FastQCFastQC Report
Fri 10 Feb 2017
SRR2568650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120636
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33150.10622834576028733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.045.557951
GTATCAA57650.041.5051081
TCAACGC70550.033.202774
CAACGCA72550.032.2219735
ATCAACG73850.031.8477783
AACGCAG75750.030.9235086
TATCAAC78250.030.391762
ACGCAGA87550.026.645817
GTACATG107150.026.415431
TACATGG107850.025.7991812
CGCAGAG93500.024.993378
ACATGGG107950.024.868543
CATGGGG70100.021.215384
GCAGAGT110400.020.6941389
GGACCGT5150.019.374776
AGAGTAC95400.019.19319710-11
CAGAGTA103750.017.4653710-11
ATGGGGG37150.016.6268125
GAGTACA78100.015.9581741
TACCTGG24500.015.7249452
Produced by FastQC (version 0.11.5)