FastQCFastQC Report
Fri 10 Feb 2017
SRR2568670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671969
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40250.041.843141
GTACATG142750.036.694261
TACATGG141500.036.5362362
TCAACGC45600.035.6357424
ACATGGG143100.035.1957933
CAACGCA47450.034.246365
GGTATCA24750.033.447291
ATCAACG50750.032.113133
AACGCAG51500.031.5532026
CATGGGG105750.029.9239144
ACCGTAT4350.029.4812098
TATCAAC57850.028.6105752
TACCGTA3900.026.8014897
ACGCAGA63400.025.254667
ATGGGGG56200.023.841715
CGCAGAG68500.023.2287048
GAGTACA90300.021.0219021
AGTACAT85850.020.664232
CCGTATG4950.020.1504559
TATACCG2902.4338078E-919.66115
Produced by FastQC (version 0.11.5)