FastQCFastQC Report
Fri 10 Feb 2017
SRR2568661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.038.846431
GTATCAA32900.038.604981
TCAACGC37450.032.7341844
CAACGCA37550.032.520475
ATCAACG39100.031.4743423
AACGCAG40800.030.1629126
TATCAAC42300.029.4536692
ACGCAGA47900.025.5921257
CGCAGAG47450.025.425558
GTACATG62100.025.2784421
TACATGG63400.023.9265372
ACATGGG61000.023.3681873
GCGCTAA1100.002878845221.5898769
GCAGAGT57200.020.5103829
CATGGGG32900.019.3527434
AGAGTAC49000.018.8536910-11
TACCGTA2051.5862288E-518.5420727
GGCGCTA1300.007566974518.2683568
GTACTAT7400.017.9992451
GAGTACA45150.017.5948941
Produced by FastQC (version 0.11.5)