FastQCFastQC Report
Fri 10 Feb 2017
SRR2568669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876854
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.041.9433821
GTATCAA36300.039.7235571
TCAACGC40850.034.7801134
ATCAACG41100.034.5685543
CAACGCA41200.034.484655
AACGCAG43050.033.1131066
TATCAAC44400.032.144842
ACGCAGA49950.027.9681477
CGCAGAG50550.027.4364818
GTACATG58800.026.5465831
TACATGG61150.024.584632
ACATGGG62300.023.1866173
GCAGAGT63450.021.5589479
AGAGTAC53750.020.28013810-11
ACCCGTG2601.48538675E-820.1034777
TATACAG12950.019.8141675
TTACGTA1456.1329565E-419.6623364
ATAGTCG1456.1505905E-419.6539558
CATGGGG39500.019.127224
CGTATAC1559.506513E-418.4217821
Produced by FastQC (version 0.11.5)