##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568664_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2131795 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.892392561198427 33.0 31.0 34.0 30.0 34.0 2 32.03262415007072 34.0 31.0 34.0 30.0 34.0 3 32.082834887969994 34.0 31.0 34.0 30.0 34.0 4 35.40933157268874 37.0 35.0 37.0 33.0 37.0 5 35.450120672954014 37.0 35.0 37.0 33.0 37.0 6 35.56725060336477 37.0 35.0 37.0 33.0 37.0 7 35.554400399663194 37.0 35.0 37.0 33.0 37.0 8 35.56932584981201 37.0 35.0 37.0 33.0 37.0 9 37.34643903377201 39.0 38.0 39.0 34.0 39.0 10-11 37.31714447214671 39.0 38.0 39.0 34.0 39.0 12-13 37.28878433432858 39.0 38.0 39.0 34.0 39.0 14-15 38.68602163904127 41.0 39.0 41.0 34.0 41.0 16-17 38.65770043554845 41.0 39.0 41.0 34.0 41.0 18-19 38.64016615106049 41.0 39.0 41.0 34.0 41.0 20-21 38.63365145335269 41.0 39.0 41.0 34.0 41.0 22-23 38.5697459183458 41.0 39.0 41.0 34.0 41.0 24-25 38.51511871451054 41.0 39.0 41.0 34.0 41.0 26-27 38.42500263862144 40.0 38.0 41.0 34.0 41.0 28-29 38.308572118801294 40.0 38.0 41.0 33.5 41.0 30-31 38.231649619217606 40.0 38.0 41.0 33.0 41.0 32-33 38.12949556594326 40.0 38.0 41.0 33.0 41.0 34-35 37.998134670547586 40.0 38.0 41.0 33.0 41.0 36-37 37.90534596431645 40.0 38.0 41.0 33.0 41.0 38-39 37.809066772367885 40.0 38.0 41.0 32.0 41.0 40-41 37.68489441996064 40.0 38.0 41.0 32.0 41.0 42-43 37.53429551152902 40.0 37.5 41.0 31.0 41.0 44-45 37.371470521321235 40.0 37.0 41.0 31.0 41.0 46-47 37.44352998294865 40.0 37.5 41.0 31.5 41.0 48-49 37.639192323839765 40.0 38.0 41.0 32.0 41.0 50-51 36.54444517413729 39.0 36.0 40.0 30.0 40.5 52-53 36.69332745409385 39.0 36.5 40.0 30.5 40.5 54-55 37.30819262640169 40.0 37.0 41.0 31.0 41.0 56-57 37.29790645911075 40.0 37.0 41.0 31.0 41.0 58-59 37.15685959484847 40.0 36.0 41.0 31.0 41.0 60-61 36.95856754519079 39.5 36.0 41.0 30.5 41.0 62-63 36.70911602663483 39.0 35.0 41.0 30.0 41.0 64-65 36.54879104229065 39.0 35.0 41.0 30.0 41.0 66-67 36.436896371367794 39.0 35.0 41.0 30.0 41.0 68-69 36.15860436861894 38.5 35.0 41.0 30.0 41.0 70-71 35.81519892860243 37.0 35.0 40.0 29.0 41.0 72-73 35.46832200094286 37.0 35.0 39.5 29.0 41.0 74-75 35.11272050079862 36.5 34.5 39.0 29.0 41.0 76-77 34.739874612709 36.0 34.0 39.0 29.0 40.5 78-79 34.36474661025099 35.5 34.0 37.5 28.5 39.5 80-81 33.9812472587655 35.0 34.0 37.0 28.0 39.0 82-83 33.645062728827114 35.0 34.0 37.0 27.5 39.0 84-85 33.32369998053284 35.0 34.0 36.0 27.0 37.0 86-87 33.06687861637728 35.0 34.0 36.0 26.5 37.0 88-89 32.84126991572829 35.0 33.5 35.5 26.0 37.0 90-91 32.65828538860444 35.0 33.0 35.0 26.0 36.0 92-93 32.49717890322475 35.0 33.0 35.0 26.0 36.0 94-95 32.3502597576221 35.0 33.0 35.0 25.0 36.0 96-97 32.2413693624387 35.0 33.0 35.0 25.0 36.0 98-99 32.110421968341235 35.0 33.0 35.0 25.0 35.0 100-101 30.80064781088238 34.0 31.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29827519887097154 1101 2 0.3110931150756997 1101 3 0.35245585767130194 1101 4 0.27871867069116263 1101 5 0.1881001308968422 1101 6 0.23788042768577355 1101 7 0.22490099063956137 1101 8 0.25849809451017336 1101 9 0.45876745075037206 1101 10-11 0.29536815901593627 1101 12-13 0.4248963924987308 1101 14-15 0.4089409353676743 1101 16-17 0.4006212203484836 1101 18-19 0.46357479414587743 1101 20-21 0.2641565830326513 1101 22-23 0.2382893853846042 1101 24-25 0.28122902802062555 1101 26-27 0.3245511866029389 1101 28-29 0.4404395718287901 1101 30-31 0.4364393726329254 1101 32-33 0.4192975698682275 1101 34-35 0.25719770144318943 1101 36-37 0.38758164189342637 1101 38-39 0.43729580035195426 1101 40-41 0.40006486145614417 1101 42-43 0.48929627998683856 1101 44-45 0.48014657664473503 1101 46-47 0.41399918881417364 1101 48-49 0.41325133191927677 1101 50-51 0.09804579557336268 1101 52-53 0.3078335410413544 1101 54-55 0.41823252390689447 1101 56-57 0.3523926272988689 1101 58-59 0.29304358270820074 1101 60-61 0.43924093859646973 1101 62-63 0.3730834593473702 1101 64-65 0.28384474442842844 1101 66-67 0.2889480647084923 1101 68-69 0.3069031722970976 1101 70-71 0.3428948988924674 1101 72-73 0.2275068376657785 1101 74-75 0.30403622645567907 1101 76-77 0.14521842260435847 1101 78-79 0.203933038199672 1101 80-81 0.23629087294104778 1101 82-83 0.2195566286685704 1101 84-85 0.1635343575591719 1101 86-87 0.17812958302179283 1101 88-89 0.24488656366284545 1101 90-91 0.26174327193314895 1101 92-93 0.2597634347960778 1101 94-95 0.1944997852958963 1101 96-97 0.07215780545678285 1101 98-99 0.13897264914414365 1101 100-101 -0.12289522239852602 1102 1 0.11653674531622826 1102 2 0.1261118242773449 1102 3 0.21460045112171855 1102 4 0.1538632914542788 1102 5 0.08840931069637037 1102 6 0.10198061068089004 1102 7 0.0809635913551574 1102 8 0.22699671521866094 1102 9 0.14293509612223687 1102 10-11 0.2027936636181238 1102 12-13 0.22286159611493872 1102 14-15 0.16573565309089133 1102 16-17 0.14639007294182704 1102 18-19 0.1331062447235425 1102 20-21 0.10083481207698242 1102 22-23 0.05221353800799022 1102 24-25 0.11262989656457734 1102 26-27 0.10359387635794093 1102 28-29 0.13239966796950853 1102 30-31 0.20344002813779838 1102 32-33 0.08960702279478028 1102 34-35 0.04245293865401578 1102 36-37 -0.025081210099038742 1102 38-39 0.024321446980202666 1102 40-41 0.064549334184413 1102 42-43 -0.13053683939698857 1102 44-45 -0.032498248963655385 1102 46-47 0.17927832464023652 1102 48-49 0.16157296631143225 1102 50-51 0.08616572565284031 1102 52-53 -0.03363683833209308 1102 54-55 0.050141545292738954 1102 56-57 0.08373381758646303 1102 58-59 0.011799489080253522 1102 60-61 0.011010665196316438 1102 62-63 0.06617607672371406 1102 64-65 0.004637359073498715 1102 66-67 -0.05705103397230715 1102 68-69 -0.0849159902954213 1102 70-71 -0.05106749151531176 1102 72-73 -0.04837522608207223 1102 74-75 -0.052946539228642564 1102 76-77 -0.14348203898032352 1102 78-79 -0.09059991457666428 1102 80-81 -0.11566635536583902 1102 82-83 -0.10294644401052722 1102 84-85 0.10703516328391771 1102 86-87 0.0019745288060732946 1102 88-89 -0.05795914687712411 1102 90-91 0.03515490168214086 1102 92-93 0.02894487308302729 1102 94-95 -0.0885108939710264 1102 96-97 -0.11370186187449605 1102 98-99 -0.05365210162744205 1102 100-101 -0.1731709441367073 1103 1 0.14781496875592026 1103 2 0.17334950642926827 1103 3 0.3092474113428736 1103 4 0.20275410946317862 1103 5 0.23843424532247326 1103 6 0.24393871998982064 1103 7 0.16486413010403567 1103 8 0.28705658428137326 1103 9 0.2712239421539664 1103 10-11 0.21752897626666368 1103 12-13 0.37236486357637943 1103 14-15 0.3255288345804388 1103 16-17 0.3788707398450839 1103 18-19 0.3976348504898439 1103 20-21 0.28951399858253524 1103 22-23 0.37815102673159373 1103 24-25 0.3996556097325268 1103 26-27 0.39814930144983407 1103 28-29 0.36231353512623343 1103 30-31 0.38093043395305415 1103 32-33 0.3743666833723438 1103 34-35 0.38727194909332496 1103 36-37 0.2421398420461358 1103 38-39 0.2649067110704735 1103 40-41 0.26125835293873223 1103 42-43 0.3592141336505037 1103 44-45 0.43511866795355303 1103 46-47 0.3388986122100732 1103 48-49 0.38551641182765195 1103 50-51 0.3885540760293722 1103 52-53 0.3200650515334402 1103 54-55 0.46560212976245197 1103 56-57 0.45323291586420567 1103 58-59 0.4209356866549143 1103 60-61 0.4591347012672884 1103 62-63 0.4258572146460722 1103 64-65 0.4895544413821753 1103 66-67 0.3519557527630468 1103 68-69 0.42564741283842267 1103 70-71 0.4712389393337375 1103 72-73 0.41837358682983705 1103 74-75 0.4816310765122864 1103 76-77 0.37181777491208834 1103 78-79 0.39057385860987637 1103 80-81 0.35477722137841994 1103 82-83 0.35827638328272826 1103 84-85 0.36178124416036894 1103 86-87 0.23057936054213002 1103 88-89 0.23422781323500175 1103 90-91 0.2657463260918078 1103 92-93 0.1454294256862667 1103 94-95 0.12205066599940295 1103 96-97 0.22995881122282213 1103 98-99 0.2079126454580873 1103 100-101 0.22182806029545077 1104 1 0.118877608509532 1104 2 0.1261343325772799 1104 3 0.13801070865418552 1104 4 0.14232678736839688 1104 5 0.1320316464326723 1104 6 -0.037921728306614 1104 7 0.12033535969473519 1104 8 0.176747935981318 1104 9 0.059471318854370736 1104 10-11 0.1127365800685709 1104 12-13 0.05852987379233099 1104 14-15 0.18583694044060195 1104 16-17 0.21480955262496337 1104 18-19 0.18215120505266924 1104 20-21 0.12429608842263207 1104 22-23 0.13141874492804106 1104 24-25 0.1705758891493474 1104 26-27 0.20807052712291352 1104 28-29 0.30600393454280095 1104 30-31 0.24327196616494007 1104 32-33 0.27508704355243907 1104 34-35 0.22424272363722508 1104 36-37 0.2104066260405375 1104 38-39 0.007625540364635697 1104 40-41 0.11142186843141388 1104 42-43 0.008605094191018736 1104 44-45 0.1383678829224877 1104 46-47 0.1445794044134061 1104 48-49 0.2844117631177525 1104 50-51 0.18144148358857848 1104 52-53 0.08334717452265039 1104 54-55 0.08840690564436926 1104 56-57 0.28300454866855773 1104 58-59 0.23823698790795333 1104 60-61 0.26354341164656603 1104 62-63 0.18724959758453252 1104 64-65 0.12841037537326372 1104 66-67 0.108741977200566 1104 68-69 0.23233437077521302 1104 70-71 0.2349526033946887 1104 72-73 0.22452015408936887 1104 74-75 0.18649025308853595 1104 76-77 0.12357606369503316 1104 78-79 0.19086074904304695 1104 80-81 0.19794591415854512 1104 82-83 0.1814558043185457 1104 84-85 0.17839485216919115 1104 86-87 0.17088481447391501 1104 88-89 0.145615796383197 1104 90-91 0.13082380459008647 1104 92-93 0.12339057493558414 1104 94-95 0.16121506747725078 1104 96-97 -0.02197028585583638 1104 98-99 0.05254906005092863 1104 100-101 -0.10970879246827892 1105 1 0.2505522600038965 1105 2 0.31183462111224003 1105 3 0.45666520324499515 1105 4 0.3437312160759376 1105 5 0.30220645672262236 1105 6 0.2789023599702176 1105 7 0.32805593352116347 1105 8 0.3828467676512375 1105 9 0.463799478379741 1105 10-11 0.4740153237589766 1105 12-13 0.5199933609434026 1105 14-15 0.5839335557295726 1105 16-17 0.4952640458643245 1105 18-19 0.5466870827192523 1105 20-21 0.6300892216960534 1105 22-23 0.48064983642808556 1105 24-25 0.5741953473523225 1105 26-27 0.61894295683053 1105 28-29 0.6362159097571833 1105 30-31 0.6240195892990954 1105 32-33 0.6075716201088 1105 34-35 0.5524998238271053 1105 36-37 0.5535223981587407 1105 38-39 0.4587802883526635 1105 40-41 0.5617772326814574 1105 42-43 0.48470466915943433 1105 44-45 0.6200354392949166 1105 46-47 0.6219805626075967 1105 48-49 0.6369931036227925 1105 50-51 0.5430088973828404 1105 52-53 0.4685503990866309 1105 54-55 0.5739713732737215 1105 56-57 0.5255412236917039 1105 58-59 0.522889495864618 1105 60-61 0.5510555916058877 1105 62-63 0.5790727736314025 1105 64-65 0.6736230600998283 1105 66-67 0.5558434509587471 1105 68-69 0.5044095925822418 1105 70-71 0.5366043477810933 1105 72-73 0.6232110027335196 1105 74-75 0.5231940621554259 1105 76-77 0.5235574136602281 1105 78-79 0.44965092939428786 1105 80-81 0.4834175370494407 1105 82-83 0.4531562685422301 1105 84-85 0.5178737323562288 1105 86-87 0.4419845583737967 1105 88-89 0.5305638668478991 1105 90-91 0.27637502799182556 1105 92-93 0.3401028981487997 1105 94-95 0.3468087264654258 1105 96-97 0.2623747668169969 1105 98-99 0.30852632863170726 1105 100-101 0.1870612377366676 1106 1 0.26692839752417186 1106 2 0.16759193789030746 1106 3 0.2625821835871065 1106 4 0.23450687858463226 1106 5 0.2073285048620619 1106 6 0.299121915243056 1106 7 0.1146136561417066 1106 8 0.16992514698985417 1106 9 0.2750786833773944 1106 10-11 0.1341795815115674 1106 12-13 0.2513893926759465 1106 14-15 0.25836057031362714 1106 16-17 0.244265558466509 1106 18-19 0.1809423291449903 1106 20-21 0.26991846247874207 1106 22-23 0.26125804440589917 1106 24-25 0.17778000141370143 1106 26-27 0.2351212987519986 1106 28-29 0.28331937234017346 1106 30-31 0.25115330392579693 1106 32-33 0.15755938602477926 1106 34-35 0.3384323537304752 1106 36-37 0.3555944770115289 1106 38-39 0.3308881144705893 1106 40-41 0.39575585662082347 1106 42-43 0.38000411016835045 1106 44-45 0.40764701967149364 1106 46-47 0.29151380869721066 1106 48-49 0.21614492954972064 1106 50-51 0.22731328247242288 1106 52-53 0.35086700854850505 1106 54-55 0.4742828770625138 1106 56-57 0.4527651495134961 1106 58-59 0.3244445598986516 1106 60-61 0.31111215704663664 1106 62-63 0.3396007627067803 1106 64-65 0.2855221703163764 1106 66-67 0.30794745712893246 1106 68-69 0.2863473635833955 1106 70-71 0.26509522293489596 1106 72-73 0.3046570363949215 1106 74-75 0.3152215129041309 1106 76-77 0.44652395917304943 1106 78-79 0.24601817528481007 1106 80-81 0.2687287859052745 1106 82-83 0.23558191916754367 1106 84-85 0.25454255988316277 1106 86-87 0.29467080982617944 1106 88-89 0.2999882066592292 1106 90-91 0.13526141071444187 1106 92-93 0.13536354618566548 1106 94-95 0.19120898253141405 1106 96-97 0.21397041779571424 1106 98-99 0.21691016971324473 1106 100-101 0.008244115846071765 1107 1 0.10531223590801275 1107 2 0.08000292347202276 1107 3 0.23091827387441555 1107 4 0.2046907146221315 1107 5 0.29264156578487643 1107 6 0.12673603554009816 1107 7 0.23478165435848553 1107 8 0.2378752637204613 1107 9 0.30251917423252195 1107 10-11 0.27252881132420725 1107 12-13 0.2775041992785532 1107 14-15 0.29203060398366176 1107 16-17 0.2537917143341275 1107 18-19 0.24218483550944825 1107 20-21 0.23948345033640805 1107 22-23 0.24254008666354565 1107 24-25 0.26834593954061603 1107 26-27 0.2729032999486307 1107 28-29 0.3502781588111503 1107 30-31 0.32706339203100754 1107 32-33 0.08767060272136007 1107 34-35 0.09722527715754126 1107 36-37 0.14254645908545882 1107 38-39 0.06727345451446354 1107 40-41 0.053565075405650475 1107 42-43 0.04967996277103026 1107 44-45 0.22198963279459605 1107 46-47 0.1817577111362354 1107 48-49 0.26297795199249663 1107 50-51 0.12617706548254404 1107 52-53 0.14654152373521612 1107 54-55 0.25494079186676544 1107 56-57 0.21334422716574153 1107 58-59 0.22777519493660492 1107 60-61 0.2638215975609626 1107 62-63 0.38610822994596106 1107 64-65 0.4203958783608215 1107 66-67 0.33786334070335045 1107 68-69 0.18544284804472966 1107 70-71 0.3261500217945681 1107 72-73 0.4491742174974718 1107 74-75 0.452511955072616 1107 76-77 0.42425422348868125 1107 78-79 0.3395111773143995 1107 80-81 0.310965907410683 1107 82-83 0.32615079022421867 1107 84-85 0.3233520250340902 1107 86-87 0.35158724479138925 1107 88-89 0.20994433002998392 1107 90-91 0.3152054504145738 1107 92-93 0.23105331260713768 1107 94-95 0.2755072497565081 1107 96-97 0.24043717596978098 1107 98-99 0.2745158431725727 1107 100-101 0.10327104014129063 1108 1 0.07939125044945072 1108 2 0.0984690248367599 1108 3 0.10102013185164083 1108 4 0.09272466165791116 1108 5 -0.011550240028249448 1108 6 0.0841536251302557 1108 7 0.07900550492661296 1108 8 0.008437950820578521 1108 9 0.044698273806233146 1108 10-11 -0.12293260623646063 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-0.15918920323688468 2316 96-97 -0.08033704899778371 2316 98-99 -0.15257285217899508 2316 100-101 0.06991865587256996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 89.0 7 1231.0 8 2030.0 9 1273.0 10 1104.0 11 1579.0 12 2473.0 13 3447.0 14 4119.0 15 4704.0 16 5327.0 17 5938.0 18 6550.0 19 7611.0 20 8692.0 21 9555.0 22 10871.0 23 11639.0 24 12721.0 25 13780.0 26 15352.0 27 17596.0 28 20596.0 29 24147.0 30 28767.0 31 35347.0 32 45116.0 33 58919.0 34 82051.0 35 123822.0 36 216869.0 37 428750.0 38 670736.0 39 248942.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48372302926074 19.372536546600728 13.202655955775352 23.941084468363183 2 14.61950966185048 20.293052596814366 41.839370885486275 23.24806685584888 3 18.773105766889813 25.663801915119482 29.262168157679074 26.300924160311627 4 11.832660501570045 16.844795866001558 40.09032801603914 31.232215616389265 5 13.14869413268597 38.151966130715735 35.78210134984578 12.917238386752514 6 30.465925440483222 38.28496880179275 18.657705401293715 12.591400356430313 7 27.64672382314143 31.967704527670975 22.728940203368968 17.656631445818626 8 24.770184715383692 37.147841734718384 20.69393051218926 17.38804303770866 9 25.48567407494283 16.773775875212575 20.461291268398522 37.27925878144608 10-11 24.15477169488512 26.897022625270743 29.504565257878546 19.44364042196559 12-13 24.801128667377363 23.847765944596315 29.602515342675567 21.748590045350756 14-15 21.824682954974563 25.91506218937562 26.52199203019052 25.738262825459294 16-17 22.09513578932308 29.208741928750186 26.909974927232682 21.786147354694048 18-19 22.005247698942416 28.547327447360587 28.185237627601012 21.262187226095985 20-21 22.684419976531256 27.680710620251748 28.15963155525815 21.475237847958844 22-23 22.335418749228932 27.54963084995748 27.887539526660472 22.227410874153115 24-25 22.168789599019213 27.64846238590123 27.98496829263888 22.19777972244068 26-27 22.214299519446378 27.926362334803827 27.80683376897774 22.052504376772053 28-29 22.28493061877019 27.83559318893306 27.803154845826395 22.076321346470355 30-31 22.482260686397083 27.657120472516674 27.75707050922679 22.103548331859454 32-33 22.23541409448612 27.731538467311882 27.768455903599985 22.264591534602005 34-35 22.428892083901125 27.94241472561855 27.709676587101477 21.919016603378843 36-37 22.49781170225641 27.739031069606163 27.701105266268694 22.062051961868736 38-39 22.32753531478102 27.9298186525277 27.652821582223215 22.08982445046807 40-41 22.497167879650718 27.711599848953583 27.7040006191965 22.0872316521992 42-43 22.347602106776097 27.658636787522962 27.741243500760866 22.252517604940078 44-45 22.45935321656081 27.77364455994521 27.601839777087694 22.16516244640629 46-47 22.389060918980004 27.790042186333842 27.583121338366485 22.23777555631967 48-49 22.30642374284294 27.767143455920312 27.665183751465072 22.261249049771674 50-51 22.347301158852673 27.888670207937672 27.535973876500226 22.22805475670943 52-53 22.388245586465867 27.78259635659151 27.614686215137947 22.214471841804677 54-55 22.298813910343164 27.75339561261754 27.671117532408136 22.27667294463117 56-57 22.356206858539398 27.906224566621084 27.573547175033248 22.164021399806266 58-59 22.31359019042638 27.742630037128336 27.69759756449377 22.246182207951513 60-61 22.420448495282145 27.775818031283496 27.736039347122965 22.067694126311395 62-63 22.259278031700894 27.77129516113257 27.675928377810077 22.293498429356458 64-65 22.305824195206895 27.68256550554292 27.688476203343015 22.32313409590717 66-67 22.285934496995147 27.71444078698928 27.597962020322562 22.401662695693005 68-69 22.247049482376646 27.71160867275672 27.700302704401704 22.34103914046493 70-71 22.208316279920677 27.509396479237445 27.767607624136136 22.514679616705738 72-73 22.2057233458189 27.531118142222873 27.765591907289394 22.497566604668837 74-75 22.18761653911375 27.5998864806419 27.712467662228306 22.500029318016036 76-77 22.09640232761593 27.542962620702273 27.738408242818846 22.62222680886295 78-79 22.176897872450212 27.456533109421873 27.75119089781147 22.615378120316446 80-81 22.2039771153188 27.570274873743212 27.605485323622975 22.62026268731502 82-83 22.2747903549557 27.532307351633424 27.692873558041576 22.5000287353693 84-85 22.273656554045733 27.601625682499282 27.62287919396175 22.501838569493234 86-87 22.13417826754394 27.545030420634237 27.740859734790625 22.5799315770312 88-89 22.19354596561273 27.41668553091019 27.73266649698622 22.657102006490863 90-91 22.265546470808324 27.46655187904137 27.69254588084059 22.575355769309716 92-93 22.263062993749486 27.346276912784834 27.82599047797542 22.56466961549026 94-95 22.12487580805409 27.29489707464185 27.883487720393052 22.69673939691101 96-97 22.11150542242564 27.256552640091353 27.875651897936937 22.75629003954607 98-99 22.146906173578003 27.199540221796063 27.78990951048869 22.863644094137243 100-101 22.31179062575066 27.029715837436946 27.856591925894786 22.801901610917607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17.0 1 14.5 2 18.0 3 25.5 4 23.5 5 23.5 6 40.5 7 70.0 8 124.5 9 153.0 10 141.0 11 141.0 12 151.5 13 192.5 14 215.0 15 228.5 16 288.0 17 326.0 18 361.0 19 408.5 20 486.5 21 558.0 22 623.0 23 781.0 24 965.0 25 1264.0 26 1827.5 27 2806.0 28 4620.5 29 8093.5 30 13655.0 31 22296.0 32 33333.5 33 44927.0 34 57530.5 35 70168.0 36 83611.5 37 94025.5 38 97839.5 39 98472.5 40 96069.5 41 91368.5 42 86649.0 43 82431.0 44 78727.5 45 76111.5 46 74553.5 47 73401.0 48 73146.5 49 73258.5 50 71371.5 51 67820.5 52 65038.0 53 63173.5 54 60811.0 55 56824.5 56 51052.5 57 45211.0 58 40320.0 59 35062.0 60 29299.0 61 24096.0 62 19417.5 63 14826.0 64 10607.0 65 7710.5 66 5777.0 67 4224.0 68 3108.0 69 2341.5 70 1804.0 71 1351.0 72 997.5 73 744.0 74 571.5 75 441.0 76 330.5 77 222.5 78 142.0 79 110.5 80 85.0 81 64.0 82 52.5 83 47.0 84 30.5 85 25.5 86 24.5 87 17.0 88 21.0 89 22.0 90 13.0 91 7.0 92 6.0 93 5.0 94 3.5 95 4.0 96 3.0 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00623887381291353 2 0.007693047408404654 3 0.0010319941645420877 4 4.2217943094903585E-4 5 0.003283617796270279 6 0.004784700217422407 7 0.004831609043083411 8 0.003893432529863331 9 0.007974500362370678 10-11 0.007130141500472606 12-13 0.003635433988727809 14-15 0.0 16-17 0.0 18-19 1.6418088981351396E-4 20-21 0.0011023574030335937 22-23 2.814529539660239E-4 24-25 0.0016183544853046374 26-27 0.004081067832507347 28-29 0.0027441663011687333 30-31 0.010789029902030917 32-33 5.159970822710438E-4 34-35 0.0 36-37 4.690882566100399E-5 38-39 1.6418088981351396E-4 40-41 0.0 42-43 3.283617796270279E-4 44-45 7.036323849150598E-4 46-47 0.0029083471909822473 48-49 0.003307072209100781 50-51 0.003494707511744797 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0011961750543556017 64-65 0.003142891319287267 66-67 0.003565070750236303 68-69 0.008326316554828208 70-71 5.863603207625498E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015268822752656798 82-83 0.012923381469606598 84-85 0.017942625815334026 86-87 0.016277362504368384 88-89 0.015831728660588847 90-91 0.015128096275673786 92-93 0.009874307801641339 94-95 0.024275317279569567 96-97 0.010812484314861419 98-99 0.015597184532283826 100-101 0.015902091899080352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2131795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.804585724802834 #Duplication Level Percentage of deduplicated Percentage of total 1 68.9739475583608 37.11114674181879 2 15.930718104972744 17.142913758733496 3 6.24496943589974 10.080239800879234 4 3.0883773748477403 6.64675460862147 5 1.721336435309457 4.630789689741711 6 1.089523149429209 3.517280501557263 7 0.6882099841436449 2.5920197161965364 8 0.49337438969546765 2.1236643715833656 9 0.3337718646082476 1.616261121165757 >10 1.3824402456158247 11.957995665138586 >50 0.04025892136883529 1.4492648886980317 >100 0.012809686554689954 1.0432785610444253 >500 2.62849193631259E-4 0.08839057482142146 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.690882566100399E-5 0.0 2 0.0 0.0 0.0 4.690882566100399E-5 0.0 3 0.0 0.0 0.0 4.690882566100399E-5 0.0 4 0.0 0.0 0.0 9.381765132200798E-5 0.0 5 0.0 0.0 0.0 9.381765132200798E-5 0.0 6 0.0 0.0 0.0 9.381765132200798E-5 0.0 7 0.0 0.0 0.0 9.381765132200798E-5 0.0 8 0.0 0.0 0.0 9.381765132200798E-5 0.0 9 0.0 0.0 0.0 1.4072647698301196E-4 0.0 10-11 0.0 0.0 0.0 2.3454412830501995E-4 2.3454412830501995E-5 12-13 0.0 0.0 0.0 2.3454412830501995E-4 4.690882566100399E-5 14-15 0.0 4.690882566100399E-5 0.0 2.3454412830501995E-4 4.690882566100399E-5 16-17 0.0 4.690882566100399E-5 0.0 2.579985411355219E-4 4.690882566100399E-5 18-19 0.0 4.690882566100399E-5 0.0 3.518161924575299E-4 4.690882566100399E-5 20-21 0.0 4.690882566100399E-5 0.0 5.159970822710438E-4 4.690882566100399E-5 22-23 0.0 4.690882566100399E-5 0.0 6.567235592540558E-4 4.690882566100399E-5 24-25 0.0 4.690882566100399E-5 0.0 8.443588618980718E-4 4.690882566100399E-5 26-27 0.0 4.690882566100399E-5 0.0 0.0011258118158640957 4.690882566100399E-5 28-29 0.0 4.690882566100399E-5 0.0 0.0015479912468131316 4.690882566100399E-5 30-31 0.0 4.690882566100399E-5 0.0 0.003752706052880319 4.690882566100399E-5 32-33 0.0 4.690882566100399E-5 0.0 0.008818859224268751 4.690882566100399E-5 34-35 0.0 4.690882566100399E-5 0.0 0.015831728660588847 4.690882566100399E-5 36-37 0.0 4.690882566100399E-5 0.0 0.026550395324128256 4.690882566100399E-5 38-39 0.0 4.690882566100399E-5 0.0 0.048480271320647625 4.690882566100399E-5 40-41 0.0 4.690882566100399E-5 0.0 0.07983882127502878 4.690882566100399E-5 42-43 0.0 4.690882566100399E-5 0.0 0.10988392411090184 4.690882566100399E-5 44-45 0.0 4.690882566100399E-5 0.0 0.14391627712796023 4.690882566100399E-5 46-47 0.0 4.690882566100399E-5 0.0 0.1866736717179654 4.690882566100399E-5 48-49 0.0 4.690882566100399E-5 0.0 0.23191723406800374 4.690882566100399E-5 50-51 0.0 4.690882566100399E-5 0.0 0.28433784674417567 4.690882566100399E-5 52-53 0.0 4.690882566100399E-5 0.0 0.3377669991720592 7.036323849150598E-5 54-55 0.0 4.690882566100399E-5 0.0 0.3961215782943482 9.381765132200798E-5 56-57 0.0 4.690882566100399E-5 0.0 0.4628259283842958 9.381765132200798E-5 58-59 0.0 4.690882566100399E-5 0.0 0.5391934965604104 9.381765132200798E-5 60-61 0.0 4.690882566100399E-5 0.0 0.6262562769872337 9.381765132200798E-5 62-63 0.0 4.690882566100399E-5 0.0 0.7219268269228514 9.381765132200798E-5 64-65 0.0 4.690882566100399E-5 0.0 0.8209279034804002 9.381765132200798E-5 66-67 0.0 4.690882566100399E-5 0.0 0.9342596262773859 9.381765132200798E-5 68-69 0.0 4.690882566100399E-5 0.0 1.0617343600111644 9.381765132200798E-5 70-71 0.0 4.690882566100399E-5 0.0 1.2052050032953452 9.381765132200798E-5 72-73 0.0 4.690882566100399E-5 0.0 1.3619508442415897 9.381765132200798E-5 74-75 0.0 4.690882566100399E-5 0.0 1.5248182869365956 9.381765132200798E-5 76-77 0.0 4.690882566100399E-5 0.0 1.7011016537706487 9.381765132200798E-5 78-79 0.0 4.690882566100399E-5 0.0 1.8984470833264924 9.381765132200798E-5 80-81 0.0 4.690882566100399E-5 0.0 2.118684019804906 9.381765132200798E-5 82-83 0.0 4.690882566100399E-5 0.0 2.367511885523702 9.381765132200798E-5 84-85 0.0 4.690882566100399E-5 0.0 2.627246053208681 9.381765132200798E-5 86-87 0.0 4.690882566100399E-5 0.0 2.8954003550998104 9.381765132200798E-5 88-89 0.0 4.690882566100399E-5 0.0 3.187337431601069 9.381765132200798E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2810 0.0 37.870007 1 TCAACGC 2875 0.0 35.192043 4 ATCAACG 2885 0.0 35.07006 3 CAACGCA 3005 0.0 33.827667 5 AACGCAG 3345 0.0 30.531282 6 TATCAAC 3505 0.0 30.224585 2 GGTATCA 1615 0.0 30.00415 1 ACGCAGA 3890 0.0 25.88743 7 CGCAGAG 3935 0.0 25.712103 8 GTACATG 5565 0.0 25.183176 1 TACATGG 5710 0.0 23.627932 2 ACATGGG 5575 0.0 22.83456 3 GCAGAGT 5330 0.0 18.626095 9 CATGGGG 3685 0.0 18.30432 4 GTATATA 1755 0.0 17.595026 1 AGAGTAC 4490 0.0 17.085148 10-11 GTGTAGG 1115 0.0 17.042715 1 GTATAAC 715 0.0 16.61069 1 GTACCTG 1610 0.0 16.524023 1 GTAGGAC 610 0.0 16.352802 3 >>END_MODULE