FastQCFastQC Report
Fri 10 Feb 2017
SRR2568657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679742
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.21552294841278014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.19374998161066992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.10283313374780431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.059.850191
GTATCAA17500.038.8144071
TCAACGC22450.030.0446364
ATCAACG22500.029.977873
CAACGCA22900.029.6616635
TATCAAC23950.028.1629282
AACGCAG24950.027.6052956
ACATGGG22750.026.934153
GTACATG24150.026.7495631
TACATGG23650.026.5117112
ACGCAGA27000.025.1574867
CGCAGAG27450.024.5720238
CATGGGG14700.023.588514
GCAGAGT29700.022.0707749
AGAGTAC26650.021.7441110-11
ATAATAC4450.021.3483813
CAGAGTA29000.020.39155410-11
TACCTGG4200.020.3572082
GCATCGC600.00476588219.79027236-37
TGGTATC4850.019.5876882
Produced by FastQC (version 0.11.5)