825E-7 57.06544 1 GTTATTA 50 4.1787825E-7 57.06544 1 GGGACTG 25 0.0050116084 56.998592 5 GCTCGGA 35 2.76286E-4 54.348038 2 GGCTCGG 35 2.76286E-4 54.348038 1 GATGTGT 280 0.0 49.195217 3 TGTGTAT 280 0.0 49.195217 5 ATGTGTA 290 0.0 47.498825 4 TCTCGGA 20 5.3809467E-4 47.498825 38-39 GTGTATA 295 0.0 46.693764 6 AGATGTG 300 0.0 45.969383 2 TGTATAA 290 0.0 45.860935 7 GTATAAG 290 0.0 45.860935 8 GAGATGT 305 0.0 45.21579 1 TATAAGA 285 0.0 44.99889 9 GTATATG 75 1.337994E-7 44.38423 1 >>END_MODULE