FastQCFastQC Report
Fri 10 Feb 2017
SRR2568666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319885
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC36260.10922065071531092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.036.4872741
TCAACGC40150.033.490674
ATCAACG41250.032.943143
GTATCAA43300.032.6269071
CAACGCA41400.032.4794775
AACGCAG43550.030.9851156
GTACATG80800.027.963381
ACATGGG77450.027.4839763
ACGCAGA51750.025.983197
TATCAAC53550.025.490062
CGCAGAG52950.025.1168148
TACATGG90850.024.0813792
GTATAGG17400.020.229721
CATGGGG44150.019.4791874
GCACCGT5050.017.876616
GTATATA30200.017.7983151
GCAGAGT74000.017.779549
CATGGGA39450.017.7048934
GAGTACA60350.017.261331
TGTACCG4455.456968E-1217.0837385
Produced by FastQC (version 0.11.5)