##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568665_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 900073 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73045186334886 34.0 31.0 34.0 31.0 34.0 2 32.83646215362532 34.0 31.0 34.0 31.0 34.0 3 32.910920558665794 34.0 31.0 34.0 31.0 34.0 4 36.30267211659499 37.0 37.0 37.0 35.0 37.0 5 36.3542679316011 37.0 37.0 37.0 35.0 37.0 6 36.38009250360804 37.0 37.0 37.0 35.0 37.0 7 36.31434561418907 37.0 37.0 37.0 35.0 37.0 8 36.31815308313881 37.0 37.0 37.0 35.0 37.0 9 38.142652873711356 39.0 39.0 39.0 37.0 39.0 10-11 38.060682855723925 39.0 39.0 39.0 36.0 39.0 12-13 38.07782257661323 39.0 39.0 39.0 37.0 39.0 14-15 39.518040203405725 41.0 39.0 41.0 37.0 41.0 16-17 39.50813045164115 41.0 39.0 41.0 37.0 41.0 18-19 39.61810486482763 41.0 40.0 41.0 37.0 41.0 20-21 39.59920084259832 41.0 40.0 41.0 37.0 41.0 22-23 39.562693248214316 41.0 40.0 41.0 37.0 41.0 24-25 39.53025865679784 41.0 39.0 41.0 37.0 41.0 26-27 39.43503971344546 41.0 39.0 41.0 36.0 41.0 28-29 39.349554425029964 41.0 39.0 41.0 36.0 41.0 30-31 39.28481134307995 41.0 39.0 41.0 36.0 41.0 32-33 39.12754298817985 41.0 39.0 41.0 35.0 41.0 34-35 39.048953807080096 41.0 39.0 41.0 35.0 41.0 36-37 38.963870152754275 41.0 39.0 41.0 35.0 41.0 38-39 38.8210239613898 40.0 38.0 41.0 35.0 41.0 40-41 38.78486967168219 40.0 38.0 41.0 35.0 41.0 42-43 38.67821332269716 40.0 38.0 41.0 35.0 41.0 44-45 38.56530970265745 40.0 38.0 41.0 35.0 41.0 46-47 38.38043803113747 40.0 38.0 41.0 34.0 41.0 48-49 38.21708572526895 40.0 38.0 41.0 34.0 41.0 50-51 38.22390072805206 40.0 38.0 41.0 34.0 41.0 52-53 38.33522614276842 40.0 38.0 41.0 34.0 41.0 54-55 38.236062519373434 40.0 38.0 41.0 34.0 41.0 56-57 38.13356305544106 40.0 38.0 41.0 34.0 41.0 58-59 37.958010072516345 40.0 38.0 41.0 33.5 41.0 60-61 37.915579069697685 40.0 37.5 41.0 33.5 41.0 62-63 37.73772071820841 40.0 37.0 41.0 33.0 41.0 64-65 37.50491737892371 39.5 37.0 41.0 33.0 41.0 66-67 37.27111134319105 39.0 36.0 41.0 33.0 41.0 68-69 36.95062289392082 39.0 36.0 41.0 32.5 41.0 70-71 36.55536939781551 38.0 35.0 40.5 32.0 41.0 72-73 36.16809192143304 37.0 35.0 39.5 32.0 41.0 74-75 35.75392495941996 37.0 35.0 39.0 31.0 41.0 76-77 33.926028777665806 35.5 33.0 37.5 29.0 39.0 78-79 34.84617358814229 36.0 34.5 37.5 31.0 39.0 80-81 34.68512276226484 35.5 35.0 37.0 31.0 39.0 82-83 34.40863963256314 35.0 35.0 37.0 31.0 39.0 84-85 34.03253569432702 35.0 34.5 36.0 30.5 38.0 86-87 33.82409871199336 35.0 34.0 36.0 31.0 37.0 88-89 33.63060496204197 35.0 34.0 36.0 30.0 37.0 90-91 33.495298159149314 35.0 34.0 35.5 30.0 36.5 92-93 33.37255200411522 35.0 34.0 35.0 30.0 36.0 94-95 33.25487988196513 35.0 34.0 35.0 30.0 36.0 96-97 33.152318756367535 35.0 34.0 35.0 30.0 36.0 98-99 33.08659130981598 35.0 34.0 35.0 30.0 36.0 100-101 32.022011547952225 34.5 32.5 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30523989553748976 1101 2 0.24918492474129295 1101 3 0.20500451771553685 1101 4 0.18128001731680143 1101 5 0.209352950184865 1101 6 0.11851462041566663 1101 7 0.24624342637714136 1101 8 0.23637727086489946 1101 9 0.32594244559737007 1101 10-11 0.28854480839874697 1101 12-13 0.2417714701239717 1101 14-15 0.37388398936454337 1101 16-17 0.30989710295663997 1101 18-19 0.35431732463205634 1101 20-21 0.4116181639184475 1101 22-23 0.3752797757248558 1101 24-25 0.32776920756121797 1101 26-27 0.2890010179904081 1101 28-29 0.37486509213992036 1101 30-31 0.1683127275427978 1101 32-33 0.25821182361034545 1101 34-35 0.227887313871463 1101 36-37 0.3133515062242296 1101 38-39 0.27567841625548795 1101 40-41 0.33239321594086846 1101 42-43 0.2901290479386134 1101 44-45 0.302439346082636 1101 46-47 0.2769047697638669 1101 48-49 -0.007700578832476879 1101 50-51 0.1892598033023063 1101 52-53 0.27144170815250845 1101 54-55 0.317613307134998 1101 56-57 0.44517573850765046 1101 58-59 0.4329418553639357 1101 60-61 0.555800992999032 1101 62-63 0.5768347023415075 1101 64-65 0.38841809666170235 1101 66-67 0.48727979533364163 1101 68-69 0.3924363775387718 1101 70-71 0.3242136870454715 1101 72-73 0.2068842973178704 1101 74-75 0.2337704003596528 1101 76-77 0.0618683427912643 1101 78-79 0.21223869766536296 1101 80-81 0.3780071592049481 1101 82-83 0.3754720913083389 1101 84-85 0.3579249893469907 1101 86-87 0.3119904595423435 1101 88-89 0.4006742134302854 1101 90-91 0.3824695540907257 1101 92-93 0.3422916226002286 1101 94-95 0.44809215657083 1101 96-97 0.46405330615596085 1101 98-99 0.3087292318488011 1101 100-101 0.0013538894457951756 1102 1 0.2378205909593376 1102 2 0.06244286444370317 1102 3 0.06035326735847946 1102 4 0.10058784146180244 1102 5 0.15944903162036894 1102 6 0.14950412936162394 1102 7 0.19244431448122157 1102 8 0.16386175501146027 1102 9 0.043768148679767194 1102 10-11 0.1334950148439944 1102 12-13 0.19263036025355262 1102 14-15 0.23832963843359067 1102 16-17 0.2912180279352157 1102 18-19 0.22452299083996508 1102 20-21 0.18792370288336002 1102 22-23 0.23722276307846357 1102 24-25 0.11825649624698542 1102 26-27 0.1491311174476877 1102 28-29 0.034113579506517056 1102 30-31 0.11582662039901948 1102 32-33 0.17742875991095275 1102 34-35 0.23796774961375178 1102 36-37 0.09080125823025753 1102 38-39 0.2543798237186792 1102 40-41 0.015462433526373331 1102 42-43 0.08659870438255979 1102 44-45 0.12955443606160344 1102 46-47 0.14038473548615826 1102 48-49 -0.061327653022921425 1102 50-51 -0.029211852674215777 1102 52-53 0.16177740889778391 1102 54-55 -0.039622988276462934 1102 56-57 -0.03817880198936763 1102 58-59 -0.016888054527257168 1102 60-61 -0.09569605963801564 1102 62-63 0.029569777259048635 1102 64-65 -0.07150198695511278 1102 66-67 -0.23121458260398242 1102 68-69 -0.02885312621359759 1102 70-71 -0.1323240703618893 1102 72-73 -0.13227505504131898 1102 74-75 0.020253754900153353 1102 76-77 0.02011998526445069 1102 78-79 0.027415020083502384 1102 80-81 0.03078800264414383 1102 82-83 0.06678126578702859 1102 84-85 0.07340383844721288 1102 86-87 -0.0010873274684897183 1102 88-89 0.04173863992799198 1102 90-91 -0.09408787404294827 1102 92-93 -0.1274654471756662 1102 94-95 -0.11802875023017378 1102 96-97 -0.04960096946792447 1102 98-99 -0.18051029657312512 1102 100-101 -0.17193352388615324 1103 1 0.28482249548719807 1103 2 0.3167987039718696 1103 3 0.29284082649100185 1103 4 0.20302379403291582 1103 5 0.22158578900809545 1103 6 0.181973257579358 1103 7 0.15088260163487632 1103 8 0.12679718537332008 1103 9 0.20141390649754243 1103 10-11 0.1874730206190165 1103 12-13 0.2138044095255367 1103 14-15 0.21184287797405688 1103 16-17 0.23892337904414518 1103 18-19 0.15509680665368109 1103 20-21 0.18695761576657333 1103 22-23 0.15324997939595875 1103 24-25 0.2235323456049727 1103 26-27 0.2836829390364244 1103 28-29 0.21101042782008506 1103 30-31 0.2537697303087043 1103 32-33 0.23211174817659952 1103 34-35 0.270988269365553 1103 36-37 0.2704642796715575 1103 38-39 0.2566345389610447 1103 40-41 0.2175359114399953 1103 42-43 0.216989746957978 1103 44-45 0.1823236810084623 1103 46-47 0.19821480245182244 1103 48-49 0.3265463409563125 1103 50-51 0.19877702734053315 1103 52-53 0.1689869683989258 1103 54-55 0.21060739815838758 1103 56-57 0.18748778276211908 1103 58-59 0.061650681111984795 1103 60-61 0.21686460878982672 1103 62-63 0.22783419944988026 1103 64-65 0.1896533242201599 1103 66-67 0.1230982514563479 1103 68-69 0.15177444643492066 1103 70-71 0.23819721734469113 1103 72-73 0.2321640307853059 1103 74-75 0.062400649291014076 1103 76-77 0.013323585436474161 1103 78-79 0.3122386976653573 1103 80-81 0.4300092964943616 1103 82-83 0.2487496874863666 1103 84-85 0.379467609412373 1103 86-87 0.3445998384711828 1103 88-89 0.4965567882354165 1103 90-91 0.3211544924865066 1103 92-93 0.2591322059545149 1103 94-95 0.3426043516927777 1103 96-97 0.34155833507222155 1103 98-99 0.30452380061922923 1103 100-101 0.32676248889261217 1104 1 0.2816540701859225 1104 2 0.09925560300894176 1104 3 0.1292416798612308 1104 4 0.22942349420013386 1104 5 0.19597891591809002 1104 6 0.18644191928725462 1104 7 0.20643815719043346 1104 8 0.2208524237980427 1104 9 0.09877883759366313 1104 10-11 0.21508358395869465 1104 12-13 0.19289683874769992 1104 14-15 0.26263426354748276 1104 16-17 0.3340510962787633 1104 18-19 0.2128693950178686 1104 20-21 0.32257573258603855 1104 22-23 0.26179088927315064 1104 24-25 0.18994963211826388 1104 26-27 0.2716665635535236 1104 28-29 0.38944553132005666 1104 30-31 0.3028558169142528 1104 32-33 0.21842178680890356 1104 34-35 0.31970965551558805 1104 36-37 0.2659163352559446 1104 38-39 0.2977577587038809 1104 40-41 0.21983655146766523 1104 42-43 0.38014635192828194 1104 44-45 0.335277322247002 1104 46-47 0.31632008988767524 1104 48-49 0.19235242986826506 1104 50-51 0.15566020787446178 1104 52-53 0.26263281975105457 1104 54-55 0.17772669701094657 1104 56-57 0.32363348996387487 1104 58-59 0.35935724860319596 1104 60-61 0.5255799406239987 1104 62-63 0.42159537110414647 1104 64-65 0.2574515713515737 1104 66-67 0.27478192786757916 1104 68-69 0.29706793660386666 1104 70-71 0.23369828405748194 1104 72-73 0.3058810924240092 1104 74-75 0.28103496861292143 1104 76-77 0.31368251987688467 1104 78-79 0.25922451326820095 1104 80-81 0.35197348954054775 1104 82-83 0.2705971187266272 1104 84-85 0.2884683589943364 1104 86-87 0.27128495638618233 1104 88-89 0.34895112600857914 1104 90-91 0.2699753423971387 1104 92-93 0.3087285848970396 1104 94-95 0.19927418966775434 1104 96-97 0.316830779286029 1104 98-99 0.3176645490479899 1104 100-101 0.2108814417842737 1105 1 0.2823352418011069 1105 2 0.12007140619041934 1105 3 0.2075163122289041 1105 4 0.1830637554494814 1105 5 0.2307286855218038 1105 6 0.13191813838410127 1105 7 0.19043062423374835 1105 8 0.13321168172167575 1105 9 0.1468114536933527 1105 10-11 0.25091521436298336 1105 12-13 0.20734168388133156 1105 14-15 0.3367567542314589 1105 16-17 0.36188396881953366 1105 18-19 0.2852538963601745 1105 20-21 0.3250449796910644 1105 22-23 0.3273336227747663 1105 24-25 0.3168629229785438 1105 26-27 0.23900538632869228 1105 28-29 0.12854177686238444 1105 30-31 0.1840715079027575 1105 32-33 0.36238941112272727 1105 34-35 0.37711338993879195 1105 36-37 0.4269683447122006 1105 38-39 0.3639516121317783 1105 40-41 0.4494464805456886 1105 42-43 0.4946182435819253 1105 44-45 0.5268117614824845 1105 46-47 0.6253816758154684 1105 48-49 0.6408738867741377 1105 50-51 0.5967238373171071 1105 52-53 0.8245643419692712 1105 54-55 0.815643914625241 1105 56-57 0.8159602920634796 1105 58-59 0.7872432124610285 1105 60-61 0.8248396673539773 1105 62-63 0.890466830013473 1105 64-65 0.6982898131274311 1105 66-67 0.6936473764391238 1105 68-69 0.5249899624883909 1105 70-71 0.5850726479634005 1105 72-73 0.6494370085998327 1105 74-75 0.5139956610038396 1105 76-77 0.5425712084211796 1105 78-79 0.5985381326936121 1105 80-81 0.45820620981942994 1105 82-83 0.4489839440662138 1105 84-85 0.5336049940065593 1105 86-87 0.5775817869347293 1105 88-89 0.5454991886763736 1105 90-91 0.45491844453280805 1105 92-93 0.43246140534820654 1105 94-95 0.5229509136329753 1105 96-97 0.5279438938430729 1105 98-99 0.5672838943218679 1105 100-101 0.5015213424133549 1106 1 0.26730260120698546 1106 2 0.049869174252542336 1106 3 0.05827792609167659 1106 4 0.06728447374219115 1106 5 0.08911142892880974 1106 6 0.15255557824355037 1106 7 0.17620651211779403 1106 8 0.1658273212577157 1106 9 0.2426819693548552 1106 10-11 0.188623585128191 1106 12-13 0.2553942282505801 1106 14-15 0.30854559853482755 1106 16-17 0.4138968054568508 1106 18-19 0.24800668628251543 1106 20-21 0.3044150262064065 1106 22-23 0.2616781157974728 1106 24-25 0.22962918837551882 1106 26-27 0.18828519707313518 1106 28-29 0.25294785779508544 1106 30-31 0.20340631854737978 1106 32-33 0.07562132903213126 1106 34-35 0.15036357460270722 1106 36-37 0.3166915484813515 1106 38-39 0.20170190659585074 1106 40-41 0.08180808243025695 1106 42-43 0.12411505937718204 1106 44-45 0.05978425155056044 1106 46-47 0.011528887889497241 1106 48-49 0.2089050700206343 1106 50-51 0.19475292437865477 1106 52-53 0.26912773659105227 1106 54-55 0.1330412514721786 1106 56-57 0.20455305323815054 1106 58-59 0.23551735352430114 1106 60-61 0.2269453350387849 1106 62-63 0.340669960316319 1106 64-65 0.27397706233387 1106 66-67 0.20957588655071646 1106 68-69 0.275319362979495 1106 70-71 0.29134223731078635 1106 72-73 0.16903883327891123 1106 74-75 0.010347301019010047 1106 76-77 -0.04903456906379944 1106 78-79 0.11432939260058106 1106 80-81 0.23090489820656046 1106 82-83 0.18025303432195017 1106 84-85 0.15818575701788262 1106 86-87 0.008652238712500093 1106 88-89 0.06193427681595409 1106 90-91 0.0014512548180860563 1106 92-93 -0.0019080422571420286 1106 94-95 0.029658705451872436 1106 96-97 0.06414301144902623 1106 98-99 0.16942799206397297 1106 100-101 0.188136702458074 1107 1 0.071878643584121 1107 2 0.005120910530905576 1107 3 0.036684750208316075 1107 4 0.05364086454386552 1107 5 0.03245101761676494 1107 6 0.0284503701149319 1107 7 0.11216940643313222 1107 8 0.01625113116686805 1107 9 -0.003243379988077777 1107 10-11 0.021705805149693447 1107 12-13 0.11466352062482343 1107 14-15 0.13267765202517268 1107 16-17 0.09396367481242152 1107 18-19 0.07257160517484351 1107 20-21 0.07822487113269716 1107 22-23 0.13209337115594622 1107 24-25 0.06811901475410309 1107 26-27 0.12094123092141729 1107 28-29 0.10320661368597683 1107 30-31 0.10793894593761166 1107 32-33 0.19575046935116092 1107 34-35 0.018541930561930542 1107 36-37 0.10331331017930978 1107 38-39 0.1455764758177125 1107 40-41 0.3169946448495722 1107 42-43 0.2904297820671573 1107 44-45 0.12755401853539183 1107 46-47 0.05223108034633128 1107 48-49 0.17355075620221072 1107 50-51 0.22162525991227966 1107 52-53 0.2719047543924873 1107 54-55 0.20768163280476415 1107 56-57 0.3755965414396698 1107 58-59 0.3402932589772405 1107 60-61 0.3309614782208996 1107 62-63 0.3821177859062388 1107 64-65 0.3098470896801757 1107 66-67 0.312384630688733 1107 68-69 0.23182538134994246 1107 70-71 0.21233145677868492 1107 72-73 0.2467336772996589 1107 74-75 0.24785361096348169 1107 76-77 -0.017187492575068575 1107 78-79 0.23772942830876076 1107 80-81 0.20061266910387587 1107 82-83 0.17286863314691914 1107 84-85 0.16385732135747588 1107 86-87 0.20449557483312475 1107 88-89 0.3002093403007464 1107 90-91 0.27147898646209256 1107 92-93 0.2740629185113761 1107 94-95 0.2847879035719174 1107 96-97 0.3432699078040926 1107 98-99 0.2740253565944428 1107 100-101 0.24831141566868808 1108 1 0.14937289850195867 1108 2 -0.023026481833639423 1108 3 0.03362382659567942 1108 4 0.09908785767784423 1108 5 0.10841715088394466 1108 6 0.08909667430708623 1108 7 0.10581822395247542 1108 8 0.08115183836191164 1108 9 0.024008902833777768 1108 10-11 0.06256199790363581 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-0.6234762131171365 2316 74-75 -0.5228889848553351 2316 76-77 -0.6276825121245011 2316 78-79 -0.6238893511151247 2316 80-81 -0.48614924009100235 2316 82-83 -0.5187045808063218 2316 84-85 -0.6680171466851874 2316 86-87 -0.5765129664068667 2316 88-89 -0.6766139434718994 2316 90-91 -0.7363150197086128 2316 92-93 -0.659998891606449 2316 94-95 -0.4558712580633184 2316 96-97 -0.5146916649277742 2316 98-99 -0.6671987603563778 2316 100-101 -0.594655194034214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 0.0 12 0.0 13 2.0 14 27.0 15 108.0 16 300.0 17 752.0 18 1223.0 19 1837.0 20 2672.0 21 3697.0 22 4603.0 23 4090.0 24 3598.0 25 3516.0 26 4135.0 27 4999.0 28 6358.0 29 7666.0 30 9284.0 31 11575.0 32 14662.0 33 19270.0 34 27020.0 35 40259.0 36 72458.0 37 176241.0 38 351574.0 39 128131.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.863559888963216 24.066933220133308 13.058378765215883 23.01112812568759 2 12.767457741732416 23.699886777427555 42.80491684035617 20.727738640483853 3 15.393529191521132 33.644382177890016 30.817944766702254 20.144143863886598 4 9.978190657868861 19.012013470018545 44.933688711915586 26.076107160197004 5 9.981079312455767 42.29934683075706 36.979778306870664 10.739795549916508 6 24.675536790644703 43.79945001527735 20.393100191661343 11.1319130024166 7 24.52036723593942 33.71682468273611 27.671723699520896 14.091084381803572 8 23.900616949958504 37.46696101316226 23.091015950928426 15.541406085950808 9 26.99092184745015 16.190686755407615 22.831148140206405 33.98724325693583 10-11 21.650299475709193 29.000869929439055 31.81791921321937 17.53091138163238 12-13 23.416378449303558 27.245123451097857 30.54791111387632 18.790586985722268 14-15 21.99543814779468 27.788968228132603 27.881682930162334 22.333910693910383 16-17 19.594521777678032 31.328458913888095 28.88126851933121 20.19575078910266 18-19 19.563857598217034 29.58815562737689 30.90971510088626 19.938271673519814 20-21 20.437397855507275 29.436279057365343 30.522746488340392 19.603576598786987 22-23 20.65760221670909 29.370506614463494 29.71864504323538 20.253246125592035 24-25 20.23346995188168 30.308041681063646 29.58410040074527 19.8743879663094 26-27 19.584578139773107 30.323540423943392 30.336539369584465 19.755342066699033 28-29 19.807913232643973 28.152671704838113 31.647319851299134 20.392095211218784 30-31 20.75903843354928 29.539048499399495 29.841301761079382 19.860611305971847 32-33 20.30640848020105 28.959317744227413 31.060369547803347 19.67390422776819 34-35 20.30674178649954 29.59065542461556 31.187081492278963 18.91552129660594 36-37 20.890261617814122 28.592085637545868 29.41151962758556 21.10613311705446 38-39 20.10786902840103 30.051062524928536 30.42469888553484 19.416369561135596 40-41 19.875887844652603 29.015979814970567 31.66165411027772 19.446478230099114 42-43 20.038763522514287 28.712726634395207 30.150332250828544 21.098177592261962 44-45 19.6965135050157 29.359396404513856 30.243769116504993 20.700320973965443 46-47 20.74365079276903 28.511520732207273 29.85780042285459 20.8870280521691 48-49 19.590799857344905 29.565101941731392 29.61781988794242 21.226278312981282 50-51 19.811948586392436 29.31384454372034 29.304178661064157 21.57002820882306 52-53 19.571079234684298 28.78277650812767 30.572242473666023 21.073901783522004 54-55 20.12281226078329 29.55438058912999 29.702368585659162 20.620438564427552 56-57 20.533612273671135 29.6062652695948 28.973705466112193 20.88641699062187 58-59 19.603632149836734 28.37808711071213 30.590296564834183 21.42798417461695 60-61 20.709776156920693 30.072410833571737 29.029883703812747 20.18792930569482 62-63 19.717432774598393 29.72949851622487 30.659538324284025 19.893530384892713 64-65 20.072327707844945 29.242503360849714 29.978668325796882 20.70650060550846 66-67 20.095814450605673 30.327206793226775 28.77399944226746 20.802979313900096 68-69 20.4059559613498 29.35228587014609 29.569546025711247 20.672212142792862 70-71 20.555721591470913 28.277595261717657 30.325540261734325 20.841142885077097 72-73 19.94338237009665 29.433001545430205 29.397226669392374 21.226389415080778 74-75 20.52461300361193 29.554325038080243 28.231598992526163 21.689462965781665 76-77 19.396426734275998 30.035341577849795 30.44908579637429 20.11914589149991 78-79 19.915440192073312 28.974038772410683 29.34661966307177 21.763901372444234 80-81 19.716789638173793 30.24093601296784 28.578737502402586 21.46353684645579 82-83 20.76848211200647 29.605487554898325 28.612734744848474 21.01329558824673 84-85 20.301575538872957 29.309400459740488 28.4189726833268 21.97005131805976 86-87 18.77158852670839 30.11616835523341 30.371536530925823 20.740706587132376 88-89 19.83505782308768 28.68117363813824 29.986456654071393 21.497311884702686 90-91 19.894497446318244 29.54865883100593 29.681592493053344 20.875251229622485 92-93 20.512447323717076 29.583100481849804 29.418891578794167 20.485560615638953 94-95 19.982990268567104 28.19121337935923 29.66881575161126 22.156980600462404 96-97 20.53644537720829 28.487967087114047 29.749698080044617 21.225889455633045 98-99 19.79003890246497 28.421742050492654 30.01811522751248 21.770103819529893 100-101 19.624215611334307 29.065377573150354 30.054196146051837 21.256210669463506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 36.0 1 28.5 2 57.0 3 108.0 4 88.5 5 71.5 6 118.0 7 217.5 8 380.5 9 447.5 10 362.0 11 289.5 12 290.0 13 306.5 14 311.5 15 317.5 16 355.5 17 396.0 18 438.0 19 495.0 20 525.5 21 578.0 22 676.0 23 810.0 24 897.5 25 974.5 26 1277.5 27 1725.0 28 2031.5 29 2541.5 30 4089.5 31 6488.5 32 12419.0 33 21659.0 34 31641.0 35 46114.0 36 70648.5 37 88610.0 38 82309.5 39 70936.0 40 57715.5 41 43271.5 42 35659.0 43 32333.5 44 31446.5 45 30748.5 46 27786.0 47 22871.0 48 21354.5 49 19787.5 50 17924.5 51 15291.5 52 12110.0 53 10533.5 54 9852.5 55 9268.0 56 8882.5 57 7908.5 58 6566.5 59 6356.5 60 5812.5 61 4423.0 62 3289.5 63 2301.0 64 1423.5 65 940.0 66 589.5 67 403.0 68 331.0 69 187.5 70 134.5 71 125.0 72 88.0 73 64.5 74 51.5 75 58.5 76 52.0 77 33.5 78 22.0 79 10.0 80 6.5 81 4.0 82 2.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021220501003807467 2 0.10654691341702284 3 0.0 4 0.0 5 0.0 6 0.0053329007758259606 7 0.029997566864021028 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001222123094460116 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.555104974818709E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 5.555104974818709E-5 62-63 4.444083979854967E-4 64-65 3.3330629848912253E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.6665314924456127E-4 100-101 9.999188954673676E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 900073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.730351682852998 #Duplication Level Percentage of deduplicated Percentage of total 1 62.67156001886109 9.858456796097142 2 12.80351862433203 4.028077014774021 3 5.866275611020992 2.7683573528971066 4 3.570049945897501 2.24632564697272 5 2.2854100416169856 1.7975151847079447 6 1.6406137494239506 1.5484458752497685 7 1.275155289585725 1.4041048808803596 8 0.9901969709073659 1.2460917270134901 9 0.8339568268619507 1.1806590757369169 >10 6.227057383010139 19.873079745259624 >50 0.9144967096604584 10.194202139392806 >100 0.8048724984077852 25.840657977630205 >500 0.08077573460723292 8.563483758502812 >1k 0.035339383890664405 8.820105782822637 >5k 7.212119161360082E-4 0.6304370420624528 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 5557 0.6173943669013513 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3466 0.3850798768544329 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3106 0.34508312103573824 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2572 0.28575459990467444 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2550 0.28331035371575414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2527 0.28075500542733756 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 2420 0.2688670807812255 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2320 0.2577568708315881 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2187 0.24298029159857035 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2163 0.24031384121065735 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1996 0.22175979059476286 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1905 0.21164949954059284 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1807 0.20076149378994818 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1785 0.1983172476010279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1685 0.1872070376513905 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1681 0.186762629253405 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1643 0.18254074947254278 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1618 0.17976319698513343 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1609 0.17876327808966608 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1563 0.17365258151283283 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1512 0.16798637443851777 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1482 0.16465331145362655 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1471 0.16343118835916642 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1471 0.16343118835916642 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 1414 0.1570983686878731 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1391 0.1545430203994565 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1383 0.1536542036034855 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1369 0.15209877421053625 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1347 0.14965452802161602 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1333 0.1480990986286668 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1320 0.14665477133521393 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1276 0.14176627895737345 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1272 0.14132187055938797 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 1240 0.13776660337550398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.13532235718658375 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1217 0.13521125508708737 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1211 0.13454464249010914 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 1193 0.1325448046991744 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1159 0.1287673333162977 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 1153 0.12810072071931944 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1136 0.12621198502788109 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1121 0.12454545353543546 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1103 0.12254561574450074 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 1078 0.11976806325709136 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 1078 0.11976806325709136 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1068 0.11865704226212764 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1044 0.11599059187421465 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1033 0.11476846877975452 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1031 0.11454626458076178 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1018 0.11310193728730893 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 998 0.11087989529738143 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 982 0.10910226170543945 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 977 0.10854675120795756 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 974 0.10821344490946846 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 969 0.1076579344119866 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 966 0.10732462811349747 No Hit GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA 961 0.10676911761601558 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 939 0.10432487142709537 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 933 0.10365825883011712 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 917 0.10188062523817512 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 914 0.101547318939686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.3330629848912253E-4 0.0 12-13 0.0 0.0 0.0 6.11061547230058E-4 0.0 14-15 0.0 0.0 0.0 7.221636467264322E-4 0.0 16-17 0.0 0.0 0.0 9.443678457191807E-4 0.0 18-19 0.0 0.0 0.0 0.0011665720447119289 0.0 20-21 0.0 0.0 0.0 0.001833184641690174 0.0 22-23 0.0 0.0 0.0 0.0033886140346394127 0.0 24-25 0.0 0.0 0.0 0.00649947282053789 0.0 26-27 0.0 0.0 0.0 0.00838820851197625 0.0 28-29 1.1110209949637419E-4 0.0 0.0 0.010776903651148296 0.0 30-31 1.1110209949637419E-4 0.0 0.0 0.014332170835032269 0.0 32-33 1.1110209949637419E-4 0.0 0.0 0.021609358352044777 0.0 34-35 1.1110209949637419E-4 0.0 0.0 0.03260846620218582 0.0 36-37 1.1110209949637419E-4 0.0 0.0 0.05044035317135388 0.0 38-39 1.1110209949637419E-4 0.0 0.0 0.08660408655742367 0.0 40-41 1.1110209949637419E-4 0.0 0.0 0.1307671711072324 0.0 42-43 1.1110209949637419E-4 0.0 0.0 0.177263399746465 0.0 44-45 1.1110209949637419E-4 0.0 0.0 0.22492620043040953 0.0 46-47 1.1110209949637419E-4 0.0 0.0 0.2857545999046744 0.0 48-49 1.1110209949637419E-4 0.0 0.0 0.3521936554035061 0.0 50-51 1.1110209949637419E-4 0.0 0.0 0.4291874103544935 0.0 52-53 1.1110209949637419E-4 0.0 0.0 0.5119584744792922 0.0 54-55 1.1110209949637419E-4 0.0 0.0 0.5980070505392341 0.0 56-57 1.1110209949637419E-4 0.0 0.0 0.6937770603051086 0.0 58-59 1.1110209949637419E-4 0.0 0.0 0.8103787137265532 0.0 60-61 1.1110209949637419E-4 0.0 0.0 0.9390349449433546 0.0 62-63 1.1110209949637419E-4 0.0 0.0 1.0685244419063786 0.0 64-65 1.1110209949637419E-4 0.0 0.0 1.2212898287138931 0.0 66-67 1.1110209949637419E-4 0.0 0.0 1.3837766492273404 0.0 68-69 1.1110209949637419E-4 0.0 0.0 1.568594991739559 0.0 70-71 1.1110209949637419E-4 0.0 0.0 1.7879660871951497 0.0 72-73 1.1110209949637419E-4 0.0 0.0 2.0212249450877873 0.0 74-75 1.1110209949637419E-4 0.0 0.0 2.2672049933727596 0.0 76-77 1.1110209949637419E-4 0.0 0.0 2.531183581776145 0.0 78-79 1.1110209949637419E-4 0.0 0.0 2.838880846331353 0.0 80-81 1.1110209949637419E-4 0.0 0.0 3.1753535546561222 0.0 82-83 1.1110209949637419E-4 0.0 0.0 3.546990077471494 0.0 84-85 1.1110209949637419E-4 0.0 0.0 3.932125505375675 0.0 86-87 1.1110209949637419E-4 0.0 0.0 4.362368385675384 0.0 88-89 1.1110209949637419E-4 0.0 0.0 4.8237754048838255 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 455 0.0 66.92884 1 CATATAG 580 0.0 51.606598 3 ACATATA 695 0.0 44.49393 2 GGGATCC 120 3.787136E-9 35.629166 7 GTACATG 5445 0.0 35.39174 1 TCGCCCG 35 2.0543924E-4 33.926884 82-83 GTATCAA 3720 0.0 33.64012 1 ACATGGG 5390 0.0 33.319286 3 TAACTTT 460 0.0 33.051018 6 ATATAGG 975 0.0 32.161182 4 ATCTATA 475 0.0 32.055393 1 CATGGGA 2890 0.0 31.893085 4 GGTATCA 2165 0.0 31.868004 1 TAAGATC 120 1.4847137E-7 31.71619 2 GTACATT 60 0.003946092 31.67389 6 ATACTCG 60 0.003946092 31.67389 6 CGGGCTC 30 0.003952503 31.66509 82-83 ACCCTTG 505 0.0 31.043234 7 CATGGGG 1940 0.0 30.857552 4 GTAATAC 125 2.1487358E-7 30.406935 3 >>END_MODULE