FastQCFastQC Report
Fri 10 Feb 2017
SRR2568664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131795
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28100.037.8700071
TCAACGC28750.035.1920434
ATCAACG28850.035.070063
CAACGCA30050.033.8276675
AACGCAG33450.030.5312826
TATCAAC35050.030.2245852
GGTATCA16150.030.004151
ACGCAGA38900.025.887437
CGCAGAG39350.025.7121038
GTACATG55650.025.1831761
TACATGG57100.023.6279322
ACATGGG55750.022.834563
GCAGAGT53300.018.6260959
CATGGGG36850.018.304324
GTATATA17550.017.5950261
AGAGTAC44900.017.08514810-11
GTGTAGG11150.017.0427151
GTATAAC7150.016.610691
GTACCTG16100.016.5240231
GTAGGAC6100.016.3528023
Produced by FastQC (version 0.11.5)