FastQCFastQC Report
Fri 10 Feb 2017
SRR2568663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000521
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT34280.11424682580125252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30050.033.2061544
CAACGCA31650.031.9778825
ATCAACG32350.030.8452853
GTATCAA36750.029.7739121
AACGCAG35400.028.4561656
GGTATCA21800.027.7148571
GTACATG76300.025.9378621
ACATGGG70050.025.369233
TATCAAC41550.025.1830482
ACGCAGA41400.023.872197
CGCAGAG40800.023.7487138
TACATGG79600.023.6015592
GGACCGT3252.1827873E-1120.4686156
TAATACT27800.019.6560764
CATGGGG38200.018.907064
GTATTAC9850.018.836221
TTACGCG650.007569120718.2682480-81
GAGTACA56800.018.1750981
TTAATAC25600.018.004273
AAGACCG5350.017.7631515
Produced by FastQC (version 0.11.5)