FastQCFastQC Report
Fri 10 Feb 2017
SRR2568658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752977
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC144360.8235133718240455No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT100530.5734815687827051No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC85300.4866007939636401No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA84010.47924188394941863No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT80720.46047381112245056No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA76460.43617229433130045No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT74880.4271590557092307No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC73160.41734717569026863No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG63600.36281137744534014No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA63080.359844995114026No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA58140.33166436296654206No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG50410.2875679486952767No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT45010.256763209100861No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA44700.25499478886488525No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC44430.25345455188516447No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT43670.2491190700163208No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC43360.2473506497803451No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT41230.23519989138477004No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG40330.2300657681190341No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT40030.2283543936971221No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT39090.2229920871751312No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC37580.21437816925150757No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG37250.21249565738740442No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC36660.20912995435764417No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT36540.20844540458887936No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT35850.20450924341848178No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT35470.20234150248406No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG33970.19378463037450008No Hit
CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA33540.19133166036975957No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT32230.18385865872741058No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC31990.182489559189881No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT31550.17997954337107674No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA30860.1760433822006792No Hit
GAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCTTTATT30840.17592929057255172No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT29420.16782878497550166No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG28920.16497649427231503No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG28740.16394966961916785No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG28390.16195306612693722No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT28380.16189602031287348No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT28160.16064101240347134No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT28050.1600135084487703No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC27510.15693303448932872No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT27500.15687598867526498No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG27270.15556393495179913No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC27230.1553357516955442No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT27010.1540807437861421No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT26850.15316801076112235No Hit
TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT26620.1518559570376565No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC26510.15122845308295546No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC26490.15111436145482798No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA26280.1499163993594896No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA25830.14734933772662162No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA25580.14592319237502832No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC25020.1427286267874593No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA24940.1422722602749494No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT24830.14164475632024834No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA24640.14056088585303744No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC23550.13434289212009057No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA22960.13097718909033032No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC22930.13080605164813913No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG22920.1307490058340754No Hit
ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG22920.1307490058340754No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC22810.13012150187937432No Hit
GGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATG22130.12624238652304054No Hit
ATCCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACA22130.12624238652304054No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC22080.12595715745272185No Hit
CTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGG21760.12413169140268242No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG21670.12361827907610881No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA21630.12339009581985387No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC21620.12333305000579015No Hit
GTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTG21520.12276259186515283No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG21500.12264850023702535No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT20920.11933984302132886No Hit
TAATTATAGTGGCTGATGTAAAGTAAGCTCGTGTGTCTACATCTAATCCT20500.11694391883065207No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC20120.11477617789623024No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA20110.11471913208216651No Hit
GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT20090.11460504045403903No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC20060.11443390301184786No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA19950.11380639905714679No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA19940.11374935324308307No Hit
GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT19850.11323594091650944No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT19740.1126084369618084No Hit
ATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACG19600.11180979556491613No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC19550.11152456649459748No Hit
ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA19160.1092997797461119No Hit
AGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACAT19010.10844409253515591No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA19000.10838704672109217No Hit
GGCTACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGG18710.10673271811324392No Hit
ATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCGTGAAGCACG18690.10661862648511647No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT18530.10570589346009673No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC18270.10422270229443968No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC18060.1030247401991013No Hit
ACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTC17870.10194086973189039No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT17850.10182677810376291No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC17830.10171268647563546No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT17800.10154154903344426No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC17780.10142745740531678No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG17760.10131336577718932No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC17710.10102813670687066No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC17690.10091404507874319No Hit
CCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCGTG17690.10091404507874319No Hit
TCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACA17650.10068586182248826No Hit
CAGCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGT17630.1005717701943608No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT17530.10000131205372347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT14200.059.9675831
TAGCGGG250.00501599357.0164227
ACATATA16050.053.635972
ATGTATT654.368667E-851.2314381
GTTACAC502.8213706E-547.517753
GGTATCA20050.044.1316761
CATATAG20200.042.577783
TAACTTT8400.041.2951856
ACCCTTG13450.040.6250767
AGTTACA608.229882E-539.6343152
CAGTTCA608.295195E-539.581189
GTATCAA42400.038.4839941
GGGGAGC3100.038.3174867
AACCCTT14350.038.0804256
CCTTGGC14350.038.0641339
CCCTTGG14600.037.0870258
TGGGGAG4950.035.518326
CATTCCG2450.034.896069
GGATATG33650.034.2122881
GAGTATG702.0355656E-433.9800341
Produced by FastQC (version 0.11.5)