FastQCFastQC Report
Fri 10 Feb 2017
SRR2568654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771187
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.042.6635971
GTATCAA28200.042.4511451
GTACATG88700.036.204381
TACATGG93250.034.0293272
TCAACGC34150.033.2417534
ACATGGG92600.033.188093
CAACGCA35400.032.067965
ATCAACG36700.030.932913
AACGCAG37150.030.6852116
GTATCGC650.005846026429.2296316
TATCAAC40650.028.3969352
CATGGGG53050.028.2929614
CATGGGA41650.026.4575524
GAGTACA58650.026.324091
AGTACAT56600.026.1017532
TACCGTA2251.0186341E-1025.3323467
ACCGTAT2501.4551915E-1124.6990388
ACGCAGA47000.024.0522547
TAGCGCG2002.0565494E-823.7490754
CGCAGAG51100.022.2153788
Produced by FastQC (version 0.11.5)