FastQCFastQC Report
Fri 10 Feb 2017
SRR2568665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900073
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC46870.5207355403395059No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC34370.38185791596903806No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA29050.32275159903696704No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT23820.2646452010003633No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG23740.2637563842043923No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA22140.24598004828497244No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC21420.2379806971212335No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT21190.23542534883281688No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT20390.22653718087310695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.21942664650533902No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA19360.21509366462498042No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT19060.2117606016400892No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT18530.20587219036678137No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG18070.20076149378994818No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT17000.1888735691438361No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT16740.1859849145569304No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG16060.17842997179117692No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA15940.17709674659722044No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT15080.16754196604053226No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA15070.16743086394103587No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT15040.16709755764254677No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT14970.16631984294607216No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG13960.15509853089693837No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC13760.15287648890701086No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC13500.14998783432010515No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG13430.1492101196236305No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC13190.14654366923571754No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG12950.14387721884780458No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG12880.14309950415132996No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA12800.14221068735535894No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG12740.1415440747583807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.13709999077852575No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT12200.13554456138557652No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG12070.13410023409212363No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC11970.13298921309715991No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA11880.13198929420169253No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG11660.1295450480127723No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC11530.12810072071931944No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA11500.1277674144208303No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG10650.11832373596363852No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC10540.1171016128691784No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10240.11376854988428715No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA10220.11354634568529441No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC10200.11332414148630167No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT10120.11243532469033066No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC10090.11210201839184156No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT9980.11087989529738143No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC9670.10743573021299384No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT9390.10432487142709537No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC9250.10276944203414613No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC9230.10254723783515336No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9180.1019917273376715No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG9160.10176952313867875No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC9090.10099180844220412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT3750.062.06541
GTCTAAT601.4921316E-647.499032
CATATAG5050.046.0907253
ACATATA5450.043.577092
CCGAAGA459.624931E-442.221369
GGGTCAC704.309757E-640.713458
ATGCCCC608.290318E-539.5825279
AACGTTC308.296341E-539.58032620-21
CGACTTA250.00161666337.99711222-23
CATTGCG250.001617104337.99516-17
GTCTTCG651.3281159E-436.5377161
CATGGGG19400.036.236374
GTATCAA33700.036.2232931
GTACATG56300.035.940651
ATTGAAC2200.034.5447468
GGTATCA19000.034.24931
ACATGGG54250.033.8859833
CCCTATA2900.032.7579542
ATGGGGT6500.032.1531875
TATAAGC5650.031.9462472
Produced by FastQC (version 0.11.5)