FastQCFastQC Report
Fri 10 Feb 2017
SRR2568666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319885
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38150.035.7350044
ATCAACG40350.033.786123
GGTATCA23750.033.600491
CAACGCA40750.033.4549835
GTATCAA44100.032.8519551
AACGCAG43450.031.3760766
GTACATG86500.027.8414481
TATCAAC51000.027.1041532
ACATGGG82700.026.9381223
ACGCAGA51750.025.8848257
CGCAGAG52350.025.134468
TACATGG95150.024.3626232
CATGGGG47550.020.7787464
GCAGAGT70300.018.716779
GAGTACA62700.017.8032911
TAATACT32900.017.6145044
CTTAATA28500.016.8340872
CCTATAC16950.016.8144053
CATGGGA39450.016.736874
TATACTG30350.016.4337655
Produced by FastQC (version 0.11.5)