##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568665_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 900073 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3366649149569 34.0 31.0 34.0 30.0 34.0 2 32.47605138694306 34.0 31.0 34.0 31.0 34.0 3 32.50896760596085 34.0 31.0 34.0 31.0 34.0 4 35.867845163670054 37.0 35.0 37.0 35.0 37.0 5 35.9213330474306 37.0 37.0 37.0 35.0 37.0 6 36.04457416231794 37.0 37.0 37.0 35.0 37.0 7 36.02644563274312 37.0 37.0 37.0 35.0 37.0 8 36.04686619863055 37.0 37.0 37.0 35.0 37.0 9 37.87911980472695 39.0 39.0 39.0 35.0 39.0 10-11 37.84598582559414 39.0 39.0 39.0 35.0 39.0 12-13 37.84895614022418 39.0 39.0 39.0 35.0 39.0 14-15 39.27294230579075 41.0 39.0 41.0 36.0 41.0 16-17 39.25779520105591 41.0 39.0 41.0 36.0 41.0 18-19 39.297820287909985 41.0 39.5 41.0 36.0 41.0 20-21 39.32269938105021 41.0 40.0 41.0 36.0 41.0 22-23 39.253739418913796 41.0 39.0 41.0 36.0 41.0 24-25 39.170332850779886 41.0 39.0 41.0 36.0 41.0 26-27 39.08079622430625 41.0 39.0 41.0 36.0 41.0 28-29 38.983704655066866 41.0 39.0 41.0 35.0 41.0 30-31 38.883225582813836 40.5 39.0 41.0 35.0 41.0 32-33 38.76266647260833 40.0 38.5 41.0 35.0 41.0 34-35 38.65662340721253 40.0 38.0 41.0 35.0 41.0 36-37 38.54499690580653 40.0 38.0 41.0 34.5 41.0 38-39 38.43504915712393 40.0 38.0 41.0 34.0 41.0 40-41 38.36351384832119 40.0 38.0 41.0 34.0 41.0 42-43 38.22743932992102 40.0 38.0 41.0 34.0 41.0 44-45 38.027983285800154 40.0 38.0 41.0 33.0 41.0 46-47 38.084070958688905 40.0 38.0 41.0 33.5 41.0 48-49 38.221683130146104 40.0 38.0 41.0 34.0 41.0 50-51 37.12527317228714 39.0 36.5 40.0 32.0 40.5 52-53 37.29312233563277 39.5 37.5 40.0 33.0 40.5 54-55 37.93877718807252 40.0 38.0 41.0 33.0 41.0 56-57 37.952388861792315 40.0 38.0 41.0 33.0 41.0 58-59 37.84077069304379 40.0 38.0 41.0 33.0 41.0 60-61 37.63977310729241 40.0 37.0 41.0 32.5 41.0 62-63 37.3868513998309 40.0 37.0 41.0 32.0 41.0 64-65 37.24029773140623 39.0 36.0 41.0 32.0 41.0 66-67 37.11620446341574 39.0 36.0 41.0 32.0 41.0 68-69 36.802951538375225 39.0 35.5 41.0 32.0 41.0 70-71 36.42685871034905 38.0 35.0 40.5 31.0 41.0 72-73 36.06046898418239 37.0 35.0 39.5 31.0 41.0 74-75 35.63527291675231 37.0 35.0 39.0 31.0 41.0 76-77 35.262169846223586 36.0 35.0 39.0 31.0 41.0 78-79 34.87464016807526 36.0 35.0 38.0 30.5 39.5 80-81 34.46183365127051 35.5 34.5 37.0 30.0 39.0 82-83 34.10936224061826 35.0 34.0 37.0 30.0 39.0 84-85 33.73456153000923 35.0 34.0 36.0 29.0 37.5 86-87 33.509948637499406 35.0 34.0 36.0 29.0 37.0 88-89 33.29713478795608 35.0 34.0 36.0 29.0 37.0 90-91 33.11854594016263 35.0 34.0 35.0 29.0 36.0 92-93 32.96557779202354 35.0 34.0 35.0 29.0 36.0 94-95 32.81237355192302 35.0 34.0 35.0 29.0 36.0 96-97 32.71465647786346 35.0 34.0 35.0 28.5 36.0 98-99 32.57717429586267 35.0 34.0 35.0 27.0 35.5 100-101 31.28227210459596 34.5 31.5 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13983589816456288 1101 2 0.1478195253622303 1101 3 0.18232947948188638 1101 4 0.07895518892013342 1101 5 0.12248860179578713 1101 6 0.0012394186766542248 1101 7 0.20806263783441636 1101 8 0.19849814292299328 1101 9 0.19289149293162922 1101 10-11 0.2555976705545504 1101 12-13 0.2660399783211105 1101 14-15 0.381544623879698 1101 16-17 0.4202271501966024 1101 18-19 0.32414228505026443 1101 20-21 0.19881980159042456 1101 22-23 0.12962605330887556 1101 24-25 0.19787077521998953 1101 26-27 0.22462097908952927 1101 28-29 0.24080371955329838 1101 30-31 0.2767236695659605 1101 32-33 0.27867279876850404 1101 34-35 0.0717420150924255 1101 36-37 0.04493892169303848 1101 38-39 0.1206657370436659 1101 40-41 0.25557071893783245 1101 42-43 0.3668318297359505 1101 44-45 0.41718553416657045 1101 46-47 0.3927907515468121 1101 48-49 0.46960438550946293 1101 50-51 0.32714590184921377 1101 52-53 0.37132323441220905 1101 54-55 0.3625675058734217 1101 56-57 0.40378654491618704 1101 58-59 0.3921236587329062 1101 60-61 0.40787210748974445 1101 62-63 0.44105332398148533 1101 64-65 0.45084154789095265 1101 66-67 0.5126923403963843 1101 68-69 0.3165403121542951 1101 70-71 0.30481740791442036 1101 72-73 0.3521896351763161 1101 74-75 0.44332430873592443 1101 76-77 0.3404888910942958 1101 78-79 0.288775810290403 1101 80-81 0.2950868520913943 1101 82-83 0.2897608467980888 1101 84-85 0.17348094008463022 1101 86-87 0.20395549555551185 1101 88-89 0.09257064329760567 1101 90-91 0.12679501324036124 1101 92-93 0.2272028892136433 1101 94-95 0.22759052292661863 1101 96-97 0.09838071646729674 1101 98-99 0.22916598154100143 1101 100-101 0.06012114117994116 1102 1 0.3361925940019006 1102 2 0.21323280537469458 1102 3 0.2985036205917595 1102 4 0.3376674380047646 1102 5 0.1938128376886965 1102 6 0.18084678318884784 1102 7 0.1718470778354586 1102 8 0.2045778905144644 1102 9 0.2960264743645453 1102 10-11 0.22187990327936546 1102 12-13 0.24487284855923974 1102 14-15 0.2725717214413024 1102 16-17 0.18557421192243595 1102 18-19 0.26142967241424486 1102 20-21 0.23343021733739278 1102 22-23 0.33207061172379326 1102 24-25 0.2757178369458231 1102 26-27 0.2793549373849942 1102 28-29 0.39732719458511667 1102 30-31 0.385193442865706 1102 32-33 0.19545199324252138 1102 34-35 0.22721216492753626 1102 36-37 0.063647210664449 1102 38-39 0.1232625325979555 1102 40-41 0.2203013663189637 1102 42-43 0.09494571220498216 1102 44-45 0.108619679431321 1102 46-47 0.10774699554172429 1102 48-49 0.05956712149325938 1102 50-51 0.144820016991126 1102 52-53 0.043763443194563934 1102 54-55 0.23630643703237553 1102 56-57 0.25663100984645837 1102 58-59 0.07878781516387079 1102 60-61 0.23102870724564895 1102 62-63 0.1340487925732461 1102 64-65 0.03293923933970433 1102 66-67 0.1374310378437258 1102 68-69 0.11399837390609235 1102 70-71 0.2217102121939476 1102 72-73 0.1965133646940913 1102 74-75 0.24097063816540754 1102 76-77 0.18085575338622562 1102 78-79 0.18579176759908478 1102 80-81 0.17613089374399493 1102 82-83 0.1274059427496823 1102 84-85 0.12247013741512092 1102 86-87 0.029566067110209815 1102 88-89 0.04527812726239233 1102 90-91 0.03766812082560733 1102 92-93 -0.05554432572311896 1102 94-95 -0.12881317491024902 1102 96-97 -0.061783141332178104 1102 98-99 -0.07230575233938907 1102 100-101 -0.24996059296013584 1103 1 0.24809589313564828 1103 2 0.261862774042136 1103 3 0.3723470806888258 1103 4 0.24849252862343008 1103 5 0.2940186495704964 1103 6 0.2397734895843726 1103 7 0.2146190874195355 1103 8 0.1782001796340822 1103 9 0.1109893053530584 1103 10-11 0.22111502031567198 1103 12-13 0.24364872863542075 1103 14-15 0.25674263745777637 1103 16-17 0.38724374562028885 1103 18-19 0.33871356993837054 1103 20-21 0.23453453631509547 1103 22-23 0.29613032788770255 1103 24-25 0.2938507852778187 1103 26-27 0.2130481855265458 1103 28-29 0.21941511004864367 1103 30-31 0.295930986639128 1103 32-33 0.2806780791305883 1103 34-35 0.24996052150429193 1103 36-37 0.06293301271642804 1103 38-39 0.14076882982716654 1103 40-41 0.1283234219803333 1103 42-43 0.32315883501632214 1103 44-45 0.23269659778236473 1103 46-47 0.18694463638463077 1103 48-49 0.28281157685972147 1103 50-51 0.3586677776349845 1103 52-53 0.20915325704232401 1103 54-55 0.08478588656238628 1103 56-57 0.30141352505196295 1103 58-59 0.21172385988955256 1103 60-61 0.2619235281585901 1103 62-63 0.1532138721333638 1103 64-65 0.08249228965610911 1103 66-67 0.06180285083138415 1103 68-69 0.21118765940095585 1103 70-71 0.15641408882968477 1103 72-73 0.0963152323601264 1103 74-75 0.005761447282573329 1103 76-77 0.07924612016142873 1103 78-79 0.1653442035516548 1103 80-81 0.028227410301091993 1103 82-83 0.11496200344883079 1103 84-85 0.2002347746332802 1103 86-87 0.06625433890476984 1103 88-89 -0.2927417781255812 1103 90-91 -0.20997579390070342 1103 92-93 -0.09996897399982885 1103 94-95 0.021335808317616056 1103 96-97 -0.06330522771173719 1103 98-99 -0.059060068245265995 1103 100-101 -0.21996120732395852 1104 1 0.3793621100531297 1104 2 0.2614043758411384 1104 3 0.369547680354394 1104 4 0.21562624251375695 1104 5 0.2485853335352033 1104 6 0.18987727785630426 1104 7 0.3327762689913456 1104 8 0.25391660701112073 1104 9 0.38649815618624217 1104 10-11 0.18670920817244507 1104 12-13 0.2679135617092854 1104 14-15 0.3193096673833864 1104 16-17 0.3690707075069284 1104 18-19 0.34268923735461243 1104 20-21 0.26689485459912987 1104 22-23 0.27126438773894535 1104 24-25 0.3333278077076258 1104 26-27 0.27906761699745886 1104 28-29 0.47737941841513987 1104 30-31 0.48284069084255776 1104 32-33 0.4174032708505848 1104 34-35 0.22875744245223473 1104 36-37 0.06994592859025772 1104 38-39 0.221619893656964 1104 40-41 0.10110062315659007 1104 42-43 -0.0318042624870003 1104 44-45 0.2575394738707004 1104 46-47 0.015411452967690309 1104 48-49 0.1649109829601727 1104 50-51 0.08596697615888615 1104 52-53 0.21957821235570663 1104 54-55 0.08264957796526318 1104 56-57 0.08516921899187935 1104 58-59 0.07525958035314062 1104 60-61 0.06101393927546894 1104 62-63 -0.04865719976884009 1104 64-65 0.03207281205707346 1104 66-67 -0.01449594407146293 1104 68-69 -0.066982207404088 1104 70-71 0.08094704160603072 1104 72-73 0.07733495213178543 1104 74-75 0.18335471257692149 1104 76-77 0.1682791594279891 1104 78-79 0.07759554415016368 1104 80-81 0.2085638572825914 1104 82-83 -0.006055121616036274 1104 84-85 0.07345595897277946 1104 86-87 0.05577028193653888 1104 88-89 0.024766726746705103 1104 90-91 0.1923536762025151 1104 92-93 -0.015776504415555337 1104 94-95 0.02513993678443427 1104 96-97 0.03751210492967516 1104 98-99 0.11412796150737137 1104 100-101 -0.11497360425644132 1105 1 0.3013479256559677 1105 2 0.2955130427482189 1105 3 0.40294512395739446 1105 4 0.13604937029354147 1105 5 0.3204689733957693 1105 6 0.26996502878790096 1105 7 0.2348478320798577 1105 8 0.3051948055125422 1105 9 0.44709237824819326 1105 10-11 0.3290039551372246 1105 12-13 0.2930217477893464 1105 14-15 0.4284423756416018 1105 16-17 0.3740392534058756 1105 18-19 0.1496271705997927 1105 20-21 0.3022656924402156 1105 22-23 0.39489703589377 1105 24-25 0.36884389537841855 1105 26-27 0.47562569689488043 1105 28-29 0.5887309430374899 1105 30-31 0.39783948877499853 1105 32-33 0.2468348932410933 1105 34-35 0.40945824783749174 1105 36-37 0.28943845573695626 1105 38-39 0.028056965420390156 1105 40-41 0.25111865416993595 1105 42-43 0.22344476584172668 1105 44-45 0.33472222820815745 1105 46-47 0.3154274805350994 1105 48-49 0.32449546827491105 1105 50-51 0.4309703820169588 1105 52-53 0.43053605985340226 1105 54-55 0.5096239739263098 1105 56-57 0.21202741508916745 1105 58-59 0.25791374551722157 1105 60-61 0.23164242026276582 1105 62-63 0.3034033443670765 1105 64-65 0.3192016736990979 1105 66-67 0.39287974210876087 1105 68-69 0.3965500681097964 1105 70-71 0.29470470478669597 1105 72-73 0.21394992989353767 1105 74-75 0.2409137140594737 1105 76-77 0.15317818575327635 1105 78-79 0.27428384803284445 1105 80-81 0.3100484107144936 1105 82-83 0.17730525823731114 1105 84-85 0.19923229426949973 1105 86-87 0.18823311801459397 1105 88-89 0.18935481826411404 1105 90-91 -0.07446525235460655 1105 92-93 0.031039018283479436 1105 94-95 -0.08299104851027295 1105 96-97 0.035258228261803026 1105 98-99 0.09663787856470663 1105 100-101 -0.035301568354690005 1106 1 0.38026429785855953 1106 2 0.414112192296038 1106 3 0.48298944329619786 1106 4 0.33890018181656956 1106 5 0.3633010190726367 1106 6 0.3020117038393124 1106 7 0.27133143192813947 1106 8 0.3445781278658657 1106 9 0.4949402950396333 1106 10-11 0.39901154133035277 1106 12-13 0.39515336824012337 1106 14-15 0.5142636808660868 1106 16-17 0.4791230009852825 1106 18-19 0.4768416402650004 1106 20-21 0.540796566249881 1106 22-23 0.50716770327913 1106 24-25 0.5345198651135021 1106 26-27 0.5196405897898089 1106 28-29 0.3719654562234922 1106 30-31 0.4455252007791515 1106 32-33 0.5603018833191626 1106 34-35 0.6188471600173244 1106 36-37 0.5965270544872325 1106 38-39 0.5525473928424844 1106 40-41 0.4255561778767927 1106 42-43 0.32308342348481034 1106 44-45 0.592712685575755 1106 46-47 0.46544444965130793 1106 48-49 0.3617925875178898 1106 50-51 0.37946412340762947 1106 52-53 0.3147193462721205 1106 54-55 0.3067931260694152 1106 56-57 0.45175610886649764 1106 58-59 0.4345871998303821 1106 60-61 0.46410184411136157 1106 62-63 0.5259508079832855 1106 64-65 0.5138598957349885 1106 66-67 0.5344895123269708 1106 68-69 0.5374710918465908 1106 70-71 0.46474024011239834 1106 72-73 0.4436169359425364 1106 74-75 0.49597949433936606 1106 76-77 0.6159334711338786 1106 78-79 0.5275429165281835 1106 80-81 0.573523885350653 1106 82-83 0.5158166674724995 1106 84-85 0.3148768212304276 1106 86-87 0.3981735264712043 1106 88-89 0.4086831407172511 1106 90-91 0.31191653279642395 1106 92-93 0.4683955294331881 1106 94-95 0.4519478626713749 1106 96-97 0.39811782379793215 1106 98-99 0.3996014848013587 1106 100-101 0.3702927499859321 1107 1 0.1526747159140598 1107 2 0.08186038023411868 1107 3 0.2794553606047785 1107 4 0.2591848710975597 1107 5 0.20349147984950378 1107 6 0.202000528975816 1107 7 0.22196807803048557 1107 8 0.2590005094476311 1107 9 0.16722650512963355 1107 10-11 0.18393652203123168 1107 12-13 0.18317648350947735 1107 14-15 0.2446578966539832 1107 16-17 0.18762476006293127 1107 18-19 0.2703783304245704 1107 20-21 0.20219884463756443 1107 22-23 0.15004154420815752 1107 24-25 0.2472340339412895 1107 26-27 0.20854716150866892 1107 28-29 0.1546792268664774 1107 30-31 0.1557449345605093 1107 32-33 0.19922277684582212 1107 34-35 0.13541614296345728 1107 36-37 0.1547455243529896 1107 38-39 0.17653764116850112 1107 40-41 0.2250033432506413 1107 42-43 0.29363187807826563 1107 44-45 0.4672472268219181 1107 46-47 0.3496677259928447 1107 48-49 0.3362400418968363 1107 50-51 0.20000352250824704 1107 52-53 0.22521703872703114 1107 54-55 0.40186038718743333 1107 56-57 0.360156908832586 1107 58-59 0.40910529989806577 1107 60-61 0.4057016221554548 1107 62-63 0.360392636396476 1107 64-65 0.36218681315532564 1107 66-67 0.27695964525646843 1107 68-69 0.21558428679144725 1107 70-71 0.35763559586482074 1107 72-73 0.20114872971363695 1107 74-75 0.2410461774472097 1107 76-77 0.38080342526556166 1107 78-79 0.20913958616197448 1107 80-81 0.17600316368567093 1107 82-83 0.21455904576603757 1107 84-85 0.15691948351962992 1107 86-87 0.27322763464644595 1107 88-89 0.2707127528210336 1107 90-91 0.3685185008568581 1107 92-93 0.15376403661271354 1107 94-95 0.14893309001563182 1107 96-97 0.13291455046205414 1107 98-99 0.20629754007454437 1107 100-101 0.24921053510229996 1108 1 0.2248100143541123 1108 2 0.17943317077615006 1108 3 0.34910307357064596 1108 4 0.27167896220450416 1108 5 0.15160788855300922 1108 6 0.16104429456710534 1108 7 0.21641956894366388 1108 8 0.10318709473928322 1108 9 0.14643890233829637 1108 10-11 0.12949982090187717 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-1.0110290518073057 2316 66-67 -0.7895691003881353 2316 68-69 -0.710458682062459 2316 70-71 -0.7599731062922572 2316 72-73 -0.5677091578837761 2316 74-75 -0.49843062588816167 2316 76-77 -0.7763941237410137 2316 78-79 -0.5909820159605417 2316 80-81 -0.4155225896989023 2316 82-83 -0.4781782404536088 2316 84-85 -0.5302530302447721 2316 86-87 -0.48885236841230295 2316 88-89 -0.4332143391011911 2316 90-91 -0.3068507939007006 2316 92-93 -0.2790086081461709 2316 94-95 -0.36021906973115847 2316 96-97 -0.41970766673612303 2316 98-99 -0.3617277511720971 2316 100-101 -0.06203437805566736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 142.0 8 343.0 9 377.0 10 475.0 11 513.0 12 683.0 13 694.0 14 697.0 15 822.0 16 1084.0 17 1396.0 18 1792.0 19 2415.0 20 3309.0 21 4168.0 22 4538.0 23 4359.0 24 4098.0 25 4084.0 26 4412.0 27 5223.0 28 6182.0 29 7201.0 30 9059.0 31 11462.0 32 15122.0 33 20688.0 34 30726.0 35 45460.0 36 77635.0 37 181206.0 38 336669.0 39 113019.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.65713552378508 23.044076813589378 12.759820310512145 23.538967352113396 2 13.030067334829662 23.19295984355208 42.52116713704749 21.25580568457077 3 15.75624183384145 33.21571102242527 30.256895126525464 20.771152017207818 4 10.101003367519601 18.536467759693977 44.60902441923897 26.75350445354745 5 10.108827891938736 42.004455332788915 36.74805148631457 11.138665288957775 6 25.010582968808855 43.226399979556255 20.310411530269512 11.452605521365374 7 25.25376988271476 33.19374508348629 27.363815347966952 14.188669685831998 8 24.331585262877205 37.08568508066308 22.941758144082485 15.640971512377227 9 27.402727561616803 16.307480683290407 22.375049722332715 33.91474203276007 10-11 22.029452009513264 28.912402299316724 31.322996000584443 17.73514969058557 12-13 23.739725479477624 26.920354827885813 30.235089029178635 19.10483066345793 14-15 22.123705521663243 27.711585615833386 27.47849341108999 22.686215451413386 16-17 19.823836511038547 31.1199202731334 28.608401762968118 20.447841452859937 18-19 19.9696578480737 29.67664665336397 30.38959278632281 19.96410271223952 20-21 20.694461341381647 29.57232509447934 30.028347968395597 19.704865595743414 22-23 20.975567483009673 29.32335337990003 29.38490407978926 20.316175057301038 24-25 20.449135476703006 30.460940212485244 28.982042913686552 20.107881397125198 26-27 19.630749539325773 30.6225448994183 29.831246295301483 19.91545926595444 28-29 19.992744819295446 28.41225425575234 31.112059210274133 20.482941714678073 30-31 20.73473111212472 29.8392011782543 29.34518773011043 20.080879979510556 32-33 20.432544616023883 29.17898516669278 30.663038055971263 19.725432161312074 34-35 20.53122357853196 29.783473118291514 30.44036428156383 19.24493902161269 36-37 20.910526146212586 28.836272169035176 29.12191566684036 21.13128601791188 38-39 20.316019689568762 30.29779333952174 29.792832894756575 19.593354076152924 40-41 19.987545454646458 29.06941992482832 31.32829226073885 19.614742359786373 42-43 20.168586794034468 28.809854339187872 29.80833168068501 21.213227186092645 44-45 19.85837744571663 29.532918883396096 29.875948330599282 20.73275534028799 46-47 20.901116604633078 28.504305316371315 29.60479973334815 20.989778345647466 48-49 19.846163760155015 29.60803201607025 29.23127433203562 21.314529891739106 50-51 19.928625751282297 29.53453968109355 29.01150998645067 21.525324581173486 52-53 19.751675697415656 28.958984437928926 30.34220557665878 20.94713428799664 54-55 20.25619033122869 29.637429408503536 29.478108997825732 20.62827126244205 56-57 20.521891002174268 29.82074787267255 28.81788477156853 20.839476353584654 58-59 19.71617857662656 28.33131312682416 30.464584539254037 21.487923757295242 60-61 20.702765220154365 29.947459817148165 29.070642047922778 20.279132914774692 62-63 19.935282272064438 29.543059509756265 30.379945837094645 20.14171238108465 64-65 20.172779386022658 29.15839628820415 30.012127110786686 20.656697214986508 66-67 20.249809592959465 30.095756270099677 28.826209341638844 20.828224795302017 68-69 20.4578118741309 29.130715217858693 29.643882739804567 20.767590168205835 70-71 20.763419673058216 28.17879972468762 30.164481925542336 20.893298676711826 72-73 20.039763441409754 29.466109971080122 29.414669699013302 21.07945688849682 74-75 20.519502307035093 29.68570327073471 28.258152394305796 21.5366420279244 76-77 19.541359423069018 29.862800017331924 30.514247177728915 20.081593381870135 78-79 19.903107859029213 28.910210616250016 29.52099440823133 21.66568711648944 80-81 19.736724406246474 30.364977778864283 28.567229839159978 21.331067975729265 82-83 20.80684383311101 29.781318909044646 28.55254685328905 20.859290404555296 84-85 20.395617779131 29.256124785950576 28.57983418323408 21.76842325168434 86-87 18.981825131900028 29.91730412790413 30.445348718336213 20.655522021859625 88-89 19.9815431217015 28.8206765929336 29.932422934754772 21.265357350610127 90-91 20.1298647182329 29.30973463127676 29.831660344924337 20.728740305566003 92-93 20.561787140896794 29.677439068428917 29.600048889703718 20.16072490097057 94-95 20.1801640613149 28.221646691963574 29.727514637218682 21.870674609502842 96-97 20.57743280849038 28.381742554169048 29.95754922173573 21.08327541560484 98-99 19.918083063684527 28.437466664296128 30.086806172883406 21.55764409913594 100-101 19.967764266895426 28.97271241303519 30.13718799066153 20.922335329407858 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 52.0 1 39.0 2 54.5 3 92.0 4 80.0 5 68.5 6 113.5 7 220.5 8 357.0 9 392.5 10 320.5 11 278.5 12 282.0 13 275.5 14 297.0 15 337.5 16 349.0 17 368.5 18 415.0 19 463.5 20 500.0 21 537.5 22 609.0 23 694.0 24 761.0 25 873.0 26 1181.0 27 1607.5 28 1858.5 29 2272.5 30 3603.5 31 5784.5 32 11489.0 33 20199.5 34 29898.5 35 43662.5 36 67074.5 37 86258.0 38 82705.5 39 72963.5 40 59837.5 41 44891.5 42 37042.5 43 33787.0 44 32599.5 45 31614.5 46 28490.5 47 23154.5 48 21341.5 49 19690.0 50 18027.0 51 15369.0 52 12132.0 53 10583.5 54 9817.0 55 9257.0 56 8883.0 57 7797.0 58 6604.0 59 6487.5 60 5944.5 61 4651.0 62 3741.0 63 2749.5 64 1696.5 65 1316.5 66 887.5 67 563.5 68 455.5 69 278.5 70 204.5 71 176.0 72 132.5 73 93.0 74 80.0 75 78.5 76 80.0 77 63.0 78 28.5 79 18.5 80 12.5 81 12.5 82 13.5 83 6.5 84 2.0 85 2.5 86 3.0 87 3.5 88 2.5 89 1.0 90 0.5 91 0.5 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008443759561724438 2 0.010332495253162799 3 0.001888735691438361 4 4.444083979854967E-4 5 0.003110858785898477 6 0.0046662881788477155 7 0.004999594477336838 8 0.0036663692833803483 9 0.008332657462228063 10-11 0.007388289616508883 12-13 0.0034441650843875997 14-15 0.0 16-17 0.0 18-19 5.555104974818709E-4 20-21 9.443678457191806E-4 22-23 2.2220419899274835E-4 24-25 0.0011665720447119289 26-27 0.0037219203331285353 28-29 0.002944205636653916 30-31 0.010832454700896483 32-33 4.444083979854967E-4 34-35 0.0 36-37 0.0 38-39 2.7775524874093543E-4 40-41 0.0 42-43 2.7775524874093543E-4 44-45 4.999594477336838E-4 46-47 0.002555348288416606 48-49 0.0027775524874093546 50-51 0.003166409835646664 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0011665720447119289 64-65 0.0026108993381647933 66-67 0.00305530773615029 68-69 0.008277106412479878 70-71 4.999594477336838E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015720947078736947 82-83 0.012165679894852973 84-85 0.018220744317405363 86-87 0.016554212824959755 88-89 0.015276538680751451 90-91 0.015165436581255074 92-93 0.009776984755680928 94-95 0.025164625535928756 96-97 0.009943637904925489 98-99 0.015220987631003264 100-101 0.015554293929492384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 900073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.60974209444827 #Duplication Level Percentage of deduplicated Percentage of total 1 64.46247948161576 10.707051589583012 2 12.198982085276434 4.052438925024726 3 5.601883613070755 2.7913752616866385 4 3.3536024272851614 2.2280988561808894 5 2.1818923459536474 1.8120334572070393 6 1.5983916155051279 1.5929323499681218 7 1.228135241584469 1.4279306733874664 8 0.9915357407592701 1.3175322345151357 9 0.7518820632076193 1.1239710439788184 >10 5.8980347825209325 19.85340572547774 >50 0.8590903068679172 10.054058588935684 >100 0.7643176460309009 25.824204915038262 >500 0.07840903594429403 8.72044823126882 >1k 0.03136361437771761 8.494518147747641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 4687 0.5207355403395059 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3437 0.38185791596903806 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 2905 0.32275159903696704 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2382 0.2646452010003633 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2374 0.2637563842043923 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2214 0.24598004828497244 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2142 0.2379806971212335 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2119 0.23542534883281688 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 2039 0.22653718087310695 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1975 0.21942664650533902 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1936 0.21509366462498042 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1906 0.2117606016400892 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1853 0.20587219036678137 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1807 0.20076149378994818 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1700 0.1888735691438361 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1674 0.1859849145569304 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 1606 0.17842997179117692 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1594 0.17709674659722044 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1508 0.16754196604053226 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1507 0.16743086394103587 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1504 0.16709755764254677 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1497 0.16631984294607216 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1396 0.15509853089693837 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1376 0.15287648890701086 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1350 0.14998783432010515 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1343 0.1492101196236305 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1319 0.14654366923571754 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1295 0.14387721884780458 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1288 0.14309950415132996 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1280 0.14221068735535894 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1274 0.1415440747583807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.13709999077852575 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1220 0.13554456138557652 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 1207 0.13410023409212363 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1197 0.13298921309715991 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1188 0.13198929420169253 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1166 0.1295450480127723 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 1153 0.12810072071931944 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 1150 0.1277674144208303 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1065 0.11832373596363852 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 1054 0.1171016128691784 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1024 0.11376854988428715 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1022 0.11354634568529441 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 1020 0.11332414148630167 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 1012 0.11243532469033066 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1009 0.11210201839184156 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 998 0.11087989529738143 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 967 0.10743573021299384 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 939 0.10432487142709537 No Hit CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC 925 0.10276944203414613 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 923 0.10254723783515336 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 918 0.1019917273376715 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 916 0.10176952313867875 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 909 0.10099180844220412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1110209949637419E-4 0.0 5 0.0 0.0 0.0 1.1110209949637419E-4 0.0 6 0.0 0.0 0.0 1.1110209949637419E-4 0.0 7 0.0 0.0 0.0 1.1110209949637419E-4 0.0 8 0.0 0.0 0.0 1.1110209949637419E-4 0.0 9 0.0 0.0 0.0 2.2220419899274837E-4 0.0 10-11 0.0 0.0 0.0 4.4440839798549675E-4 0.0 12-13 0.0 0.0 0.0 7.221636467264322E-4 0.0 14-15 0.0 0.0 0.0 8.332657462228063E-4 0.0 16-17 0.0 0.0 0.0 0.0010554699452155548 0.0 18-19 0.0 0.0 0.0 0.0012776741442083032 0.0 20-21 0.0 0.0 0.0 0.0019442867411865483 0.0 22-23 0.0 0.0 0.0 0.003555267183883974 0.0 24-25 0.0 0.0 0.0 0.006610574920034263 0.0 26-27 0.0 0.0 0.0 0.008499310611472626 0.0 28-29 0.0 0.0 0.0 0.010943556800392856 0.0 30-31 0.0 0.0 0.0 0.014554375034025017 0.0 32-33 0.0 0.0 0.0 0.021831562551037527 0.0 34-35 0.0 0.0 0.0 0.03277511935143038 0.0 36-37 0.0 0.0 0.0 0.05071810842009482 0.0 38-39 0.0 0.0 0.0 0.08671518865692004 0.0 40-41 0.0 0.0 0.0 0.13104492635597334 0.0 42-43 0.0 0.0 0.0 0.17759670604495414 0.0 44-45 0.0 0.0 0.0 0.22520395567915047 0.0 46-47 0.0 0.0 0.0 0.28653231460114903 0.0 48-49 0.0 0.0 0.0 0.35347132954771443 0.0 50-51 0.0 0.0 0.0 0.4309650439464355 0.0 52-53 0.0 0.0 0.0 0.5145138227677089 0.0 54-55 0.0 0.0 0.0 0.601562317723118 0.0 56-57 0.0 0.0 0.0 0.6983322463844599 0.0 58-59 0.0 0.0 0.0 0.8148227977064083 0.0 60-61 0.0 0.0 0.0 0.9432568247242168 0.0 62-63 0.0 0.0 0.0 1.0720797090902625 0.0 64-65 0.0 0.0 0.0 1.2245673406490363 0.0 66-67 0.0 0.0 0.0 1.3869986101127352 0.0 68-69 0.0 0.0 0.0 1.5717058505254573 0.0 70-71 0.0 0.0 0.0 1.7914102522795372 0.0 72-73 0.0 0.0 0.0 2.025169069619909 0.0 74-75 0.0 0.0 0.0 2.2710380158053844 0.0 76-77 0.0 0.0 0.0 2.5346832979102807 0.0 78-79 0.0 0.0 0.0 2.8413806435700213 0.0 80-81 0.0 0.0 0.0 3.176409024601338 0.0 82-83 0.0 0.0 0.0 3.5463790159242636 0.0 84-85 0.0 0.0 0.0 3.9315699948781933 0.0 86-87 0.0 0.0 0.0 4.358257607994018 0.0 88-89 0.0 0.0 0.0 4.815164992172857 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 375 0.0 62.0654 1 GTCTAAT 60 1.4921316E-6 47.49903 2 CATATAG 505 0.0 46.090725 3 ACATATA 545 0.0 43.57709 2 CCGAAGA 45 9.624931E-4 42.22136 9 GGGTCAC 70 4.309757E-6 40.71345 8 ATGCCCC 60 8.290318E-5 39.582527 9 AACGTTC 30 8.296341E-5 39.580326 20-21 CGACTTA 25 0.001616663 37.997112 22-23 CATTGCG 25 0.0016171043 37.995 16-17 GTCTTCG 65 1.3281159E-4 36.537716 1 CATGGGG 1940 0.0 36.23637 4 GTATCAA 3370 0.0 36.223293 1 GTACATG 5630 0.0 35.94065 1 ATTGAAC 220 0.0 34.544746 8 GGTATCA 1900 0.0 34.2493 1 ACATGGG 5425 0.0 33.885983 3 CCCTATA 290 0.0 32.757954 2 ATGGGGT 650 0.0 32.153187 5 TATAAGC 565 0.0 31.946247 2 >>END_MODULE