##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568657_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679742 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790901547940248 33.0 31.0 34.0 30.0 34.0 2 31.928692062576683 34.0 31.0 34.0 30.0 34.0 3 31.949779475153807 34.0 31.0 34.0 30.0 34.0 4 35.322803357744554 37.0 35.0 37.0 33.0 37.0 5 35.37222063665332 37.0 35.0 37.0 33.0 37.0 6 35.484279329510315 37.0 35.0 37.0 33.0 37.0 7 35.467359674700106 37.0 35.0 37.0 33.0 37.0 8 35.49168949395506 37.0 35.0 37.0 33.0 37.0 9 37.24907979792333 39.0 38.0 39.0 34.0 39.0 10-11 37.22276319544768 39.0 37.5 39.0 34.0 39.0 12-13 37.19328657049292 39.0 37.0 39.0 34.0 39.0 14-15 38.53791585631019 41.0 38.0 41.0 34.0 41.0 16-17 38.504540693380726 41.0 38.0 41.0 33.5 41.0 18-19 38.50925718875691 41.0 38.5 41.0 33.5 41.0 20-21 38.512106063771256 41.0 39.0 41.0 34.0 41.0 22-23 38.44126006631928 40.0 38.5 41.0 34.0 41.0 24-25 38.376369269516964 40.0 38.0 41.0 33.0 41.0 26-27 38.27019957572138 40.0 38.0 41.0 33.0 41.0 28-29 38.134481906370354 40.0 38.0 41.0 33.0 41.0 30-31 38.021740601581186 40.0 38.0 41.0 33.0 41.0 32-33 37.875365947668385 40.0 38.0 41.0 32.5 41.0 34-35 37.71801139255776 40.0 38.0 41.0 32.0 41.0 36-37 37.58653136042793 40.0 37.0 41.0 31.0 41.0 38-39 37.46585984093965 40.0 37.0 41.0 31.0 41.0 40-41 37.31999641040277 40.0 37.0 41.0 30.5 41.0 42-43 37.13619946979884 40.0 37.0 41.0 30.0 41.0 44-45 36.92593734093229 40.0 36.5 41.0 30.0 41.0 46-47 36.931994786257135 40.0 36.5 41.0 30.0 41.0 48-49 37.0490546413198 40.0 37.0 41.0 30.0 41.0 50-51 35.941710972692576 39.0 35.5 40.0 28.5 40.5 52-53 36.08668141736129 39.0 35.5 40.0 28.5 40.5 54-55 36.689675641640505 40.0 36.0 41.0 29.0 41.0 56-57 36.66825501440252 40.0 36.0 41.0 29.0 41.0 58-59 36.520218700624646 39.5 35.5 41.0 28.5 41.0 60-61 36.32464891091031 39.0 35.0 41.0 28.0 41.0 62-63 36.07038037961462 39.0 35.0 41.0 28.0 41.0 64-65 35.89716392395938 39.0 35.0 41.0 28.0 41.0 66-67 35.78047479779093 38.5 35.0 41.0 28.0 41.0 68-69 35.49156518208379 37.5 35.0 40.0 28.0 41.0 70-71 35.13849592933789 37.0 34.5 40.0 27.5 41.0 72-73 34.78294191031303 36.5 34.0 39.0 26.5 41.0 74-75 34.436952549643834 36.0 34.0 39.0 26.0 41.0 76-77 34.07758531913579 35.5 34.0 38.5 26.0 40.0 78-79 33.72037846712429 35.0 34.0 37.0 26.0 39.0 80-81 33.364748684059535 35.0 34.0 37.0 26.0 39.0 82-83 33.04856474956675 35.0 33.0 36.5 26.0 39.0 84-85 32.75363740948772 35.0 33.0 36.0 25.0 37.0 86-87 32.509400625531455 35.0 33.0 36.0 25.0 37.0 88-89 32.28845503146782 35.0 33.0 35.0 25.0 37.0 90-91 32.10530171741631 35.0 33.0 35.0 24.0 36.0 92-93 31.95385381512397 35.0 33.0 35.0 24.0 36.0 94-95 31.8126590677051 35.0 32.0 35.0 24.0 36.0 96-97 31.706124529600938 35.0 32.0 35.0 23.5 36.0 98-99 31.5586288621271 35.0 32.0 35.0 23.0 35.0 100-101 30.289919557714544 34.0 29.5 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10652306130501543 1101 2 0.02611209115748636 1101 3 0.20942922930471397 1101 4 0.01389382207879919 1101 5 -0.08095948304549694 1101 6 0.003011339137749758 1101 7 -0.06673745319671553 1101 8 0.09311181057002926 1101 9 0.3534944302851457 1101 10-11 -0.022083068958615115 1101 12-13 0.14060793381290893 1101 14-15 0.10206672213848833 1101 16-17 0.22321694198427622 1101 18-19 0.24230868289240703 1101 20-21 -0.05479773979382685 1101 22-23 0.10169663446404087 1101 24-25 -0.0012735408421278294 1101 26-27 0.06599341774565204 1101 28-29 0.08858569365760616 1101 30-31 0.14516859129343374 1101 32-33 0.16577047146832768 1101 34-35 0.004860499309273791 1101 36-37 -0.10207690289327331 1101 38-39 0.03457974487449178 1101 40-41 -0.016376898234348403 1101 42-43 -0.05465991532407344 1101 44-45 0.1631880592163668 1101 46-47 0.022026390618094638 1101 48-49 0.22653709980579606 1101 50-51 0.16253465583431392 1101 52-53 -0.07482037186309043 1101 54-55 -0.0046551601410769194 1101 56-57 0.17255067104259325 1101 58-59 -0.013542349814706256 1101 60-61 0.2352731442560625 1101 62-63 0.34683635754765163 1101 64-65 0.08355938235984439 1101 66-67 0.013517929760446634 1101 68-69 0.1988270172185409 1101 70-71 0.31920690464477275 1101 72-73 0.23659594677059914 1101 74-75 0.2452965111236196 1101 76-77 0.17842784524069089 1101 78-79 0.03372633591611418 1101 80-81 0.08291562777673533 1101 82-83 0.07447808542703171 1101 84-85 -0.15830575778545608 1101 86-87 0.07770270627442244 1101 88-89 0.10799731658885037 1101 90-91 -0.12071720278913745 1101 92-93 0.09000000088158089 1101 94-95 -0.1910027584067393 1101 96-97 -0.04477376700818425 1101 98-99 0.05794247048882184 1101 100-101 -0.06894596315998314 1102 1 0.1693195639205598 1102 2 0.09645082417517514 1102 3 0.27181595535385483 1102 4 0.29161227982029914 1102 5 0.32372648760981804 1102 6 0.10616966275352269 1102 7 0.29955596555747377 1102 8 0.10742589142947878 1102 9 0.18428714890679743 1102 10-11 0.3399252577012106 1102 12-13 0.3517845311219574 1102 14-15 0.344895632226482 1102 16-17 0.2800939294006213 1102 18-19 0.24029609455629242 1102 20-21 0.24867927291159475 1102 22-23 0.1657552489040981 1102 24-25 0.236594731212044 1102 26-27 0.34745723330404843 1102 28-29 0.5911942142002928 1102 30-31 0.3856102601846061 1102 32-33 0.3154185319372971 1102 34-35 0.43407218285872773 1102 36-37 0.6188219361511713 1102 38-39 0.4916538933587944 1102 40-41 0.4341349712365954 1102 42-43 0.19818627562712976 1102 44-45 0.390781476565607 1102 46-47 0.6619346474988248 1102 48-49 0.7428321576662427 1102 50-51 0.5659596854553044 1102 52-53 0.5680476426172376 1102 54-55 0.6547830478942913 1102 56-57 0.7529687368035383 1102 58-59 0.5696914663525021 1102 60-61 0.5554386003256653 1102 62-63 0.985272931159912 1102 64-65 0.8380891696038475 1102 66-67 0.6995354166827212 1102 68-69 0.5493639415995446 1102 70-71 0.8467169617325041 1102 72-73 0.6531478759986697 1102 74-75 0.6432480966076994 1102 76-77 0.5158636273987867 1102 78-79 0.3581897627721631 1102 80-81 0.4040282531098214 1102 82-83 0.5138135917469526 1102 84-85 0.7657649860409705 1102 86-87 0.7609618115097305 1102 88-89 0.4689308578545237 1102 90-91 0.5627128378138693 1102 92-93 0.6724037268042835 1102 94-95 0.33317171743048846 1102 96-97 0.4541151531017782 1102 98-99 0.41852828706814904 1102 100-101 0.3037481655613732 1103 1 0.26923304872488174 1103 2 0.21523803843213685 1103 3 0.23425124245013862 1103 4 0.17126594144873053 1103 5 0.2992477763623782 1103 6 0.19170173120693335 1103 7 0.30156385833414845 1103 8 0.1822060179174727 1103 9 0.18815026058542372 1103 10-11 0.2903640794290183 1103 12-13 0.3317969763559887 1103 14-15 0.4159560679484713 1103 16-17 0.3852145186292475 1103 18-19 0.36102296099441133 1103 20-21 0.34049988573526946 1103 22-23 0.33112881411665995 1103 24-25 0.1613993780496159 1103 26-27 0.2961275826496319 1103 28-29 0.2567366459712588 1103 30-31 0.4825223154062783 1103 32-33 0.42894543723785716 1103 34-35 0.2887266541213478 1103 36-37 0.3521480984388745 1103 38-39 0.19329675678044111 1103 40-41 0.20764443470358884 1103 42-43 0.3090182557147543 1103 44-45 0.2679819466160751 1103 46-47 0.1891973437194565 1103 48-49 0.39258209636537345 1103 50-51 0.4094631807617404 1103 52-53 0.4614662419963693 1103 54-55 0.5977362232795613 1103 56-57 0.5761018360369263 1103 58-59 0.6702387790609805 1103 60-61 0.5909549387435007 1103 62-63 0.609771536275872 1103 64-65 0.618657486536307 1103 66-67 0.6008757082435565 1103 68-69 0.6927894807002488 1103 70-71 0.6035225153603605 1103 72-73 0.43084661805848157 1103 74-75 0.5646349305679195 1103 76-77 0.6133204507643697 1103 78-79 0.6148044740566121 1103 80-81 0.5323275124102054 1103 82-83 0.6298538328791423 1103 84-85 0.3382723537120427 1103 86-87 0.41327250747127664 1103 88-89 0.503465225669018 1103 90-91 0.4960845709399351 1103 92-93 0.5478461340549003 1103 94-95 0.404278417962999 1103 96-97 0.45550888051876726 1103 98-99 0.7244855753950716 1103 100-101 0.5693833582774417 1104 1 0.20198444443800057 1104 2 0.16628655257224167 1104 3 0.12981709199427272 1104 4 0.25828435906037583 1104 5 0.0748157680081789 1104 6 0.17209480686328504 1104 7 0.21629705510849817 1104 8 0.2804793947176165 1104 9 -0.032256381714830695 1104 10-11 0.18269000019803627 1104 12-13 0.11440935859618406 1104 14-15 0.10096448016023629 1104 16-17 0.04466051758497258 1104 18-19 0.22548594056857496 1104 20-21 0.20235441025564427 1104 22-23 0.13762339861587947 1104 24-25 0.13979762152624886 1104 26-27 0.15365327671288753 1104 28-29 0.17951388078576969 1104 30-31 0.09239762723826317 1104 32-33 0.12483505652391358 1104 34-35 0.013873812043328826 1104 36-37 0.0892151596092603 1104 38-39 -0.2616410107033431 1104 40-41 -0.055655562172511 1104 42-43 -0.1246669624449126 1104 44-45 0.0358316781839676 1104 46-47 -0.012634376343555687 1104 48-49 -0.162217773323448 1104 50-51 -0.10000334666831634 1104 52-53 0.04670180623933362 1104 54-55 -0.0801497008811154 1104 56-57 -0.28196942454528084 1104 58-59 -0.19293336112172454 1104 60-61 -0.10586961866705025 1104 62-63 0.08052069170766174 1104 64-65 0.0880756381703236 1104 66-67 0.11290717928725513 1104 68-69 0.0628074208017253 1104 70-71 -0.16930425555525375 1104 72-73 -0.019120804145302372 1104 74-75 0.08601689853457373 1104 76-77 -0.06703670006190521 1104 78-79 -0.08061432913333277 1104 80-81 -0.2125247495152749 1104 82-83 -0.386236474557073 1104 84-85 -0.1405230711158083 1104 86-87 -0.17777462322817428 1104 88-89 -0.15488852457085756 1104 90-91 -0.21573023544531367 1104 92-93 -0.039349676351250196 1104 94-95 0.004797654482260327 1104 96-97 -0.06271795019718596 1104 98-99 0.14950309153893215 1104 100-101 -0.18908017467299487 1105 1 0.13475780576993657 1105 2 0.01916890845444641 1105 3 0.42390738747949896 1105 4 0.024816032476699945 1105 5 0.1819704102760653 1105 6 0.19058888215952408 1105 7 0.20610756458302149 1105 8 0.37994442146628415 1105 9 0.320745968167671 1105 10-11 0.37627782080700456 1105 12-13 0.36959409935914067 1105 14-15 0.4765806993492703 1105 16-17 0.556359932614221 1105 18-19 0.5752284534429322 1105 20-21 0.3632363661578495 1105 22-23 0.5451830206347736 1105 24-25 0.5577967215712434 1105 26-27 0.5670576064963981 1105 28-29 0.611507281115756 1105 30-31 0.5923251308630881 1105 32-33 0.5127206622436304 1105 34-35 0.6008269643857105 1105 36-37 0.7564417982773293 1105 38-39 0.5500559044509004 1105 40-41 0.40736878661005704 1105 42-43 0.3948845599789692 1105 44-45 0.4660626666345422 1105 46-47 0.5262636787536863 1105 48-49 0.6792676524052723 1105 50-51 0.6067413160985424 1105 52-53 0.5167933016645563 1105 54-55 0.26200069159926187 1105 56-57 0.38799957700464205 1105 58-59 0.24001749133565653 1105 60-61 0.21076954937454673 1105 62-63 0.2529995677638581 1105 64-65 0.41553176536396563 1105 66-67 0.42064929218160074 1105 68-69 0.5491281047675258 1105 70-71 0.49976729086741756 1105 72-73 0.5691847805754691 1105 74-75 0.3251249431323444 1105 76-77 0.4080395461257922 1105 78-79 0.47687529638538706 1105 80-81 0.48633530748187326 1105 82-83 0.25837379492946155 1105 84-85 0.35429968774625564 1105 86-87 0.48169990304551646 1105 88-89 0.3568383890834639 1105 90-91 0.21625816513466134 1105 92-93 0.2879689577170481 1105 94-95 0.1953756255837078 1105 96-97 0.34722405270266776 1105 98-99 0.29293092014750144 1105 100-101 -0.003256965833436709 1106 1 0.29806350909790424 1106 2 0.16486976209789717 1106 3 0.27458164208793434 1106 4 0.21986737480009566 1106 5 0.2946142306397945 1106 6 0.3152703123215943 1106 7 0.22054527335451724 1106 8 0.20926521820016575 1106 9 0.29339226286023745 1106 10-11 0.3116545373726325 1106 12-13 0.2654310410886964 1106 14-15 0.3785972773524904 1106 16-17 0.42222656628983657 1106 18-19 0.34286423223793605 1106 20-21 0.37318316808782015 1106 22-23 0.3792421570940121 1106 24-25 0.5631165285735946 1106 26-27 0.31559156665970534 1106 28-29 0.25660192419381644 1106 30-31 0.14949449867207676 1106 32-33 0.31881369700794693 1106 34-35 0.3954329275182076 1106 36-37 0.4818222263362273 1106 38-39 0.56472248863993 1106 40-41 0.5074368306531056 1106 42-43 0.45857378427687223 1106 44-45 0.7311808158365665 1106 46-47 0.6015884344137135 1106 48-49 0.4820450865262558 1106 50-51 0.5944748308826533 1106 52-53 0.6302041639681661 1106 54-55 0.4825314351350798 1106 56-57 0.7338488903783968 1106 58-59 0.6096945914222616 1106 60-61 0.41472228969373504 1106 62-63 0.40114382113512903 1106 64-65 0.3423906049037413 1106 66-67 0.39231204116094176 1106 68-69 0.4236981901318799 1106 70-71 0.4757477436695936 1106 72-73 0.4403897787197195 1106 74-75 0.6749764834020411 1106 76-77 0.725662953276597 1106 78-79 0.5824425362715715 1106 80-81 0.5438572671503081 1106 82-83 0.33128607997213777 1106 84-85 0.3445446463012374 1106 86-87 0.3138290630108713 1106 88-89 0.35926323134500393 1106 90-91 0.22063154135635443 1106 92-93 0.2929856594367095 1106 94-95 0.27238911067587424 1106 96-97 0.23003983892065705 1106 98-99 0.2423803819809791 1106 100-101 0.2693645855707487 1107 1 0.004531817069370447 1107 2 0.053067213539190305 1107 3 0.23209947787497853 1107 4 0.10645445055579472 1107 5 0.062478884852339434 1107 6 0.16770752622731777 1107 7 0.09509061543048603 1107 8 0.12259978869791865 1107 9 0.0040793015010081035 1107 10-11 0.1176620015979637 1107 12-13 0.1526449468167712 1107 14-15 0.19677843946226403 1107 16-17 0.09025823769896846 1107 18-19 0.029183255702818656 1107 20-21 0.09219116841773456 1107 22-23 0.055352512160197875 1107 24-25 0.15214700405712023 1107 26-27 0.147566081072668 1107 28-29 0.3369310099293088 1107 30-31 0.24995224950716022 1107 32-33 0.012296933430064882 1107 34-35 0.14079871579814096 1107 36-37 -0.01206102658142072 1107 38-39 -0.006441270690345391 1107 40-41 -0.06028658062158598 1107 42-43 0.020025802916819657 1107 44-45 0.2550457174819982 1107 46-47 0.20352356575934039 1107 48-49 0.07771398008888042 1107 50-51 0.164933406494022 1107 52-53 0.33967465759676685 1107 54-55 0.21072950515858935 1107 56-57 0.25678488773910146 1107 58-59 0.2186898077198336 1107 60-61 0.3953740691485592 1107 62-63 0.03972273160567141 1107 64-65 0.27202894050520854 1107 66-67 0.2268639814471456 1107 68-69 0.05661398047374888 1107 70-71 0.21756390103691103 1107 72-73 0.384721503739307 1107 74-75 0.3756899148837576 1107 76-77 0.12484745573030409 1107 78-79 0.162186030848666 1107 80-81 0.08916016623893341 1107 82-83 0.19509695877126632 1107 84-85 0.17972341655980983 1107 86-87 0.2342422759268743 1107 88-89 -3.6500074704548524E-4 1107 90-91 0.2351847188069769 1107 92-93 0.1324604831407754 1107 94-95 0.33873720750460734 1107 96-97 0.27095286626198956 1107 98-99 0.23346764331134295 1107 100-101 0.21114981382758202 1108 1 0.11903962331592055 1108 2 0.10742340219468716 1108 3 0.26894503931080393 1108 4 0.2827555564083468 1108 5 0.06784689361833784 1108 6 -0.04863896387595901 1108 7 0.13702636252208578 1108 8 -0.0035260748851086987 1108 9 0.004174499629684192 1108 10-11 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0.14977540921653087 2316 82-83 0.055819500018181145 2316 84-85 -0.1214320268403597 2316 86-87 -0.10777095752634125 2316 88-89 0.2394242773740487 2316 90-91 -0.013273696490315956 2316 92-93 0.1256475397902861 2316 94-95 0.1507574285920299 2316 96-97 -0.002369604777410217 2316 98-99 0.15480625545459503 2316 100-101 0.2521466942297508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 13.0 7 297.0 8 439.0 9 257.0 10 292.0 11 421.0 12 695.0 13 1063.0 14 1459.0 15 1660.0 16 2081.0 17 2376.0 18 2804.0 19 3455.0 20 4042.0 21 4991.0 22 5761.0 23 5859.0 24 5924.0 25 5739.0 26 5952.0 27 6465.0 28 7298.0 29 8514.0 30 9961.0 31 12062.0 32 15130.0 33 19441.0 34 27483.0 35 41199.0 36 70466.0 37 137720.0 38 199184.0 39 69140.0 40 99.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09321061396953 20.217799407981076 13.418997569544569 23.269992408504827 2 15.229749770485629 21.41545632164968 40.708640333325484 22.646153574539206 3 17.60354724908715 27.955476963182875 30.794806234166693 23.646169553563283 4 11.961191043634331 18.53841174566746 40.4150116220908 29.08538558860741 5 12.678033128395704 38.44426888169964 35.868204360919734 13.00949362898492 6 28.12218169834444 39.25797657526971 19.494813221540394 13.125028504845456 7 24.913234162568063 33.2479038638935 24.80392171286577 17.034940260672677 8 24.477059469129664 35.9858705278985 21.693388689949007 17.84368131302283 9 25.442965155712756 16.16920290548066 21.556877417494384 36.8309545213122 10-11 23.569637833829642 27.010775532303054 30.07019397135156 19.349392662515744 12-13 24.582436514019808 25.077863287221003 29.62254879604306 20.71715140271613 14-15 22.107579052052102 26.30990875949993 27.222828661462735 24.359683526985236 16-17 21.5832624731148 29.073310167681264 27.761268245893294 21.582159113310638 18-19 21.291565463904636 28.129022671870608 29.57854535771713 21.000866506507627 20-21 22.436692426629712 27.18220429371948 29.327960667140378 21.053142612510428 22-23 22.110259643011087 27.29407230106761 29.026202627177117 21.569465428744184 24-25 21.745661151249536 27.53133224760972 29.239360864812696 21.483645736328047 26-27 21.69344629252429 27.78887312246539 29.08228381180628 21.435396773204044 28-29 21.667892661684224 27.602468663567354 29.21923733301948 21.510401341728947 30-31 21.871533203268683 27.45636801822082 29.173397953688745 21.49870082482175 32-33 21.70347736410593 27.653187473427298 28.991715938029156 21.651619224437617 34-35 21.976499907317777 27.703084405553874 28.975626046352883 21.34478964077547 36-37 21.980177773331647 27.635485228218943 28.871395323519806 21.512941674929607 38-39 21.734806540427503 27.768938633289274 28.954168606742414 21.54208621954081 40-41 21.95068128790041 27.586422495593915 28.968123199684587 21.49477301682109 42-43 21.80273207201866 27.637973609046394 28.847037950511993 21.71225636842295 44-45 22.005756667870568 27.78846415543739 28.586938851770583 21.61884032492146 46-47 21.906733998065388 27.800977604831385 28.487287091423337 21.80500130567989 48-49 21.88465700240025 27.97304186058829 28.40733801614046 21.734963120871004 50-51 21.947580971436558 28.078165113319553 28.25588665837888 21.718367256865008 52-53 22.07543450309088 28.006214122417035 28.17583730297672 21.74251407151537 54-55 21.94590006208238 28.009377087188962 28.294853047185548 21.74986980354311 56-57 21.99525702398851 28.26991711561151 28.004669418691208 21.73015644170877 58-59 21.996581055753506 28.104633816948194 28.18687090101833 21.711914226279973 60-61 22.07933304106558 28.088009862565507 28.28418723574533 21.54846986062359 62-63 21.948013449388622 28.060261852623125 28.3093300536166 21.682394644371655 64-65 21.97622259721268 27.882101626991073 28.317206618597922 21.82446915719832 66-67 21.8499689945632 28.08489384223933 28.26695423626952 21.798182926927943 68-69 22.0203699548683 28.05462884971181 28.18469053337796 21.740310662041924 70-71 21.990899434782225 27.893835566056335 28.27420270751378 21.841062291647663 72-73 21.971792238820022 27.987383448426023 28.152151845847396 21.888672466906563 74-75 21.917359821814745 28.164215246372887 28.023941436603888 21.894483495208476 76-77 21.90235412847816 28.057998475892326 28.08352286602858 21.956124529600938 78-79 21.89801424658179 28.148768209114632 27.965169137702244 21.98804840660133 80-81 21.90026160985701 28.213254703239077 27.866526003548948 22.019957683354964 82-83 21.999529176352883 28.168643145101964 27.96331989524174 21.868507783303418 84-85 21.801762033511743 28.152292666777022 27.997792859901782 22.048152439809453 86-87 21.842245625393137 28.307725130861165 27.974530352800965 21.87549889094474 88-89 21.848830374293467 28.155694076500737 27.971405470980613 22.02407007822518 90-91 21.907512664811758 28.089103185661802 28.062471400258076 21.940912749268364 92-93 21.974675982938805 28.08189550532537 27.982803444607757 21.960625067128067 94-95 21.918803006903637 27.94864984376138 28.079394381295806 22.053152768039176 96-97 21.880241529688025 28.03140651023735 28.0898173814274 21.998534578647224 98-99 21.787381931370668 27.95567924912803 28.088102978577517 22.168835840923787 100-101 22.081844171780947 27.879826318027522 27.965607908974405 22.072721601217125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 9.0 2 27.0 3 50.0 4 46.5 5 46.5 6 70.0 7 132.5 8 247.5 9 321.5 10 310.0 11 287.0 12 302.5 13 358.5 14 417.5 15 490.0 16 523.0 17 561.5 18 658.0 19 716.5 20 832.5 21 1047.0 22 1301.0 23 1686.0 24 2245.5 25 2951.0 26 4014.5 27 5179.0 28 6487.5 29 8275.5 30 10193.5 31 12245.0 32 14329.5 33 16073.5 34 17841.0 35 19459.0 36 21315.0 37 22683.5 38 23139.0 39 23705.5 40 24007.0 41 24308.0 42 24605.5 43 24892.5 44 25201.5 45 25284.5 46 25262.0 47 25124.0 48 24720.5 49 24290.5 50 23635.0 51 22459.0 52 21290.5 53 20193.5 54 18961.0 55 17311.0 56 15439.0 57 13463.0 58 11679.5 59 10170.5 60 8501.5 61 6848.0 62 5358.5 63 4064.5 64 2981.5 65 2157.0 66 1623.0 67 1284.0 68 990.0 69 778.0 70 609.5 71 478.5 72 338.5 73 214.5 74 165.5 75 121.0 76 79.0 77 62.0 78 45.5 79 32.5 80 27.0 81 17.0 82 12.5 83 14.0 84 11.5 85 5.5 86 6.5 87 6.5 88 5.0 89 5.5 90 2.5 91 3.0 92 3.0 93 2.0 94 1.5 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005001897778863157 2 0.0067672734655207425 3 5.88458562219195E-4 4 2.942292811095975E-4 5 0.0033836367327603712 6 0.004854783138308358 7 0.005737470981637151 8 0.004119209935534364 9 0.008973993073842723 10-11 0.007944190589959132 12-13 0.0036778660138699687 14-15 0.0 16-17 0.0 18-19 1.4711464055479874E-4 20-21 7.355732027739937E-4 22-23 1.4711464055479874E-4 24-25 0.001618261046102786 26-27 0.0040456526152569654 28-29 0.003236522092205572 30-31 0.011769171244383899 32-33 4.413439216643962E-4 34-35 0.0 36-37 0.0 38-39 7.355732027739937E-5 40-41 0.0 42-43 2.206719608321981E-4 44-45 0.0012504744447157893 46-47 0.0028687354908185753 48-49 0.0031629647719281723 50-51 0.003972095294979566 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0012504744447157893 64-65 0.003089407451650774 66-67 0.0033100794124829714 68-69 0.00948889431578452 70-71 5.88458562219195E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015299922617699068 82-83 0.012063400525493496 84-85 0.019051345951846436 86-87 0.01610905314075046 88-89 0.014932136016312072 90-91 0.015152807977144269 92-93 0.01044513947939071 94-95 0.025377275495702782 96-97 0.010886483401055106 98-99 0.015520594578531267 100-101 0.01603549582047306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 679742.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.59544398619165 #Duplication Level Percentage of deduplicated Percentage of total 1 82.18713808483487 62.12973193477534 2 12.57076112259817 19.005845366143316 3 3.0017084132314924 6.807464406459646 4 0.99111659150954 2.996955951089785 5 0.4258153419157356 1.609484991412602 6 0.2270319536548509 1.0297548801354566 7 0.1459532820736337 0.7723382211718745 8 0.09950616982281427 0.6017770469696825 9 0.06253487626366085 0.4254616562195699 >10 0.27652172829556526 3.4400386479423513 >50 0.0101549099588936 0.523780461296618 >100 0.0011716838949871206 0.14348982194875787 >500 1.9528064859212092E-4 0.10318866805230766 >1k 3.9056129718424183E-4 0.41068794638271805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.21552294841278014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.19374998161066992 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.10283313374780431 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.942292811095975E-4 0.0 20-21 0.0 0.0 0.0 2.942292811095975E-4 0.0 22-23 0.0 0.0 0.0 4.4134392166439624E-4 0.0 24-25 0.0 0.0 0.0 5.88458562219195E-4 7.355732027739937E-5 26-27 0.0 0.0 0.0 8.09130523051393E-4 1.4711464055479874E-4 28-29 0.0 0.0 0.0 0.0012504744447157893 1.4711464055479874E-4 30-31 0.0 0.0 0.0 0.0020596049677671824 1.4711464055479874E-4 32-33 0.0 0.0 0.0 0.004560553857198761 1.4711464055479874E-4 34-35 0.0 0.0 0.0 0.007649961308849535 1.4711464055479874E-4 36-37 0.0 0.0 0.0 0.012946088368822288 1.4711464055479874E-4 38-39 0.0 0.0 0.0 0.022361425364329408 1.4711464055479874E-4 40-41 0.0 0.0 0.0 0.03633731621703529 1.4711464055479874E-4 42-43 0.0 0.0 0.0 0.049945420468354165 1.4711464055479874E-4 44-45 0.0 0.0 0.0 0.06745206269437523 1.4711464055479874E-4 46-47 0.0 0.0 0.0 0.08657696596649905 1.4711464055479874E-4 48-49 0.0 7.355732027739937E-5 0.0 0.10783503152666747 1.4711464055479874E-4 50-51 0.0 1.4711464055479874E-4 0.0 0.13284452042098327 1.4711464055479874E-4 52-53 0.0 1.4711464055479874E-4 0.0 0.15851602519779562 1.4711464055479874E-4 54-55 0.0 1.4711464055479874E-4 0.0 0.1866149215437622 1.4711464055479874E-4 56-57 0.0 1.4711464055479874E-4 0.0 0.2191272571063727 1.4711464055479874E-4 58-59 0.0 1.4711464055479874E-4 0.0 0.26223184678892875 1.4711464055479874E-4 60-61 0.0 1.4711464055479874E-4 0.0 0.30518932183092995 1.4711464055479874E-4 62-63 0.0 1.4711464055479874E-4 0.0 0.348661698114873 1.4711464055479874E-4 64-65 0.0 1.4711464055479874E-4 0.0 0.394708580608525 1.4711464055479874E-4 66-67 0.0 2.942292811095975E-4 0.0 0.44884676833269094 1.4711464055479874E-4 68-69 0.0 2.942292811095975E-4 0.0 0.5107084746859838 1.4711464055479874E-4 70-71 0.0 2.942292811095975E-4 0.0 0.5801465850278489 1.4711464055479874E-4 72-73 0.0 2.942292811095975E-4 0.0 0.6568668700771764 1.4711464055479874E-4 74-75 0.0 2.942292811095975E-4 0.0 0.7398395273500828 1.4711464055479874E-4 76-77 0.0 2.942292811095975E-4 0.0 0.8291381141668457 1.4711464055479874E-4 78-79 0.0 2.942292811095975E-4 0.0 0.9250568598085744 1.4711464055479874E-4 80-81 0.0 2.942292811095975E-4 0.0 1.0359077414666151 1.4711464055479874E-4 82-83 0.0 2.942292811095975E-4 0.0 1.1566888613621051 1.4711464055479874E-4 84-85 0.0 2.942292811095975E-4 0.0 1.2786468983820332 1.4711464055479874E-4 86-87 0.0 2.942292811095975E-4 0.0 1.4218630009621296 1.4711464055479874E-4 88-89 0.0 2.942292811095975E-4 0.0 1.5722877209294115 1.4711464055479874E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1000 0.0 59.85019 1 GTATCAA 1750 0.0 38.814407 1 TCAACGC 2245 0.0 30.044636 4 ATCAACG 2250 0.0 29.97787 3 CAACGCA 2290 0.0 29.661663 5 TATCAAC 2395 0.0 28.162928 2 AACGCAG 2495 0.0 27.605295 6 ACATGGG 2275 0.0 26.93415 3 GTACATG 2415 0.0 26.749563 1 TACATGG 2365 0.0 26.511711 2 ACGCAGA 2700 0.0 25.157486 7 CGCAGAG 2745 0.0 24.572023 8 CATGGGG 1470 0.0 23.58851 4 GCAGAGT 2970 0.0 22.070774 9 AGAGTAC 2665 0.0 21.74411 10-11 ATAATAC 445 0.0 21.348381 3 CAGAGTA 2900 0.0 20.391554 10-11 TACCTGG 420 0.0 20.357208 2 GCATCGC 60 0.004765882 19.790272 36-37 TGGTATC 485 0.0 19.587688 2 >>END_MODULE