##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568657_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679742 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64131685257053 34.0 31.0 34.0 31.0 34.0 2 32.752244233841665 34.0 31.0 34.0 31.0 34.0 3 32.804040356488194 34.0 31.0 34.0 31.0 34.0 4 36.218293117094426 37.0 37.0 37.0 35.0 37.0 5 36.26992741363635 37.0 37.0 37.0 35.0 37.0 6 36.28488308799515 37.0 37.0 37.0 35.0 37.0 7 36.22306698718043 37.0 37.0 37.0 35.0 37.0 8 36.22163556172783 37.0 37.0 37.0 35.0 37.0 9 38.04574971091973 39.0 39.0 39.0 35.0 39.0 10-11 37.976240985550405 39.0 38.5 39.0 35.0 39.0 12-13 37.96477339931916 39.0 38.5 39.0 35.0 39.0 14-15 39.40355458394508 41.0 39.0 41.0 36.0 41.0 16-17 39.380255302747216 41.0 39.0 41.0 36.0 41.0 18-19 39.44342927169426 41.0 39.0 41.0 36.0 41.0 20-21 39.42297665879113 41.0 39.0 41.0 36.0 41.0 22-23 39.382169999794044 41.0 39.0 41.0 36.0 41.0 24-25 39.35940033130217 41.0 39.0 41.0 36.0 41.0 26-27 39.250624501649156 41.0 39.0 41.0 36.0 41.0 28-29 39.14272253296103 41.0 39.0 41.0 36.0 41.0 30-31 39.065776426938456 40.5 39.0 41.0 35.5 41.0 32-33 38.906833033709844 40.0 38.0 41.0 35.0 41.0 34-35 38.79266324576089 40.0 38.0 41.0 35.0 41.0 36-37 38.690095653939295 40.0 38.0 41.0 35.0 41.0 38-39 38.546812614197734 40.0 38.0 41.0 34.5 41.0 40-41 38.45336686566374 40.0 38.0 41.0 34.0 41.0 42-43 38.312333944349476 40.0 38.0 41.0 34.0 41.0 44-45 38.17324220071733 40.0 38.0 41.0 33.5 41.0 46-47 37.94332629144587 40.0 38.0 41.0 33.0 41.0 48-49 37.75916891997258 40.0 38.0 41.0 33.0 41.0 50-51 37.7489650485037 40.0 37.5 41.0 33.0 41.0 52-53 37.81891438222149 40.0 38.0 41.0 33.0 41.0 54-55 37.68830747548334 40.0 37.0 41.0 33.0 41.0 56-57 37.55340261452139 40.0 37.0 41.0 32.5 41.0 58-59 37.35432561177623 40.0 36.5 41.0 32.0 41.0 60-61 37.2811566741499 40.0 36.0 41.0 32.0 41.0 62-63 37.10728482277099 39.0 36.0 41.0 32.0 41.0 64-65 36.88161317087954 39.0 35.5 41.0 31.0 41.0 66-67 36.64573249850679 39.0 35.0 41.0 31.0 41.0 68-69 36.349885691924285 38.0 35.0 40.5 31.0 41.0 70-71 35.98660447640428 37.0 35.0 40.0 31.0 41.0 72-73 35.596744794348446 37.0 35.0 39.0 30.0 41.0 74-75 35.229428959811216 36.0 35.0 39.0 30.0 41.0 76-77 33.43529824551079 34.5 32.0 36.5 27.5 39.0 78-79 34.39286817645518 35.0 34.0 37.0 29.5 39.0 80-81 34.28971653951058 35.0 34.0 37.0 30.0 39.0 82-83 34.055813087906884 35.0 34.0 36.5 30.0 39.0 84-85 33.7502118450824 35.0 34.0 36.0 29.0 37.0 86-87 33.51854306486873 35.0 34.0 36.0 29.0 37.0 88-89 33.326084749802135 35.0 34.0 35.5 29.0 37.0 90-91 33.18207790602905 35.0 34.0 35.0 29.0 36.0 92-93 33.06229495896973 35.0 34.0 35.0 29.0 36.0 94-95 32.952875502764286 35.0 34.0 35.0 29.0 36.0 96-97 32.853081757490344 35.0 34.0 35.0 29.0 36.0 98-99 32.765984741269484 35.0 34.0 35.0 29.0 35.5 100-101 31.67356291063374 34.5 32.0 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2635079005762293 1101 2 0.16454166975064055 1101 3 0.10511378457830745 1101 4 0.11213449482100657 1101 5 0.17050591199420495 1101 6 0.1447740273895448 1101 7 0.2399290795319473 1101 8 0.21254495385136352 1101 9 0.2484980725659227 1101 10-11 0.3253079476946752 1101 12-13 0.23723420434503595 1101 14-15 0.22377534486562922 1101 16-17 0.36150079760395215 1101 18-19 0.4261998567385916 1101 20-21 0.3739579940844564 1101 22-23 0.26275712474219404 1101 24-25 0.3627788010923254 1101 26-27 0.14768324348642636 1101 28-29 0.1831979823076466 1101 30-31 0.23968161317650782 1101 32-33 0.09717368983098851 1101 34-35 0.2150458488698419 1101 36-37 -0.04234741993352742 1101 38-39 0.22786378713572475 1101 40-41 0.25509844204513854 1101 42-43 0.23848010247078832 1101 44-45 0.19410523749625952 1101 46-47 0.3395412342931152 1101 48-49 0.2656875678262054 1101 50-51 0.20588508366920166 1101 52-53 0.24607831146550296 1101 54-55 0.26249772370669433 1101 56-57 0.29243695909972445 1101 58-59 0.27633975356246765 1101 60-61 0.4122673182260854 1101 62-63 0.4213942160332209 1101 64-65 0.3214708604150829 1101 66-67 0.03924291391465573 1101 68-69 0.2196139703865967 1101 70-71 0.2859000633592146 1101 72-73 0.2462849633430224 1101 74-75 0.3047372306332079 1101 76-77 0.23966315096418356 1101 78-79 0.4936247395309792 1101 80-81 0.5310737201971776 1101 82-83 0.5121158549559368 1101 84-85 0.3739736968993128 1101 86-87 0.36557813399528527 1101 88-89 0.30216947769062585 1101 90-91 0.30350167549362794 1101 92-93 0.3660242122197488 1101 94-95 0.32838629892553683 1101 96-97 0.14109623562854523 1101 98-99 0.1298566916024697 1101 100-101 0.10720673579467288 1102 1 0.30334052441070725 1102 2 0.10649623372496109 1102 3 0.22141539886382589 1102 4 0.18956353322761288 1102 5 0.19578795535090876 1102 6 0.1899407713283665 1102 7 0.07097937199533533 1102 8 0.13080740754661235 1102 9 0.15930961749382533 1102 10-11 0.20013291456131554 1102 12-13 0.1412844359273393 1102 14-15 0.1837390035381361 1102 16-17 0.2601536864116696 1102 18-19 0.3743467300263177 1102 20-21 0.3076854153956461 1102 22-23 0.31521437919236917 1102 24-25 0.4128207153811161 1102 26-27 0.25631969439987756 1102 28-29 0.3232771745560825 1102 30-31 0.3438573534671434 1102 32-33 0.324931017806783 1102 34-35 0.5544017269390693 1102 36-37 0.5391559683332829 1102 38-39 0.6341127626943148 1102 40-41 0.4932313126821839 1102 42-43 0.7031433129648192 1102 44-45 0.8524944175216476 1102 46-47 0.8565914518259277 1102 48-49 0.7602082697383423 1102 50-51 0.7418119514307975 1102 52-53 0.8682233395878853 1102 54-55 0.716613285381591 1102 56-57 0.6175068552740512 1102 58-59 0.5451403024289121 1102 60-61 0.6679585293382857 1102 62-63 0.8509185005633313 1102 64-65 0.8677170729088175 1102 66-67 0.8821369199372029 1102 68-69 0.832537854974781 1102 70-71 0.7953061917433786 1102 72-73 0.7892435969840434 1102 74-75 0.5240051589433818 1102 76-77 0.3708103848552824 1102 78-79 0.6068729330017177 1102 80-81 0.6352377525859154 1102 82-83 0.5111733688532141 1102 84-85 0.752912820492611 1102 86-87 0.5315938413164716 1102 88-89 0.7032599516733242 1102 90-91 0.6008648218283525 1102 92-93 0.463922175513126 1102 94-95 0.536599626264433 1102 96-97 0.5446875174564738 1102 98-99 0.6176863275803868 1102 100-101 0.5294557300858997 1103 1 0.32463390345367316 1103 2 0.2315708357319295 1103 3 0.28739827163096976 1103 4 0.17281335857795455 1103 5 0.22236014927449332 1103 6 0.07111654522297783 1103 7 0.22337103137529368 1103 8 0.19794458733629483 1103 9 0.2233331755353376 1103 10-11 0.34207682310503884 1103 12-13 0.2531053263398846 1103 14-15 0.3879657566274659 1103 16-17 0.23026314895336242 1103 18-19 0.27219749825349027 1103 20-21 0.32291694763991075 1103 22-23 0.3398469209348036 1103 24-25 0.33451752357184006 1103 26-27 0.36611881259258894 1103 28-29 0.232206051662871 1103 30-31 0.23067627762524268 1103 32-33 0.39161595907361146 1103 34-35 0.31869177028919893 1103 36-37 0.343319338632206 1103 38-39 0.4956317752181931 1103 40-41 0.39868570251873336 1103 42-43 0.4565102345505849 1103 44-45 0.4016577184233512 1103 46-47 0.18629878063456573 1103 48-49 0.38570619476158896 1103 50-51 0.392676872063511 1103 52-53 0.45873003179272587 1103 54-55 0.2896272527278825 1103 56-57 0.3473018558986283 1103 58-59 0.6830938710650756 1103 60-61 0.4588277063335653 1103 62-63 0.36280277376629755 1103 64-65 0.3206541944048311 1103 66-67 0.38345084055603706 1103 68-69 0.29725052736903024 1103 70-71 0.32933858636371127 1103 72-73 0.3170256157027822 1103 74-75 0.3375811049430979 1103 76-77 0.15065003102201047 1103 78-79 -0.02828971093967425 1103 80-81 7.956830684179295E-4 1103 82-83 0.1113123946088379 1103 84-85 0.2826113589872463 1103 86-87 0.11685526062832707 1103 88-89 0.19249528688501272 1103 90-91 0.2540371998858788 1103 92-93 0.30354636645726174 1103 94-95 0.2018690956533149 1103 96-97 0.21082329542239364 1103 98-99 0.3134038253943743 1103 100-101 0.40706564370950105 1104 1 0.18277545174691312 1104 2 0.13203004877732383 1104 3 0.11917188718619798 1104 4 0.0471857897294683 1104 5 0.0161717068055367 1104 6 -0.006440923348371541 1104 7 0.04770530634081638 1104 8 0.15589468529282158 1104 9 0.1508118772432212 1104 10-11 0.18723745841669626 1104 12-13 0.15053993645506836 1104 14-15 0.1203915975139438 1104 16-17 0.20659627253924384 1104 18-19 0.20009569487164924 1104 20-21 0.2957033310136268 1104 22-23 0.1177366384005154 1104 24-25 0.2317836663161401 1104 26-27 0.23427022347893 1104 28-29 0.08702511101247268 1104 30-31 0.048344376822399227 1104 32-33 0.09471397361311773 1104 34-35 0.1810762418371823 1104 36-37 0.22620773959793183 1104 38-39 0.1477321505322564 1104 40-41 0.17467430475277013 1104 42-43 0.13706932308632958 1104 44-45 0.08372631922931362 1104 46-47 0.25882007892668213 1104 48-49 0.03104667735924238 1104 50-51 0.25146662924736773 1104 52-53 0.3332039896379513 1104 54-55 0.2503877434476678 1104 56-57 0.2561431574038906 1104 58-59 0.08443558537118889 1104 60-61 0.2722249903271603 1104 62-63 0.25652998604410726 1104 64-65 0.22136108795577059 1104 66-67 0.23956297065301158 1104 68-69 0.23186398958548438 1104 70-71 0.21700655167951766 1104 72-73 0.3493434589634177 1104 74-75 0.37262901215439825 1104 76-77 0.3056768296064405 1104 78-79 0.3300671631984642 1104 80-81 0.3586281885687086 1104 82-83 0.33525796737558977 1104 84-85 0.40886259181138485 1104 86-87 0.2680218134958423 1104 88-89 0.18171114379261866 1104 90-91 0.19640350819678787 1104 92-93 0.22462553942750674 1104 94-95 0.25432715731906796 1104 96-97 0.33831962484192246 1104 98-99 0.25377195449170387 1104 100-101 0.10760652438104756 1105 1 0.2835354137488082 1105 2 0.2062163394627916 1105 3 0.2070474934058879 1105 4 0.16428493477221195 1105 5 0.1473351486334451 1105 6 0.1753499871061095 1105 7 0.15529492685979562 1105 8 0.022291365458812606 1105 9 0.15145184524482147 1105 10-11 0.22863288864517983 1105 12-13 0.19456023544011458 1105 14-15 0.31288947262018496 1105 16-17 0.3474392303913518 1105 18-19 0.22866176656806658 1105 20-21 0.3266067858408874 1105 22-23 0.35115746735906583 1105 24-25 0.32493650867402124 1105 26-27 0.31965095444238045 1105 28-29 0.30261683142644813 1105 30-31 0.4189208479988409 1105 32-33 0.4826695758330075 1105 34-35 0.3436206146185441 1105 36-37 0.3320549472375447 1105 38-39 0.4596090566869506 1105 40-41 0.4448432963031905 1105 42-43 0.4341519689540334 1105 44-45 0.417917109689796 1105 46-47 0.5395360431284715 1105 48-49 0.4805317031079568 1105 50-51 0.29951422686747975 1105 52-53 0.46790975434971926 1105 54-55 0.44983777094629573 1105 56-57 0.369504989312297 1105 58-59 0.4710512545877279 1105 60-61 0.4097431144209551 1105 62-63 0.4768864682199947 1105 64-65 0.49615484826775713 1105 66-67 0.40209234418433937 1105 68-69 0.43030906733159213 1105 70-71 0.3781859927074649 1105 72-73 0.2522483137206777 1105 74-75 0.3017250573521366 1105 76-77 0.33368292930145316 1105 78-79 0.2757398795626429 1105 80-81 0.250961071924543 1105 82-83 0.28556045225133886 1105 84-85 0.2849062896264911 1105 86-87 0.3610371627283868 1105 88-89 0.4805936996648299 1105 90-91 0.36120276823378816 1105 92-93 0.4794277993145144 1105 94-95 0.47278373449020705 1105 96-97 0.37115048329900446 1105 98-99 0.23738777370074615 1105 100-101 0.3241506971724064 1106 1 0.1943358591280031 1106 2 0.002826508954314022 1106 3 0.2466614981071089 1106 4 0.12298838646093913 1106 5 0.1356192015840847 1106 6 0.056087337099917534 1106 7 0.2081342192017459 1106 8 0.12845244426618052 1106 9 0.0301973605238004 1106 10-11 0.1834640157019649 1106 12-13 0.03864040369447963 1106 14-15 0.06409570792885688 1106 16-17 0.21917620923584025 1106 18-19 0.19600681172456547 1106 20-21 0.13242145861089227 1106 22-23 0.12600591471772304 1106 24-25 0.23398636640011006 1106 26-27 0.10906082701444575 1106 28-29 0.10301890986267637 1106 30-31 0.1618566391654852 1106 32-33 0.1643347991411872 1106 34-35 0.1742280623483481 1106 36-37 0.17254733867637384 1106 38-39 0.2497302988000314 1106 40-41 0.21808342991694474 1106 42-43 0.19318326921384 1106 44-45 0.26530050694329077 1106 46-47 0.20644189491617482 1106 48-49 0.3763872023038033 1106 50-51 0.40278652675613813 1106 52-53 0.3493646597066373 1106 54-55 0.3321772488629122 1106 56-57 0.315156356378985 1106 58-59 0.23102279980608387 1106 60-61 0.4832244950964508 1106 62-63 0.44633469288905303 1106 64-65 0.4920412765740778 1106 66-67 0.5116308818559929 1106 68-69 0.4046812063888794 1106 70-71 0.42509926748168425 1106 72-73 0.2696985178528095 1106 74-75 0.4417287688453868 1106 76-77 0.2588592252311841 1106 78-79 0.43604545917417425 1106 80-81 0.44194214330892834 1106 82-83 0.44098541822932447 1106 84-85 0.5344670962576927 1106 86-87 0.24787991913070329 1106 88-89 0.3233686594569818 1106 90-91 0.3622110572353918 1106 92-93 0.3489564396708147 1106 94-95 0.16267610118574538 1106 96-97 0.45256827372335096 1106 98-99 0.33905175984450153 1106 100-101 0.2916256445929193 1107 1 0.08240547024598754 1107 2 0.1364423281633549 1107 3 -0.001709568741006251 1107 4 0.13349397432023835 1107 5 0.06513175880294142 1107 6 -0.08736187730067257 1107 7 0.07591639578634357 1107 8 0.11353430331191561 1107 9 -0.03414137509416548 1107 10-11 0.027926673955917636 1107 12-13 0.18382577216328144 1107 14-15 0.04424540482357742 1107 16-17 0.057891207792479804 1107 18-19 0.03614764227427969 1107 20-21 0.04016610787478214 1107 22-23 -0.015650442245458862 1107 24-25 0.005444983250292523 1107 26-27 0.01442496574181007 1107 28-29 -0.12097073919501611 1107 30-31 0.013977345173977085 1107 32-33 0.12744358713244708 1107 34-35 -0.09338503509896867 1107 36-37 0.025699015034156503 1107 38-39 0.1428011470824373 1107 40-41 0.1691370816139255 1107 42-43 0.04064914409527631 1107 44-45 -0.10002074341754508 1107 46-47 -0.03221536930090707 1107 48-49 -0.05718016129882386 1107 50-51 0.07846902912736908 1107 52-53 0.15717529107288186 1107 54-55 0.11353833591804374 1107 56-57 0.10636939609196361 1107 58-59 0.046474983401289194 1107 60-61 -0.06412694207621428 1107 62-63 0.10287516878496916 1107 64-65 0.14417744713781389 1107 66-67 -0.0498850569456053 1107 68-69 -0.028447994815302025 1107 70-71 0.1055871226509737 1107 72-73 0.16750255100881617 1107 74-75 0.05906969012049501 1107 76-77 -0.29513063002058004 1107 78-79 -0.1030736797593832 1107 80-81 0.12963338830872573 1107 82-83 0.04897853134738739 1107 84-85 -0.018059812068422332 1107 86-87 0.02742134351934311 1107 88-89 -0.11206166756681313 1107 90-91 -0.06280853120124164 1107 92-93 -0.05503547752164906 1107 94-95 0.09029785878399821 1107 96-97 0.11804313866623772 1107 98-99 0.10687929912447203 1107 100-101 0.1443484372854016 1108 1 0.048569335367723454 1108 2 -0.0612725281846096 1108 3 -0.041333361279029646 1108 4 0.14824968426513863 1108 5 0.07910256989099906 1108 6 0.057964865990776104 1108 7 0.079695178649132 1108 8 0.03817041332209925 1108 9 0.0416693632547549 1108 10-11 0.09357355495436792 1108 12-13 0.030102165358748323 1108 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72-73 -0.21328211726069668 2316 74-75 -0.0695358960128587 2316 76-77 -0.09614201555915969 2316 78-79 -0.1296300014157552 2316 80-81 -0.2917217822094216 2316 82-83 -0.27543066583636744 2316 84-85 -0.22363783181924646 2316 86-87 -0.35616334594489985 2316 88-89 -0.35124904876047225 2316 90-91 -0.24796427927550013 2316 92-93 -0.23649208464440363 2316 94-95 -0.1700858981125748 2316 96-97 -0.19215152158902526 2316 98-99 -0.29849455586390405 2316 100-101 -0.3515641278718036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 16.0 15 67.0 16 205.0 17 463.0 18 823.0 19 1260.0 20 1951.0 21 2825.0 22 3879.0 23 4038.0 24 3859.0 25 3705.0 26 3833.0 27 4564.0 28 5616.0 29 6998.0 30 8649.0 31 10838.0 32 13789.0 33 17944.0 34 25121.0 35 37623.0 36 67625.0 37 144480.0 38 229154.0 39 80315.0 40 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80961846594796 20.292620771543042 13.848283898647985 23.04947686386101 2 15.341845291648198 21.47431319510604 40.67554772846745 22.50829378477831 3 17.490900959481685 27.8012834281242 31.022505597712076 23.68531001468204 4 12.00190660574159 18.621182742864203 40.240267630954094 29.136643020440108 5 12.692462728505816 38.32601192805505 36.046029228736785 12.935496114702344 6 28.096739407082 39.394429789782016 19.330521403571748 13.178309399564233 7 24.708328673263363 33.42315005032875 24.74570733975736 17.12281393665052 8 24.126801050986995 36.278176131532256 21.621585836979325 17.973436980501425 9 25.236339670051283 16.15745385749299 21.58951484533838 37.016691627117346 10-11 23.38754998219913 27.0546766273086 30.16563637380065 19.392137016691628 12-13 24.43625669739401 24.992791382612815 29.841175034057038 20.729776885936136 14-15 22.00378967314069 26.319691883096823 27.450856354322667 24.22566208943982 16-17 21.26586263611782 29.177835119795454 27.96274174613309 21.593560497953636 18-19 20.975826122264035 28.118903937082013 29.914511682373607 20.990758258280348 20-21 22.0493216543924 27.113154696929133 29.86772922667718 20.96979442200129 22-23 21.69124461928202 27.200540793418682 29.569674964913155 21.53853962238614 24-25 21.43754542164527 27.43607133294691 29.69766470219583 21.428718543211982 26-27 21.40363549699739 27.629600642596753 29.602775759037986 21.363988101367873 28-29 21.367507536047242 27.466887733860208 29.7448409890354 21.42076374105715 30-31 21.648802045481965 27.34486025580294 29.62675544544842 21.37958225326668 32-33 21.3632525281651 27.447766946871017 29.616163191328475 21.572817333635406 34-35 21.654171729862213 27.59907435468163 29.434329495602746 21.312424419853414 36-37 21.689479243595365 27.589732575006398 29.36113996192673 21.359648219471506 38-39 21.48675526891085 27.76671148759382 29.392769609646013 21.35376363384931 40-41 21.662263035092728 27.523089642835075 29.386590794742713 21.42805652732949 42-43 21.562739980757406 27.600692615727734 29.175628400187133 21.660939003327734 44-45 21.643064574500325 27.739789508372294 29.168125553518838 21.449020363608547 46-47 21.650420306528066 27.771124926810465 28.86764390018566 21.71081086647581 48-49 21.517428671466526 27.957151389792013 28.86536362325706 21.660056315484404 50-51 21.737291501775672 27.91036893409558 28.654474786021755 21.697864778106986 52-53 21.755607274524746 27.988486808230185 28.550170505868405 21.70573541137667 54-55 21.663734181498274 28.005699221175096 28.611223081698643 21.71934351562799 56-57 21.69661430366227 28.162964771928173 28.496915005987567 21.64350591842199 58-59 21.831959772972688 28.11831547851979 28.454325317546953 21.595399430960573 60-61 21.83019439728603 28.0909521553766 28.60467648019396 21.474176967143414 62-63 21.715729543649044 28.018948421455264 28.680966251802158 21.584355783093535 64-65 21.744033200905346 27.89218826890556 28.612316023541336 21.751462506647755 66-67 21.576348085008725 28.122728917736435 28.44410085002839 21.85682214722645 68-69 21.739866007985384 28.02416210856472 28.568265606656645 21.667706276793254 70-71 21.695658058498665 27.872486914152724 28.49654721938618 21.935307807962435 72-73 21.656378449470534 28.064177290795627 28.337295620985607 21.942148638748233 74-75 21.685139361698997 28.10110306557488 28.2312259651456 21.982531607580526 76-77 21.71949063026854 27.996578113460696 28.383489618119818 21.900441638150944 78-79 21.659762086203294 28.09058436877521 28.3425181907253 21.90713535429619 80-81 21.737806403017615 28.157153743626257 28.139720658720513 21.96531919463561 82-83 21.780469648778507 28.08756851864384 28.209820784944878 21.922141047632778 84-85 21.73942466406372 28.049760056021256 28.215190469325123 21.995624810589902 86-87 21.71169355431914 28.100882393614047 28.25491142227492 21.93251262979189 88-89 21.743249644718144 28.097572314201564 28.194962206248842 21.964215834831453 90-91 21.815777162511658 27.955018227503963 28.261752253060717 21.967452356923655 92-93 21.88190519344104 27.96399222057781 28.17443971389145 21.979662872089705 94-95 21.75546015988419 27.97576139182219 28.165097934216217 22.1036805140774 96-97 21.78745759420486 27.902866087427284 28.225120707562574 22.084555610805275 98-99 21.629871987913035 27.898219982478604 28.27306891379872 22.198839115809633 100-101 21.81223431761325 27.853628359854675 28.098503020660242 22.235634301871833 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12.0 1 11.0 2 32.5 3 62.0 4 49.5 5 41.0 6 68.5 7 152.0 8 276.5 9 376.5 10 379.0 11 336.0 12 375.0 13 408.0 14 447.5 15 519.0 16 553.0 17 600.0 18 679.0 19 777.5 20 872.0 21 1056.0 22 1369.0 23 1720.5 24 2281.5 25 3042.5 26 4017.0 27 5275.0 28 6579.5 29 8200.0 30 10261.0 31 12117.5 32 13968.0 33 16014.5 34 18160.5 35 19650.5 36 21106.5 37 22525.0 38 23349.5 39 24155.0 40 24320.0 41 24348.0 42 24800.0 43 25400.0 44 25671.0 45 25742.0 46 25549.5 47 25470.5 48 25458.0 49 24927.5 50 24224.5 51 23073.5 52 21910.5 53 20746.0 54 19520.5 55 17865.0 56 15723.0 57 13549.0 58 11479.5 59 9625.0 60 7683.5 61 5975.5 62 4444.0 63 3074.0 64 2070.5 65 1442.0 66 1008.0 67 747.0 68 569.0 69 428.0 70 327.0 71 221.0 72 147.5 73 115.5 74 81.5 75 51.0 76 34.0 77 21.5 78 13.0 79 7.0 80 3.5 81 1.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013387432290486685 2 0.09106396250342041 3 0.0 4 0.0 5 0.0 6 0.002795178170541176 7 0.030894074516507733 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0013240317649931886 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.942292811095975E-4 64-65 2.206719608321981E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.206719608321981E-4 100-101 9.562451636061917E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 679742.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.24113041279914 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21320271462272 60.29359973977398 2 13.167769334451076 19.55180160809279 3 3.1595676818629603 7.0370962895176055 4 1.05716665025405 3.1394098859829183 5 0.49560223862112124 1.839703521517293 6 0.2721936753113049 1.2124779687795415 7 0.15337400850789842 0.7970661837298056 8 0.10124103255009383 0.6012998960542366 9 0.06848458784113468 0.45759358954624296 >10 0.297670176699332 3.6485893966863845 >50 0.010942585925021864 0.5543427470656231 >100 0.0021884604940341813 0.23425765113641792 >500 1.9895095308221928E-4 0.12613873480112678 >1k 3.9790190616443855E-4 0.506622787316001 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.2651005822797473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1628 0.23950263482321235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.12563590303379812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.942292811095975E-4 2 0.0 0.0 0.0 0.0 2.942292811095975E-4 3 0.0 0.0 0.0 0.0 2.942292811095975E-4 4 0.0 0.0 0.0 0.0 2.942292811095975E-4 5 0.0 0.0 0.0 0.0 2.942292811095975E-4 6 0.0 0.0 0.0 0.0 2.942292811095975E-4 7 0.0 0.0 0.0 0.0 2.942292811095975E-4 8 0.0 0.0 0.0 0.0 2.942292811095975E-4 9 0.0 0.0 0.0 0.0 2.942292811095975E-4 10-11 0.0 0.0 0.0 0.0 2.942292811095975E-4 12-13 0.0 0.0 0.0 0.0 2.942292811095975E-4 14-15 0.0 0.0 0.0 0.0 2.942292811095975E-4 16-17 0.0 0.0 0.0 0.0 2.942292811095975E-4 18-19 0.0 0.0 0.0 0.0 2.942292811095975E-4 20-21 0.0 0.0 0.0 0.0 2.942292811095975E-4 22-23 0.0 0.0 0.0 1.4711464055479874E-4 2.942292811095975E-4 24-25 0.0 0.0 0.0 3.6778660138699683E-4 2.942292811095975E-4 26-27 0.0 0.0 0.0 6.620158824965943E-4 3.6778660138699683E-4 28-29 0.0 0.0 0.0 0.00117691712443839 4.413439216643962E-4 30-31 0.0 0.0 0.0 0.001986047647489783 4.413439216643962E-4 32-33 0.0 0.0 0.0 0.004413439216643962 4.413439216643962E-4 34-35 0.0 0.0 0.0 0.007355732027739937 4.413439216643962E-4 36-37 0.0 0.0 0.0 0.012578301767435292 4.413439216643962E-4 38-39 0.0 0.0 0.0 0.02199363876294241 4.413439216643962E-4 40-41 0.0 0.0 0.0 0.03596952961564829 4.413439216643962E-4 42-43 0.0 0.0 0.0 0.049945420468354165 4.413439216643962E-4 44-45 0.0 0.0 0.0 0.06723139073354302 4.413439216643962E-4 46-47 0.0 0.0 0.0 0.08620917936511205 4.413439216643962E-4 48-49 0.0 0.0 0.0 0.10783503152666747 4.413439216643962E-4 50-51 0.0 0.0 0.0 0.13313874970209286 4.413439216643962E-4 52-53 0.0 0.0 0.0 0.15873669715862782 4.413439216643962E-4 54-55 0.0 7.355732027739937E-5 0.0 0.18698270814514917 4.413439216643962E-4 56-57 0.0 1.4711464055479874E-4 0.0 0.2197892729888693 4.413439216643962E-4 58-59 0.0 1.4711464055479874E-4 0.0 0.26333520659308973 4.413439216643962E-4 60-61 0.0 1.4711464055479874E-4 0.0 0.30688114019731016 4.413439216643962E-4 62-63 0.0 1.4711464055479874E-4 0.0 0.35072130308264016 4.413439216643962E-4 64-65 0.0 1.4711464055479874E-4 0.0 0.3967681855762922 4.413439216643962E-4 66-67 0.0 1.4711464055479874E-4 0.0 0.45149483186267736 4.413439216643962E-4 68-69 0.0 1.4711464055479874E-4 0.0 0.5133565382159702 4.413439216643962E-4 70-71 0.0 1.4711464055479874E-4 0.0 0.5821326326753387 5.88458562219195E-4 72-73 0.0 1.4711464055479874E-4 0.0 0.6592942616463305 5.88458562219195E-4 74-75 0.0 1.4711464055479874E-4 0.0 0.7419726896381273 5.88458562219195E-4 76-77 0.0 1.4711464055479874E-4 0.0 0.8311977191346129 5.88458562219195E-4 78-79 0.0 1.4711464055479874E-4 0.0 0.9261602196127354 5.88458562219195E-4 80-81 0.0 1.4711464055479874E-4 0.0 1.0368639866302214 5.88458562219195E-4 82-83 0.0 1.4711464055479874E-4 0.0 1.1572037626040468 5.88458562219195E-4 84-85 0.0 1.4711464055479874E-4 0.0 1.2790882423036976 5.88458562219195E-4 86-87 0.0 1.4711464055479874E-4 0.0 1.423922605929897 5.88458562219195E-4 88-89 0.0 1.4711464055479874E-4 0.0 1.5758184723027266 5.88458562219195E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1230 0.0 56.441948 1 GTATCAA 1990 0.0 40.14306 1 TTCGCTA 25 0.00161568 38.00115 60-61 TCAACGC 2425 0.0 32.131775 4 ATCAACG 2435 0.0 31.999815 3 CACCGTA 75 3.0726087E-4 31.674614 7 CAACGCA 2490 0.0 31.292994 5 TATCAAC 2555 0.0 30.891535 2 AACGCAG 2570 0.0 30.318892 6 ACACCGT 100 4.941709E-5 28.507153 6 GTGGTAT 520 0.0 28.347359 1 ACGCAGA 2805 0.0 27.609423 7 CGCAGAG 2845 0.0 27.213232 8 GGCGTAG 70 0.008345902 27.171663 1 TGGTATC 520 0.0 26.516546 2 CTGGTCG 125 2.2552488E-4 22.799011 9 GCAGAGT 3295 0.0 22.775948 9 GTACATG 1955 0.0 22.133438 1 CAGAGTA 3125 0.0 22.039043 10-11 CGCCGCG 55 0.0028777437 21.590767 64-65 >>END_MODULE