FastQCFastQC Report
Fri 10 Feb 2017
SRR2568662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090155
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC39520.12789002493402435No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC34210.11070642087532825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.037.4573861
TCAACGC38100.030.7932724
CAACGCA39750.029.6345545
ATCAACG39950.029.36733
AACGCAG41800.028.2948196
GTACATG91550.028.2266641
GTATCAA46400.026.720311
ACATGGG87800.026.5626143
CGCAGAG47750.024.4706548
ACGCAGA48750.023.9686937
TATCAAC50150.023.9645122
CATGGGG40950.023.5464764
GTATAGG19300.023.3821951
TACATGG108850.023.0858672
ACCGTCG1100.002878829121.5904248
CTTATCG1404.8550242E-420.3566849
GTATTAC10700.019.08991
CATGGGC23200.019.040524
GTCCGAC2001.8189894E-1218.99803512-13
CATGGGA38150.018.5513534
Produced by FastQC (version 0.11.5)