FastQCFastQC Report
Fri 10 Feb 2017
SRR2568648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5073520
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72650.038.314531
GGTATCA44250.036.7125361
TCAACGC81450.033.2985344
CAACGCA84450.032.1718865
ATCAACG86550.031.3364033
AACGCAG87750.031.1243926
TATCAAC93100.029.4393142
GTACATG169400.028.9940361
TACATGG168950.028.2840332
ACATGGG180850.026.1064623
ACGCAGA104350.025.62697
CATGGGG114250.024.736654
CGCAGAG109950.023.9328638
ATGGGGG58200.019.260575
GTATAGC15300.019.2487181
AGAGTAC107500.019.10968410-11
GTATAGA13500.018.6484891
GTATATA28850.018.6051921
GCAGAGT138450.018.3544319
GTATTAG17150.016.618381
Produced by FastQC (version 0.11.5)