FastQCFastQC Report
Fri 10 Feb 2017
SRR2568667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958278
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA608.221186E-539.6391372
GGTATCA6200.037.595211
GTACATG31900.036.385411
TACATGG31250.035.922572
GTATCAA8950.034.5476461
GTATATA1001.2841483E-633.2986151
ACATGGG33600.031.9610423
CTATACT1950.031.6782044
TCAACGC9450.031.1753754
CGTATAC953.481577E-530.010933
TATACTG2550.029.814785
GTACGTA650.005802018629.2735081
CATGGGG24550.029.226534
ATCAACG10550.028.3752633
CAACGCA10650.027.6626575
TATCAAC11550.026.3574542
GTACCGT2007.1486284E-1026.134526
GTATAGA2407.2759576E-1225.7667871
AACGCAG11450.025.7298956
GTAATAC1701.1184784E-725.1562213
Produced by FastQC (version 0.11.5)