FastQCFastQC Report
Fri 10 Feb 2017
SRR2568659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821189
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.033.485751
TCAACGC23100.031.0507074
GTATCAA23750.030.9992221
CAACGCA24000.030.4800745
ATCAACG23600.030.1899193
GTACATG54200.029.1828461
AACGCAG26650.027.9839486
TACATGG56050.027.9653992
ACATGGG55550.027.276543
TATCAAC27300.026.0982442
ACGCAGA31500.023.0721157
CATGGGG30500.022.582644
CGCAGAG32300.022.3536078
GACCCGT1200.00476372919.7922556
GTGTAGG12000.019.7911681
GTCTAGG7450.019.7646031
GTACACG3655.456968E-1219.5200581
GTCGTGA1951.0334134E-519.486691
TATACTG10500.019.0005665
ATGGGGG18050.018.1584365
Produced by FastQC (version 0.11.5)