FastQCFastQC Report
Fri 10 Feb 2017
SRR2568660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4512199
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC58420.12947124007606933No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC52570.11650638635397066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.039.1098251
GGTATCA43300.036.9716841
TCAACGC78350.036.4964834
GTACATG230600.036.0088581
CAACGCA80500.035.5217365
ATCAACG80650.035.455673
AACGCAG84250.034.0537836
ACATGGG241500.032.453413
TATCAAC91050.031.9821592
TACATGG258800.031.387672
CATGGGG115350.029.23714
ACGCAGA104250.027.429567
CGCAGAG105850.026.7008138
CATGGGA122950.026.3481064
GAGTACA147000.023.5902631
AGTACAT143000.023.3864272
ATGGGGG54400.022.8768355
ATGGGAT64650.022.7764855
GTATACG3850.022.2095131
CCCTATA10700.021.754022
Produced by FastQC (version 0.11.5)