FastQCFastQC Report
Fri 10 Feb 2017
SRR2568662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090155
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC49120.15895642775200597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC34000.037.0369954
CAACGCA35300.035.8076445
ATCAACG37150.034.280313
AACGCAG37250.034.060726
GGTATCA24250.032.7680361
GTATCAA44350.031.435451
CGCAGAG42900.029.2288448
ACGCAGA44000.028.833167
TATCAAC47650.027.5549952
GTACATG85600.026.0146371
ACATGGG80750.025.9516453
ATAATAC19350.025.0488643
TAATACT38200.022.5156274
CATGGGG37550.021.5133384
GCAGAGT59750.021.2245459
TACATGG103950.020.9144232
TTAATAC37450.020.6825733
TAATACA25250.020.325034
GTATTAC10500.019.9392621
ACCGCTC2151.0806853E-619.8824128
Produced by FastQC (version 0.11.5)