##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568656_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81335 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.001598327903118 31.0 30.0 34.0 15.0 34.0 2 29.011053052191553 31.0 30.0 34.0 15.0 34.0 3 28.960177045552346 31.0 30.0 34.0 15.0 34.0 4 32.146566668715806 37.0 33.0 37.0 17.0 37.0 5 32.19299194688634 37.0 33.0 37.0 17.0 37.0 6 32.51576811950575 37.0 35.0 37.0 17.0 37.0 7 32.48808016229176 37.0 35.0 37.0 17.0 37.0 8 32.51442798303314 37.0 35.0 37.0 17.0 37.0 9 34.000799163951555 39.0 35.0 39.0 17.0 39.0 10-11 33.9112313272269 39.0 35.0 39.0 17.0 39.0 12-13 33.82250568635888 39.0 35.0 39.0 16.0 39.0 14-15 34.655394356673014 39.5 34.0 41.0 17.0 41.0 16-17 34.6641544230651 39.5 34.5 41.0 17.0 41.0 18-19 34.84928382615111 40.0 34.5 41.0 16.0 41.0 20-21 34.928444089260466 40.0 35.0 41.0 16.0 41.0 22-23 34.811010020286474 40.0 35.0 41.0 15.5 41.0 24-25 34.67349849388332 40.0 35.0 41.0 15.0 41.0 26-27 34.390219462715926 40.0 34.0 41.0 13.0 41.0 28-29 34.09740579086494 39.0 34.0 41.0 13.0 41.0 30-31 33.554779615171825 38.0 33.0 41.0 13.0 41.0 32-33 32.85107887133461 36.5 32.5 40.0 11.5 41.0 34-35 32.48187742054466 35.0 31.5 40.0 9.0 41.0 36-37 32.08734247249032 35.0 30.0 40.0 8.0 41.0 38-39 31.608305157681194 35.0 29.0 40.0 8.0 41.0 40-41 30.962525358086925 35.0 26.0 40.0 8.0 41.0 42-43 30.24617938157005 35.0 23.5 40.0 7.5 41.0 44-45 29.350212085817915 35.0 20.0 40.0 7.0 41.0 46-47 28.308753918977068 35.0 14.0 39.5 7.0 41.0 48-49 27.397424233109977 35.0 11.0 40.0 7.0 41.0 50-51 26.09032396877113 34.0 10.0 38.5 7.0 40.0 52-53 25.925265875699267 33.0 10.0 38.5 7.0 40.0 54-55 25.809639146738796 33.0 8.0 39.0 7.0 41.0 56-57 25.239226655191494 32.5 8.0 39.0 7.0 41.0 58-59 24.670449376037375 30.5 8.0 38.5 7.0 41.0 60-61 24.091110837892664 28.5 7.5 38.0 7.0 41.0 62-63 23.527343701973322 26.5 7.0 37.0 7.0 41.0 64-65 23.10336263601156 25.5 7.0 37.0 7.0 40.0 66-67 22.61005717095961 23.5 7.0 36.0 7.0 40.0 68-69 22.10098973381693 21.5 7.0 35.5 7.0 39.5 70-71 21.4742054466097 20.0 7.0 35.0 7.0 39.0 72-73 20.890797319727056 18.5 7.0 35.0 7.0 38.5 74-75 20.508883014692323 17.5 7.0 35.0 7.0 37.0 76-77 20.10514538636503 16.0 7.0 35.0 7.0 37.0 78-79 19.771205508083852 15.0 7.0 35.0 7.0 36.0 80-81 19.461959795905823 14.0 7.0 35.0 7.0 36.0 82-83 19.189174402163893 12.5 7.0 34.5 7.0 35.0 84-85 18.912989487920328 11.5 7.0 34.0 7.0 35.0 86-87 18.688946947808446 10.5 7.0 34.0 6.0 35.0 88-89 18.456181225794552 10.0 7.0 34.0 5.0 35.0 90-91 18.264916702526587 10.0 7.0 34.0 5.0 35.0 92-93 17.987324030245283 10.0 7.0 33.5 5.0 35.0 94-95 17.75678367246573 10.0 7.0 33.0 5.0 35.0 96-97 17.54621011864511 10.0 7.0 33.0 5.0 35.0 98-99 17.325302760189338 10.0 7.0 33.0 5.0 35.0 100-101 16.43706276510727 10.0 7.0 31.0 5.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7033269600302461 1101 2 0.9530375531780173 1101 3 1.1808311947431278 1101 4 1.2924264007771598 1101 5 0.7662836569220062 1101 6 0.6168606617986114 1101 7 0.6489264576064997 1101 8 1.2405301218287903 1101 9 0.7896839031229348 1101 10-11 0.7128730808265047 1101 12-13 0.6758601324113442 1101 14-15 0.6101245272449347 1101 16-17 1.1393312002291864 1101 18-19 0.7163513540992383 1101 20-21 1.5531265622210952 1101 22-23 0.7664436647667756 1101 24-25 1.0369626348645014 1101 26-27 1.3611110933678034 1101 28-29 1.2030731199490035 1101 30-31 1.6250346011013264 1101 32-33 2.0089700432471247 1101 34-35 1.885025286539232 1101 36-37 1.2549864926460828 1101 38-39 1.1633986685186777 1101 40-41 0.9399612098640695 1101 42-43 0.9679110351083153 1101 44-45 1.3589972901499436 1101 46-47 2.0223483007695364 1101 48-49 2.685796109073152 1101 50-51 3.242387321015915 1101 52-53 2.859530566212076 1101 54-55 2.3536015737556397 1101 56-57 3.2185596909378233 1101 58-59 3.731767006193955 1101 60-61 3.1350059248258653 1101 62-63 3.0073317999162086 1101 64-65 3.6949501891314895 1101 66-67 4.1447925626414595 1101 68-69 2.984986808807374 1101 70-71 3.16741472511875 1101 72-73 3.3550675721086023 1101 74-75 2.8819515820472184 1101 76-77 2.735749852439856 1101 78-79 3.034229995099512 1101 80-81 3.563041745909551 1101 82-83 3.4653330622849765 1101 84-85 2.7417048056875224 1101 86-87 3.75365251106178 1101 88-89 3.097744515253428 1101 90-91 2.428602356403971 1101 92-93 2.64431196523428 1101 94-95 2.0147530935720397 1101 96-97 1.9019602201817989 1101 98-99 1.722798968469455 1101 100-101 1.856408627704333 1102 1 1.5113323874386602 1102 2 1.4591433876420616 1102 3 1.59444494417777 1102 4 2.417482936281452 1102 5 1.7187938333127804 1102 6 1.4777833212287348 1102 7 2.151640161812736 1102 8 1.3456861598206515 1102 9 2.729756268568437 1102 10-11 2.4988296615592063 1102 12-13 2.2256113761924325 1102 14-15 2.744792098479671 1102 16-17 2.6838811776149853 1102 18-19 2.5624617114036212 1102 20-21 2.736188525133805 1102 22-23 2.224607391587213 1102 24-25 2.828685837035472 1102 26-27 2.3834991530692946 1102 28-29 3.0608297911385094 1102 30-31 2.1478749448371914 1102 32-33 1.887757922035 1102 34-35 1.2942292666387374 1102 36-37 1.486216705219583 1102 38-39 1.051797583036997 1102 40-41 1.8783375101806747 1102 42-43 1.9084356845881842 1102 44-45 1.285840347590014 1102 46-47 1.7021312044330372 1102 48-49 2.056103662216529 1102 50-51 1.6021792703601072 1102 52-53 1.6463917150135217 1102 54-55 2.075559963624478 1102 56-57 2.0032724905759984 1102 58-59 1.8835082997262909 1102 60-61 1.1927852102170888 1102 62-63 1.2093896922726088 1102 64-65 2.088663441053697 1102 66-67 1.5542226847581482 1102 68-69 2.567867858106336 1102 70-71 1.6663970860414068 1102 72-73 2.6588893721990594 1102 74-75 2.869626842110538 1102 76-77 2.3959714716167007 1102 78-79 2.568038226668939 1102 80-81 1.788618408053381 1102 82-83 1.6797156945780571 1102 84-85 1.6732968454885153 1102 86-87 1.547184849370236 1102 88-89 0.8883370073384533 1102 90-91 1.6502215332470271 1102 92-93 0.8748637517562159 1102 94-95 0.9588960153268999 1102 96-97 0.6509425811044594 1102 98-99 1.4032602981845166 1102 100-101 0.3697510067183565 1103 1 1.3471532829610453 1103 2 1.19048667122415 1103 3 0.7287733023867276 1103 4 1.1786769661322012 1103 5 1.4799878631635295 1103 6 1.2837534704824662 1103 7 0.9277595647978174 1103 8 0.9576264583281144 1103 9 1.8342338805087337 1103 10-11 1.2600236914099483 1103 12-13 1.650984510520793 1103 14-15 1.8567323969871339 1103 16-17 1.4600005806000595 1103 18-19 1.778879621851388 1103 20-21 1.0645467340890349 1103 22-23 1.7246073915872202 1103 24-25 1.7540589713638255 1103 26-27 1.547678257546913 1103 28-29 2.135456656810156 1103 30-31 1.7747406164789865 1103 32-33 2.1788026981544064 1103 34-35 1.8166173263402356 1103 36-37 1.1578584962643603 1103 38-39 1.3726931054250535 1103 40-41 1.2141584057030634 1103 42-43 1.3338088189165447 1103 44-45 0.5843478102765793 1103 46-47 -0.07398819855203698 1103 48-49 -0.16031424823123075 1103 50-51 -1.2336416251622815 1103 52-53 -0.8013694790163264 1103 54-55 -0.5960818274202992 1103 56-57 -0.8101603452448956 1103 58-59 -0.29559617788564907 1103 60-61 -0.16542374500679102 1103 62-63 0.08998670719797985 1103 64-65 -0.6799932753642146 1103 66-67 -1.5651803003164808 1103 68-69 -0.5216843806996359 1103 70-71 -1.2440506751526215 1103 72-73 -0.8858867472039265 1103 74-75 -1.2050000235611051 1103 76-77 -0.9323867373385255 1103 78-79 -1.140916997211658 1103 80-81 -1.457650248663036 1103 82-83 -1.3799857979592538 1103 84-85 -1.378941960481633 1103 86-87 -0.9005763446596085 1103 88-89 -0.8803197090794548 1103 90-91 -0.8124650339171531 1103 92-93 -1.0355840094378124 1103 94-95 -1.302298014523842 1103 96-97 -1.3565201054627032 1103 98-99 -1.7758441794274251 1103 100-101 -2.60039824701299 1104 1 1.8838082434527053 1104 2 1.4797755210924564 1104 3 1.8635406421584548 1104 4 2.244743691329738 1104 5 1.7021125339273553 1104 6 2.0927964906756173 1104 7 1.5807446394246796 1104 8 2.10951407904804 1104 9 2.1715912115008322 1104 10-11 1.7249051663880053 1104 12-13 2.104781701038789 1104 14-15 1.978440035266324 1104 16-17 1.558003214489439 1104 18-19 2.288098234669647 1104 20-21 2.207983959725553 1104 22-23 2.529589832324703 1104 24-25 2.876534827377874 1104 26-27 1.9714052110148614 1104 28-29 2.3418877367047983 1104 30-31 1.9347493961102415 1104 32-33 2.027463804387942 1104 34-35 1.5075743061470774 1104 36-37 2.416528382129151 1104 38-39 2.369949470657719 1104 40-41 2.392055684017368 1104 42-43 2.409533138495121 1104 44-45 2.3913056680465594 1104 46-47 3.163391081518199 1104 48-49 3.297872757914508 1104 50-51 2.936683221194169 1104 52-53 2.3439334182619866 1104 54-55 3.2762842832030543 1104 56-57 3.3163760902248143 1104 58-59 2.714610143448855 1104 60-61 3.329747457802693 1104 62-63 3.743739999559704 1104 64-65 3.6693263032134844 1104 66-67 3.1648460385772843 1104 68-69 2.8762524059553236 1104 70-71 2.9080564363486943 1104 72-73 2.299363472286853 1104 74-75 2.6297234180543434 1104 76-77 2.330892454935398 1104 78-79 2.9023226867216145 1104 80-81 1.809031050722389 1104 82-83 1.6966164847448688 1104 84-85 1.5921950017659512 1104 86-87 1.420319177728448 1104 88-89 1.9865591320092193 1104 90-91 1.8873813225358766 1104 92-93 1.7898110739686786 1104 94-95 1.9561523805595655 1104 96-97 2.2014254608235113 1104 98-99 1.9681198240844289 1104 100-101 1.676818609879021 1105 1 -1.0617178543103414 1105 2 -1.1801250588578327 1105 3 -0.5577518184877732 1105 4 -0.4555462838152451 1105 5 -0.30285846855814214 1105 6 -0.6168141141296886 1105 7 -0.09179057184292816 1105 8 -1.1083815298997628 1105 9 -1.126876062732805 1105 10-11 -0.40268389740653276 1105 12-13 -0.20207413988913459 1105 14-15 -0.634753833830608 1105 16-17 -0.474411864218105 1105 18-19 -1.0872124529856961 1105 20-21 0.14926399286557057 1105 22-23 0.09721617863787912 1105 24-25 -0.22744197461053517 1105 26-27 0.32589982576217125 1105 28-29 0.25023487837837166 1105 30-31 0.09195734971421388 1105 32-33 0.042687499499784565 1105 34-35 -0.5011249482025448 1105 36-37 -0.45681461704235105 1105 38-39 -0.039226171430104984 1105 40-41 -0.012770330895637727 1105 42-43 -0.2211628018528451 1105 44-45 -0.6833629317877445 1105 46-47 -1.0941689847618328 1105 48-49 -1.2852879711658574 1105 50-51 -0.6585943264446037 1105 52-53 -0.5827442950768713 1105 54-55 -1.0216651782716752 1105 56-57 -1.7690630149947424 1105 58-59 -1.7283268905196607 1105 60-61 -1.5501199821310294 1105 62-63 -1.5111274403740182 1105 64-65 -1.6586562982778155 1105 66-67 -0.8670512273713449 1105 68-69 -0.6450814797116173 1105 70-71 -1.0556966705278619 1105 72-73 -1.2607028077462843 1105 74-75 -0.5776088106117676 1105 76-77 -0.564612930317292 1105 78-79 -0.20061848974897245 1105 80-81 -0.548674003130138 1105 82-83 -0.2555376163321803 1105 84-85 -0.46134683750496563 1105 86-87 -0.8824977236800002 1105 88-89 -0.488896543218619 1105 90-91 -0.920095893702733 1105 92-93 -0.2960422814579964 1105 94-95 -0.5797838249085387 1105 96-97 -0.27537652757748177 1105 98-99 0.20103200304051683 1105 100-101 0.34893957093950334 1106 1 0.4561289525582666 1106 2 0.45116955815933935 1106 3 0.4682948726177649 1106 4 0.3287485261403802 1106 5 1.1050134203093052 1106 6 0.7370350080003547 1106 7 0.41293642024659505 1106 8 -0.28935779847008547 1106 9 -0.07478268052172865 1106 10-11 0.25552563375303095 1106 12-13 0.3927551095802926 1106 14-15 0.05066001915029261 1106 16-17 0.02828927411876947 1106 18-19 0.46053981404111966 1106 20-21 0.19499040613973762 1106 22-23 0.031906512421400635 1106 24-25 -0.006623915571360328 1106 26-27 -0.34436835868699234 1106 28-29 -0.5806545678882742 1106 30-31 -0.15973086174632556 1106 32-33 -0.9067626259102113 1106 34-35 -0.8010477728214944 1106 36-37 -1.937520771778992 1106 38-39 -2.5604494012198025 1106 40-41 -3.1134842031703798 1106 42-43 -2.9415949839867466 1106 44-45 -2.5288192312282227 1106 46-47 -2.93904646884441 1106 48-49 -3.7090772823755813 1106 50-51 -3.5556616619645744 1106 52-53 -2.5098135599813673 1106 54-55 -1.987515072155528 1106 56-57 -3.2536279387840494 1106 58-59 -3.0878676970023946 1106 60-61 -3.5211792142359855 1106 62-63 -3.9684880423419884 1106 64-65 -3.892015356738167 1106 66-67 -4.211060488417072 1106 68-69 -4.05102398405273 1106 70-71 -4.174739695802796 1106 72-73 -4.206680041008877 1106 74-75 -3.2383265416555638 1106 76-77 -3.0773468681911105 1106 78-79 -3.3787006815297964 1106 80-81 -3.4534588767554517 1106 82-83 -3.308016875448523 1106 84-85 -2.6566970207964964 1106 86-87 -2.9974890414496347 1106 88-89 -1.7065311177893285 1106 90-91 -2.56537854853584 1106 92-93 -3.4018690227275314 1106 94-95 -2.063287587208368 1106 96-97 -2.888824337726046 1106 98-99 -3.234237146100906 1106 100-101 -3.2701999235617514 1107 1 0.001852758556044165 1107 2 -0.9081418080013428 1107 3 -0.49893948502755237 1107 4 -1.1125695042187473 1107 5 -0.2398588492204965 1107 6 -0.7553752104372613 1107 7 -0.6962823876023485 1107 8 -1.092393711095042 1107 9 -0.5092513958123632 1107 10-11 -1.121279253325831 1107 12-13 -1.3691849126337061 1107 14-15 -0.09012117300076028 1107 16-17 -0.443387882489894 1107 18-19 -1.4591195713716942 1107 20-21 -1.188478679384147 1107 22-23 -0.8136136652905535 1107 24-25 -1.8702855223836607 1107 26-27 -2.003753771497067 1107 28-29 -1.2191218022459225 1107 30-31 -0.9730407469740214 1107 32-33 -2.1527826185055403 1107 34-35 -1.1712810197670684 1107 36-37 -0.975562237902647 1107 38-39 -1.0233335182135086 1107 40-41 -0.6280158581129953 1107 42-43 -0.2669374497401691 1107 44-45 -0.27224759109493846 1107 46-47 0.3786136570348937 1107 48-49 0.6112670345440776 1107 50-51 0.4430425216711207 1107 52-53 0.5386869953685043 1107 54-55 1.5153542355083012 1107 56-57 1.0781010504791837 1107 58-59 1.8849201593471072 1107 60-61 2.269731559551502 1107 62-63 1.6735889661733765 1107 64-65 1.5669813111626105 1107 66-67 2.015799933648829 1107 68-69 1.956936030756598 1107 70-71 1.8762599339639578 1107 72-73 1.2449119619529903 1107 74-75 0.8602480603436966 1107 76-77 1.7543821210721227 1107 78-79 1.2588409697104872 1107 80-81 1.6023537852215952 1107 82-83 1.2343748313267433 1107 84-85 1.604317418300134 1107 86-87 1.670319177728448 1107 88-89 1.7633874308963406 1107 90-91 1.5473574751590888 1107 92-93 1.4088492297715405 1107 94-95 2.0358423646613133 1107 96-97 2.772967591189172 1107 98-99 2.232626978297464 1107 100-101 0.9935912648869696 1108 1 -1.3103094036061158 1108 2 -0.9753715377310712 1108 3 -0.4445475931356597 1108 4 -0.9162857204349635 1108 5 -0.45303452489617513 1108 6 -1.213821156383208 1108 7 -1.5567553605753197 1108 8 -0.9765153327166622 1108 9 -1.1754676120285836 1108 10-11 -1.0405360100825902 1108 12-13 -0.9783065342553243 1108 14-15 -1.3421482000277933 1108 16-17 -1.4758203149223235 1108 18-19 -1.375504706506824 1108 20-21 -0.975471922627392 1108 22-23 -0.4990866382635346 1108 24-25 -1.155082819680949 1108 26-27 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64-65 -0.7595952654139673 2314 66-67 -0.5495153816664846 2314 68-69 -0.6016225278019611 2314 70-71 -0.3331995254955018 2314 72-73 -0.15373668669565532 2314 74-75 -0.8221843720887385 2314 76-77 -0.8176899509999451 2314 78-79 -0.5654833546138356 2314 80-81 -1.0890876562198457 2314 82-83 -0.7070666101146976 2314 84-85 -0.9812681672854531 2314 86-87 -0.8634646060553344 2314 88-89 -1.2073332898243798 2314 90-91 -1.0247145969129825 2314 92-93 -0.809619238696925 2314 94-95 -0.5339774551585101 2314 96-97 -1.015320697099117 2314 98-99 -0.27187752620703876 2314 100-101 -0.155057383761676 2315 1 -1.0421275854243 2315 2 -0.6833260831856194 2315 3 -1.8343203204083878 2315 4 -2.337876629525873 2315 5 -2.451898161259809 2315 6 -1.4649575200195706 2315 7 -1.0238008151207758 2315 8 -0.6958335145348471 2315 9 -1.2618312483922196 2315 10-11 -0.7234905555371327 2315 12-13 -1.431715625164415 2315 14-15 -0.7398754727550667 2315 16-17 -0.25763849674051187 2315 18-19 -0.516981979234096 2315 20-21 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2315 86-87 -1.079680822271552 2315 88-89 -0.8234676059586938 2315 90-91 -1.4910946132929972 2315 92-93 -1.3269001559778424 2315 94-95 -1.0594893306703845 2315 96-97 -1.3025852343636544 2315 98-99 -1.3635646678941828 2315 100-101 -1.4996519783562725 2316 1 -0.2563094036061173 2316 2 -0.08987153773107437 2316 3 -0.2510475931356595 2316 4 -0.09678572043496558 2316 5 0.33646547510382874 2316 6 -0.3813211563832084 2316 7 -0.42125536057532287 2316 8 -0.13001533271665977 2316 9 0.07053238797141859 2316 10-11 -1.056036010082586 2316 12-13 -0.10680653425532682 2316 14-15 0.004851799972207971 2316 16-17 0.0601796850776708 2316 18-19 -0.16625470650682672 2316 20-21 -0.64172192262739 2316 22-23 -0.34058663826353097 2316 24-25 -0.46158281968094883 2316 26-27 -0.4388889066321937 2316 28-29 -0.23922991035402674 2316 30-31 -0.7846623685956402 2316 32-33 -0.32424207796500326 2316 34-35 -0.5503080467941004 2316 36-37 0.7834107350703263 2316 38-39 0.026125941245950912 2316 40-41 0.1711733310761936 2316 42-43 -0.6769374497401692 2316 44-45 -0.7105178613652079 2316 46-47 -0.24019715377591666 2316 48-49 0.4284021696792166 2316 50-51 0.9124479270765242 2316 52-53 0.679227535909046 2316 54-55 0.3368136949677627 2316 56-57 0.48631726669540143 2316 58-59 0.7424336728606207 2316 60-61 0.010218046037984152 2316 62-63 -0.05078941220500255 2316 64-65 0.20573806791936633 2316 66-67 -0.23538925554035828 2316 68-69 -0.0699828881623219 2316 70-71 -0.28076709306306924 2316 72-73 -0.14087182183078895 2316 74-75 0.10419400628964226 2316 76-77 0.19859833728834175 2316 78-79 0.21938151025102925 2316 80-81 0.29667810954591545 2316 82-83 0.18496942592134147 2316 84-85 0.08237147235418618 2316 86-87 0.13631917772844915 2316 88-89 0.3914414849503949 2316 90-91 0.573087204888818 2316 92-93 0.647281662203973 2316 94-95 0.06432885114779907 2316 96-97 0.4477784019999831 2316 98-99 0.9577080593785432 2316 100-101 0.9023480216437285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 14.0 7 500.0 8 1310.0 9 1164.0 10 1279.0 11 1408.0 12 1579.0 13 1423.0 14 1295.0 15 1209.0 16 1613.0 17 2298.0 18 2376.0 19 2928.0 20 4095.0 21 4742.0 22 3590.0 23 3272.0 24 3140.0 25 2389.0 26 2240.0 27 1984.0 28 1880.0 29 1440.0 30 1226.0 31 1333.0 32 1339.0 33 1754.0 34 2206.0 35 3193.0 36 4600.0 37 7730.0 38 7100.0 39 1681.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.57782395396589 18.49971105728443 12.873320136233415 13.049144852516262 2 15.710263251730625 25.994417735371144 40.167713853606955 18.127605159291274 3 22.78354951742792 38.27011741562673 26.46216266060122 12.484170406344132 4 11.991147722382738 19.605335956230405 34.64683100756132 33.756685313825535 5 9.805247497971328 46.263555216760516 27.378464110949913 16.55273317431824 6 23.273742192494957 36.314119903604976 20.142133477597994 20.270004426302073 7 22.760749019463194 41.21574268747003 19.738605486088055 16.284902806978717 8 31.203816409083647 29.196021295169245 19.052537100561885 20.547625195185226 9 34.726850201035305 15.161873378499674 18.548119367015456 31.56315705344957 10-11 23.834553647725663 33.62411857644332 20.789607962474562 21.75171981335645 12-13 30.402975440322134 30.460148157255713 23.47524052500538 15.661635877416776 14-15 28.797565623655252 27.903731480912274 27.615417716850065 15.683285178582407 16-17 18.96416057048011 32.267166656420976 29.2168193274728 19.551853445626115 18-19 19.360054097252107 27.438986906006026 37.60127866232249 15.599680334419377 20-21 21.867238793124645 27.237686576339538 35.914254801190154 14.980819829345663 22-23 19.2168193274728 29.900411876805805 33.0909202680273 17.791848527694103 24-25 18.11039595743504 31.047095635923256 32.56428698768665 18.278221418955056 26-27 18.958977543078806 29.021866758469756 33.54398863936755 18.475167059083894 28-29 18.655519011465284 30.578796913902806 35.18704085082839 15.578643223803523 30-31 18.878724162947115 33.28291957160599 33.94813530561806 13.89022095982884 32-33 14.918546751091167 34.046843302391345 35.846191676400075 15.188418270117415 34-35 13.964467941230712 34.42675355013217 35.09989549394479 16.508883014692323 36-37 15.427552714083728 35.186574045613824 33.644802360607365 15.741070879695087 38-39 16.601094239872133 33.72103030675601 32.673510788713344 17.00436466465851 40-41 15.660539743038052 35.46935513616524 32.455277555787795 16.414827565008913 42-43 16.425278170529293 36.10253888239995 31.625991270670685 15.846191676400073 44-45 16.10745681440954 35.3427183869183 31.90692813671851 16.64289666195365 46-47 15.036577119321326 35.85787176492285 31.676400073768978 17.429151041986845 48-49 15.912262474871977 34.658535535787834 32.00833604849171 17.420865940848483 50-51 14.560331472692848 34.93250055327415 31.558757715100693 18.948410258932306 52-53 14.306264215897215 35.489641605704804 29.664965881846683 20.5391282965513 54-55 14.699084035163215 34.18700436466466 29.282596668101064 21.831314932071063 56-57 15.394356673018997 31.782750353476363 29.163336816868508 23.659556156636135 58-59 15.67099034855843 30.265568328517855 29.48546136349665 24.57797995942706 60-61 16.059506977316037 28.733632507530583 30.263724104014262 24.943136411139115 62-63 15.390762837418315 27.503703840313765 31.307747634767537 25.797785687500387 64-65 15.74812346696011 26.645847037198696 32.4048516294024 25.2011778664388 66-67 17.195972114639815 25.550514551289144 32.569805613957435 24.683707720113606 68-69 16.554467376135058 25.06255494691283 32.8660924522154 25.51688522473672 70-71 15.581948619589472 25.34287418008348 33.641320718760184 25.433856481566863 72-73 16.50580930718633 24.223888854736582 33.88578102907727 25.384520808999817 74-75 16.765844962193395 22.543800331960412 34.129218663551974 26.561136042294216 76-77 16.071801807340012 22.839490994037007 35.15952541956107 25.929181779061906 78-79 17.285301530706338 22.023729021946274 35.78164381877421 24.909325628573185 80-81 16.579463500710126 22.92326326338635 35.24872884221658 25.248544393686938 82-83 16.480172148785734 23.859821703043345 33.45588687365509 26.20411927451583 84-85 16.424921758271488 24.9432785897428 32.71211347971249 25.91968617227322 86-87 17.639246693678793 24.029315740609803 31.349643083317453 26.981794482393955 88-89 16.9969441899644 23.200177077120777 31.023542649147508 28.779336083767316 90-91 18.54072133889155 22.832355726073217 30.283690559634042 28.34323237540119 92-93 19.57725956484049 22.316833996298868 29.340989714299763 28.764916724560873 94-95 19.41154768492898 21.916620549714075 28.394515157105083 30.27731660825186 96-97 20.412040281825224 21.076641213865013 27.317496034527277 31.193822469782482 98-99 21.563925677254336 20.946004107180187 26.507298237847543 30.98277197771793 100-101 21.534280602300747 20.57082075465898 25.916270604944575 31.978628038095707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 110.0 1 67.5 2 64.5 3 103.0 4 74.5 5 47.5 6 68.5 7 133.0 8 177.0 9 165.0 10 129.0 11 105.5 12 98.0 13 99.5 14 124.0 15 139.5 16 149.0 17 153.5 18 169.5 19 206.0 20 249.5 21 343.5 22 415.5 23 509.0 24 677.5 25 866.0 26 1116.0 27 1377.5 28 1681.0 29 2114.5 30 2511.5 31 2740.0 32 3149.0 33 3489.0 34 3294.0 35 3148.0 36 3291.0 37 3132.5 38 2809.5 39 2631.5 40 2157.0 41 1645.5 42 1492.5 43 1447.5 44 1320.0 45 1255.5 46 1439.5 47 1701.0 48 1990.5 49 2387.0 50 3644.5 51 3838.0 52 2702.5 53 2082.0 54 1587.0 55 1460.0 56 1211.5 57 850.5 58 710.0 59 603.0 60 503.0 61 435.0 62 402.5 63 344.0 64 290.5 65 259.5 66 254.5 67 224.0 68 190.5 69 168.5 70 130.5 71 117.0 72 101.0 73 81.0 74 76.0 75 63.5 76 46.5 77 40.5 78 40.0 79 30.5 80 20.5 81 19.5 82 23.0 83 18.5 84 12.5 85 10.0 86 7.5 87 6.0 88 4.0 89 4.0 90 4.0 91 3.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004917932009589968 2 0.0073768980143849505 3 0.0 4 0.0 5 0.001229483002397492 6 0.0036884490071924752 7 0.002458966004794984 8 0.002458966004794984 9 0.0073768980143849505 10-11 0.004303190508391222 12-13 0.0030737075059937297 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.001229483002397492 22-23 0.0 24-25 6.14741501198746E-4 26-27 0.0018442245035962376 28-29 0.0030737075059937297 30-31 0.009835864019179935 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0018442245035962376 50-51 0.001229483002397492 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.14741501198746E-4 64-65 0.0018442245035962376 66-67 0.002458966004794984 68-69 0.007991639515583697 70-71 6.14741501198746E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014139054527571157 82-83 0.012294830023974919 84-85 0.020286469539558618 86-87 0.01659802053236614 88-89 0.01782750353476363 90-91 0.017212762033564886 92-93 0.010450605520378681 94-95 0.024589660047949838 96-97 0.009835864019179935 98-99 0.017212762033564886 100-101 0.01659802053236614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 81335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.0018442245036 #Duplication Level Percentage of deduplicated Percentage of total 1 87.91954559984264 43.96139423372472 2 5.230027785291008 5.23022069219893 3 2.0654552607637267 3.0982971660416796 4 1.0696107600383584 2.139300424171636 5 0.7081560894047063 1.7704555234523882 6 0.43767980525707545 1.3130878465605214 7 0.33440704221888906 1.1704678182824122 8 0.25818190759546583 1.0327657220138933 9 0.19671002483464062 0.8852277617261941 >10 1.4999139393641348 14.683715497633246 >50 0.14753251862598046 5.037191860822524 >100 0.11802601490078439 11.373947255179196 >500 0.007376625931299024 3.00485645785947 >1k 0.007376625931299024 5.29907174033319 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1838 2.25978975840659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1237 1.5208704739656975 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1235 1.5184115079609024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 1.1766152332943998 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 912 1.1212884981865125 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 575 0.7069527263785579 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 482 0.5926108071555911 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 463 0.5692506301100387 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 364 0.447531812872687 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 340 0.41802422081514723 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 291 0.3577795536976701 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.3504026556832852 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 278 0.3417962746665027 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.3295014446425278 No Hit GTAATAAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGT 268 0.3295014446425278 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 253 0.3110591996065654 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 251 0.30860023360177047 No Hit GACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCACATTT 243 0.2987643695825905 No Hit AAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAA 242 0.297534886580193 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 239 0.29384643757300055 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.29384643757300055 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 213 0.26187987951066577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 210 0.2581914305034733 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 208 0.2557324644986783 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 206 0.2532734984938833 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 202 0.24835556648429336 No Hit CCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCA 182 0.2237659064363435 No Hit CTTTTATCCAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAA 173 0.21270055941476607 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 165 0.20286469539558616 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 162 0.19917624638839368 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 159 0.19548779738120123 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 158 0.19425831437880373 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.1831929673572263 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 144 0.17704555234523883 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.17458658634044386 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.17458658634044386 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 139 0.17089813733325135 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 134 0.1647507223212639 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 134 0.1647507223212639 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.15983279031167394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.15737382430687896 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 126 0.15491485830208396 No Hit GGTTAAGCATTCAACAATTATTCTGTTTTAAAAAGTGCTTTAACAACTAC 123 0.1512264092948915 No Hit GTACATGGGGATTAACACAGGTGACGATATCAAGGCCAGTTTATTGCAGA 123 0.1512264092948915 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.1487674432900965 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.14384951128050655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 112 0.1377020962685191 No Hit TTATTAAACTCTTTGTAGCACAAAGGATAAATACTTGAGGTGTTCAATAC 110 0.13524313026372412 No Hit GTGCTAAAGTTATCCTGGGAGAATTAGGGTCAAGTCTTATGATGCCAGAG 108 0.1327841642589291 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 107 0.13155468125653164 No Hit GTGTATATATTTATGGGATACATGAGATGGTTTGACACAGGCATGCAATG 106 0.13032519825413413 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 106 0.13032519825413413 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 104 0.12786623224933916 No Hit GTACATGGGAAGATGCCAATTGCTGACTGGAAGTGCTGTGCTAAAGTTAT 102 0.12540726624454418 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 98 0.1204893342349542 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 95 0.11680088522776173 No Hit GTACATGGGAGTGAGAAAAGATATTTCATAATGATAAAAGACACGATATG 91 0.11188295321817175 No Hit GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 91 0.11188295321817175 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.11065347021577426 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 89 0.10942398721337676 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 88 0.10819450421097929 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 85 0.10450605520378681 No Hit TAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAA 85 0.10450605520378681 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 83 0.10204708919899182 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0018442245035962378 0.0 12-13 0.0 0.0 0.0 0.0061474150119874595 0.0 14-15 0.0 0.0 0.0 0.010450605520378681 0.0 16-17 0.0 0.0 0.0 0.017212762033564886 0.0 18-19 0.0 0.0 0.0 0.0227454355443536 0.0 20-21 0.0 0.0 0.0 0.03504026556832852 0.0 22-23 0.0 0.0 0.0 0.06331837462347083 0.0 24-25 0.0 0.0 0.0 0.1149566607241655 0.0 26-27 0.0 0.0 0.0 0.17827503534763633 0.0 28-29 0.0 0.0 0.0 0.2876990225610131 0.0 30-31 0.0 0.0 0.0 0.4684330239134444 0.0 32-33 0.0 0.0 0.0 0.7198622979037315 0.0 34-35 0.0 0.0 0.0 1.124976947193705 0.0 36-37 0.0 0.0 0.0 1.662261019241409 0.0 38-39 0.0 0.0 0.0 2.3071248539988938 0.0 40-41 0.0 0.0 0.0 3.3730866170775187 0.0 42-43 0.0 0.0 0.0 4.006885104813426 0.0 44-45 0.0 0.0 0.0 4.443351570664536 0.0 46-47 0.0 0.0 0.0 4.913014077580377 0.0 48-49 0.0 0.0 0.0 5.382061842995021 0.0 50-51 0.0 0.0 0.0 5.841888485891682 0.0 52-53 0.0 0.0 0.0 6.373025142927399 0.0 54-55 0.0 0.0 0.0 6.809491608778509 0.0 56-57 0.0 0.0 0.0 7.159894264461794 0.0 58-59 0.0 0.0 0.0 7.426077334480851 0.0 60-61 0.0 0.0 0.0 7.667056002950758 0.0 62-63 0.0 0.0 0.0 7.9609024405237605 0.0 64-65 0.0 0.0 0.0 8.227700252044016 0.0 66-67 0.0 0.0 0.0 8.466219954509128 0.0 68-69 0.0 0.0 0.0 8.796336140652855 0.0 70-71 0.0 0.0 0.0 9.223581483985985 0.0 72-73 0.0 0.0 0.0 9.537099649597344 0.0 74-75 0.0 0.0 0.0 9.854921005717095 0.0 76-77 0.0 0.0 0.0 10.213315300915966 0.0 78-79 0.0 0.0 0.0 10.545890453064487 0.0 80-81 0.0 0.0 0.0 10.865556033687835 0.0 82-83 0.0 0.0 0.0 11.258375852953833 0.0 84-85 0.0 0.0 0.0 11.619229114157497 0.0 86-87 0.0 0.0 0.0 11.939509436282044 0.0 88-89 0.0 0.0 0.0 12.317575459519272 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATAGG 30 1.3562767E-6 79.16308 3 AAGTACT 40 8.958341E-8 71.24677 4 AGTACTC 50 5.487891E-9 66.49699 5 AAAGTAC 45 2.0244988E-7 63.330463 3 ATAGGAG 25 0.00500545 56.997417 5 CATAGGA 50 4.1941348E-7 56.997417 4 TAATAAA 75 3.6379788E-11 56.997414 2 GTTAAGC 35 2.7731428E-4 54.283257 2 TTAAGCA 35 2.7731428E-4 54.283257 3 TAAATAA 80 4.143658E-9 47.497845 5 TAAGCAT 40 5.3597067E-4 47.497845 4 GTAATAA 95 3.7471182E-10 44.99796 1 ATTATTC 65 2.5604531E-6 43.844166 3 GGATTAA 65 2.5604531E-6 43.844166 9 GGGATTA 65 2.5604531E-6 43.844166 8 CATTCAA 45 9.5744425E-4 42.22031 8 ATTCAAC 45 9.5744425E-4 42.22031 9 GGTTAAG 45 9.5744425E-4 42.22031 1 AAAAGTA 70 4.2622014E-6 40.712444 2 ATAATAC 105 1.0022632E-9 40.71244 3 >>END_MODULE