##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568669_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1876854 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.689129788465166 34.0 31.0 34.0 31.0 34.0 2 32.78964320080305 34.0 31.0 34.0 31.0 34.0 3 32.860551753093205 34.0 31.0 34.0 31.0 34.0 4 36.24972906789766 37.0 37.0 37.0 35.0 37.0 5 36.30013309506227 37.0 37.0 37.0 35.0 37.0 6 36.31470055742216 37.0 37.0 37.0 35.0 37.0 7 36.24211206625555 37.0 37.0 37.0 35.0 37.0 8 36.23779260400649 37.0 37.0 37.0 35.0 37.0 9 38.06425593040269 39.0 39.0 39.0 35.0 39.0 10-11 37.989592690747386 39.0 38.5 39.0 35.0 39.0 12-13 37.983749135521464 39.0 39.0 39.0 35.0 39.0 14-15 39.45000703304572 41.0 39.0 41.0 36.0 41.0 16-17 39.43039788923379 41.0 39.0 41.0 36.0 41.0 18-19 39.464017979022344 41.0 39.5 41.0 36.5 41.0 20-21 39.43359712582865 41.0 39.0 41.0 36.0 41.0 22-23 39.404172354375994 41.0 39.0 41.0 36.0 41.0 24-25 39.37641313602443 41.0 39.0 41.0 36.0 41.0 26-27 39.28063237737192 41.0 39.0 41.0 36.0 41.0 28-29 39.17795736908678 41.0 39.0 41.0 36.0 41.0 30-31 39.13254227553129 41.0 39.0 41.0 36.0 41.0 32-33 39.01370031979046 40.5 39.0 41.0 35.0 41.0 34-35 38.919133827138396 40.0 38.5 41.0 35.0 41.0 36-37 38.8418656965326 40.0 38.0 41.0 35.0 41.0 38-39 38.709425453444965 40.0 38.0 41.0 35.0 41.0 40-41 38.63153553766036 40.0 38.0 41.0 35.0 41.0 42-43 38.51212134774468 40.0 38.0 41.0 34.0 41.0 44-45 38.402147689697756 40.0 38.0 41.0 34.0 41.0 46-47 38.22965238638701 40.0 38.0 41.0 33.5 41.0 48-49 38.096389223668965 40.0 38.0 41.0 33.0 41.0 50-51 38.09193629339309 40.0 38.0 41.0 33.5 41.0 52-53 38.173441034838085 40.0 38.0 41.0 34.0 41.0 54-55 38.03627426533976 40.0 37.0 41.0 33.0 41.0 56-57 37.877280811400354 40.0 37.0 41.0 33.0 41.0 58-59 37.66208772765489 40.0 36.5 41.0 33.0 41.0 60-61 37.55706943640794 39.5 36.0 41.0 33.0 41.0 62-63 37.34578928355642 39.0 36.0 41.0 33.0 41.0 64-65 37.09232151248845 39.0 35.0 41.0 32.0 41.0 66-67 36.82514116708066 38.5 35.0 41.0 32.0 41.0 68-69 36.50661399341664 37.5 35.0 40.0 32.0 41.0 70-71 36.1397354296072 37.0 35.0 39.5 31.0 41.0 72-73 35.739854565139325 36.5 35.0 39.0 31.0 41.0 74-75 35.36446601600338 36.0 35.0 39.0 31.0 40.5 76-77 33.59663165062386 34.5 32.5 36.5 28.0 39.0 78-79 34.545242464251345 35.0 34.0 37.0 30.0 39.0 80-81 34.45103401756343 35.0 34.0 37.0 31.0 39.0 82-83 34.23168664158214 35.0 34.0 36.0 31.0 38.0 84-85 33.95481800928575 35.0 34.0 36.0 30.0 37.0 86-87 33.75286410130996 35.0 34.0 35.5 30.0 37.0 88-89 33.58446101827846 35.0 34.0 35.0 30.0 36.5 90-91 33.464734870160385 35.0 34.0 35.0 30.0 36.0 92-93 33.36244854421281 35.0 34.0 35.0 30.0 36.0 94-95 33.26930011604526 35.0 34.0 35.0 29.5 36.0 96-97 33.18208848424012 35.0 34.0 35.0 29.0 35.5 98-99 33.10401847986044 35.0 34.0 35.0 29.0 35.0 100-101 31.983647369481055 34.5 32.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2393646256713069 1101 2 0.08699645792825805 1101 3 0.0760672214186755 1101 4 0.11820980215603782 1101 5 0.09600548219167138 1101 6 0.0804373221389838 1101 7 0.22390438803018498 1101 8 0.15720208626232335 1101 9 0.10690423933463933 1101 10-11 0.18994311250308016 1101 12-13 0.21751157898172124 1101 14-15 0.3210389302779717 1101 16-17 0.30157986802837655 1101 18-19 0.23939401298530782 1101 20-21 0.26844021506911986 1101 22-23 0.2364995320953298 1101 24-25 0.25877743555023613 1101 26-27 0.19023769073154995 1101 28-29 0.2592650934511056 1101 30-31 0.2353967125683596 1101 32-33 0.18540792846808785 1101 34-35 0.29073743048289913 1101 36-37 0.142420094751202 1101 38-39 0.259022220399288 1101 40-41 0.2239052131339676 1101 42-43 0.15953712681535848 1101 44-45 0.11466059786733496 1101 46-47 0.19202774276307366 1101 48-49 0.14762939821957843 1101 50-51 0.05751290193632741 1101 52-53 0.13553344946700463 1101 54-55 0.1542812188698619 1101 56-57 0.09967812821244593 1101 58-59 0.13975525984997716 1101 60-61 0.1524034713295066 1101 62-63 0.040252133971449666 1101 64-65 0.07352091642581371 1101 66-67 0.02525503823733999 1101 68-69 0.11577682564701064 1101 70-71 0.03732006837545754 1101 72-73 -0.016349453072173503 1101 74-75 -0.10176393074199552 1101 76-77 -0.18469321701734742 1101 78-79 -0.0663736695929984 1101 80-81 -0.016619690269521925 1101 82-83 -0.0013949247960098887 1101 84-85 0.003326369829125042 1101 86-87 0.026435303090714513 1101 88-89 -0.10135074034221958 1101 90-91 0.04340039751950542 1101 92-93 0.0409071426670522 1101 94-95 -0.11847096289281467 1101 96-97 -0.1151097276000641 1101 98-99 -0.09072253936579244 1101 100-101 -0.2086808585744997 1102 1 0.40969872210693126 1102 2 0.3218735958473218 1102 3 0.30986484878116727 1102 4 0.2472903341006969 1102 5 0.21483360262534035 1102 6 0.2084037312708773 1102 7 0.24160823054572944 1102 8 0.2382884800864531 1102 9 0.1991775755871572 1102 10-11 0.22734687981859025 1102 12-13 0.30839928212872536 1102 14-15 0.4477540736951653 1102 16-17 0.39451410245587937 1102 18-19 0.3728895667797687 1102 20-21 0.37677494432127645 1102 22-23 0.4093648946636108 1102 24-25 0.3736158403954306 1102 26-27 0.35434484635838004 1102 28-29 0.3218448689873554 1102 30-31 0.43568983919468707 1102 32-33 0.3806569718147159 1102 34-35 0.4165338797225573 1102 36-37 0.35800155290024804 1102 38-39 0.43256431788242367 1102 40-41 0.27038049361568994 1102 42-43 0.40278128787466727 1102 44-45 0.42033465686294846 1102 46-47 0.3889458478791923 1102 48-49 0.31003092063640025 1102 50-51 0.2521144348674369 1102 52-53 0.3894945119848998 1102 54-55 0.3397803814818303 1102 56-57 0.35441306853405763 1102 58-59 0.36883974281634124 1102 60-61 0.31878017871782305 1102 62-63 0.31832455130121673 1102 64-65 0.3341768382726471 1102 66-67 0.32499962590903664 1102 68-69 0.24144396872200247 1102 70-71 0.2385147463165751 1102 72-73 0.298671547751006 1102 74-75 0.2299009938832839 1102 76-77 0.11198900230746034 1102 78-79 0.26238464183828825 1102 80-81 0.339532915821124 1102 82-83 0.22301559746048127 1102 84-85 0.2460208164232327 1102 86-87 0.2937931046769364 1102 88-89 0.32406066387374466 1102 90-91 0.30244733924911316 1102 92-93 0.29583261366312286 1102 94-95 0.29623611129725447 1102 96-97 0.20860792912407078 1102 98-99 0.16346872730891704 1102 100-101 0.23361479605265245 1103 1 0.2616010867566487 1103 2 0.06282010651311509 1103 3 0.08047569725672332 1103 4 0.17468299053155079 1103 5 0.11767053150921214 1103 6 0.16920549023186027 1103 7 0.1765824550735644 1103 8 0.14715068706186685 1103 9 0.168072228513104 1103 10-11 0.2323811154096589 1103 12-13 0.1442097568715468 1103 14-15 0.28154574094288165 1103 16-17 0.24576801293666506 1103 18-19 0.09455118991401434 1103 20-21 0.11671135397697441 1103 22-23 0.1283415921492832 1103 24-25 0.19370350953970927 1103 26-27 0.27446505442151903 1103 28-29 0.20167957127948455 1103 30-31 0.13908457811634634 1103 32-33 0.07453686601674292 1103 34-35 0.07618701484739177 1103 36-37 0.15871136617334258 1103 38-39 0.13046881150712153 1103 40-41 0.06758679500975262 1103 42-43 0.13520120727112328 1103 44-45 0.12288570810245147 1103 46-47 0.10896053687685026 1103 48-49 0.11869045663141975 1103 50-51 0.09310650001965826 1103 52-53 0.18818319355439428 1103 54-55 0.16886785074417077 1103 56-57 0.17801075734113425 1103 58-59 0.06364048842242198 1103 60-61 0.1593603941254642 1103 62-63 0.0386874067308014 1103 64-65 0.16447010690001207 1103 66-67 0.20823371553981218 1103 68-69 0.21267863475647886 1103 70-71 0.07888857201023569 1103 72-73 0.13342218342203438 1103 74-75 0.011866530519789364 1103 76-77 0.013047446854166367 1103 78-79 0.10900872900540293 1103 80-81 0.20787208291777404 1103 82-83 0.046183307696757936 1103 84-85 0.15090816203768043 1103 86-87 0.1171597671600253 1103 88-89 0.11350364406684577 1103 90-91 0.08869267340057263 1103 92-93 0.11400281080248931 1103 94-95 0.004745503423762898 1103 96-97 0.1593821229723531 1103 98-99 0.1505865693278139 1103 100-101 -2.4867959769636627E-5 1104 1 0.20949939288025377 1104 2 0.09647085012878875 1104 3 0.10011748333532466 1104 4 0.1136981868224467 1104 5 0.09923096423793254 1104 6 0.04591808288282806 1104 7 0.11992318735003238 1104 8 0.11817922771884071 1104 9 0.14111993637305886 1104 10-11 0.2644089074161755 1104 12-13 0.1962313320146052 1104 14-15 0.2512415962213055 1104 16-17 0.2815342413582371 1104 18-19 0.15908310825420102 1104 20-21 0.20957947328820836 1104 22-23 0.16563226554066546 1104 24-25 0.1698298782347365 1104 26-27 0.11067872188937855 1104 28-29 0.12304675587218128 1104 30-31 0.09857821020937507 1104 32-33 0.09843939260258594 1104 34-35 -0.008375900248644541 1104 36-37 -0.0025742111412441204 1104 38-39 0.0881381006394335 1104 40-41 0.029980024757882973 1104 42-43 0.11780099449678261 1104 44-45 0.04596471918335254 1104 46-47 0.15286910520325137 1104 48-49 0.1529423096507614 1104 50-51 0.06748689325816315 1104 52-53 0.10999300131321377 1104 54-55 0.09439113990275416 1104 56-57 0.11601374318682645 1104 58-59 0.08458869144241987 1104 60-61 0.18222487963623024 1104 62-63 0.32136205900236803 1104 64-65 0.16505457962681191 1104 66-67 0.14403584415884296 1104 68-69 0.03421131050327375 1104 70-71 0.1430588615321895 1104 72-73 0.20228182678422257 1104 74-75 0.18022663576865483 1104 76-77 0.02475660271794311 1104 78-79 0.12188201444261892 1104 80-81 0.05889020376133658 1104 82-83 -0.06342087073597469 1104 84-85 0.08515344794773227 1104 86-87 0.10302866139016231 1104 88-89 -0.01376912300654709 1104 90-91 -0.029768346427715642 1104 92-93 0.056078717829734614 1104 94-95 0.21776939456736244 1104 96-97 0.02589424366706794 1104 98-99 -0.05263306545334956 1104 100-101 0.05196518505869463 1105 1 0.275514425160992 1105 2 0.14380447528754559 1105 3 0.14981123022322862 1105 4 0.16863334072763791 1105 5 0.15772789320068625 1105 6 0.15801666752953736 1105 7 0.24007150794707854 1105 8 0.2149514161019681 1105 9 0.32699440825737724 1105 10-11 0.24158796324970666 1105 12-13 0.2006744721869893 1105 14-15 0.29935075070622474 1105 16-17 0.38356877490539887 1105 18-19 0.31010816140110364 1105 20-21 0.33591632488451495 1105 22-23 0.3415601362423786 1105 24-25 0.3578050351236115 1105 26-27 0.27695050819465195 1105 28-29 0.19789294228318965 1105 30-31 0.19175492620500023 1105 32-33 0.30129813666711414 1105 34-35 0.3464448790299244 1105 36-37 0.3615746760944063 1105 38-39 0.3470047514716228 1105 40-41 0.3883805728138725 1105 42-43 0.37295570450017834 1105 44-45 0.3020884727910911 1105 46-47 0.39024323913279346 1105 48-49 0.4987009608676942 1105 50-51 0.2150458652213274 1105 52-53 0.3333400491326657 1105 54-55 0.36539717205196354 1105 56-57 0.39563745851701526 1105 58-59 0.4162497538832426 1105 60-61 0.4235913292659248 1105 62-63 0.37625124713227365 1105 64-65 0.3027605177506558 1105 66-67 0.27467630280894184 1105 68-69 0.23656737546648543 1105 70-71 0.2809095673113191 1105 72-73 0.33162493823208905 1105 74-75 0.3062266776449505 1105 76-77 0.21725276351244105 1105 78-79 0.2953578318645995 1105 80-81 0.32895909026414927 1105 82-83 0.28501769968588064 1105 84-85 0.3876282060303424 1105 86-87 0.3614667345310423 1105 88-89 0.4225488122828054 1105 90-91 0.4157198428217157 1105 92-93 0.35250742245857225 1105 94-95 0.32620723869725055 1105 96-97 0.2427666603414238 1105 98-99 0.2465967706205916 1105 100-101 0.26895750533905627 1106 1 0.18781765327013744 1106 2 -0.05431197785120645 1106 3 -0.014104248542736286 1106 4 0.023604871054700993 1106 5 0.1034016496899568 1106 6 0.10170224998206834 1106 7 0.1363797921773724 1106 8 0.06581452587416692 1106 9 0.07824072150238237 1106 10-11 0.14312696199856134 1106 12-13 0.16596067003285953 1106 14-15 0.19289839794771524 1106 16-17 0.20980713158871822 1106 18-19 0.2199606396606839 1106 20-21 0.16916805245699607 1106 22-23 0.14341759073535343 1106 24-25 0.18731138040102024 1106 26-27 0.20292033897436568 1106 28-29 0.18303929792023865 1106 30-31 0.13632152874672698 1106 32-33 0.10236766606386993 1106 34-35 -0.05574417402971221 1106 36-37 0.04246887766149854 1106 38-39 0.12238586111239869 1106 40-41 0.02586416039209638 1106 42-43 -0.0033990048179575183 1106 44-45 0.025124426141807987 1106 46-47 -0.02743630535243824 1106 48-49 0.051411088185567166 1106 50-51 0.05371095389027403 1106 52-53 0.1809957303754146 1106 54-55 0.04342894418119414 1106 56-57 0.08874482120351956 1106 58-59 0.09885316663616095 1106 60-61 0.2347109302395225 1106 62-63 0.31528691185629043 1106 64-65 0.16888862934611382 1106 66-67 0.1221019846572915 1106 68-69 0.14059191388927417 1106 70-71 0.1310153541476211 1106 72-73 0.17381926130585867 1106 74-75 0.12820328084381316 1106 76-77 -0.005245236072660475 1106 78-79 0.033899738785059697 1106 80-81 0.034288955782152186 1106 82-83 -0.05513046631054408 1106 84-85 0.0387719537190776 1106 86-87 0.1607970948376547 1106 88-89 0.03358730142515043 1106 90-91 0.002873184770905368 1106 92-93 -0.022647360047045595 1106 94-95 0.1462265921476984 1106 96-97 0.15435089874706875 1106 98-99 0.12299142381113626 1106 100-101 0.17609154537828076 1107 1 0.13589261700226274 1107 2 0.02454618531236008 1107 3 0.05116325933595789 1107 4 0.10430321886052951 1107 5 0.09458992966246882 1107 6 0.045356025492871765 1107 7 0.09382975424338724 1107 8 0.08752273331626981 1107 9 0.04760723200793393 1107 10-11 0.10830234068526323 1107 12-13 0.18430466913289933 1107 14-15 0.12704721858209922 1107 16-17 0.17614238907861335 1107 18-19 0.20316319006116856 1107 20-21 0.1745158641487663 1107 22-23 0.08534780927027441 1107 24-25 0.07153156383834869 1107 26-27 0.21296951186428004 1107 28-29 0.0718386167613474 1107 30-31 0.11714194240040143 1107 32-33 0.12161776216507292 1107 34-35 0.11846956889465332 1107 36-37 0.1953690811667812 1107 38-39 0.22860980649172546 1107 40-41 0.1251386963206116 1107 42-43 0.10667112106528265 1107 44-45 0.2580755939379529 1107 46-47 0.253484688000718 1107 48-49 0.17588919603962694 1107 50-51 0.1944602873131842 1107 52-53 0.2299678874194342 1107 54-55 0.246726561457983 1107 56-57 0.19910604974202784 1107 58-59 0.3259882793211517 1107 60-61 0.30366014029034005 1107 62-63 0.2790704892946607 1107 64-65 0.17780564749524075 1107 66-67 0.2541938131498398 1107 68-69 0.18630062889497623 1107 70-71 0.22392161962577717 1107 72-73 0.27820262217664293 1107 74-75 0.38252343618386675 1107 76-77 0.2832469588040425 1107 78-79 0.3744025413145593 1107 80-81 0.27578953132302786 1107 82-83 0.20384066707578796 1107 84-85 0.26615298416916744 1107 86-87 0.2650242412798818 1107 88-89 0.1689856242260106 1107 90-91 0.12952456588239158 1107 92-93 0.16011619048832415 1107 94-95 0.15810115462733876 1107 96-97 0.29606191058470444 1107 98-99 0.3328156585870303 1107 100-101 0.2817983303627152 1108 1 0.08719484293918356 1108 2 -0.06783772235817054 1108 3 0.020091088511072996 1108 4 0.08711313198142534 1108 5 0.05808020900138189 1108 6 0.034420081623245835 1108 7 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-0.5969441594942779 2316 58-59 -0.5498502078228285 2316 60-61 -0.6462782756441356 2316 62-63 -0.5643742186809959 2316 64-65 -0.49059079315136245 2316 66-67 -0.5709242619135679 2316 68-69 -0.4146916315112392 2316 70-71 -0.470313027812459 2316 72-73 -0.4453166969035678 2316 74-75 -0.38902304343171323 2316 76-77 -0.4443225171800478 2316 78-79 -0.45006885433549115 2316 80-81 -0.318624728518742 2316 82-83 -0.44583571286519685 2316 84-85 -0.4451728768396208 2316 86-87 -0.5309568143775323 2316 88-89 -0.6075146813580474 2316 90-91 -0.5437490710912272 2316 92-93 -0.5132677184083789 2316 94-95 -0.4693984201716006 2316 96-97 -0.5261096613135194 2316 98-99 -0.5909191579971136 2316 100-101 -0.32564016500493054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 1.0 11 1.0 12 0.0 13 5.0 14 42.0 15 126.0 16 368.0 17 790.0 18 1381.0 19 2136.0 20 3034.0 21 4154.0 22 5518.0 23 6132.0 24 6655.0 25 7921.0 26 9669.0 27 12051.0 28 15291.0 29 19090.0 30 23817.0 31 29864.0 32 37755.0 33 49528.0 34 69714.0 35 107236.0 36 199065.0 37 440656.0 38 636264.0 39 188502.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48272116935205 19.367659707811207 13.291744339601527 23.85787478323522 2 14.823712136404948 20.27982471486297 41.234836427562605 23.66162672116948 3 17.77517057799914 26.696216114838982 29.835245575841274 25.693367731320606 4 11.858674143007395 17.18966952144386 39.49087142633364 31.4607849092151 5 13.130696367431883 38.41955740830134 35.57458385148765 12.875162372779128 6 30.64100439633864 38.11057076741179 18.476291154732895 12.772133681516676 7 26.972591970793193 32.21931753074576 23.579859029193482 17.228231469267566 8 25.338092360940166 36.41316799282203 20.66037102513035 17.58836862110745 9 25.99509604902672 15.59375422915155 20.27611098146153 38.1350387403602 10-11 24.29091447709838 26.378290479707 29.398211048914835 19.93258399427979 12-13 25.12992486362818 23.60143623318596 29.449307191715494 21.819331711470365 14-15 22.41615490602892 25.176385589928678 26.306441523954447 26.101017980087953 16-17 22.56629977611471 28.224385061384638 26.8113289579264 22.39798620457425 18-19 22.451213573352003 27.59458114483066 28.161913499931266 21.79229178188607 20-21 23.334633381179355 26.77443210819808 27.734895735097137 22.156038775525428 22-23 22.974855795922323 26.660091834527357 27.554700578734415 22.810351790815908 24-25 22.60428887915629 26.85970778760628 27.70519177304148 22.830811560195947 26-27 22.73096362316941 27.08039623753366 27.40194495682669 22.78669518247024 28-29 22.791670618373786 26.865649772541516 27.596458062707995 22.746221546376702 30-31 22.817011871994307 26.854885888833124 27.599669446850957 22.72843279232162 32-33 22.79612585741885 26.89047736265048 27.48676775071476 22.826629029215912 34-35 22.988575563149823 27.073602954731697 27.49039083487581 22.447430647242673 36-37 22.916019528434035 26.814167119108422 27.45907644338688 22.810736909070663 38-39 22.865044377452907 26.9322760321261 27.43748314999462 22.765196440426372 40-41 22.864591491932774 26.96219311677946 27.379567084067276 22.793648307220487 42-43 22.857052280038832 26.838075843938846 27.391608510837816 22.913263365184505 44-45 22.904285575756024 26.978763398751315 27.322823192427325 22.794127833065332 46-47 22.940543057691222 26.888745741544096 27.175395635462323 22.995315565302363 48-49 23.01689422832037 26.88717396238599 27.246418741148755 22.849513068144887 50-51 22.961642194864385 27.032869898244616 27.106343913804697 22.8991439930863 52-53 22.895361066976974 26.98864695922006 27.113723283750364 23.00226869005261 54-55 22.793568386246346 26.94258583779026 27.23645525970587 23.027390516257523 56-57 22.832063655457482 27.122008424736286 27.052397256259674 22.99353066354655 58-59 22.872130703826723 26.966642051006634 27.19521603704923 22.96601120811741 60-61 23.05065366050147 27.06532502403624 27.169382160088677 22.714639155373607 62-63 22.858608842012387 27.104534121463846 27.171374963269507 22.86548207325426 64-65 22.899587925700885 26.90766850343608 27.208252568404657 22.98449100245838 66-67 22.79018506500772 27.077838766361157 27.108128815560505 23.023847353070618 68-69 22.777317788171057 26.93819018421252 27.12216826668457 23.16232376093186 70-71 22.944885430619536 26.792307766080896 27.071711491677032 23.191095311622533 72-73 22.810032106919344 27.026449579988643 26.9781506712829 23.185367641809112 74-75 22.811630526402162 26.987554705906796 27.04515108793758 23.15566367975346 76-77 22.900342807698415 26.90057404571693 27.06164144893529 23.137441697649365 78-79 22.862806590176966 26.800512986092684 27.13048004799521 23.20620037573514 80-81 22.892324069959624 27.012569970812862 26.863543994365042 23.231561964862475 82-83 22.7985767672925 26.884296807316925 27.13002716247508 23.187099262915496 84-85 22.783818027401175 26.944903546040344 26.95478710650908 23.3164913200494 86-87 22.845570300087275 26.907074284947047 27.02000262140795 23.227352793557728 88-89 22.756165370348466 26.86415672183345 26.964430904055405 23.41524700376268 90-91 22.747107659945843 26.8858153058256 27.040275908514992 23.326801125713562 92-93 22.954529228165857 26.74691265276894 27.034361756428577 23.264196362636625 94-95 22.798523486643074 26.780985628077623 27.06326650874282 23.357224376536482 96-97 22.73141650868954 26.625832376945674 27.18205571664072 23.460695397724063 98-99 22.760691839313914 26.616723108694774 27.06302894421084 23.559556107780473 100-101 22.637069005707737 26.62449466189801 27.02879178405163 23.709644548342624 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 6.0 2 14.5 3 29.0 4 25.0 5 20.5 6 38.0 7 90.0 8 168.5 9 212.5 10 211.5 11 213.0 12 238.5 13 289.0 14 325.0 15 355.5 16 393.0 17 439.0 18 487.0 19 548.5 20 669.5 21 863.5 22 1066.5 23 1316.0 24 1712.5 25 2372.5 26 3502.5 27 5345.0 28 8097.0 29 11941.0 30 16731.5 31 21787.5 32 26846.0 33 32452.0 34 38047.5 35 42707.0 36 47339.5 37 51208.0 38 54912.0 39 59589.0 40 64296.5 41 68354.5 42 69631.0 43 71230.0 44 73800.0 45 74960.5 46 76296.5 47 77321.5 48 77643.5 49 76781.0 50 74657.5 51 71979.0 52 69578.0 53 68810.5 54 67406.5 55 62979.5 56 56108.0 57 49283.0 58 43604.0 59 37111.5 60 30484.0 61 24385.0 62 18385.5 63 12769.5 64 8439.0 65 5663.5 66 3781.5 67 2631.5 68 1866.5 69 1272.0 70 865.0 71 579.0 72 401.5 73 303.5 74 225.0 75 148.0 76 79.5 77 50.5 78 32.5 79 18.0 80 9.0 81 5.5 82 4.0 83 2.0 84 2.0 85 2.0 86 2.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021099137173163175 2 0.10474975677383536 3 0.0 4 0.0 5 0.0 6 0.003463242212766683 7 0.03084949601833707 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0014918581839610326 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.3280649427179735E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 2.6640324713589868E-5 62-63 3.99604870703848E-4 64-65 4.262451954174379E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.0656129885435947E-4 100-101 8.791307155484656E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1876854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.17187727820216 #Duplication Level Percentage of deduplicated Percentage of total 1 65.69871560814042 32.30529181218985 2 16.698832376396226 16.42225872602848 3 6.849163972951924 10.103587510088268 4 3.5993305300844165 7.079433564359889 5 2.05161313613912 5.044083467629014 6 1.3230556385364327 3.9034277694208126 7 0.8676782235017937 2.9865756986099066 8 0.5980204396221319 2.352463013356478 9 0.4382760124243493 1.9395768867218919 >10 1.8031236910246644 14.550534171508648 >50 0.05340750638752598 1.755820082908622 >100 0.018346232515081103 1.355826803408416 >500 2.1831613800556696E-4 0.06757691530719362 >1k 2.1831613800556656E-4 0.13354357846250792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3280649427179735E-5 5.3280649427179735E-5 10-11 0.0 0.0 0.0 7.99209741407696E-5 5.3280649427179735E-5 12-13 0.0 0.0 0.0 1.3320162356794934E-4 5.3280649427179735E-5 14-15 0.0 0.0 0.0 2.1312259770871894E-4 5.3280649427179735E-5 16-17 0.0 0.0 0.0 2.664032471358987E-4 5.3280649427179735E-5 18-19 0.0 0.0 0.0 2.664032471358987E-4 5.3280649427179735E-5 20-21 0.0 0.0 0.0 5.061661695582075E-4 7.99209741407696E-5 22-23 0.0 0.0 0.0 7.459290919805163E-4 1.3320162356794934E-4 24-25 0.0 0.0 0.0 8.524903908348758E-4 1.598419482815392E-4 26-27 0.0 0.0 0.0 0.0010922533132571846 1.598419482815392E-4 28-29 0.0 0.0 0.0 0.0016783404569561617 1.598419482815392E-4 30-31 0.0 0.0 0.0 0.0027439534454997564 1.598419482815392E-4 32-33 0.0 0.0 0.0 0.005701029488708232 1.598419482815392E-4 34-35 0.0 0.0 0.0 0.009723718520460303 2.1312259770871894E-4 36-37 0.0 0.0 0.0 0.017236290089692644 2.1312259770871894E-4 38-39 0.0 0.0 0.0 0.03284752037185631 2.1312259770871894E-4 40-41 0.0 0.0 0.0 0.0520551944903546 2.664032471358987E-4 42-43 0.0 0.0 0.0 0.07304777036466342 3.196838965630784E-4 44-45 0.0 0.0 0.0 0.09747694812702533 3.7296454599025815E-4 46-47 0.0 0.0 0.0 0.12486320193259573 4.262451954174379E-4 48-49 0.0 0.0 0.0 0.1564586270429133 4.795258448446176E-4 50-51 0.0 0.0 0.0 0.19154393469071115 4.795258448446176E-4 52-53 0.0 0.0 0.0 0.23038552812312518 5.594468189853872E-4 54-55 0.0 0.0 0.0 0.2719177943516118 6.393677931261568E-4 56-57 0.0 0.0 0.0 0.31832524000268536 6.393677931261568E-4 58-59 0.0 0.0 0.0 0.37208541527470973 6.660081178397467E-4 60-61 0.0 0.0 0.0 0.4350365025729226 7.459290919805163E-4 62-63 0.0 0.0 0.0 0.5003052981212177 9.057710402620555E-4 64-65 0.0 0.0 0.0 0.5687176519857164 9.057710402620555E-4 66-67 0.0 0.0 0.0 0.6474664518390881 9.057710402620555E-4 68-69 0.0 0.0 0.0 0.7398284576211043 9.057710402620555E-4 70-71 0.0 0.0 0.0 0.8408752092597507 0.0011188936379707744 72-73 0.0 0.0 0.0 0.9491414888957799 0.0013852968851066731 74-75 0.0 0.0 0.0 1.0657994708165899 0.0013852968851066731 76-77 0.0 0.0 0.0 1.1911155582693165 0.001411937209820263 78-79 0.0 0.0 0.0 1.3293522032081344 0.0014385775345338529 80-81 0.0 0.0 0.0 1.4885547836965476 0.0014652178592474427 82-83 0.0 0.0 0.0 1.6621964201797264 0.0014918581839610326 84-85 0.0 0.0 0.0 1.8470269930426126 0.0014918581839610326 86-87 0.0 0.0 0.0 2.0443785185208867 0.0015451388333882123 88-89 0.0 0.0 0.0 2.2572613533071832 0.0015451388333882123 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 41.943382 1 GTATCAA 3630 0.0 39.723557 1 TCAACGC 4085 0.0 34.780113 4 ATCAACG 4110 0.0 34.568554 3 CAACGCA 4120 0.0 34.48465 5 AACGCAG 4305 0.0 33.113106 6 TATCAAC 4440 0.0 32.14484 2 ACGCAGA 4995 0.0 27.968147 7 CGCAGAG 5055 0.0 27.436481 8 GTACATG 5880 0.0 26.546583 1 TACATGG 6115 0.0 24.58463 2 ACATGGG 6230 0.0 23.186617 3 GCAGAGT 6345 0.0 21.558947 9 AGAGTAC 5375 0.0 20.280138 10-11 ACCCGTG 260 1.48538675E-8 20.103477 7 TATACAG 1295 0.0 19.814167 5 TTACGTA 145 6.1329565E-4 19.662336 4 ATAGTCG 145 6.1505905E-4 19.653955 8 CATGGGG 3950 0.0 19.12722 4 CGTATAC 155 9.506513E-4 18.421782 1 >>END_MODULE