FastQCFastQC Report
Fri 10 Feb 2017
SRR2568648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5073520
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT56490.11134281524464278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71800.038.2321781
GGTATCA41300.035.3643761
TCAACGC79400.033.3903964
CAACGCA80100.033.039285
ATCAACG82250.032.2911723
AACGCAG84400.031.4122926
TATCAAC89550.030.0117912
GTACATG155750.028.0103781
TACATGG154950.027.1067962
ACGCAGA101250.025.5261947
ACATGGG163900.024.6114183
CGCAGAG104900.024.3600238
CATGGGG99550.022.8613644
AGAGTAC105400.018.83677710-11
GCAGAGT132350.018.6257749
TACCGTA5800.018.0209167
ACCGTAT7150.017.9361258
ATATAAG23200.017.430451
ATGGGGG48800.017.0382965
TATACAG33700.016.7774125
Produced by FastQC (version 0.11.5)