FastQCFastQC Report
Fri 10 Feb 2017
SRR2568653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753355
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.12849651097467427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.047.8661041
GTATCAA34400.040.8735051
TCAACGC39950.034.0042424
ATCAACG39950.033.7645263
CAACGCA40950.033.289855
AACGCAG42700.032.8154226
TATCAAC44400.031.1328332
ACGCAGA48350.028.4895347
CGCAGAG48250.028.3516948
GTACATG56950.025.7734991
ACATGGG55300.024.7358263
TACATGG58700.024.2768172
GCAGAGT59500.022.3524389
GTATAGG7800.020.7058431
CCTATAC6250.020.5183663
AGAGTAC53150.020.19652210-11
GAGTACT29650.019.8632312-13
CATGGGG36150.019.8404944
CTATACG1456.1487383E-419.654734
GTATTAT11500.019.4137131
Produced by FastQC (version 0.11.5)