##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568656_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81335 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.168205569558 33.0 31.0 34.0 30.0 34.0 2 32.211987459273374 33.0 31.0 34.0 30.0 34.0 3 32.185135550501016 34.0 31.0 34.0 30.0 34.0 4 35.819487305588 37.0 35.0 37.0 35.0 37.0 5 35.920956537775865 37.0 35.0 37.0 35.0 37.0 6 36.023729021946274 37.0 35.0 37.0 35.0 37.0 7 35.89834634536177 37.0 35.0 37.0 35.0 37.0 8 35.928972766951496 37.0 35.0 37.0 35.0 37.0 9 37.66731419438126 39.0 37.0 39.0 35.0 39.0 10-11 37.569047765414645 39.0 37.0 39.0 35.0 39.0 12-13 37.47890207167886 39.0 37.0 39.0 34.5 39.0 14-15 38.484920390975596 40.0 38.0 41.0 34.0 41.0 16-17 38.402206921989304 40.0 38.0 41.0 33.5 41.0 18-19 38.70050408803098 40.0 38.0 41.0 34.0 41.0 20-21 38.799790987889594 40.0 38.0 41.0 35.0 41.0 22-23 38.700854490686666 40.0 38.0 41.0 35.0 41.0 24-25 38.578902071678854 40.0 38.0 41.0 34.0 41.0 26-27 38.227097805372836 40.0 37.0 41.0 34.0 41.0 28-29 37.79359439355751 40.0 36.0 41.0 33.0 41.0 30-31 37.257041863896234 39.0 35.0 41.0 33.0 41.0 32-33 36.40438925431856 38.0 35.0 41.0 31.0 41.0 34-35 35.94443351570665 38.0 35.0 41.0 30.0 41.0 36-37 35.47316653347268 38.0 35.0 41.0 27.0 41.0 38-39 34.5656912768181 37.0 34.5 40.0 22.0 41.0 40-41 34.15647630171513 36.0 34.0 40.0 21.0 41.0 42-43 33.53428413352185 36.0 33.5 40.0 16.0 41.0 44-45 32.63684145816684 35.5 32.5 40.0 10.5 41.0 46-47 31.406319542632325 35.0 30.0 40.0 7.5 41.0 48-49 30.29100018442245 35.0 25.5 39.0 7.0 41.0 50-51 29.177371365340875 35.0 20.0 39.0 7.0 41.0 52-53 29.065709719063136 35.0 19.0 39.0 7.0 41.0 54-55 28.572434991086247 35.0 16.5 39.0 7.0 41.0 56-57 27.831665334726747 34.5 11.5 39.0 7.0 41.0 58-59 27.132722690108807 34.0 10.0 38.5 7.0 41.0 60-61 26.29462715927952 33.5 10.0 38.0 7.0 41.0 62-63 25.47435298456999 33.0 8.0 37.0 7.0 41.0 64-65 24.74410770271101 31.5 8.0 37.0 7.0 40.5 66-67 23.690157988565808 28.0 7.5 36.0 7.0 40.0 68-69 22.446867892051394 22.5 7.0 35.5 7.0 39.0 70-71 21.642982725763815 20.0 7.0 35.0 7.0 39.0 72-73 21.029581361037685 19.0 7.0 35.0 7.0 38.0 74-75 20.68327288375238 18.5 7.0 35.0 7.0 37.0 76-77 19.614237413167764 16.0 7.0 33.5 7.0 35.5 78-79 20.205827749431364 18.0 7.0 35.0 7.0 36.0 80-81 20.178785270793632 18.0 7.0 35.0 7.0 36.0 82-83 20.07175877543493 17.5 7.0 35.0 7.0 35.0 84-85 19.922726993299317 17.0 7.0 35.0 7.0 35.0 86-87 19.78744083113051 17.0 7.0 35.0 7.0 35.0 88-89 19.67809061289728 17.0 7.0 35.0 7.0 35.0 90-91 19.550716173848897 15.0 7.0 35.0 7.0 35.0 92-93 19.424276141882338 15.0 7.0 34.0 5.0 35.0 94-95 19.421749554312413 15.0 7.0 34.0 5.0 35.0 96-97 19.387385504395404 15.0 7.0 34.0 5.0 35.0 98-99 19.349394479621317 15.0 7.0 34.0 5.0 35.0 100-101 18.69883199114772 15.0 7.0 33.0 5.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6074060913634369 1101 2 0.40061140531772566 1101 3 0.6734668168212892 1101 4 0.5065277881652648 1101 5 0.6144787098893119 1101 6 0.516942213594767 1101 7 0.5206550060272832 1101 8 0.5605053219212266 1101 9 0.8719357573220989 1101 10-11 0.5271246019533251 1101 12-13 0.4081867819220264 1101 14-15 0.7496889031254668 1101 16-17 1.0435810328341475 1101 18-19 0.15953391636391956 1101 20-21 0.7155825725123464 1101 22-23 0.42135943329657266 1101 24-25 0.8993077339227682 1101 26-27 0.6139927920426089 1101 28-29 1.0530145987699413 1101 30-31 1.3636166628197657 1101 32-33 1.2087269793357365 1101 34-35 2.0717001942957793 1101 36-37 2.370215514726141 1101 38-39 2.408667934338382 1101 40-41 1.808610305977588 1101 42-43 2.471675376407582 1101 44-45 2.640624730408163 1101 46-47 2.953532622317038 1101 48-49 3.6533632398451488 1101 50-51 3.7629590504281936 1101 52-53 3.2843247898897125 1101 54-55 2.729360654354295 1101 56-57 3.028808425575452 1101 58-59 3.3033621284328696 1101 60-61 3.3588161803969996 1101 62-63 2.609523221670429 1101 64-65 2.807026580296398 1101 66-67 2.345733840621719 1101 68-69 2.6083166321724107 1101 70-71 2.39812090055306 1101 72-73 2.136077116770732 1101 74-75 1.5447159019814194 1101 76-77 1.6389171441998371 1101 78-79 1.8008192086058052 1101 80-81 1.9657559520411994 1101 82-83 1.5071225707192077 1101 84-85 2.21569738960115 1101 86-87 2.0889348258231664 1101 88-89 1.3955991174381062 1101 90-91 1.2889279615785192 1101 92-93 1.755253583370454 1101 94-95 1.090610024467093 1101 96-97 1.6279830848512766 1101 98-99 1.7264276933808773 1101 100-101 1.4573196465524454 1102 1 0.08750559385099876 1102 2 -0.07179926858547958 1102 3 0.436469982809534 1102 4 0.1137191043118051 1102 5 -0.4682892684010582 1102 6 0.15059214575216373 1102 7 0.5437214917803388 1102 8 0.3884112468420753 1102 9 0.3264812118675522 1102 10-11 -0.3332327024338255 1102 12-13 0.2669610921888719 1102 14-15 0.559051182636999 1102 16-17 0.4915683688811825 1102 18-19 -0.1671282274624062 1102 20-21 -0.106217517944458 1102 22-23 0.008311038904899704 1102 24-25 -0.08633224798587236 1102 26-27 0.28665222216472586 1102 28-29 0.3079897231480473 1102 30-31 -0.33290075011556297 1102 32-33 -0.7262571907230608 1102 34-35 -0.5782319630990642 1102 36-37 -0.7182060139938926 1102 38-39 -0.3748010842052665 1102 40-41 -0.7201097302051309 1102 42-43 -0.74383796597143 1102 44-45 -0.38515545955113595 1102 46-47 -0.5434144604509434 1102 48-49 0.746420679917513 1102 50-51 1.244867689053244 1102 52-53 1.4177485800299188 1102 54-55 0.5577188633095176 1102 56-57 0.10309607822131284 1102 58-59 -0.27341308640204787 1102 60-61 -1.0049332542479554 1102 62-63 -1.403027660283442 1102 64-65 -1.1911642835661098 1102 66-67 -0.2569346351810857 1102 68-69 -0.40525188885879615 1102 70-71 -0.02589538167217853 1102 72-73 0.5234583922117082 1102 74-75 1.4385422249122222 1102 76-77 1.5030057918705708 1102 78-79 2.518028616113721 1102 80-81 1.2041096381560799 1102 82-83 0.8955214852375271 1102 84-85 1.0440555985563726 1102 86-87 0.25774984165310855 1102 88-89 0.5082178419971299 1102 90-91 0.3003481334464517 1102 92-93 0.6505498294129666 1102 94-95 0.5544273017172081 1102 96-97 1.0328903666242297 1102 98-99 0.9816289597761703 1102 100-101 0.5176995651413208 1103 1 0.25168469832861007 1103 2 0.017752970220485054 1103 3 -0.6978583753994236 1103 4 -0.06538537330013838 1103 5 0.14365103010640468 1103 6 0.031189160677534744 1103 7 0.00640805894451546 1103 8 -0.2235290516653876 1103 9 -0.13620535529662448 1103 10-11 -0.19144165765771248 1103 12-13 -0.0763224899006758 1103 14-15 -0.314083145721213 1103 16-17 -0.6278346161934465 1103 18-19 0.24331953373162207 1103 20-21 0.12512576563762678 1103 22-23 0.30681850159146506 1103 24-25 0.5256080505215905 1103 26-27 0.11501043111994846 1103 28-29 0.2557509171778989 1103 30-31 -0.07916940683197993 1103 32-33 0.04239952569485439 1103 34-35 0.5187829622740665 1103 36-37 1.3191074188419307 1103 38-39 1.7222138411678714 1103 40-41 1.2425768369590529 1103 42-43 1.6740724817897643 1103 44-45 1.0029042419414083 1103 46-47 0.1655407634296573 1103 48-49 0.30612217245482753 1103 50-51 0.15531545024727222 1103 52-53 0.07446499794036754 1103 54-55 -0.41989307698899125 1103 56-57 -0.5237695934204787 1103 58-59 -1.5047563699841362 1103 60-61 -2.751201910964376 1103 62-63 -2.261236615507322 1103 64-65 -2.4150448805810356 1103 66-67 -1.5927555307034744 1103 68-69 -1.3679384560229764 1103 70-71 -0.8691789637617298 1103 72-73 -0.939228174952472 1103 74-75 -0.44951747658031493 1103 76-77 -0.3328151036518179 1103 78-79 -0.1536131749310563 1103 80-81 -0.9451440931872028 1103 82-83 -0.4701501565535189 1103 84-85 -1.3141533566675072 1103 86-87 -0.9437426956603225 1103 88-89 -0.6186478296446616 1103 90-91 -0.4683085829714564 1103 92-93 -0.7001964392437472 1103 94-95 -0.6769159818648838 1103 96-97 -1.1984529169578622 1103 98-99 -1.3915053685820347 1103 100-101 -1.8106586438139054 1104 1 0.14171981685363022 1104 2 0.11674331262610593 1104 3 0.11820834979811679 1104 4 0.011875381748147618 1104 5 0.03961239972888109 1104 6 0.1626641387284522 1104 7 0.22787425736418498 1104 8 0.2360846445593694 1104 9 -0.08528349401480284 1104 10-11 -0.3815206743390078 1104 12-13 -0.09069913608153257 1104 14-15 0.04313810098642534 1104 16-17 0.38226195975037314 1104 18-19 -0.09447677882324967 1104 20-21 0.08880004131805208 1104 22-23 -0.3484712262399654 1104 24-25 -0.0650416421913107 1104 26-27 0.22629225728325508 1104 28-29 -0.31218147966144016 1104 30-31 -0.2662852979645578 1104 32-33 0.2559640559845775 1104 34-35 -0.9149308217469994 1104 36-37 -0.6937327918692233 1104 38-39 -0.16463866102703406 1104 40-41 0.4137796464410499 1104 42-43 0.28963437819011517 1104 44-45 0.4133520031354365 1104 46-47 0.8566074886272013 1104 48-49 1.2117411364583397 1104 50-51 2.6464260736010914 1104 52-53 2.1648951998718857 1104 54-55 1.354360654354295 1104 56-57 -0.4792129647988794 1104 58-59 0.012363910964062086 1104 60-61 0.7302511180084075 1104 62-63 1.4303788366436905 1104 64-65 1.4135328191555772 1104 66-67 1.680181255951485 1104 68-69 1.2654021936697362 1104 70-71 1.834618226756266 1104 72-73 2.061210806610301 1104 74-75 2.019314832462701 1104 76-77 0.7019741851980541 1104 78-79 0.6700259822243453 1104 80-81 1.6928058629146392 1104 82-83 1.515143961093539 1104 84-85 1.3921679778364435 1104 86-87 1.6348350040762867 1104 88-89 1.316053662892653 1104 90-91 1.6055500649653283 1104 92-93 2.041572656454232 1104 94-95 2.2104852472834935 1104 96-97 2.7200062577568005 1104 98-99 2.2660890124539605 1104 100-101 1.1498330155364052 1105 1 -0.08508217154736286 1105 2 0.0589554087321531 1105 3 -0.4588421887271501 1105 4 -0.191935720157403 1105 5 -0.198581469368051 1105 6 -0.05668134594428409 1105 7 -0.14515805415197036 1105 8 -0.16698080697335627 1105 9 -0.1946455486958314 1105 10-11 -0.5252654962114178 1105 12-13 -0.37083583864988867 1105 14-15 0.16710247546861723 1105 16-17 0.5516902944645352 1105 18-19 -0.254998734084225 1105 20-21 -0.3379812345725952 1105 22-23 -0.23175623038247295 1105 24-25 0.04452753759327521 1105 26-27 -0.726276094000923 1105 28-29 -0.3636520678967301 1105 30-31 -0.3030500038469057 1105 32-33 -0.3507053723501343 1105 34-35 0.08962593053137624 1105 36-37 0.25583224541330196 1105 38-39 0.31765214261836405 1105 40-41 0.060424219763341114 1105 42-43 -0.009972084113115898 1105 44-45 -0.39650715179414675 1105 46-47 -1.1588233315882128 1105 48-49 -1.0484079936162232 1105 50-51 -1.1956410349324607 1105 52-53 -1.3255770453642377 1105 54-55 -2.538244979448521 1105 56-57 -2.4798343401095657 1105 58-59 -2.4462110683234286 1105 60-61 -1.8645086168714577 1105 62-63 -0.7595548833231724 1105 64-65 -1.2971134111675369 1105 66-67 -1.483758263518272 1105 68-69 -2.1528869529748356 1105 70-71 -1.7715333888195417 1105 72-73 -0.9586732033317027 1105 74-75 -2.047793701091564 1105 76-77 -1.4319321942551397 1105 78-79 -1.2729110517441704 1105 80-81 -0.8180682050223957 1105 82-83 -0.6743755086661949 1105 84-85 -0.5519082442016661 1105 86-87 -1.8328534797679552 1105 88-89 -1.2983829568256589 1105 90-91 -0.22761066411503705 1105 92-93 -0.6120106078373979 1105 94-95 -0.8829281744232169 1105 96-97 -0.46153889551577265 1105 98-99 -1.041284641232867 1105 100-101 -0.9127187657394877 1106 1 0.37681248405749557 1106 2 -0.40158867770427165 1106 3 0.10974743118409691 1106 4 0.2431404905313883 1106 5 0.20601046580463134 1106 6 0.35504931197584 1106 7 0.6003561268242947 1106 8 0.5096543464127166 1106 9 0.7922346365250874 1106 10-11 0.5818153450002299 1106 12-13 0.4255176245953365 1106 14-15 0.7235369728639469 1106 16-17 0.565581863054156 1106 18-19 0.3827593210961666 1106 20-21 0.6344285667008052 1106 22-23 0.9799937213829182 1106 24-25 0.5943056583727468 1106 26-27 0.682174610224429 1106 28-29 0.6842931375827206 1106 30-31 0.27914177697500975 1106 32-33 -0.09274666117899244 1106 34-35 -0.5559461319806829 1106 36-37 -0.34169814239299967 1106 38-39 -0.926222061510515 1106 40-41 -1.0813855429223693 1106 42-43 -0.18004733010964458 1106 44-45 -1.148291832481 1106 46-47 -2.0200041149183328 1106 48-49 -1.2898704670871908 1106 50-51 -1.2505320247067218 1106 52-53 -1.3584730515382653 1106 54-55 -0.8459818113991275 1106 56-57 -0.8432339156449729 1106 58-59 -0.7035909641366622 1106 60-61 -0.765820598349368 1106 62-63 -0.820426965129073 1106 64-65 -0.8388845861627132 1106 66-67 -1.3207252710428712 1106 68-69 -2.2733770166445275 1106 70-71 -1.8808330222364766 1106 72-73 -2.6491034560810753 1106 74-75 -1.6716601876823383 1106 76-77 -1.768513324874469 1106 78-79 -1.7146436390488198 1106 80-81 -1.2426292700426558 1106 82-83 -1.353711800389135 1106 84-85 -1.8938916514947408 1106 86-87 -1.5593632231598136 1106 88-89 -1.4356586658744703 1106 90-91 -1.1240027150507892 1106 92-93 -0.3078431372605124 1106 94-95 -1.00486527648766 1106 96-97 -0.571996159648517 1106 98-99 -0.010703896490436904 1106 100-101 -0.17530799977383182 1107 1 0.19299167694902053 1107 2 0.4653124700187945 1107 3 0.14603028938476825 1107 4 0.012670294307767449 1107 5 0.33929443470503884 1107 6 0.2822984789510343 1107 7 0.3363798217521037 1107 8 0.4356076970235989 1107 9 0.38053526592160836 1107 10-11 0.0588608836896114 1107 12-13 0.34471421844946093 1107 14-15 0.4026373060738706 1107 16-17 0.5317055209586314 1107 18-19 0.9353324421624407 1107 20-21 1.1820035389333157 1107 22-23 0.7471170090541506 1107 24-25 0.8208884055034318 1107 26-27 0.6078502859001063 1107 28-29 0.8052668510221892 1107 30-31 0.5753283745314732 1107 32-33 0.848571369180128 1107 34-35 0.5465159191115063 1107 36-37 0.4459730904837187 1107 38-39 1.3959734216438662 1107 40-41 1.4113949087621904 1107 42-43 1.3633625180947249 1107 44-45 1.257946597730033 1107 46-47 1.8742942930787088 1107 48-49 1.7801036165855244 1107 50-51 1.5238108112799473 1107 52-53 1.608853864418787 1107 54-55 1.2766579516515932 1107 56-57 1.8574325041995294 1107 58-59 2.4499632750340155 1107 60-61 2.933550005130826 1107 62-63 2.6199654821126863 1107 64-65 1.5431035663733823 1107 66-67 1.4264054212932997 1107 68-69 2.2059824798382586 1107 70-71 2.1984894509216097 1107 72-73 0.9622441929378063 1107 74-75 1.4648633221288385 1107 76-77 1.7355592408419298 1107 78-79 1.3606062683928677 1107 80-81 1.2327502190354664 1107 82-83 1.2730930866897232 1107 84-85 1.3102919841957466 1107 86-87 1.4265679134562532 1107 88-89 2.6505131223521126 1107 90-91 1.574349747000305 1107 92-93 1.44280477092164 1107 94-95 1.5920432759003482 1107 96-97 1.912176369044559 1107 98-99 2.0613990283522092 1107 100-101 1.610882300115101 1108 1 0.3240727580300984 1108 2 0.011596253802579781 1108 3 -0.04282106196658475 1108 4 0.33834596998344324 1108 5 0.35652416443476653 1108 6 0.34648766814022025 1108 7 0.44110955148182995 1108 8 0.3302022916181926 1108 9 0.4889812118675536 1108 10-11 0.5081852080139342 1108 12-13 0.44606556980081535 1108 14-15 0.1332116303981934 1108 16-17 0.23998254798566876 1108 18-19 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-0.05737742868381446 2316 74-75 -0.544920461654943 2316 76-77 -0.2991434618607727 2316 78-79 0.07384951163610864 2316 80-81 0.42345292173816773 2316 82-83 0.6538498434464834 2316 84-85 0.9596973896011498 2316 86-87 0.5035408864292279 2316 88-89 0.24505366289265496 2316 90-91 0.6420794767300357 2316 92-93 -0.03989793178106282 2316 94-95 -0.8696323997753304 2316 96-97 -0.6032290363608439 2316 98-99 0.5609125418657257 2316 100-101 0.37077419200699424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 1.0 10 1.0 11 0.0 12 2.0 13 11.0 14 55.0 15 248.0 16 704.0 17 1615.0 18 2328.0 19 2813.0 20 3783.0 21 4918.0 22 4865.0 23 3744.0 24 3845.0 25 4043.0 26 4039.0 27 4351.0 28 4157.0 29 2424.0 30 1395.0 31 1147.0 32 1195.0 33 1427.0 34 1853.0 35 2643.0 36 4074.0 37 9035.0 38 8807.0 39 1810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.531870942356875 18.327021444029118 14.068955341333858 19.07215227228015 2 12.487387099155859 23.413235547461422 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17.025880617200468 46-47 16.831007561320465 28.58240609823569 34.061597098420116 20.52498924202373 48-49 16.35212393188664 27.279768857195545 35.44353599311489 20.924571217802914 50-51 12.943382307739595 29.264154423065104 36.345361775373455 21.447101493821847 52-53 11.878650027663367 31.316776295567717 37.65230220692199 19.15227146984693 54-55 11.67148214175939 31.232556709903488 32.96059506977316 24.135366078563962 56-57 15.990655929181779 31.00571709596115 27.858855351324767 25.144771623532304 58-59 11.672711624761787 32.390114956660724 33.44255240671298 22.49462101186451 60-61 15.275096821786438 34.99477469723981 27.45988811704678 22.27024036392697 62-63 11.535624269994466 37.99409848158849 27.801684391713284 22.668592856703757 64-65 11.512264092948914 36.63797872994406 28.409048994897645 23.44070818220938 66-67 13.233540296305405 34.79867215835741 28.90760435236983 23.060183192967358 68-69 13.541525788405975 32.96366877727915 29.367430995266492 24.12737443904838 70-71 13.161000799163952 31.94073891928444 30.453064486383475 24.445195795168132 72-73 14.672035409110467 29.517427921558987 30.57232433761603 25.238212331714514 74-75 15.36177537345546 27.225671605090056 30.678059875822218 26.73449314563226 76-77 14.678182824122457 26.846376098850435 32.952603430257575 25.522837646769535 78-79 15.758283641728655 25.610130939939758 33.65709719063134 24.974488227700252 80-81 14.737198008237536 26.329993237843485 33.277186942890516 25.65562181102846 82-83 14.91116985307678 26.656420974980023 31.26575275096822 27.166656420974984 84-85 15.190262494621013 27.09350218233233 30.15798856580808 27.55824675723858 86-87 16.52302206921989 26.001721276203355 28.540603676154173 28.934652978422577 88-89 15.972213684145817 25.349480543431486 28.252289912091967 30.42601586033073 90-91 16.944734739042232 24.63269195303375 27.451281736029998 30.971291571894017 92-93 16.837769717833652 24.29396938587324 26.758468064179013 32.109792832114096 94-95 16.690231757545952 23.796643511403452 25.81975779184853 33.693366939202065 96-97 17.655990655929184 23.189893649720293 25.397430380524987 33.756685313825535 98-99 18.761910616585727 23.3325136779984 24.488842441753242 33.41673326366263 100-101 18.653830785214147 23.818305884956565 24.11338361949726 33.414479710332024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98.0 1 62.5 2 72.0 3 125.0 4 89.5 5 52.0 6 64.0 7 125.0 8 167.5 9 152.5 10 126.0 11 93.5 12 87.0 13 97.0 14 101.0 15 111.0 16 146.0 17 176.5 18 185.5 19 199.5 20 229.0 21 285.0 22 352.0 23 463.5 24 605.0 25 724.0 26 852.5 27 1040.0 28 1302.5 29 1584.0 30 1924.0 31 2209.5 32 2630.5 33 3038.0 34 3043.0 35 3077.5 36 3398.0 37 3436.5 38 3247.0 39 3180.0 40 2814.5 41 2460.5 42 2445.5 43 2422.5 44 2327.5 45 2271.5 46 2248.0 47 2264.0 48 2278.5 49 2447.5 50 3646.0 51 3761.0 52 2813.0 53 2537.5 54 1781.5 55 1161.5 56 830.0 57 518.0 58 375.0 59 261.5 60 166.0 61 117.5 62 109.0 63 74.0 64 46.5 65 46.5 66 43.0 67 41.0 68 37.5 69 30.0 70 20.5 71 8.5 72 5.5 73 4.0 74 3.0 75 3.0 76 3.0 77 4.5 78 3.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008606381016782443 2 0.08483432716542694 3 0.0 4 0.0 5 0.0 6 0.0073768980143849505 7 0.029507592057539802 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0030737075059937297 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.14741501198746E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 81335.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.72840720477039 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38373491779973 27.449437511526405 2 8.074217183683885 5.446609700620889 3 2.876098129989429 2.910186266674863 4 1.8773010607662306 2.532734984938833 5 1.0060875587795721 1.6966865433085387 6 0.729048955637371 1.4753796028769903 7 0.45201035249517 1.067191246081023 8 0.4301388838260489 1.1606319542632324 9 0.35358874348412495 1.0733386610930105 >10 2.354828126708709 15.341488903915904 >50 0.23329566580395875 5.3912829655130015 >100 0.19319797324390334 13.486199053298087 >500 0.010935734334560566 3.1499354521423744 >1k 0.02187146866912113 11.434191922296675 >5k 0.0036452447781868554 6.384705231450175 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 5193 6.384705231450175 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1983 2.4380647937542266 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1915 2.354459949591197 TruSeq Adapter, Index 10 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1462 1.7975041495051332 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 1393 1.712669822339706 TruSeq Adapter, Index 10 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 1353 1.6634905022438065 RNA PCR Primer, Index 10 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 1.4680027048626052 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 987 1.2134997233663245 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 985 1.2110407573615294 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 590 0.7253949714145203 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 461 0.5667916641052437 TruSeq Adapter, Index 10 (96% over 26bp) CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 455 0.5594147660908588 RNA PCR Primer, Index 10 (96% over 28bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 427 0.524989242023729 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 407 0.5003995819757792 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 380 0.46720354091104693 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.44138439786069955 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC 333 0.4094178397983648 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 326 0.4008114587815823 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.38728714575520995 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.3614680027048626 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 280 0.34425524067129776 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 277 0.34056679166410525 TruSeq Adapter, Index 10 (95% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 252 0.3098297166041679 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 245 0.3012233355873855 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 240 0.29507592057539805 No Hit GACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCACATTT 239 0.29384643757300055 No Hit CTTTTATCCAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAA 230 0.28278109055142314 No Hit GTAATAAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGT 221 0.2717157435298457 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 219 0.26925677752505073 No Hit ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC 214 0.26310936251306327 Illumina Paired End PCR Primer 2 (95% over 24bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 212 0.26065039650826827 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 207 0.2545029814962808 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 202 0.24835556648429336 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 188 0.23114280445072846 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 180 0.22130694043154853 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 175 0.21515952541956107 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 171 0.21024159340997112 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 169 0.20778262740517614 No Hit AAATAATACAACACACCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAA 168 0.2065531444027786 No Hit TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT 167 0.20532366140038114 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.20532366140038114 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.20286469539558616 No Hit CCTTAGACTAAGACAGTCTGGCAAACAGGTAAAAATAATTTACCAGACCA 161 0.19794676338599618 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 160 0.1967172803835987 No Hit TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 160 0.1967172803835987 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 154 0.18934038236921374 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 144 0.17704555234523883 No Hit TTATTAAACTCTTTGTAGCACAAAGGATAAATACTTGAGGTGTTCAATAC 135 0.1659802053236614 No Hit GTACATGGGAAGATGCCAATTGCTGACTGGAAGTGCTGTGCTAAAGTTAT 130 0.15983279031167394 No Hit GTGTATATATTTATGGGATACATGAGATGGTTTGACACAGGCATGCAATG 128 0.15737382430687896 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 128 0.15737382430687896 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.15491485830208396 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.14139054527571157 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.14016106227331407 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 113 0.13893157927091657 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1377020962685191 No Hit GGTTAAGCATTCAACAATTATTCTGTTTTAAAAAGTGCTTTAACAACTAC 112 0.1377020962685191 No Hit GTGCTAAAGTTATCCTGGGAGAATTAGGGTCAAGTCTTATGATGCCAGAG 111 0.1364726132661216 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.1327841642589291 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 108 0.1327841642589291 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 103 0.12663674924694165 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.12417778324214668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 101 0.12417778324214668 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 99 0.12171881723735169 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.12171881723735169 No Hit GTTTTAAAAAGTGCTTTAACAACTACCTACCCTGTTCTTTTCTTAAAAGC 97 0.11925985123255672 No Hit GTACATGGGGATTAACACAGGTGACGATATCAAGGCCAGTTTATTGCAGA 93 0.11434191922296674 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 92 0.11311243622056925 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.11311243622056925 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 91 0.11188295321817175 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 89 0.10942398721337676 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 88 0.10819450421097929 No Hit ATATTAAAGTTTTGTTATTACAAGGGGAAAGGCTCGTGAAAATATATTTT 86 0.1057355382061843 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 86 0.1057355382061843 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 84 0.1032765722013893 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 84 0.1032765722013893 No Hit GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 83 0.10204708919899182 No Hit CAGTAACAGTGAGTATTTTCTTCTCACTTTCCTAAATTCTAACCTAACCA 83 0.10204708919899182 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 83 0.10204708919899182 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.14741501198746E-4 0.0 12-13 0.0 0.0 0.0 0.004917932009589968 0.0 14-15 0.0 0.0 0.0 0.00922112251798119 0.0 16-17 0.0 0.0 0.0 0.015983279031167395 0.0 18-19 0.0 0.0 0.0 0.02151595254195611 0.0 20-21 0.0 0.0 0.0 0.03381078256593103 0.0 22-23 0.0 0.0 0.0 0.06147415011987459 0.0 24-25 0.0 0.0 0.0 0.11434191922296674 0.0 26-27 0.0 0.0 0.0 0.17704555234523883 0.0 28-29 0.0 0.0 0.0 0.28401057355382064 0.0 30-31 0.0 0.0 0.0 0.46843302391344444 0.0 32-33 0.0 0.0 0.0 0.721091780906129 0.0 34-35 0.0 0.0 0.0 1.1231327226901089 0.0 36-37 0.0 0.0 0.0 1.6573430872318191 0.0 38-39 0.0 0.0 0.0 2.300977438986906 0.0 40-41 0.0 0.0 0.0 3.3669392020655318 0.0 42-43 0.0 0.0 0.0 3.997049240794246 0.0 44-45 0.0 0.0 0.0 4.4304419991393615 0.0 46-47 0.0 0.0 0.0 4.901333989057601 0.0 48-49 0.0 0.0 0.0 5.372225978975841 0.0 50-51 0.0 0.0 0.0 5.82836417286531 0.0 52-53 0.0 0.0 0.0 6.360730312903424 0.0 54-55 0.0 0.0 0.0 6.799655744759328 0.0 56-57 0.0 0.0 0.0 7.154976332452204 0.0 58-59 0.0 0.0 0.0 7.426077334480851 0.0 60-61 0.0 0.0 0.0 7.673203417962746 0.0 62-63 0.0 0.0 0.0 7.96520563103215 0.0 64-65 0.0 0.0 0.0 8.236921374561996 0.0 66-67 0.0 0.0 0.0 8.479129526034303 0.0 68-69 0.0 0.0 0.0 8.810475195180427 0.0 70-71 0.0 0.0 0.0 9.24448269502674 0.0 72-73 0.0 0.0 0.0 9.56845146615848 0.0 74-75 0.0 0.0 0.0 9.894264461793817 0.0 76-77 0.0 0.0 0.0 10.256347205999877 0.0 78-79 0.0 0.0 0.0 10.595684514661585 0.0 80-81 0.0 0.0 0.0 10.923956476301715 0.0 82-83 0.0 0.0 0.0 11.321694227577304 0.0 84-85 0.0 0.0 0.0 11.683162230282168 0.0 86-87 0.0 0.0 0.0 12.001598327903118 0.0 88-89 0.0 0.0 0.0 12.385811766152333 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAAAGT 60 1.4700327E-6 47.523693 4 GGATTAA 50 2.8056265E-5 47.49446 9 ATGGGAC 55 4.9080612E-5 43.203354 5 GGGATTA 55 4.925939E-5 43.176785 8 TTATTAA 45 9.491041E-4 42.295338 1 AAGTTTT 45 9.548811E-4 42.243282 7 TAAATAA 60 8.1987484E-5 39.603077 5 GGGGATT 75 6.8198897E-6 38.018955 7 ATGGGCA 50 0.0016030595 38.01895 5 AAAGTAC 50 0.0016030595 38.01895 3 AGTACTC 50 0.0016030595 38.01895 5 CCTTTGT 25 0.0016126614 37.99557 82-83 GTAATAA 65 1.3039939E-4 36.601734 1 ATGGGGA 130 1.9826984E-10 36.556686 5 TGGGGAT 65 1.313491E-4 36.556686 6 ATAATAC 65 1.3182609E-4 36.534203 8 AAGTACT 55 0.0025593336 34.562683 4 GTTTTGT 55 0.002567076 34.541428 9 CTTATAC 1630 0.0 34.154137 1 TATACAC 1660 0.0 33.209328 3 >>END_MODULE