FastQCFastQC Report
Fri 10 Feb 2017
SRR2568663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000521
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.032.4086231
TCAACGC30900.029.0533284
CAACGCA32500.028.061475
ATCAACG32200.027.8794363
GTATCAA35350.027.5477391
ACATGGG74250.026.9316963
GTACATG83650.026.2359961
AACGCAG36000.025.5971586
TACATGG88250.023.5765842
TATCAAC41050.022.3339562
ACGCAGA41650.021.7826587
CGCAGAG42350.021.1982968
CATGGGG44300.021.0157494
CGTCCGA2450.019.38770710-11
GAGTACA61450.018.9393771
ATACCGA2101.957549E-518.0951946
GGTCGCC6700.018.0841994-95
CATGGGC22850.016.8380324
TATACTG20400.016.7646665
CTTACAC19650.016.6787913
Produced by FastQC (version 0.11.5)