FastQCFastQC Report
Fri 10 Feb 2017
SRR2568661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.044.1065031
GTATCAA33300.042.6495321
TCAACGC37700.036.7899174
ATCAACG38850.035.5786363
CAACGCA39250.035.3370675
TATCAAC41700.033.260912
AACGCAG42200.033.205376
TATACCG1401.7074854E-830.535275
ACGCAGA48300.028.71677
CGCAGAG48450.028.6277948
CGTAATA856.409264E-427.9407712
GTACATG62650.027.2940981
ATACCGA1405.8088335E-727.1431836
TACATGG65650.025.5403772
TAAACCG950.001226951124.9996385
ACATGGG64550.024.8718323
TAACGCA1000.001654177923.7496574
GCAGAGT58550.023.2837989
CATGGGG39850.023.2430764
GTATATA16350.020.9171281
Produced by FastQC (version 0.11.5)