FastQCFastQC Report
Fri 10 Feb 2017
SRR2568658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752977
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC162670.9279642573747402No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA92030.5249926268285323No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC90940.5187746330955854No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA86620.4941308414200528No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT76610.4370279815422564No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT75250.4292697508295888No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT69790.39812273635079065No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC67130.3829485498098378No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA63130.3601302241843447No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA61000.3479794657887696No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG59340.33850986065419003No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT57140.32595978156016875No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT53360.3043964638440778No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT49300.28123586333420236No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG47280.2697126088933283No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC45140.2575048046836895No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG42680.24347153442401126No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG41320.2357133037113436No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT40940.2335455627769218No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG40110.22881076020963195No Hit
GAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCTTTATT39870.22744166067210236No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC39310.22424709508453333No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT37550.21420703180931636No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT36900.21049905389517376No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC36760.20970041249828147No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC36270.2069051676091586No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC35990.20530788481537407No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT35880.204680380860673No Hit
CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA35330.2015428610871677No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC35290.2013146778309128No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA34730.19812011224334378No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT34590.1973214708464515No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG34060.19429804270107368No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA33560.19144575199788702No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT33140.18904982780721025No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG31850.1816909177929887No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT31810.18146273453673378No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT31680.18072113895390526No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC31560.18003658918514048No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG30840.17592929057255172No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA29970.17096630474900698No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT29940.17079516730681576No Hit
TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT29830.17016766335211472No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG29280.16703014357860943No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT29030.1656039982270161No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC28630.16332216566446678No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT28180.16075510403159882No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC27320.15584916402211782No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC27210.15522166006741675No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT26910.15351028564550478No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA26450.15088617819857306No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT26330.15020162842980825No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT26030.14849025400789628No Hit
GTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTG25830.14734933772662162No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA25820.1472922919125579No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC25720.14672183377192055No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA25110.14324203911403288No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA24200.1380508700342332No Hit
ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG24190.13799382422016945No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT24160.13782268677797826No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC23840.13599722072793882No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC23670.13502744188885538No Hit
CTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGG23520.13417175467789938No Hit
ATCCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACA23450.13377243397945324No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC23420.13360129653726202No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT23370.13331606746694338No Hit
GGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATG23160.13211810537160498No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT23090.13171878467315884No Hit
GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT23030.13137650978877646No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT22510.12841012745746236No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG21750.12407464558861866No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA21580.12310486674953522No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC21340.12173576721200563No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC21260.12127940069949576No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC21130.12053780511666724No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG21060.12013848441822111No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC20930.1193968888353926No Hit
ATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCGTGAAGCACG20880.11911165976507393No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA20790.11859824743850034No Hit
TGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCGTGAAGCA20580.11740028534316194No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG20490.11688687301658834No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG20470.1167727813884609No Hit
TAATTATAGTGGCTGATGTAAAGTAAGCTCGTGTGTCTACATCTAATCCT20380.1162593690618873No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT20340.11603118580563238No Hit
TAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATC20240.11546072766499503No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC20090.11460504045403903No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC20000.11409162812746544No Hit
GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA19710.1124372995196172No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC19700.11238025370555348No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA19640.11203797882117106No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC19030.10855818416328337No Hit
GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT18960.10815886346483723No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC18890.10775954276639112No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT18710.10673271811324392No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC18140.10348110671161116No Hit
CAGCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGT18010.10273951112878263No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA17970.1025113278725277No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA17820.1016556406615717No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA17720.10108518252093439No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG17600.1004006327521696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT14250.060.006031
ACATATA15650.054.6365242
TAACTTT9100.042.2784426
TCATAAC3850.041.9510772
CATATAG20600.041.5054743
ATTGAAC3450.041.3026128
CATTCCG2000.040.3733029
GGTATCA20650.040.028241
CATAACC4300.038.6633723
CGTCTGA250.001613093538.0152194-95
ATCGCTA250.001616828537.9973262-63
ACTTAAT46100.037.9213871
CTTAATA46850.037.211862
ATGTATT1054.6558853E-836.1941151
ACTTCAG48600.035.7701038
ACCCTTG11700.034.9130637
ACTAAGC550.002579803334.5459753
GCCGTCC550.002580525434.5440038
AACTTTA11150.034.505287
GGCATTC2350.034.3602567
Produced by FastQC (version 0.11.5)