FastQCFastQC Report
Fri 10 Feb 2017
SRR2568649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749518
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33300.039.2288481
TCAACGC35700.035.25784
GTACATG94700.034.661321
GGTATCA20150.034.1829871
CAACGCA37950.033.2925765
TACATGG96950.033.0723532
ATCAACG38750.032.359513
AACGCAG40450.031.2349416
TATCAAC41700.030.8704092
ACATGGG103350.030.1942713
CATGGGG75050.025.1256544
ACGCAGA48700.025.0658077
CGCAGAG51750.023.4049328
AGTACAT63050.022.2252222
GAGTACA67450.021.9730191
GTATAGC8450.020.799941
GGACCGT5950.019.1589576
CATGGGA36750.018.8700524
GTGTAGC17250.018.7256471
ATGGGGG39050.018.123535
Produced by FastQC (version 0.11.5)