FastQCFastQC Report
Fri 10 Feb 2017
SRR2568656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81335
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT51936.384705231450175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19832.4380647937542266No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT19152.354459949591197TruSeq Adapter, Index 10 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14621.7975041495051332No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG13931.712669822339706TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT13531.6634905022438065RNA PCR Primer, Index 10 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11941.4680027048626052No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9871.2134997233663245No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9851.2110407573615294No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5900.7253949714145203No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT4610.5667916641052437TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA4550.5594147660908588RNA PCR Primer, Index 10 (96% over 28bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4270.524989242023729No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4070.5003995819757792No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3800.46720354091104693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.44138439786069955No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC3330.4094178397983648No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA3260.4008114587815823No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.38728714575520995No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.3614680027048626No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2800.34425524067129776No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG2770.34056679166410525TruSeq Adapter, Index 10 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2520.3098297166041679No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2450.3012233355873855No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2400.29507592057539805No Hit
GACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCACATTT2390.29384643757300055No Hit
CTTTTATCCAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAA2300.28278109055142314No Hit
GTAATAAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGT2210.2717157435298457No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA2190.26925677752505073No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC2140.26310936251306327Illumina Paired End PCR Primer 2 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2120.26065039650826827No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2070.2545029814962808No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA2020.24835556648429336No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1880.23114280445072846No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1800.22130694043154853No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1750.21515952541956107No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1710.21024159340997112No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1690.20778262740517614No Hit
AAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAA1680.2065531444027786No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT1670.20532366140038114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.20532366140038114No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.20286469539558616No Hit
CCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCA1610.19794676338599618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1967172803835987No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1600.1967172803835987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1540.18934038236921374No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1440.17704555234523883No Hit
TTATTAAACTCTTTGTAGCACAAAGGATAAATACTTGAGGTGTTCAATAC1350.1659802053236614No Hit
GTACATGGGAAGATGCCAATTGCTGACTGGAAGTGCTGTGCTAAAGTTAT1300.15983279031167394No Hit
GTGTATATATTTATGGGATACATGAGATGGTTTGACACAGGCATGCAATG1280.15737382430687896No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1280.15737382430687896No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.15491485830208396No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1150.14139054527571157No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.14016106227331407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1130.13893157927091657No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1377020962685191No Hit
GGTTAAGCATTCAACAATTATTCTGTTTTAAAAAGTGCTTTAACAACTAC1120.1377020962685191No Hit
GTGCTAAAGTTATCCTGGGAGAATTAGGGTCAAGTCTTATGATGCCAGAG1110.1364726132661216No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.1327841642589291No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1080.1327841642589291No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1030.12663674924694165No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.12417778324214668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1010.12417778324214668No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA990.12171881723735169No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA990.12171881723735169No Hit
GTTTTAAAAAGTGCTTTAACAACTACCTACCCTGTTCTTTTCTTAAAAGC970.11925985123255672No Hit
GTACATGGGGATTAACACAGGTGACGATATCAAGGCCAGTTTATTGCAGA930.11434191922296674No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA920.11311243622056925No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.11311243622056925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG910.11188295321817175No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT890.10942398721337676No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT880.10819450421097929No Hit
ATATTAAAGTTTTGTTATTACAAGGGGAAAGGCTCGTGAAAATATATTTT860.1057355382061843No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT860.1057355382061843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC840.1032765722013893No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA840.1032765722013893No Hit
GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA830.10204708919899182No Hit
CAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAACCTAACCA830.10204708919899182No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA830.10204708919899182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAAGT601.4700327E-647.5236934
GGATTAA502.8056265E-547.494469
ATGGGAC554.9080612E-543.2033545
GGGATTA554.925939E-543.1767858
TTATTAA459.491041E-442.2953381
AAGTTTT459.548811E-442.2432827
TAAATAA608.1987484E-539.6030775
GGGGATT756.8198897E-638.0189557
ATGGGCA500.001603059538.018955
AAAGTAC500.001603059538.018953
AGTACTC500.001603059538.018955
CCTTTGT250.001612661437.9955782-83
GTAATAA651.3039939E-436.6017341
ATGGGGA1301.9826984E-1036.5566865
TGGGGAT651.313491E-436.5566866
ATAATAC651.3182609E-436.5342038
AAGTACT550.002559333634.5626834
GTTTTGT550.00256707634.5414289
CTTATAC16300.034.1541371
TATACAC16600.033.2093283
Produced by FastQC (version 0.11.5)