FastQCFastQC Report
Fri 10 Feb 2017
SRR2568664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131795
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.037.27471
TCAACGC29750.034.1763534
ATCAACG30150.033.880523
CAACGCA30400.033.445615
CCGATCG1001.3005192E-633.2479259
AACGCAG33650.030.2153476
TATCAAC35900.029.2865182
GGTATCA17400.028.4403951
GTACATG50500.026.5710811
TACCGTA1457.8794255E-726.2120237
ACGCAGA39600.025.4342677
TTTACGC950.001225165825.0060633
CGCAGAG40100.024.9922058
TACATGG57150.022.4759182
ACATGGG54050.022.2394353
GTACCGG2257.0280294E-821.1479851
GGACCGT2201.3427289E-619.4365316
ACCGTAT2451.7589264E-719.3865438
CATGGGG32350.018.7989964
GCAGAGT54600.018.0071169
Produced by FastQC (version 0.11.5)