FastQCFastQC Report
Fri 10 Feb 2017
SRR2568654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771187
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.045.83381
GTATCAA31450.040.826991
GTACATG82900.033.6734621
TCAACGC37350.032.562064
CAACGCA38650.031.4668275
TACATGG86450.031.4060942
ACATGGG84850.030.9064963
ATCAACG40000.030.4048273
AACGCAG40800.029.8086556
TATCAAC44200.027.9700972
CATGGGG45500.026.7295174
CATGGGA41700.024.0385864
ACGCAGA50050.024.01487
GAGTACA57000.023.777941
AGTACAT55300.023.3016682
CGCAGAG53200.022.6776898
GTATAGG6550.022.5073621
CCCTTAT4400.020.5354921
TATAGGG3503.6379788E-1220.3782122
ATGGGGG22900.019.9158235
Produced by FastQC (version 0.11.5)