FastQCFastQC Report
Fri 10 Feb 2017
SRR2568657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679742
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.2651005822797473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.23950263482321235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.12563590303379812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.056.4419481
GTATCAA19900.040.143061
TTCGCTA250.0016156838.0011560-61
TCAACGC24250.032.1317754
ATCAACG24350.031.9998153
CACCGTA753.0726087E-431.6746147
CAACGCA24900.031.2929945
TATCAAC25550.030.8915352
AACGCAG25700.030.3188926
ACACCGT1004.941709E-528.5071536
GTGGTAT5200.028.3473591
ACGCAGA28050.027.6094237
CGCAGAG28450.027.2132328
GGCGTAG700.00834590227.1716631
TGGTATC5200.026.5165462
CTGGTCG1252.2552488E-422.7990119
GCAGAGT32950.022.7759489
GTACATG19550.022.1334381
CAGAGTA31250.022.03904310-11
CGCCGCG550.002877743721.59076764-65
Produced by FastQC (version 0.11.5)