FastQCFastQC Report
Fri 10 Feb 2017
SRR2568651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976109
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.031.31781
TCAACGC35600.030.8199254
GTATCAA37950.030.5415761
GTACATG80850.029.729251
CAACGCA37200.029.3666615
ATCAACG37500.029.2588793
ACATGGG78050.028.602363
AACGCAG39600.027.8272156
TACATGG85900.026.8754982
CATGGGG48650.025.0911124
TATCAAC45600.024.2719422
ACGCAGA46700.023.4948147
CGCAGAG48000.022.7595428
GAGTACA55900.021.0743061
GTGTAGC17800.018.9474931
GTATTAC10050.018.9063361
TAATACT28150.018.5602554
AGTACAT55150.018.5185242
AACGTCG1303.2874232E-718.26837722-23
GTATAGG13150.017.7004281
Produced by FastQC (version 0.11.5)