FastQCFastQC Report
Fri 10 Feb 2017
SRR2568651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976109
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.035.5535281
GTATCAA39700.033.187571
TCAACGC38900.031.7561594
ATCAACG40650.030.5059223
CAACGCA40650.030.3890425
AACGCAG43100.028.8820676
TATCAAC49850.024.996882
GTACATG73450.024.8023681
ACGCAGA50300.024.7461917
CGCAGAG51500.023.7949818
TACATGG75750.022.5403922
ACATGGG72050.022.222873
GCACCGT4050.019.9433526
GAGTACA56650.019.731231
GTACCGT3455.2750693E-1119.2802736
CATGGGG43050.019.0931324
TCTATAC12550.018.550543
GCAGAGT66650.018.0298989
GTATAGA12400.018.0286161
AGTACAT54850.017.6022852
Produced by FastQC (version 0.11.5)