FastQCFastQC Report
Fri 10 Feb 2017
SRR2568650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120636
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57600.044.5340771
GGTATCA31450.042.2920271
TCAACGC68400.036.3894464
CAACGCA70550.035.145835
ATCAACG71650.034.60573
AACGCAG74300.033.5637746
TATCAAC74700.033.322062
ACGCAGA86350.028.6049547
GTACATG114000.028.0017761
CGCAGAG89300.027.5536168
TACATGG112550.027.1807562
ACATGGG113800.026.4213353
CGTATAC2700.026.3888723
GCAGAGT108550.022.0546939
CATGGGG78250.021.3070374
AGAGTAC93400.019.52889410-11
GTATTAA18950.018.0486371
GTATAAA21500.017.8964841
GTACCTG27200.017.6389871
ATGGGGG41500.017.5123185
Produced by FastQC (version 0.11.5)