FastQCFastQC Report
Fri 10 Feb 2017
SRR2568653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753355
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.13722263888374003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.10984655132588665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.044.9401131
GTATCAA37500.041.4929431
TCAACGC42150.036.1884544
CAACGCA43450.035.5431755
ATCAACG43200.035.198883
AACGCAG44700.034.7618456
TATCAAC47050.032.6616862
ACGCAGA50200.030.4791167
CGCAGAG50550.030.1611798
GCAGAGT62250.024.1108289
CGTATAC1652.4228866E-623.0392673
GTACATG54550.022.9413381
ACATGGG49400.022.701143
TACATGG52350.022.6296772
CTACGGT1302.93463E-421.931614
GTATAGC9850.021.255641
AGAGTAC55900.020.60460710-11
CATGGGG28850.019.270934
GAGTACT33000.019.14265312-13
CAGAGTA61000.019.11551910-11
Produced by FastQC (version 0.11.5)