##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568653_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1753355 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.694482862854358 33.0 31.0 34.0 29.0 34.0 2 31.847149037131672 34.0 31.0 34.0 30.0 34.0 3 31.8729435852979 34.0 31.0 34.0 30.0 34.0 4 35.24202172406615 37.0 35.0 37.0 32.0 37.0 5 35.28835746326329 37.0 35.0 37.0 32.0 37.0 6 35.40421249547296 37.0 35.0 37.0 33.0 37.0 7 35.384462929640605 37.0 35.0 37.0 33.0 37.0 8 35.40083554100567 37.0 35.0 37.0 33.0 37.0 9 37.15053198011812 39.0 37.0 39.0 33.0 39.0 10-11 37.111676186511005 39.0 37.0 39.0 33.0 39.0 12-13 37.07963105018665 39.0 37.0 39.0 33.0 39.0 14-15 38.410002537991446 40.5 38.0 41.0 33.0 41.0 16-17 38.37854940956053 40.5 38.0 41.0 33.0 41.0 18-19 38.37378597032546 40.5 38.5 41.0 33.0 41.0 20-21 38.3714957895007 40.0 38.0 41.0 33.0 41.0 22-23 38.30328826735031 40.0 38.0 41.0 33.0 41.0 24-25 38.24329100495906 40.0 38.0 41.0 33.0 41.0 26-27 38.14284215119015 40.0 38.0 41.0 33.0 41.0 28-29 38.01569819004138 40.0 38.0 41.0 32.0 41.0 30-31 37.915397338245825 40.0 38.0 41.0 32.0 41.0 32-33 37.79311405847646 40.0 38.0 41.0 32.0 41.0 34-35 37.63798888416778 40.0 38.0 41.0 31.0 41.0 36-37 37.5157287029723 40.0 37.0 41.0 31.0 41.0 38-39 37.41200441439412 40.0 37.0 41.0 31.0 41.0 40-41 37.28327606217794 40.0 37.0 41.0 30.0 41.0 42-43 37.12485435065916 40.0 37.0 41.0 30.0 41.0 44-45 36.93655363574405 40.0 36.5 41.0 30.0 41.0 46-47 36.966391289841475 40.0 36.5 41.0 30.0 41.0 48-49 37.12502374020093 40.0 37.0 41.0 30.0 41.0 50-51 36.04758591386228 39.0 35.5 40.0 28.5 40.5 52-53 36.19946787729809 39.0 35.5 40.0 28.5 40.5 54-55 36.82306749061086 40.0 36.0 41.0 29.0 41.0 56-57 36.81219661734218 40.0 36.0 41.0 29.0 41.0 58-59 36.67446866150894 40.0 36.0 41.0 29.0 41.0 60-61 36.47811595484086 39.0 35.0 41.0 28.0 41.0 62-63 36.23003071254823 39.0 35.0 41.0 28.0 41.0 64-65 36.0743645753427 39.0 35.0 41.0 28.0 41.0 66-67 35.96083964741881 39.0 35.0 41.0 28.0 41.0 68-69 35.68556025448355 38.0 35.0 40.5 28.0 41.0 70-71 35.33995197777974 37.0 35.0 40.0 28.0 41.0 72-73 34.98820318760319 37.0 34.0 39.5 27.5 41.0 74-75 34.63028679303393 36.0 34.0 39.0 27.0 41.0 76-77 34.26624100652749 36.0 34.0 39.0 26.0 40.5 78-79 33.89847977163781 35.0 34.0 37.5 26.0 39.5 80-81 33.52515890963325 35.0 34.0 37.0 26.0 39.0 82-83 33.19819004137781 35.0 33.0 36.5 26.0 39.0 84-85 32.882022180334275 35.0 33.0 36.0 25.5 37.0 86-87 32.628623695714786 35.0 33.0 36.0 25.0 37.0 88-89 32.40273019439874 35.0 33.0 35.5 25.0 37.0 90-91 32.21661443347183 35.0 33.0 35.0 25.0 36.0 92-93 32.054101993036205 35.0 33.0 35.0 24.0 36.0 94-95 31.905565330466448 35.0 32.0 35.0 24.0 36.0 96-97 31.799879374114198 35.0 32.0 35.0 24.0 36.0 98-99 31.657975994593222 35.0 32.0 35.0 23.0 35.0 100-101 30.367684809978584 34.0 29.5 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19867057882612116 1101 2 0.17327876574615786 1101 3 0.37386450279543837 1101 4 0.23031952930426058 1101 5 0.15755364132862582 1101 6 0.04579696675988032 1101 7 0.11951606736048603 1101 8 0.2104654819968843 1101 9 0.35039726684911443 1101 10-11 0.22520225547620498 1101 12-13 0.19958873155967893 1101 14-15 0.3245215493411493 1101 16-17 0.36595643591207505 1101 18-19 0.38604456492683425 1101 20-21 0.22977264372235595 1101 22-23 0.16607394190000235 1101 24-25 0.19825075205866938 1101 26-27 0.31269825825733477 1101 28-29 0.3954271291879863 1101 30-31 0.5026123414955634 1101 32-33 0.35045010275170796 1101 34-35 0.12218614346912204 1101 36-37 0.13933930793926663 1101 38-39 0.3055626816480128 1101 40-41 0.2898969615326834 1101 42-43 0.2789014447163183 1101 44-45 0.3429547165699276 1101 46-47 0.24405314444323523 1101 48-49 0.27730058969861915 1101 50-51 0.32388647337927523 1101 52-53 0.1874616024451612 1101 54-55 0.3156207874302268 1101 56-57 0.27870958870885687 1101 58-59 0.21485804424007426 1101 60-61 0.2968943084948208 1101 62-63 0.33753524279294567 1101 64-65 0.2663152965464448 1101 66-67 0.30306371139218413 1101 68-69 0.3158606926262948 1101 70-71 0.2076779528761037 1101 72-73 0.15393292705254424 1101 74-75 0.20204158480883194 1101 76-77 0.23750099762983723 1101 78-79 0.1626349529354627 1101 80-81 0.010226896034389199 1101 82-83 0.1715402911610937 1101 84-85 0.03126076844365144 1101 86-87 0.03135800660176358 1101 88-89 0.05434761238066699 1101 90-91 0.1345923343934956 1101 92-93 0.07310016483408077 1101 94-95 -0.1517449360774883 1101 96-97 -0.13092419230881447 1101 98-99 0.007633565064995906 1101 100-101 -0.20206690479682266 1102 1 0.18321473245004327 1102 2 0.17414104322203272 1102 3 0.23907214954548195 1102 4 0.2083271478983022 1102 5 0.16843900522619748 1102 6 0.23355704469808103 1102 7 0.1937610410278836 1102 8 0.3406734994176617 1102 9 0.3321681294336116 1102 10-11 0.4369432322647242 1102 12-13 0.3068209668839259 1102 14-15 0.37681645048439094 1102 16-17 0.35467288178628564 1102 18-19 0.3644750851313816 1102 20-21 0.28219054015470135 1102 22-23 0.28746654810465344 1102 24-25 0.32938713118220164 1102 26-27 0.38239296784991694 1102 28-29 0.38756310618992273 1102 30-31 0.33566898223025277 1102 32-33 0.32604667670575793 1102 34-35 0.24752845227603615 1102 36-37 0.060498204373857334 1102 38-39 0.14904565363354294 1102 40-41 0.1439558435148669 1102 42-43 0.10330348542719747 1102 44-45 0.32714242722509823 1102 46-47 0.5511914765147949 1102 48-49 0.3907790013108752 1102 50-51 0.36495454425151763 1102 52-53 0.3049149896642902 1102 54-55 0.3636688877614276 1102 56-57 0.37849134227347747 1102 58-59 0.30026769530786623 1102 60-61 0.11474128063910882 1102 62-63 0.3298271635937198 1102 64-65 0.3452554328484396 1102 66-67 0.3325631112051255 1102 68-69 0.199067211638706 1102 70-71 0.23597154955788824 1102 72-73 0.16869273616085678 1102 74-75 0.2130531265220199 1102 76-77 0.3207963278525341 1102 78-79 0.41428967951150497 1102 80-81 0.3536398524367712 1102 82-83 0.20648986122048285 1102 84-85 0.2735946237113325 1102 86-87 0.21377612019474412 1102 88-89 0.17649410295252466 1102 90-91 0.17375950090402625 1102 92-93 0.34347432511510334 1102 94-95 0.24907181254859623 1102 96-97 0.165727153300832 1102 98-99 0.16486329180952097 1102 100-101 0.0030437460335903666 1103 1 0.1999244382866756 1103 2 0.22289981659129054 1103 3 0.28549327702779337 1103 4 0.25988850534508146 1103 5 0.21400150846493204 1103 6 0.17578523578624328 1103 7 0.27502590321617504 1103 8 0.2947561749282315 1103 9 0.29559850961774714 1103 10-11 0.3251846143546686 1103 12-13 0.2689590923913201 1103 14-15 0.3059745525001816 1103 16-17 0.3005860319080682 1103 18-19 0.2741464472419466 1103 20-21 0.26147344136900585 1103 22-23 0.24199328996751746 1103 24-25 0.3540159003483012 1103 26-27 0.2365686309986046 1103 28-29 0.10468060456336303 1103 30-31 0.3867751176870442 1103 32-33 0.2897573872695034 1103 34-35 0.34000616978938325 1103 36-37 0.3282561802949573 1103 38-39 0.2043117623743811 1103 40-41 0.23921167467464954 1103 42-43 0.24872693903989074 1103 44-45 0.09713368984176185 1103 46-47 0.15105242688876075 1103 48-49 0.1900243044275456 1103 50-51 0.3049008676412157 1103 52-53 0.2930889341250449 1103 54-55 0.2713149618406234 1103 56-57 0.16797582226242724 1103 58-59 0.3044546580379688 1103 60-61 0.29411601794183184 1103 62-63 0.4502450753734948 1103 64-65 0.26854890816785115 1103 66-67 0.07839342172049868 1103 68-69 0.0950070633191018 1103 70-71 0.2755776619836894 1103 72-73 0.20060541702613932 1103 74-75 0.15500566540121952 1103 76-77 0.16745309119179552 1103 78-79 0.23303251831182337 1103 80-81 0.25129803218217006 1103 82-83 0.210368064687799 1103 84-85 0.09117079872385858 1103 86-87 0.02803187362492565 1103 88-89 0.05633744901047777 1103 90-91 0.04375097943626116 1103 92-93 0.004141057995873609 1103 94-95 -0.08674131108753613 1103 96-97 -0.05380104239351979 1103 98-99 0.062119508738454954 1103 100-101 -0.19107730487066377 1104 1 0.19057235802310402 1104 2 0.17908776601817422 1104 3 0.20276078915507512 1104 4 0.0875176008898606 1104 5 0.18360604039872896 1104 6 0.11044277456871043 1104 7 0.25194957445782507 1104 8 0.23284701779506634 1104 9 0.28889263278347244 1104 10-11 0.05505456683890486 1104 12-13 0.07383573626587037 1104 14-15 0.24831408330265248 1104 16-17 0.1400713053306859 1104 18-19 0.09497550360414664 1104 20-21 0.08991792076504623 1104 22-23 0.07897446577586464 1104 24-25 0.07794017312524204 1104 26-27 0.11919129728999422 1104 28-29 0.2294012217716883 1104 30-31 0.23476707974065647 1104 32-33 0.21355245412821233 1104 34-35 0.09238644219104941 1104 36-37 0.05910121321383599 1104 38-39 -0.03254862349119492 1104 40-41 0.03808952161978851 1104 42-43 -0.09510152958369389 1104 44-45 0.2036492414606741 1104 46-47 0.12135645055282396 1104 48-49 0.2582730365125556 1104 50-51 0.014983275217254288 1104 52-53 0.1229272232262204 1104 54-55 0.17053538037674087 1104 56-57 0.2292006640144919 1104 58-59 0.09202336550968937 1104 60-61 0.0819182342406819 1104 62-63 0.019065334971841708 1104 64-65 0.05129705527358652 1104 66-67 0.11428376294001907 1104 68-69 0.09767292701893382 1104 70-71 0.22287709963096347 1104 72-73 0.21573865157523642 1104 74-75 0.12816353254469703 1104 76-77 0.04685906129162731 1104 78-79 0.1293149336139905 1104 80-81 0.17824667541654549 1104 82-83 0.11103851440442014 1104 84-85 0.22573362329077895 1104 86-87 0.22890672117159738 1104 88-89 0.1121377806826942 1104 90-91 0.19711902521916613 1104 92-93 0.209143527601789 1104 94-95 0.22989493389736637 1104 96-97 -0.0014402233121622032 1104 98-99 -0.015800351859876827 1104 100-101 0.014689155949522359 1105 1 0.23428623826210426 1105 2 0.28328997821235546 1105 3 0.44630678440326577 1105 4 0.11319124795779345 1105 5 0.2664763801854946 1105 6 0.22256352419683623 1105 7 0.3225231549336556 1105 8 0.3434153437971972 1105 9 0.3621648259112007 1105 10-11 0.35748559617663034 1105 12-13 0.4411608446773627 1105 14-15 0.5689174669711079 1105 16-17 0.4886063550241886 1105 18-19 0.4775900294904005 1105 20-21 0.549529279540053 1105 22-23 0.5484260035067052 1105 24-25 0.6275345839446018 1105 26-27 0.6073446231364699 1105 28-29 0.6933942193502176 1105 30-31 0.6257432045359295 1105 32-33 0.4277335724958675 1105 34-35 0.5728047954906188 1105 36-37 0.6883546223573802 1105 38-39 0.6112967704538619 1105 40-41 0.5848190293621585 1105 42-43 0.5448988728543185 1105 44-45 0.6691219580615382 1105 46-47 0.5858291466521379 1105 48-49 0.719919554702372 1105 50-51 0.611965331797002 1105 52-53 0.5541291157479336 1105 54-55 0.6016843105370455 1105 56-57 0.6424754615995596 1105 58-59 0.6930533410672766 1105 60-61 0.5990545005118904 1105 62-63 0.6156111639296142 1105 64-65 0.6108724983540341 1105 66-67 0.5851276982371019 1105 68-69 0.4618628154980513 1105 70-71 0.47513132200302266 1105 72-73 0.5091341881579865 1105 74-75 0.4250291478610322 1105 76-77 0.5613331621274043 1105 78-79 0.46395144476494465 1105 80-81 0.5141744051422208 1105 82-83 0.5432730780590944 1105 84-85 0.49991502260572673 1105 86-87 0.5611234788424042 1105 88-89 0.47705205758890656 1105 90-91 0.3436421505983489 1105 92-93 0.44283093045667243 1105 94-95 0.4968054931874981 1105 96-97 0.4357938733625666 1105 98-99 0.4714220513328975 1105 100-101 0.33629369909505513 1106 1 0.14436509198993264 1106 2 0.15338327070228175 1106 3 0.22233774957014063 1106 4 0.1647761013591449 1106 5 0.21384693905908847 1106 6 0.22952386588355012 1106 7 0.18019826899144675 1106 8 0.12557856771832832 1106 9 0.23332723056287108 1106 10-11 0.16609440152234356 1106 12-13 0.25146106976570337 1106 14-15 0.21860524023656325 1106 16-17 0.293016308573371 1106 18-19 0.3735175168622362 1106 20-21 0.37126019128963605 1106 22-23 0.3584355757009021 1106 24-25 0.3390519108423149 1106 26-27 0.16872449823044633 1106 28-29 0.2597485315508976 1106 30-31 0.2325938760073356 1106 32-33 0.2839140370959896 1106 34-35 0.354040305955273 1106 36-37 0.27363643495849743 1106 38-39 0.21416068818663803 1106 40-41 0.30897295545624104 1106 42-43 0.340223672194611 1106 44-45 0.43062449365837807 1106 46-47 0.31808207636433394 1106 48-49 0.2503794798568535 1106 50-51 0.34633138660451834 1106 52-53 0.3972725430839503 1106 54-55 0.2559690745008325 1106 56-57 0.31917881784536206 1106 58-59 0.2989308905549066 1106 60-61 0.3234849013101595 1106 62-63 0.3545634799944253 1106 64-65 0.28494196841928243 1106 66-67 0.3611186195971925 1106 68-69 0.33606605870151895 1106 70-71 0.367717738070823 1106 72-73 0.33458610838306413 1106 74-75 0.2656084303833097 1106 76-77 0.35875709997022653 1106 78-79 0.32700967267513903 1106 80-81 0.32567307047638394 1106 82-83 0.2028300211187215 1106 84-85 0.1645895258517598 1106 86-87 0.23137767462864645 1106 88-89 0.22547502135174824 1106 90-91 0.20082830869973378 1106 92-93 0.08739443763504795 1106 94-95 0.17742720298524617 1106 96-97 0.2013578855910616 1106 98-99 0.11671975883453811 1106 100-101 0.04827411633236167 1107 1 0.21952359149998912 1107 2 0.11221946135394845 1107 3 0.286074814006529 1107 4 0.2755952994975459 1107 5 0.2786312180208057 1107 6 0.20167008550675547 1107 7 0.27355369121764994 1107 8 0.31537210765132073 1107 9 0.32077141509167006 1107 10-11 0.262081067012204 1107 12-13 0.2780864427009888 1107 14-15 0.3142868573857527 1107 16-17 0.3513349800443635 1107 18-19 0.20070779293903485 1107 20-21 0.20802598954026053 1107 22-23 0.2145812850848543 1107 24-25 0.15940920072456777 1107 26-27 0.3215931766914579 1107 28-29 0.3059619056174725 1107 30-31 0.308146651302998 1107 32-33 0.14630260747905055 1107 34-35 0.13465063456352766 1107 36-37 0.33998042254266636 1107 38-39 0.19034147007675273 1107 40-41 0.14389074702221905 1107 42-43 0.26525490180033984 1107 44-45 0.3854812046896612 1107 46-47 0.40773761977235523 1107 48-49 0.38408455126135976 1107 50-51 0.274367197411415 1107 52-53 0.24198699271167357 1107 54-55 0.2640404538798222 1107 56-57 0.42138660118437343 1107 58-59 0.5079371609572263 1107 60-61 0.5214989625246957 1107 62-63 0.5258215483136581 1107 64-65 0.4889062439407468 1107 66-67 0.4566140509587342 1107 68-69 0.47682911763737934 1107 70-71 0.586254111622651 1107 72-73 0.5051293907805174 1107 74-75 0.516427422364437 1107 76-77 0.5484369312820334 1107 78-79 0.3328571304660457 1107 80-81 0.4332330509687594 1107 82-83 0.43339808748590514 1107 84-85 0.45722317213613195 1107 86-87 0.5262091507211721 1107 88-89 0.37221744652149624 1107 90-91 0.41646752831714196 1107 92-93 0.36406329687022065 1107 94-95 0.28576059537404674 1107 96-97 0.4107511840623381 1107 98-99 0.4200263926534795 1107 100-101 0.4991584343995328 1108 1 -0.012632214198461611 1108 2 -0.029592577141901444 1108 3 0.08115959862453792 1108 4 0.22806685178686337 1108 5 0.151924902942973 1108 6 0.005245159281713541 1108 7 0.06040167173317457 1108 8 0.01976122473191566 1108 9 0.04991766983538781 1108 10-11 -0.0037150965440702066 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-0.9250304003523624 2316 62-63 -0.7925257448345775 2316 64-65 -0.9561431094043868 2316 66-67 -0.8604719599478372 2316 68-69 -0.6817290891779564 2316 70-71 -0.6412191930623692 2316 72-73 -0.650711649499776 2316 74-75 -0.46516920561650466 2316 76-77 -0.6914134823919582 2316 78-79 -0.6503151463853456 2316 80-81 -0.4491491489378987 2316 82-83 -0.38920263292326496 2316 84-85 -0.605604857255301 2316 86-87 -0.46334695966017136 2316 88-89 -0.36160768882601957 2316 90-91 -0.3730958934885322 2316 92-93 -0.2349518518101803 2316 94-95 -0.2718361960676994 2316 96-97 -0.19792809206234097 2316 98-99 -0.3086481273527433 2316 100-101 -0.11440521782678204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 16.0 7 206.0 8 447.0 9 458.0 10 724.0 11 1383.0 12 2580.0 13 3779.0 14 4616.0 15 5308.0 16 5929.0 17 6745.0 18 7863.0 19 8932.0 20 10163.0 21 11927.0 22 13225.0 23 13710.0 24 13538.0 25 13843.0 26 14730.0 27 16626.0 28 18808.0 29 21729.0 30 25785.0 31 30957.0 32 38934.0 33 50685.0 34 69788.0 35 103197.0 36 175784.0 37 348004.0 38 527910.0 39 184910.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.55915711063137 20.933805978023734 14.278299505776399 23.228737405568502 2 13.942876698655335 21.601505796460806 42.172681772162726 22.28293573272113 3 16.86494526734959 28.558506303109322 30.98809643988249 23.5884519896586 4 11.231490400930335 18.64126154149749 42.12206710936284 28.00518094820934 5 12.16490447189481 39.13310055403906 36.42598610852869 12.276008865537449 6 27.163503355712354 40.54340467204592 20.101364357868885 12.19172761437284 7 24.810569008776138 33.55944325967888 25.166131763549053 16.46385596799593 8 23.98938451640575 37.01513955493906 22.26947312706184 16.72600280159335 9 25.708964907527555 16.27167077813743 21.924681658087238 36.09468265624777 10-11 23.207953049198355 27.610517559067087 30.51958959400584 18.661939797728717 12-13 24.160522992721127 24.96383949297722 30.339577960113935 20.53605955418772 14-15 21.667032631726034 26.260369406081484 27.68466739479455 24.38793056739793 16-17 21.299850857356326 29.162405787761177 28.1160974246516 21.421645930230902 18-19 21.232683540426958 28.601020843771664 29.42392962970673 20.742365986094647 20-21 22.3704127446194 27.71981525503383 29.09547856200979 20.814293438336975 22-23 21.959943570926878 27.789065683635027 28.699493512852385 21.55149723258571 24-25 21.545897053694638 27.900856170096755 29.003927665703834 21.549319110504772 26-27 21.655627911486533 28.121120691498998 28.77697246751469 21.446278929499783 28-29 21.722731624240684 27.89429241916069 28.826558475020224 21.5564174815784 30-31 21.90151445580608 27.87494773755669 28.788292756382578 21.435245050254654 32-33 21.738012077465513 27.938321428489953 28.813447187894248 21.51021930615029 34-35 21.992694006633 28.088122485178413 28.61094872401767 21.308234784170917 36-37 22.01323234976156 27.85962564900595 28.660034237229265 21.46710776400323 38-39 21.863731415256535 28.08577962173629 28.557532750811514 21.492956212195658 40-41 22.01850167250785 27.84738401521654 28.61591063988753 21.51820367238808 42-43 21.816005721625142 27.87085050999573 28.538886746954965 21.774257021424155 44-45 22.067447823169545 28.027955779268204 28.305369094023664 21.599227303538587 46-47 21.94920868311193 27.96339954423387 28.261150400256884 21.826241372397316 48-49 21.82720474165465 28.042413443813764 28.374784408023217 21.755597406508368 50-51 21.971656264838114 28.090383976478606 28.17910251968077 21.75885723900251 52-53 21.988730177288684 28.117209578208634 28.10414890310291 21.789911341399772 54-55 21.894710426582183 28.05606964932943 28.289222661697146 21.759997262391245 56-57 21.93702929526536 28.142760593262633 28.10352153442971 21.8166885770423 58-59 22.034385506642977 28.049510795018694 28.13277972800716 21.783323970331164 60-61 22.116228601737813 28.113530916443047 28.191182048130585 21.57905843368856 62-63 21.96974514618826 28.171371358126223 28.13264523650168 21.726238259183837 64-65 22.023338136627824 27.957540891929867 28.170937207071994 21.848183764370315 66-67 22.00136313989865 28.003427812046393 28.078058295618845 21.91715075243611 68-69 21.976667483260073 28.084519984246114 28.09980615650588 21.839006375987932 70-71 22.05303123631187 27.916313056650605 28.091093362169662 21.939562344867863 72-73 21.951173607170254 28.075888796051 28.007819295008712 21.965118301770033 74-75 21.889605926922957 28.003655848359287 28.045661032705873 22.061077192011886 76-77 21.820566856112993 27.9588275049833 28.11498527109456 22.10562036780914 78-79 21.84392208081079 27.918618876382705 28.104605171228876 22.13285387157763 80-81 21.989881350145843 28.07410532031981 27.884754972550045 22.051258356984302 82-83 21.965696104298356 28.05003295544612 27.935551639373017 22.04871930088251 84-85 22.042649778496788 28.036413690187572 27.862856256224966 22.05808027509067 86-87 21.834959254057768 27.966159704927048 28.12964545382982 22.069235587185364 88-89 21.97425088558928 27.838249470928144 28.15304009537554 22.034459548107037 90-91 21.971703403109856 28.027440956537465 28.065288781471164 21.93556685888152 92-93 22.03737045300345 27.852301237211428 28.081485235513938 22.028843074271187 94-95 21.914252928446558 27.80417636825563 28.113086940784154 22.16848376251366 96-97 21.808662946163746 27.708810564646402 28.31057833590392 22.171948153285935 98-99 21.847733578540986 27.776411921472704 28.117213668349045 22.258640831637262 100-101 21.99548334948596 27.65726848173118 28.169398922689293 22.177849246093565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 8.5 2 18.5 3 33.0 4 36.0 5 37.0 6 58.0 7 109.0 8 196.0 9 268.5 10 288.5 11 327.0 12 393.5 13 440.5 14 524.5 15 625.5 16 701.0 17 771.5 18 852.5 19 948.5 20 1122.5 21 1412.0 22 1749.5 23 2231.5 24 3104.0 25 4399.5 26 6388.0 27 9245.5 28 12815.5 29 17845.5 30 24201.0 31 30503.5 32 37210.0 33 44269.5 34 51004.0 35 57112.5 36 62760.0 37 66353.5 38 67204.0 39 67901.0 40 68022.0 41 67707.0 42 67636.0 43 68073.5 44 69187.0 45 69569.5 46 68403.5 47 67033.5 48 65845.5 49 63302.0 50 59927.5 51 56675.5 52 53170.0 53 49434.0 54 45740.0 55 41540.0 56 36593.5 57 31920.5 58 27711.5 59 23425.5 60 19370.0 61 15360.5 62 11758.5 63 8716.0 64 6232.5 65 4385.5 66 3023.5 67 2117.5 68 1458.0 69 1095.5 70 893.0 71 717.5 72 539.0 73 371.5 74 257.5 75 182.5 76 123.0 77 87.0 78 56.5 79 35.5 80 33.5 81 29.0 82 22.0 83 17.0 84 10.5 85 12.0 86 11.0 87 7.5 88 6.0 89 8.0 90 6.5 91 3.0 92 3.0 93 3.5 94 4.0 95 2.5 96 1.5 97 1.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005703351574552786 2 0.007414357046918621 3 7.9846922043739E-4 4 4.562681259642229E-4 5 0.002794642271530865 6 0.004334547196660117 7 0.0044486142281511725 8 0.0038212455549503665 9 0.007414357046918621 10-11 0.006587371068608468 12-13 0.0034790444604771995 14-15 0.0 16-17 0.0 18-19 8.55502736182918E-5 20-21 0.0010836367991650293 22-23 1.996173051093475E-4 24-25 0.0013402876200199047 26-27 0.003564594734095491 28-29 0.0026520584821670455 30-31 0.010494166897177125 32-33 3.99234610218695E-4 34-35 0.0 36-37 2.851675787276393E-5 38-39 8.55502736182918E-5 40-41 0.0 42-43 3.99234610218695E-4 44-45 8.269859783101539E-4 46-47 0.002480957934930462 48-49 0.002452441177057698 50-51 0.003279427155367852 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.410530098012096E-4 64-65 0.0025379914506759894 66-67 0.002851675787276393 68-69 0.007785074899264553 70-71 6.273686732008065E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014572063272982367 82-83 0.013146225379344172 84-85 0.018992160743260777 86-87 0.016938954176421772 88-89 0.01625455198747544 90-91 0.016083451440238858 92-93 0.010180482560576723 94-95 0.023982593370994464 96-97 0.01066526744441371 98-99 0.016140484955984382 100-101 0.01631158550322097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1753355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.16972021159318 #Duplication Level Percentage of deduplicated Percentage of total 1 70.14960158385206 40.104330955035564 2 16.367364339239806 18.714352797510948 3 6.056247258023534 10.387018838203014 4 2.8648073662857594 6.55120942362672 5 1.46968032800149 4.2010606576163845 6 0.8960094469955943 3.073476563500947 7 0.5325262901511931 2.1311065309282986 8 0.3861504495750251 1.7660890529428084 9 0.26255056461930826 1.3508948088613504 >10 0.9670925612405245 8.922481185319233 >50 0.035062743026508886 1.3625452220965144 >100 0.012506979346774258 1.1241322585159574 >500 1.0002241061973612E-4 0.029563369199200305 >1k 3.000672318592078E-4 0.2817383366430553 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2253 0.12849651097467427 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.703351574552786E-5 2 0.0 0.0 0.0 0.0 5.703351574552786E-5 3 0.0 0.0 0.0 0.0 5.703351574552786E-5 4 0.0 0.0 0.0 0.0 5.703351574552786E-5 5 0.0 0.0 0.0 0.0 5.703351574552786E-5 6 0.0 0.0 0.0 0.0 5.703351574552786E-5 7 0.0 0.0 0.0 5.703351574552786E-5 5.703351574552786E-5 8 0.0 0.0 0.0 5.703351574552786E-5 5.703351574552786E-5 9 0.0 0.0 0.0 1.711005472365836E-4 5.703351574552786E-5 10-11 0.0 0.0 0.0 2.2813406298211145E-4 5.703351574552786E-5 12-13 0.0 0.0 0.0 4.27751368091459E-4 1.4258378936381964E-4 14-15 0.0 0.0 0.0 5.133016417097507E-4 2.851675787276393E-4 16-17 0.0 0.0 0.0 5.133016417097507E-4 4.562681259642229E-4 18-19 0.0 0.0 0.0 5.703351574552786E-4 4.562681259642229E-4 20-21 0.0 0.0 0.0 6.273686732008064E-4 6.273686732008064E-4 22-23 0.0 0.0 0.0 6.844021889463342E-4 6.273686732008064E-4 24-25 0.0 0.0 0.0 0.0013973211357654327 6.273686732008064E-4 26-27 0.0 0.0 0.0 0.0018250725038568916 6.558854310735704E-4 28-29 0.0 0.0 0.0 0.002195790356202823 6.844021889463344E-4 30-31 0.0 0.0 0.0 0.0029087093030219207 6.844021889463344E-4 32-33 0.0 0.0 0.0 0.004619714775387756 6.844021889463344E-4 34-35 0.0 2.851675787276393E-5 0.0 0.007357323531173094 7.9846922043739E-4 36-37 0.0 5.703351574552786E-5 0.0 0.013146225379344172 7.9846922043739E-4 38-39 0.0 5.703351574552786E-5 0.0 0.02358335876077577 8.269859783101539E-4 40-41 0.0 5.703351574552786E-5 0.0 0.037699153907793916 8.555027361829179E-4 42-43 0.0 5.703351574552786E-5 0.0 0.05440997402123358 9.125362519284458E-4 44-45 0.0 1.1406703149105573E-4 0.0 0.07237553148107485 9.980865255467377E-4 46-47 0.0 1.711005472365836E-4 0.0 0.09359199933841122 0.0010266032834195015 48-49 0.0 1.711005472365836E-4 0.0 0.11823047814047925 0.0010551200412922653 50-51 0.0 1.711005472365836E-4 0.0 0.14737460468644398 0.001197703830656085 52-53 0.0 1.711005472365836E-4 0.0 0.1790852394409575 0.0012832541042743768 54-55 0.0 2.5665082085487537E-4 0.0 0.21096697474270754 0.0013117708621471409 56-57 0.0 2.851675787276393E-4 0.0 0.24818134376666445 0.0013117708621471409 58-59 0.0 2.851675787276393E-4 0.0 0.2925249022588124 0.0013117708621471409 60-61 0.0 2.851675787276393E-4 0.0 0.34422578428213335 0.0013688043778926687 62-63 0.0 2.851675787276393E-4 0.0 0.3998049453761503 0.0013688043778926687 64-65 0.0 2.851675787276393E-4 0.0 0.4542149193973839 0.0013688043778926687 66-67 0.0 2.851675787276393E-4 0.0 0.5163814515600093 0.0013688043778926687 68-69 0.0 2.851675787276393E-4 0.0 0.5903254047240861 0.0013688043778926687 70-71 0.0 2.851675787276393E-4 0.0 0.6813223790960758 0.0013688043778926687 72-73 0.0 2.851675787276393E-4 0.0 0.7744581103085229 0.0014543546515109603 74-75 0.0 2.851675787276393E-4 0.0 0.8675082912473515 0.0015399049251292522 76-77 0.0 2.851675787276393E-4 0.0 0.9715944574829398 0.00159693844087478 78-79 0.0 2.851675787276393E-4 0.0 1.0900530696864013 0.00159693844087478 80-81 0.0 2.851675787276393E-4 0.0 1.2286730297059067 0.0016824887144930717 82-83 0.0 2.851675787276393E-4 0.0 1.3864847677737822 0.0018535892617296554 84-85 0.0 2.851675787276393E-4 0.0 1.5403041597394709 0.0018821060196024194 86-87 0.0 3.422010944731672E-4 0.0 1.7062146570432115 0.0018821060196024194 88-89 0.0 3.422010944731672E-4 0.0 1.8855280305471511 0.0019106227774751835 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1955 0.0 47.866104 1 GTATCAA 3440 0.0 40.873505 1 TCAACGC 3995 0.0 34.004242 4 ATCAACG 3995 0.0 33.764526 3 CAACGCA 4095 0.0 33.28985 5 AACGCAG 4270 0.0 32.815422 6 TATCAAC 4440 0.0 31.132833 2 ACGCAGA 4835 0.0 28.489534 7 CGCAGAG 4825 0.0 28.351694 8 GTACATG 5695 0.0 25.773499 1 ACATGGG 5530 0.0 24.735826 3 TACATGG 5870 0.0 24.276817 2 GCAGAGT 5950 0.0 22.352438 9 GTATAGG 780 0.0 20.705843 1 CCTATAC 625 0.0 20.518366 3 AGAGTAC 5315 0.0 20.196522 10-11 GAGTACT 2965 0.0 19.86323 12-13 CATGGGG 3615 0.0 19.840494 4 CTATACG 145 6.1487383E-4 19.65473 4 GTATTAT 1150 0.0 19.413713 1 >>END_MODULE