FastQCFastQC Report
Fri 10 Feb 2017
SRR2568667_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568667_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958278
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6250.038.764411
GTATCAA8500.038.004321
GTACATG34900.033.3490641
TACATGG35100.032.617672
TCAACGC10500.030.3098074
GTATAGT1102.7304031E-630.2307111
CTATACT2101.8189894E-1229.4050374
ACATGGG37500.029.3884743
CAACGCA11400.027.9169255
GTATAGC1754.953108E-927.1459451
ATCAACG11900.026.7453443
TATCAAC12100.026.697252
AACGCAG12350.025.7694726
CATGGGG29700.024.7897954
CCTATAC2151.6607373E-924.3038223
TATACTG2351.7644197E-1024.2555475
AGTACAT22600.024.1731052
TACACCG1852.5404916E-723.1083225
TGTACGC1452.3317689E-522.9336432
ATACTAC1252.2553801E-422.8002136
Produced by FastQC (version 0.11.5)