FastQCFastQC Report
Fri 10 Feb 2017
SRR2568670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671969
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44600.041.6418951
GGTATCA25050.039.440661
GTACATG149950.035.6364671
TACATGG147550.035.4752162
TCAACGC50450.035.2121934
CAACGCA51600.034.5194745
ACATGGG152950.033.7258573
ATCAACG54450.032.887143
AACGCAG56000.032.231336
TATCAAC60700.029.813912
CATGGGG118850.028.7350774
ACGCAGA66850.026.5026977
CGCAGAG73550.023.8947058
ATGGGGG64350.023.177355
GAGTACA95600.021.9610421
AGTACAT93950.021.4363922
CATGGGA47900.020.9232714
ATGGGGA51800.020.8151115
CGTATAC4000.020.186993
TATACTG15450.019.0610085
Produced by FastQC (version 0.11.5)