FastQCFastQC Report
Fri 10 Feb 2017
SRR2568649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749518
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30600.035.768581
GTACATG84700.031.8000891
TCAACGC33050.031.3429494
GGTATCA19650.031.2408241
TACATGG87350.030.6118492
CAACGCA34450.030.207155
ACATGGG89600.029.0621283
AACGCAG36700.028.2257356
ATCAACG37300.027.8990963
TATCAAC40950.025.5614222
CATGGGG63600.024.0576734
GTATATA7900.023.4926951
GAGTACA63350.022.3854061
AGTACAT58350.022.0975742
ACGCAGA46050.021.9784457
TACCGTA4250.021.2427947
CGCAGAG48300.020.5524888
GTACCGT6800.018.8672986
ACCGTAT5600.018.6594968
ATGGGGG34250.018.4521055
Produced by FastQC (version 0.11.5)