##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568669_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1876854 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72991079753673 33.0 31.0 34.0 30.0 34.0 2 31.869594012107495 34.0 31.0 34.0 30.0 34.0 3 31.907887347657304 34.0 31.0 34.0 30.0 34.0 4 35.26494975101952 37.0 35.0 37.0 33.0 37.0 5 35.29393975237285 37.0 35.0 37.0 32.0 37.0 6 35.411179026178914 37.0 35.0 37.0 33.0 37.0 7 35.38483494187614 37.0 35.0 37.0 33.0 37.0 8 35.40521585589502 37.0 35.0 37.0 33.0 37.0 9 37.15878379458392 39.0 37.0 39.0 33.0 39.0 10-11 37.11413993842888 39.0 37.0 39.0 33.0 39.0 12-13 37.082239481600595 39.0 37.0 39.0 33.0 39.0 14-15 38.4375779895506 40.5 38.0 41.0 33.0 41.0 16-17 38.39537412073608 40.5 38.0 41.0 33.0 41.0 18-19 38.36711566269939 40.0 38.0 41.0 33.0 41.0 20-21 38.35542402339234 40.0 38.0 41.0 33.0 41.0 22-23 38.284698223729706 40.0 38.0 41.0 33.0 41.0 24-25 38.22174527160877 40.0 38.0 41.0 33.0 41.0 26-27 38.122101399469535 40.0 38.0 41.0 33.0 41.0 28-29 37.98403072375368 40.0 38.0 41.0 32.0 41.0 30-31 37.89198573783576 40.0 38.0 41.0 32.0 41.0 32-33 37.77862183206579 40.0 38.0 41.0 32.0 41.0 34-35 37.62389695735523 40.0 38.0 41.0 31.0 41.0 36-37 37.512891519532154 40.0 37.5 41.0 31.0 41.0 38-39 37.40123099612437 40.0 37.0 41.0 31.0 41.0 40-41 37.26013531153728 40.0 37.0 41.0 30.0 41.0 42-43 37.094055797627306 40.0 37.0 41.0 30.0 41.0 44-45 36.894202479255185 40.0 36.0 41.0 30.0 41.0 46-47 36.95272141573079 40.0 36.5 41.0 30.0 41.0 48-49 37.14681189906088 40.0 37.0 41.0 30.0 41.0 50-51 36.03162819270972 38.5 35.5 40.0 29.0 40.5 52-53 36.18122507131615 39.0 35.5 40.0 28.5 40.5 54-55 36.777369736804246 39.5 36.0 41.0 29.0 41.0 56-57 36.74058557564946 40.0 36.0 41.0 29.0 41.0 58-59 36.57394581571076 39.0 35.0 41.0 29.0 41.0 60-61 36.343331980004834 39.0 35.0 41.0 28.5 41.0 62-63 36.0565113216052 39.0 35.0 41.0 28.0 41.0 64-65 35.86536459415596 38.0 35.0 40.5 28.0 41.0 66-67 35.72563715664617 37.5 35.0 40.0 28.0 41.0 68-69 35.41723836803502 37.0 35.0 40.0 28.0 41.0 70-71 35.05515959152923 36.5 34.0 39.5 28.0 41.0 72-73 34.688720060271066 36.0 34.0 39.0 27.5 41.0 74-75 34.33761230228883 35.5 34.0 39.0 27.0 40.5 76-77 33.98498577939467 35.0 34.0 37.5 26.0 39.5 78-79 33.62725630230162 35.0 34.0 37.0 26.0 39.0 80-81 33.27554380894838 35.0 33.0 36.5 26.0 39.0 82-83 32.97533505536392 35.0 33.0 36.0 26.0 37.5 84-85 32.702400932624485 35.0 33.0 36.0 25.5 37.0 86-87 32.4805317835058 35.0 33.0 35.0 25.0 37.0 88-89 32.28031429189484 35.0 33.0 35.0 25.0 36.0 90-91 32.11876176836344 35.0 33.0 35.0 25.0 36.0 92-93 31.96732750656151 35.0 32.0 35.0 24.0 36.0 94-95 31.823796629892364 35.0 32.0 35.0 24.0 36.0 96-97 31.71987831765284 35.0 32.0 35.0 24.0 35.0 98-99 31.58012184218911 35.0 32.0 35.0 23.0 35.0 100-101 30.208199732104895 34.0 29.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02247797778934313 1101 2 0.12988319917960212 1101 3 0.15086974207410364 1101 4 0.12750672920350326 1101 5 -0.14012805409905127 1101 6 -0.05945674014341762 1101 7 0.031775183240249305 1101 8 0.06410554935802537 1101 9 0.08917249950648909 1101 10-11 -0.04879511719531848 1101 12-13 0.03076077856114523 1101 14-15 0.10539202191983321 1101 16-17 0.06684471188377472 1101 18-19 0.16349235961882158 1101 20-21 -0.03038609484169541 1101 22-23 -0.09970049538700465 1101 24-25 0.05441200328570517 1101 26-27 0.1707234701466689 1101 28-29 0.0690750977922221 1101 30-31 0.010342862695054578 1101 32-33 0.02560720920754278 1101 34-35 -0.179982640209019 1101 36-37 -0.15533416566097458 1101 38-39 -0.10293192877202273 1101 40-41 -0.06157369433892512 1101 42-43 -0.0722598167614592 1101 44-45 0.07303872853151461 1101 46-47 0.12390654949263791 1101 48-49 0.09482169257233153 1101 50-51 -0.03953470106738166 1101 52-53 -0.02796810756869661 1101 54-55 0.040957404688597876 1101 56-57 -0.06487671151463559 1101 58-59 -0.03892558907515564 1101 60-61 -0.10929239487728637 1101 62-63 0.08149272265752217 1101 64-65 -0.07598842410683204 1101 66-67 0.001674152192812528 1101 68-69 0.04010558155559352 1101 70-71 0.1300407151773726 1101 72-73 0.1346444914009126 1101 74-75 0.04650866945362253 1101 76-77 0.034719942221443034 1101 78-79 -0.1382327752400343 1101 80-81 -0.12773620330591484 1101 82-83 -0.056183391870987975 1101 84-85 -0.22571886518613837 1101 86-87 -0.10290691109084804 1101 88-89 -0.10219656653216447 1101 90-91 -0.1476873111892587 1101 92-93 -0.1145562078346849 1101 94-95 -0.18872705400487533 1101 96-97 -0.22352445008742095 1101 98-99 -0.17359842334240128 1101 100-101 -0.38541710056539813 1102 1 0.39958765194760204 1102 2 0.3214538739781858 1102 3 0.4499904928074123 1102 4 0.32467185480844307 1102 5 0.33006965045530023 1102 6 0.35378878254899604 1102 7 0.35475716625477816 1102 8 0.49405932240415495 1102 9 0.5406041571168245 1102 10-11 0.5748274537834774 1102 12-13 0.45947161242590084 1102 14-15 0.5554705362311907 1102 16-17 0.568005528589353 1102 18-19 0.4843407909162707 1102 20-21 0.3874282456691063 1102 22-23 0.36597802401822577 1102 24-25 0.33352899238513345 1102 26-27 0.5230290545729872 1102 28-29 0.637514140547836 1102 30-31 0.519141396180288 1102 32-33 0.3656314835011898 1102 34-35 0.35146422720220016 1102 36-37 0.17675933053281767 1102 38-39 0.3139771053207241 1102 40-41 0.25029383606680256 1102 42-43 0.2239868105843783 1102 44-45 0.3957896910624399 1102 46-47 0.3210013077276628 1102 48-49 0.41790179806788075 1102 50-51 0.3933258484381028 1102 52-53 0.15635880976254413 1102 54-55 0.19089035160547496 1102 56-57 0.34446529969908113 1102 58-59 0.23961588963226887 1102 60-61 0.11343649417704427 1102 62-63 0.299526753035984 1102 64-65 0.3454806114748905 1102 66-67 0.36846806788062025 1102 68-69 0.37371107824309746 1102 70-71 0.1994627786621237 1102 72-73 0.1753525863260066 1102 74-75 0.06326214431189925 1102 76-77 0.0281881849865826 1102 78-79 0.2219870974848206 1102 80-81 0.08515235600463456 1102 82-83 0.048374030025314596 1102 84-85 0.20110102928566675 1102 86-87 -0.04984926111283983 1102 88-89 -0.041938914203136335 1102 90-91 0.1324636374314565 1102 92-93 0.19346087233415687 1102 94-95 -0.0018811154429378973 1102 96-97 0.1984489121895976 1102 98-99 0.238560405117628 1102 100-101 -0.11631529840970956 1103 1 0.12516277733865167 1103 2 0.20972917182550788 1103 3 0.3207217352827847 1103 4 0.18031712481174367 1103 5 0.20151860851262882 1103 6 0.2245423374258877 1103 7 0.26587385546626763 1103 8 0.3017803430105843 1103 9 0.3102909002964367 1103 10-11 0.40153214450105423 1103 12-13 0.3360091925529929 1103 14-15 0.4250103894341848 1103 16-17 0.34764546220995385 1103 18-19 0.3267966425759852 1103 20-21 0.4484257029850198 1103 22-23 0.3931605720015128 1103 24-25 0.362404493833111 1103 26-27 0.193988534322159 1103 28-29 0.1805143218360925 1103 30-31 0.29990066861006426 1103 32-33 0.231560911234574 1103 34-35 0.21837679512719887 1103 36-37 0.22077333156477152 1103 38-39 0.2522586785309713 1103 40-41 0.17070457019129748 1103 42-43 0.17524072106516542 1103 44-45 0.21715939361023828 1103 46-47 0.3259225867860849 1103 48-49 0.2128954529787066 1103 50-51 0.18407735801151404 1103 52-53 0.11906409547749064 1103 54-55 0.24885006842391277 1103 56-57 0.17298705661476532 1103 58-59 0.21205263629818916 1103 60-61 0.3351613357822387 1103 62-63 0.2297033463971161 1103 64-65 0.19662993257359318 1103 66-67 0.1824003032578645 1103 68-69 0.21361634350501646 1103 70-71 0.12125920588883332 1103 72-73 0.0881255065080353 1103 74-75 0.010093550876391078 1103 76-77 0.05582023489198917 1103 78-79 0.09718500383780793 1103 80-81 0.19064957392707527 1103 82-83 0.1823348996511598 1103 84-85 0.15564927288131258 1103 86-87 0.06484484980768457 1103 88-89 -0.03545390767211387 1103 90-91 0.011256629478012314 1103 92-93 -0.1334873954332565 1103 94-95 -0.2273702451198254 1103 96-97 0.017951810477583763 1103 98-99 -0.06367548345052043 1103 100-101 -0.3379783160690444 1104 1 0.19806469537237348 1104 2 0.2876501125858013 1104 3 0.3067145289748616 1104 4 0.30380417228318635 1104 5 0.31495184946862764 1104 6 0.23853991197351831 1104 7 0.25500572739887417 1104 8 0.33851324991962173 1104 9 0.27368495750033617 1104 10-11 0.19652450476392147 1104 12-13 0.18977630534527634 1104 14-15 0.3736052469498006 1104 16-17 0.3392710844435598 1104 18-19 0.3381358512830914 1104 20-21 0.3106823368599194 1104 22-23 0.2640573326654092 1104 24-25 0.33372943002270006 1104 26-27 0.32922063785481726 1104 28-29 0.472632477266437 1104 30-31 0.3942428850532238 1104 32-33 0.43663028179903307 1104 34-35 0.3119235157317206 1104 36-37 0.37343760780365187 1104 38-39 0.17104936724560815 1104 40-41 0.22131027657145808 1104 42-43 0.33448567861974254 1104 44-45 0.34845823648557683 1104 46-47 0.2592900690877258 1104 48-49 0.3613502121628045 1104 50-51 0.39429709348835473 1104 52-53 0.32849271318854534 1104 54-55 0.4863885407391706 1104 56-57 0.36712306582622745 1104 58-59 0.363627210202246 1104 60-61 0.38946914057272153 1104 62-63 0.3803077393554162 1104 64-65 0.27704593705674085 1104 66-67 0.22908632100657655 1104 68-69 0.29604057000899076 1104 70-71 0.2645495903274053 1104 72-73 0.2924160179107318 1104 74-75 0.22556259571224047 1104 76-77 0.2875332434598761 1104 78-79 0.3729134057098733 1104 80-81 0.1934103866725181 1104 82-83 0.1480926783921106 1104 84-85 0.06322903042363492 1104 86-87 0.09187769848835359 1104 88-89 0.14890542703593468 1104 90-91 0.22352836770429008 1104 92-93 0.1795960375012342 1104 94-95 0.07833919969675307 1104 96-97 0.16814772923806487 1104 98-99 0.1974910102822669 1104 100-101 -0.021351270961758928 1105 1 0.24249806004749175 1105 2 0.05880074881102004 1105 3 0.27815718691555347 1105 4 0.10575384090429907 1105 5 0.23577032162514655 1105 6 0.19220608332215505 1105 7 0.2741889957258081 1105 8 0.28824304967896097 1105 9 0.21072983907221499 1105 10-11 0.2542629166447412 1105 12-13 0.3664536962689908 1105 14-15 0.45523064910943845 1105 16-17 0.43931682120786775 1105 18-19 0.32333748890789593 1105 20-21 0.4035490701360729 1105 22-23 0.45513902349927093 1105 24-25 0.406155461111922 1105 26-27 0.5317985163101895 1105 28-29 0.6103837268484895 1105 30-31 0.5593424622989573 1105 32-33 0.3128969578878653 1105 34-35 0.5505945061256909 1105 36-37 0.4735571188194072 1105 38-39 0.41977753840972554 1105 40-41 0.5492746110557363 1105 42-43 0.5769539982222369 1105 44-45 0.4506512783468253 1105 46-47 0.43981386300139746 1105 48-49 0.6071382095010449 1105 50-51 0.473124962946855 1105 52-53 0.5263200860203838 1105 54-55 0.5792125798254588 1105 56-57 0.4357653447864678 1105 58-59 0.5421041366916839 1105 60-61 0.5702479545706538 1105 62-63 0.5850952466101589 1105 64-65 0.6285015635620752 1105 66-67 0.5247205211137498 1105 68-69 0.48192861897285155 1105 70-71 0.3976636947210821 1105 72-73 0.3944677272814019 1105 74-75 0.4044041403978369 1105 76-77 0.37219729416683833 1105 78-79 0.39778743331289235 1105 80-81 0.25528915349419634 1105 82-83 0.2589586932281023 1105 84-85 0.3222275261486516 1105 86-87 0.4125159717193938 1105 88-89 0.39142936359073843 1105 90-91 0.35757421962840397 1105 92-93 0.4078607689596865 1105 94-95 0.5086673528017194 1105 96-97 0.35341908434837777 1105 98-99 0.38317802032145565 1105 100-101 0.2710836457534924 1106 1 0.29627153190445554 1106 2 0.13155078134595044 1106 3 0.19702667224520454 1106 4 0.19228837495902695 1106 5 0.26105672563293325 1106 6 0.2731343016063974 1106 7 0.2144658196467759 1106 8 0.32562858149297114 1106 9 0.43201824800469524 1106 10-11 0.23298024159073094 1106 12-13 0.2676222478139749 1106 14-15 0.2924841728676668 1106 16-17 0.23596288886248118 1106 18-19 0.3119209503407987 1106 20-21 0.2972297562429418 1106 22-23 0.2802879432752192 1106 24-25 0.2012285777261269 1106 26-27 0.1356934948500168 1106 28-29 0.15417992805737413 1106 30-31 0.18320454512708295 1106 32-33 0.2106995939257459 1106 34-35 0.3496893908618546 1106 36-37 0.22026078296102014 1106 38-39 0.32045709964561553 1106 40-41 0.27840458197402285 1106 42-43 0.23731801575115696 1106 44-45 0.3708002561058166 1106 46-47 0.35096677201054405 1106 48-49 0.2844931906952084 1106 50-51 0.3854736111044801 1106 52-53 0.39293589941292595 1106 54-55 0.40126552736111165 1106 56-57 0.46924604094373734 1106 58-59 0.44733601087106223 1106 60-61 0.5048031408959375 1106 62-63 0.4451976317747466 1106 64-65 0.42357113676823843 1106 66-67 0.3763970040944429 1106 68-69 0.41636171878485584 1106 70-71 0.4257484246940635 1106 72-73 0.33419596721264355 1106 74-75 0.41388758881677035 1106 76-77 0.4344404776161497 1106 78-79 0.4913230973926588 1106 80-81 0.5471889871472939 1106 82-83 0.42484108558258527 1106 84-85 0.3450448190106812 1106 86-87 0.4425048003202363 1106 88-89 0.5670145735344434 1106 90-91 0.4674131040862477 1106 92-93 0.5032982767712895 1106 94-95 0.47945195353694103 1106 96-97 0.5110353500086298 1106 98-99 0.4640257066048541 1106 100-101 0.4815044222366005 1107 1 0.13649829340874575 1107 2 0.08071471426011456 1107 3 0.1793760412828611 1107 4 0.14702671045963456 1107 5 0.15915880029916707 1107 6 0.15160214196058064 1107 7 0.16723867906924994 1107 8 0.20351794862193628 1107 9 0.20233372117713344 1107 10-11 0.19319114706393492 1107 12-13 0.12301087252454579 1107 14-15 0.16683432351062777 1107 16-17 0.18971955263411644 1107 18-19 0.2291007787395145 1107 20-21 0.18876096154122735 1107 22-23 0.15613865879596034 1107 24-25 0.14093887193707388 1107 26-27 0.1947488134181583 1107 28-29 0.22252587317332484 1107 30-31 0.20202331879583824 1107 32-33 0.07080970643745843 1107 34-35 0.042456796108197636 1107 36-37 0.022166359069700547 1107 38-39 -0.03339657168669419 1107 40-41 -0.10546558517541627 1107 42-43 0.14412691218952034 1107 44-45 0.17259600227496463 1107 46-47 0.1920165916175378 1107 48-49 0.09583454481306575 1107 50-51 0.0033516593518143623 1107 52-53 0.0855836113513817 1107 54-55 0.14166420471007513 1107 56-57 0.34549928852441525 1107 58-59 0.2460837096405939 1107 60-61 0.053962302072442014 1107 62-63 0.09449384045589682 1107 64-65 0.16394528898506877 1107 66-67 0.1144521984374478 1107 68-69 0.17125408277833998 1107 70-71 0.23869657483265883 1107 72-73 0.15645620338966637 1107 74-75 0.19486589633740437 1107 76-77 0.20714888392508612 1107 78-79 0.05034322772773692 1107 80-81 0.06295824724005428 1107 82-83 -0.09773229921087534 1107 84-85 0.04026833626651438 1107 86-87 0.1759056843979323 1107 88-89 0.06975645664872587 1107 90-91 -4.259172117002663E-4 1107 92-93 -0.016970945200878162 1107 94-95 0.058416214554728896 1107 96-97 0.07449572885794353 1107 98-99 0.0908414327593654 1107 100-101 0.15333242961759908 1108 1 0.05976415372968091 1108 2 0.08711936659957331 1108 3 0.21920977650812645 1108 4 0.17990179091808045 1108 5 0.14772649587322917 1108 6 -0.01983438953589456 1108 7 0.10779389838055664 1108 8 0.14914885491226215 1108 9 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-0.7420143303198756 2316 50-51 -0.9796374947503921 2316 52-53 -0.870349672266137 2316 54-55 -0.8444050871759714 2316 56-57 -1.1188314823884653 2316 58-59 -1.2678105526682728 2316 60-61 -0.9992952987469366 2316 62-63 -0.8643597801077618 2316 64-65 -0.9789334810531116 2316 66-67 -0.9101627376141863 2316 68-69 -0.5821433204113262 2316 70-71 -0.6220982053804462 2316 72-73 -0.683605586267575 2316 74-75 -0.48307093467180806 2316 76-77 -0.6352286161169687 2316 78-79 -0.46959449667095043 2316 80-81 -0.3634240300059943 2316 82-83 -0.44084338789379984 2316 84-85 -0.4568954324914003 2316 86-87 -0.4398639482096627 2316 88-89 -0.42246189671111267 2316 90-91 -0.3703816776113733 2316 92-93 -0.38875179937841153 2316 94-95 -0.3596638951938722 2316 96-97 -0.32555772498951896 2316 98-99 -0.23990985545966836 2316 100-101 -0.07657051239992896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 49.0 7 513.0 8 829.0 9 674.0 10 810.0 11 1529.0 12 2879.0 13 4103.0 14 5099.0 15 5741.0 16 6368.0 17 6878.0 18 7720.0 19 8584.0 20 9959.0 21 11012.0 22 12331.0 23 13139.0 24 13676.0 25 14419.0 26 16091.0 27 17893.0 28 20823.0 29 24381.0 30 29032.0 31 34866.0 32 43887.0 33 57810.0 34 80650.0 35 122227.0 36 216470.0 37 411043.0 38 526946.0 39 148370.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90543201127484 19.071957031988894 13.076896435051113 23.94571452168515 2 14.787010019672747 20.056449911227535 41.39205459773178 23.764485471367948 3 17.903805885455796 26.78900060207904 29.496704549692833 25.810488962772332 4 11.83384252091008 17.030805937194636 39.46599774302207 31.669353798873217 5 13.074377408696645 38.51245182606713 35.419676011153065 12.993494754083162 6 30.69303554320802 37.88112265939455 18.635274233545296 12.790567563852132 7 27.263887090577864 32.2491782384651 23.37367411194073 17.113260559016304 8 25.63748161725527 36.18709904303161 20.556650823760098 17.618768515953025 9 26.31817607794094 15.700619650525416 20.152629371829214 37.82857489970443 10-11 24.506772454068802 26.387900166247498 29.184161728973955 19.92116565070975 12-13 25.341313976889275 23.607316957508854 29.245957594638934 21.805411470962934 14-15 22.653227155655156 25.119694978938163 26.14707910151775 26.079998763888934 16-17 22.808833292307234 28.13186321365434 26.577346985966944 22.481956508071484 18-19 22.680602424114227 27.587919229581686 27.903234700899194 21.828243645404896 20-21 23.548307776573917 26.763027446997885 27.496762502627426 22.191902273800768 22-23 23.31670886055716 26.658022757794104 27.19282798878867 22.832440392860065 24-25 22.823070189132842 26.8912618038191 27.293083173800063 22.992584833247996 26-27 22.929748119454437 27.137134963694514 27.077991189708982 22.855125727142063 28-29 23.07423027954741 26.923998089302465 27.232075421934603 22.76969620921552 30-31 23.124001710497044 26.89615391276199 27.163971923336693 22.815872453404275 32-33 23.053350393865454 26.893808310908753 27.153179548780358 22.899661746445435 34-35 23.254339442492597 27.06084223919388 27.105011897569014 22.5798064207445 36-37 23.163709057816963 26.783702941198413 27.164686224927458 22.887901776057166 38-39 23.182269251313038 26.9789714428811 27.047596974189503 22.791162331616363 40-41 23.15803466865297 26.970265135167683 27.022107207060326 22.849592989119024 42-43 23.043075411541054 26.83760219261229 27.09217794941306 23.027144446433596 44-45 23.13595661630171 26.940608492812895 26.998658378950935 22.92477651193446 46-47 23.16134657820766 26.90470995467555 26.87369974928089 23.060243717835903 48-49 23.212092100604462 26.93338192680023 27.00219603041461 22.852329942180702 50-51 23.188961788202672 27.03816810569326 26.8607640867556 22.912106019348467 52-53 23.146286285454277 27.004071707229226 26.85387355649401 22.995768450822492 54-55 23.0753697410667 26.913894208073724 26.99988917624919 23.010846874610387 56-57 23.083201996532495 27.092304462680634 26.809650617469444 23.014842923317424 58-59 23.149829448641185 26.854832608183692 26.9438379330518 23.051500010123323 60-61 23.31803645888279 27.01153099814903 26.920207965030844 22.750224577937335 62-63 23.05701227999835 27.06578770348446 26.925631349627633 22.95156866688956 64-65 23.101365220900973 26.925848617529013 26.974708313741058 22.998077847828956 66-67 23.02500967074703 26.99859441153371 26.925171329405355 23.0512245883139 68-69 22.994519620929346 26.909513799525232 26.98387324525296 23.112093334292467 70-71 23.213773360069755 26.716281964290516 26.874926239070398 23.19501843656933 72-73 23.034317000683057 26.95534655332807 26.804748797722144 23.205587648266725 74-75 23.02254197715965 26.932089549853107 26.89990803759909 23.145460435388156 76-77 23.15728873956099 26.826460662363722 26.887600207581407 23.12865039049388 78-79 23.089435832515477 26.766919536628848 26.92164654256538 23.2219980882903 80-81 23.112044372362714 26.971179119815986 26.720695991923577 23.196080515897723 82-83 23.04325572832198 26.887643919102366 26.958196351575985 23.11090400099967 84-85 22.99711645093783 26.918444748674 26.815700660855512 23.26873813953265 86-87 23.09035613930098 26.879188890623762 26.873780002675137 23.156674967400125 88-89 22.96692392914029 26.868279233253737 26.84427245060491 23.32052438700106 90-91 23.000289890289316 26.913648930070362 26.94945890698572 23.136602272654606 92-93 23.206318469491226 26.733281574629782 26.998380631090285 23.062019324788707 94-95 23.070431460076545 26.694528759963642 27.034437771149783 23.20060200881003 96-97 22.948432249586762 26.594393804239285 27.137759120299172 23.31941482587478 98-99 22.987116554772825 26.621994393451192 27.04233900889156 23.348550042884423 100-101 23.06835373694344 26.52664708104833 26.973984664386236 23.431014517621996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13.0 1 12.5 2 18.5 3 35.0 4 36.5 5 39.0 6 62.5 7 98.5 8 168.0 9 219.5 10 214.5 11 206.5 12 220.0 13 257.5 14 304.0 15 352.5 16 390.0 17 448.0 18 511.0 19 554.5 20 640.5 21 780.5 22 975.0 23 1226.0 24 1631.5 25 2269.5 26 3280.5 27 5030.0 28 7692.0 29 11539.5 30 16269.0 31 21510.0 32 27012.5 33 32621.0 34 37821.5 35 42470.5 36 47507.5 37 51309.5 38 54795.0 39 59312.5 40 63921.0 41 67684.5 42 68558.5 43 70433.0 44 72965.0 45 73759.5 46 75102.0 47 76095.5 48 76094.5 49 75412.5 50 73432.0 51 70207.0 52 67748.0 53 66713.0 54 65209.5 55 61242.0 56 55313.0 57 49109.5 58 43657.5 59 38173.5 60 32367.5 61 26698.0 62 20806.0 63 15462.0 64 11219.5 65 8010.5 66 5798.0 67 4330.5 68 3187.5 69 2320.0 70 1718.0 71 1195.5 72 836.0 73 599.5 74 456.0 75 350.0 76 232.0 77 140.5 78 89.0 79 66.0 80 47.5 81 37.0 82 36.0 83 37.0 84 27.5 85 16.0 86 14.0 87 15.0 88 11.0 89 8.0 90 7.0 91 5.0 92 6.0 93 7.0 94 6.5 95 4.5 96 2.5 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005807590787562592 2 0.007778974816368241 3 0.0012787355862523139 4 4.262451954174379E-4 5 0.003250119615057964 6 0.0051149423450092555 7 0.004955100396727715 8 0.005008381046154895 9 0.00804537806350414 10-11 0.007139607023242085 12-13 0.0033566809139123233 14-15 0.0 16-17 0.0 18-19 1.8648227299512907E-4 20-21 9.85692014402825E-4 22-23 1.3320162356794934E-4 24-25 0.0013320162356794934 26-27 0.0038095664340433515 28-29 0.002797234094926936 30-31 0.011002454106712616 32-33 3.99604870703848E-4 34-35 0.0 36-37 0.0 38-39 7.992097414076962E-5 40-41 0.0 42-43 3.1968389656307847E-4 44-45 0.0010656129885435947 46-47 0.002797234094926936 48-49 0.003116917991490015 50-51 0.003436601888053093 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0011455339626843643 64-65 0.0027439534454997564 66-67 0.003196838965630784 68-69 0.008471623258921578 70-71 7.192887672669264E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014945222164323917 82-83 0.012547592940100827 84-85 0.01848838535123137 86-87 0.01667684327070726 88-89 0.016037475477581102 90-91 0.015025143138464687 92-93 0.010496287937154408 94-95 0.02501526490606089 96-97 0.011508620276270823 98-99 0.015904273854013152 100-101 0.01635715937414418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1876854.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.026873985938856 #Duplication Level Percentage of deduplicated Percentage of total 1 67.59729973359137 34.49278895295704 2 15.881732776235461 16.207903541026432 3 6.5666333371674 10.052243154225183 4 3.361280046023036 6.860624533594728 5 1.8923140419758844 4.827943508086303 6 1.2230415800917878 3.7444793152144316 7 0.7867304022804984 2.810107516865178 8 0.5385926734183086 2.1986160381012767 9 0.40138748971321914 1.8433393971415883 >10 1.6854899612339194 13.91830716827845 >50 0.049498993036579494 1.6748668614667874 >100 0.015683338136429966 1.2275350932391895 >500 2.1041806407238626E-4 0.07660832404387927 >1k 1.0520903203619313E-4 0.06463659575951691 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.3280649427179735E-5 0.0 5 0.0 0.0 0.0 5.3280649427179735E-5 0.0 6 0.0 0.0 0.0 5.3280649427179735E-5 5.3280649427179735E-5 7 0.0 0.0 0.0 5.3280649427179735E-5 5.3280649427179735E-5 8 0.0 0.0 0.0 5.3280649427179735E-5 5.3280649427179735E-5 9 0.0 0.0 0.0 1.0656129885435947E-4 1.0656129885435947E-4 10-11 0.0 0.0 0.0 1.3320162356794934E-4 1.0656129885435947E-4 12-13 0.0 0.0 0.0 1.8648227299512907E-4 1.0656129885435947E-4 14-15 0.0 0.0 0.0 2.1312259770871894E-4 1.3320162356794934E-4 16-17 0.0 0.0 0.0 2.1312259770871894E-4 1.598419482815392E-4 18-19 0.0 0.0 0.0 2.1312259770871894E-4 1.598419482815392E-4 20-21 0.0 0.0 0.0 4.5288552013102775E-4 1.598419482815392E-4 22-23 0.0 0.0 0.0 6.660081178397467E-4 1.8648227299512907E-4 24-25 0.0 0.0 0.0 7.99209741407696E-4 2.1312259770871894E-4 26-27 0.0 0.0 0.0 0.0011455339626843643 2.1312259770871894E-4 28-29 0.0 0.0 0.0 0.0017049807816697515 2.1312259770871894E-4 30-31 0.0 0.0 0.0 0.0027173131207861665 2.1312259770871894E-4 32-33 0.0 0.0 0.0 0.0057276698134218216 2.1312259770871894E-4 34-35 0.0 0.0 0.0 0.009936841118169022 3.7296454599025815E-4 36-37 0.0 0.0 0.0 0.017609254635682903 3.7296454599025815E-4 38-39 0.0 0.0 0.0 0.03332704621670093 3.7296454599025815E-4 40-41 0.0 0.0 0.0 0.05266792195876717 3.7296454599025815E-4 42-43 0.0 0.0 0.0 0.07344737523536726 3.99604870703848E-4 44-45 0.0 0.0 0.0 0.09774335137416124 4.262451954174379E-4 46-47 0.0 0.0 0.0 0.12496976323145007 4.262451954174379E-4 48-49 0.0 0.0 0.0 0.15653854801705408 4.795258448446176E-4 50-51 0.0 0.0 0.0 0.19202346053555577 4.795258448446176E-4 52-53 0.0 0.0 0.0 0.23081177331854263 5.328064942717974E-4 54-55 0.0 0.0 0.0 0.27271700409301947 5.860871436989771E-4 56-57 0.0 0.0 0.0 0.31971053688779205 6.12727468412567E-4 58-59 0.0 0.0 0.0 0.37376375573166587 6.393677931261568E-4 60-61 0.0 0.0 0.0 0.4365283607568836 6.393677931261568E-4 62-63 0.0 0.0 0.0 0.5018770772793195 6.393677931261568E-4 64-65 0.0 0.0 0.0 0.5704226327673863 6.393677931261568E-4 66-67 0.0 0.0 0.0 0.6489316696983356 6.393677931261568E-4 68-69 0.0 0.0 0.0 0.7403612641153761 6.393677931261568E-4 70-71 0.0 0.0 0.0 0.8407420076361827 6.926484425533366E-4 72-73 0.0 0.0 0.0 0.9488484453239303 7.459290919805163E-4 74-75 0.0 0.0 0.0 1.0649203401010414 7.459290919805163E-4 76-77 0.0 0.0 0.0 1.1899966646313458 7.459290919805163E-4 78-79 0.0 0.0 0.0 1.327886985348887 7.459290919805163E-4 80-81 0.0 0.0 0.0 1.4864768383688873 7.725694166941062E-4 82-83 0.0 0.0 0.0 1.6593991860847992 7.99209741407696E-4 84-85 0.0 0.0 0.0 1.8431375056344286 7.99209741407696E-4 86-87 0.0 0.0 0.0 2.038810690655746 7.99209741407696E-4 88-89 0.0 0.0 0.0 2.250254947907509 7.99209741407696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1760 0.0 44.259785 1 GTATCAA 2940 0.0 40.389717 1 TCAACGC 3580 0.0 32.239613 4 CAACGCA 3635 0.0 31.751806 5 ATCAACG 3670 0.0 31.578415 3 AACGCAG 3845 0.0 30.264696 6 TATCAAC 3955 0.0 29.422949 2 GTACATG 6140 0.0 27.926529 1 TACATGG 6405 0.0 26.028824 2 ACGCAGA 4430 0.0 25.946465 7 ACATGGG 6495 0.0 24.936861 3 CGCAGAG 4620 0.0 24.879404 8 TACCGTA 190 3.2912067E-7 22.4986 7 CATGGGG 4320 0.0 22.209265 4 GTACCGT 260 6.2573235E-10 21.921713 6 GTATAGA 810 0.0 21.110361 1 GTATATA 1320 0.0 19.431126 1 GCAGAGT 5735 0.0 19.37979 9 ACCGTAT 250 2.1752749E-7 18.998816 8 AGAGTAC 4985 0.0 18.913065 10-11 >>END_MODULE