FastQCFastQC Report
Fri 10 Feb 2017
SRR2568660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4512199
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC71800.15912418756353608No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC45500.10083775117187874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76500.039.9185941
TCAACGC78350.036.5083274
ATCAACG79850.035.822513
CAACGCA80050.035.733015
GTACATG218700.035.2344781
GGTATCA42600.034.7258151
AACGCAG84300.034.156986
TATCAAC91550.032.518532
ACATGGG232750.031.5612533
TACATGG249350.030.4014192
ACGCAGA101600.028.2457797
CATGGGG107050.027.6969764
CGCAGAG102750.027.5970888
CATGGGA120500.026.6953744
TGTACCG5000.023.7577025
GAGTACA141200.023.6148381
AGTACAT137500.022.9311472
TTAATAC42250.022.0426483
ATGGGGG47600.021.7612555
CCTATAC14400.021.1179563
Produced by FastQC (version 0.11.5)