FastQCFastQC Report
Fri 10 Feb 2017
SRR2568659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821189
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT41180.22611601541630222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.032.4945451
CAACGCA24150.031.8746595
TCAACGC24100.031.7436224
ATCAACG24650.031.0353493
AACGCAG26400.029.3380556
TATACCG2450.029.0919485
GGTATCA15300.028.6067961
TATCAAC27900.028.127632
CGTAACA3450.027.5717492
CGCAGAG31050.024.934168
GTACATG49750.024.6717191
ACGCAGA31450.024.4747457
ATACCGT3200.023.7584276
ATAACGC1201.7072407E-423.7584253
TACATGG50500.022.320832
AGGACCG2457.496055E-921.3340955
TATAGGG4200.020.38342
ACATGGG51800.020.3643653
TACCGTC2108.588613E-720.3632457
TATCCCG1656.308078E-520.1586635
Produced by FastQC (version 0.11.5)