FastQCFastQC Report
Fri 10 Feb 2017
SRR2568669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876854
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.044.2597851
GTATCAA29400.040.3897171
TCAACGC35800.032.2396134
CAACGCA36350.031.7518065
ATCAACG36700.031.5784153
AACGCAG38450.030.2646966
TATCAAC39550.029.4229492
GTACATG61400.027.9265291
TACATGG64050.026.0288242
ACGCAGA44300.025.9464657
ACATGGG64950.024.9368613
CGCAGAG46200.024.8794048
TACCGTA1903.2912067E-722.49867
CATGGGG43200.022.2092654
GTACCGT2606.2573235E-1021.9217136
GTATAGA8100.021.1103611
GTATATA13200.019.4311261
GCAGAGT57350.019.379799
ACCGTAT2502.1752749E-718.9988168
AGAGTAC49850.018.91306510-11
Produced by FastQC (version 0.11.5)