FastQCFastQC Report
Fri 10 Feb 2017
SRR2568656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81335
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18382.25978975840659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12371.5208704739656975No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA12351.5184115079609024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9571.1766152332943998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9121.1212884981865125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5750.7069527263785579No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4820.5926108071555911No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4630.5692506301100387No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3640.447531812872687No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA3400.41802422081514723No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2910.3577795536976701No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.3504026556832852No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2780.3417962746665027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.3295014446425278No Hit
GTAATAAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGT2680.3295014446425278No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA2530.3110591996065654No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA2510.30860023360177047No Hit
GACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCACATTT2430.2987643695825905No Hit
AAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAA2420.297534886580193No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2390.29384643757300055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.29384643757300055No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2130.26187987951066577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.2581914305034733No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2080.2557324644986783No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2060.2532734984938833No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2020.24835556648429336No Hit
CCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCA1820.2237659064363435No Hit
CTTTTATCCAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAA1730.21270055941476607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1650.20286469539558616No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1620.19917624638839368No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.19548779738120123No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1580.19425831437880373No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1831929673572263No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1440.17704555234523883No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.17458658634044386No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.17458658634044386No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1390.17089813733325135No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1340.1647507223212639No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1340.1647507223212639No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1300.15983279031167394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.15737382430687896No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA1260.15491485830208396No Hit
GGTTAAGCATTCAACAATTATTCTGTTTTAAAAAGTGCTTTAACAACTAC1230.1512264092948915No Hit
GTACATGGGGATTAACACAGGTGACGATATCAAGGCCAGTTTATTGCAGA1230.1512264092948915No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1210.1487674432900965No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1170.14384951128050655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1120.1377020962685191No Hit
TTATTAAACTCTTTGTAGCACAAAGGATAAATACTTGAGGTGTTCAATAC1100.13524313026372412No Hit
GTGCTAAAGTTATCCTGGGAGAATTAGGGTCAAGTCTTATGATGCCAGAG1080.1327841642589291No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1070.13155468125653164No Hit
GTGTATATATTTATGGGATACATGAGATGGTTTGACACAGGCATGCAATG1060.13032519825413413No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1060.13032519825413413No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA1040.12786623224933916No Hit
GTACATGGGAAGATGCCAATTGCTGACTGGAAGTGCTGTGCTAAAGTTAT1020.12540726624454418No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT980.1204893342349542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG950.11680088522776173No Hit
GTACATGGGAGTGAGAAAAGATATTTCATAATGATAAAAGACACGATATG910.11188295321817175No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA910.11188295321817175No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA900.11065347021577426No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT890.10942398721337676No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT880.10819450421097929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT850.10450605520378681No Hit
TAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAA850.10450605520378681No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC830.10204708919899182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAGG301.3562767E-679.163083
AAGTACT408.958341E-871.246774
AGTACTC505.487891E-966.496995
AAAGTAC452.0244988E-763.3304633
ATAGGAG250.0050054556.9974175
CATAGGA504.1941348E-756.9974174
TAATAAA753.6379788E-1156.9974142
GTTAAGC352.7731428E-454.2832572
TTAAGCA352.7731428E-454.2832573
TAAATAA804.143658E-947.4978455
TAAGCAT405.3597067E-447.4978454
GTAATAA953.7471182E-1044.997961
ATTATTC652.5604531E-643.8441663
GGATTAA652.5604531E-643.8441669
GGGATTA652.5604531E-643.8441668
CATTCAA459.5744425E-442.220318
ATTCAAC459.5744425E-442.220319
GGTTAAG459.5744425E-442.220311
AAAAGTA704.2622014E-640.7124442
ATAATAC1051.0022632E-940.712443
Produced by FastQC (version 0.11.5)